ISH19

  • Family IS5
  • Group ISH1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006393 ND Haloarcula marismortui
Haloarcula marismortui ATCC 43049 plasmid pNG500
DNA section
IS Length : 936 bp

Ends


IR Length : 17/21

IRL : GGCTCTGGTGAATCGCGGGACTGCGTCGGTTTCGACCGTTAGAACTAGTG
IRR : GGCCGTGGTGAATCGCCAGACATAGCTTGATTCCACGGCTACAGAGTCTC

Insertion site


Left flankDirect repeatRight flankDR Length
TGCCGGTAGTCCTACCGATGGAAAACACCCCACCG7

DNA sequence

GGCTCTGGTGAATCGCGGGACTGCGTCGGTTTCGACCGTTAGAACTAGTGGTATGAAGCGGGAAACCGCCGATGCTCTATGTCGAAGATCTCCCGCTTCA
CGAAGAAAGCAGTCCAGTTAGCTAAAAATGCTGTTGGTGAGCGAGGCGAAGTCGCCGCCCCCGAAGGGGGTGGCGGCTTCGCCGAGTATGCGGTGGTGTC
GCTGCACTGTCTGCGGGTTTACCTGGAAAAATCCTACCGAGTAGCACTTGATTTGCTGAGTGAGATGCCACAGATACTCGGGGAGATCGGCCTCGACGCG
GCCGATCTCCCCGACCACTCCACGCTAGTCAAGTGGTTTGACAGAATCAAGACTGCACTCTGGCGAGTGCTGCTGCGCCTGTCGGCGCAGGAGCACGAGC
CATCTGGTCACGCCGCTATCGATGCGACATTTTTCGACCGCGAAAACGCTAGCAAACACTACTGCCGCCGGACGAATTACCGGGTTCAGACGCTCAAAGC
GACAGCTCTCGTCGACACAGAAAGCCAAGCCATTCTAGACGTGCACTGTACGACTGAGAAACGCCACGACACGCAACTCGGCTGGCAGGTCGCCCGCGCA
ACGCGGCGACCTCGCCAGCCTCGCTGCCGACAAAGGCTACGATTGGATGGATTTACGCGAAAAACTCCGCGAAGAGGGCGTAAGACCCCTGATCAAACAC
CGTGAGTTCCGGCCCATCGATCACGCGCACAACGCGCGGATCGATGGGCCTCGATACCGCCAACGAGCGATGTGTGAGACCGTCTTCTCCACAATCAAGC
GCACGCTCGGCGACGCCGTGCGTGCGCGAACTTGGTACGGTGAGTTTCGTGAACTCGTTCTGATGTGTGTCGTTCACAACATCAAGGAGACTCTGTAGCC
GTGGAATCAAGCTATGTCTGGCGATTCACCACGGCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
820 bp273 aa79898+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKISRFTKKAVQLAKNAVGERGEVAAPEGGGGFAEYAVVSLHCLRVYLEKSYRVALDLLSEMPQILGEIGLDAADLPDHSTLVKWFDRIKTALWRVLLR
LSAQEHEPSGHAAIDATFFDRENASKHYCRRTNYRVQTLKATALVDTESQAILDVHCTTEKRHDTQLGWQVARATRGDLASLAADKGYDWMDLREKLREE
GVRPLIKHREFRPIDHAHNARIDGPRYRQRAMCETVFSTIKRTLGDAVRARTWYGEFRELVLMCVVHNIKETL

 

Blast result :
Comments
ISH19 is 93% aa similar to ISH9. There is a premature STOP codon and a frameshift. The transposase is probably inactive, it was reconstructed in silico.
References
1] Baliga,N.S., Bonneau,R., Facciotti,M.T., Pan,M., Glusman,G., Deutsch,E.W., Shannon,P., Chiu,Y., Weng,R.S., Gan,R.R., Hung,P., Date,S.V., Marcotte,E., Hood,L. and Ng,W.V. (2004) Genome Res. 14 (11), 2221-2234