ISMba19
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007355 | ND | Methanosarcina barkeri | Methanosarcina barkeri fusaro |
DNA section
IS Length : 858 bp
Ends
IR Length : 15
IRL : GAGCCTATCCCAAAATTAATATTATGCTGAAATTATAAATTTACTTTTAT
IRR : GAGCCTATCCCAAAAGTTCATTTAATCTAATTATTGCTGCTATCATTACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GAGCCTATCCCAAAATTAATATTATGCTGAAATTATAAATTTACTTTTATGTCCTTTCGTGAAATCGATGACGTTCTATGGAACTCCATAGAACCTTATC
TTCCTCCACAGAAACCACATACAGGAAGGCCGCGTGCAAATATGAGGAAGTTAATGAACGGTATTTTTTACGTTGTTATGACTGGTTGTACGTGGAAAGA
CGTTCCCAGGAGATATGGATCTAAGTCAACAGTTCATAGATTTCATCTATATCTGTGTGAACATGGTATCTATCCGAAGATTTTCAATGAGCTTTTAAAC
AAAGGTTACGACTTGAAGAAAATAGATCTTTCTCATTGCTTTACTGATACAAAGGATATTCCGGCTAAAAAAGGGGAAATATCGGCTACGATGGATACAA
AAAAATAAAAAGAATAAAAATAAGCGTTTTAGTAGACTTACAGGGTTTACCTCTCTCGATTATCATTGTTCCTGCAAATAAAAATGATTCTACACTTTAT
ATACCTACACTTAAAAATTTCAAGATAAAAAGACCTATAGGAAGGCCTGTTAACAGGCCTTCCAAAGTAACAGCTGATGCAATGTATGATACAGCTAAAA
TTAGAAAATATAACAGGAGAAGAGGAATAAAGTCCAATATACTAGTAAATAAAAGAAATAGGAAAAATAAGAAGAGAGGAAGACCAATAAAGGTAGATCA
GGAAGAATACAAAAAGAAAAGCGTAGTAGAAAGGTTCTTTAGTTGGATAGAGTCATGCAAGAAAGTATTTCCGAGATATGAAATTAAAGAGACATCATAT
TTAGGAGTTGTAATGATAGCAGCAATAATTAGATTAAATGAACTTTTGGGATAGGCTC
TTCCTCCACAGAAACCACATACAGGAAGGCCGCGTGCAAATATGAGGAAGTTAATGAACGGTATTTTTTACGTTGTTATGACTGGTTGTACGTGGAAAGA
CGTTCCCAGGAGATATGGATCTAAGTCAACAGTTCATAGATTTCATCTATATCTGTGTGAACATGGTATCTATCCGAAGATTTTCAATGAGCTTTTAAAC
AAAGGTTACGACTTGAAGAAAATAGATCTTTCTCATTGCTTTACTGATACAAAGGATATTCCGGCTAAAAAAGGGGAAATATCGGCTACGATGGATACAA
AAAAATAAAAAGAATAAAAATAAGCGTTTTAGTAGACTTACAGGGTTTACCTCTCTCGATTATCATTGTTCCTGCAAATAAAAATGATTCTACACTTTAT
ATACCTACACTTAAAAATTTCAAGATAAAAAGACCTATAGGAAGGCCTGTTAACAGGCCTTCCAAAGTAACAGCTGATGCAATGTATGATACAGCTAAAA
TTAGAAAATATAACAGGAGAAGAGGAATAAAGTCCAATATACTAGTAAATAAAAGAAATAGGAAAAATAAGAAGAGAGGAAGACCAATAAAGGTAGATCA
GGAAGAATACAAAAAGAAAAGCGTAGTAGAAAGGTTCTTTAGTTGGATAGAGTCATGCAAGAAAGTATTTCCGAGATATGAAATTAAAGAGACATCATAT
TTAGGAGTTGTAATGATAGCAGCAATAATTAGATTAAATGAACTTTTGGGATAGGCTC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 360 bp | 119 aa | 192 | 551 | + | No |
Description : First part of the transposase
ORF sequence :
MSFREIDDVLWNSIEPYLPPQKPHTGRPRANMRKLMNGIFYVVMTGCTWKDVPRRYGSKSTVHRFHLYLCEHGIYPKIFNELLNKGYDLKKIDLSHCFTD
TKDIPAKKGEISATMDTKK
TKDIPAKKGEISATMDTKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 273 bp | 90 aa | 725 | 997 | + | No |
Description : Second part of the transposase
ORF sequence :
MYDTAKIRKYNRRRGIKSNILVNKRNRKNKKRGRPIKVDQEEYKKKSVVERFFSWIESCKKVFPRYEIKETSYLGVVMIAAIIRLNELLG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 806 bp | 0 aa | 192 | 997 | + | Yes |
Chemistry : DDE
ORF sequence :
Blast result :
Comments
ISMba19 is 73% (ORF A) aa similar to ISMma12 and 60% (ORF B) to IS1650.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goodwin,L.A., Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Anderson,I. and Richardson,P. (2005) Direct submission GenBank