ISMma22

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003901 ND Methanosarcina mazei
Methanosarcina mazei Go1
DNA section
IS Length : 1658 bp

Ends


Left end : AAGCAAGTAAGGGCTAATCCCCGCCATTTCAATGGTGGGATACAGCCCGTCAACTTCCTAACATAATAGCGTTAAATTAATATACACATGGTTACCATAT II struct. : Yes
Right end : GATAGAGATCACAATGCCGCTATTAACATTATGAGATTGGGATTACAATCTCTCCAGAATTCTGGTAGATGCCCGTCACTTCAGTGATGGGAGTAGTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TATTATTCATTTATCCCCTTTCTATCAC

DNA sequence

AAGCAAGTAAGGGCTAATCCCCGCCATTTCAATGGTGGGATACAGCCCGTCAACTTCCTAACATAATAGCGTTAAATTAATATACACATGGTTACCATAT
GTATATACTAACATGGCAGGAAAAGAACATTGGATAAGTGCAAGAGGTTGTGTATATAACACAAATTACCATTTCGTATGGTCTACAAAATATAGAAGAA
AAATCCTTATTGGAAAAATAGCTGAAGATCTGAAAGAGCTTCACGAACAGATAGCAAAAGAAAAAGGTGTAACTCTTGTCACTCAAGAAGTAATGCCGGA
TCATGTGCATCTTTTCATTATCATGCATCCCAAGTTTGCACCTGCTGATATTGTGAAAATATTCAAAGGAATTACTGCAAAGAAACTCTTTGAAATGCAT
CCTGAAATAAAGAGCAGATTATGGAATGGTCATCTCTGGAATCCGTCTTATTATGTTGGAACATGTGGAGATACAACAAAAGAAACTGTAAAAATGTATA
TTGAAACTCAAAAGGTGAAATGAATGTTAAAAGTGCTTCGGTATAGAGTCTATCCTACAAAGGCTCAAAAAACAACTATGAATAATACTCTTGAGCTTTG
TAGGTGGACATATAACGAGACACTTGCACTCAGGAAAGATGCATGGGAGTCAGAGCAAAAGAATATAGGGTACTATGATTCTAAGAAAATGATCCCTGAA
TGGAAAAAAGAAAAACCTGAATTGAAAACAGTTCATTCACAGGTATTACAGGAAGTAATCAAGAGAGTAGATCTTGCCTTTCAAGCCTTTTTCCGTAGAG
TTAAAACAGGAGAGAAGGCAGGCTATCCTAGATTCAGAGGATACGGAAGGTATGATAGTTTTACCTATACTCAATCAGGCTTTTCTCTGGATTCTGATAG
GTTAACACTATCAAAAATAGGTGATATTAAAATCAAATTACATAGACCAGTAGAAGGTGAAATTAAAAGACTCAATGTTCGTAAGATGCCTACAGGAAAA
TGGTTTGTTTCTTTTCTGGTTGAAACTGATACTCCTTTGGAATTACAAAAAACAGGATTATCAGTAGGTGTAGACGTAGGTATCAAGAGCTTCTTAACGC
TTTCTGATGGTAATTATGTACCTAATCCTAGATTCTTTGTTACAGAAGAAAAGTTTCTGGCAAAAGTCCAAAGAAAACTCTCCAAACTTGAAAAAGGAAC
ACCTGAAAGAGCAAAAGCCTTGAAAGCTGTTCAACGTGTTCACGAAAGAATCTCAAACAAGAGAGAGGATTTTGTACAGAAAGTAAGTCTTAATCTGGTC
AGGAATTATGACCTGATTACTTTTGAAGATTTGAACATTAAAGGTATGATTAAAAACCACAAACTAGCAAAACACATAGCTGATGTTGCATGGAATAAGC
TTGTAACTATCACGTCTTACAAGGCAGAATGGGCTGGTAAACGTGTAGAGCTTGTCAATCCATGTAATACATCACAAATGTGCTCTGGATGCGGTGAAAT
AGTTAAAAAAGAACTATCAGAGAGAATACACAGTTGTCCTTACTGTGGTCTTACTCTTGATAGAGATCACAATGCCGCTATTAACATTATGAGATTGGGA
TTACAATCTCTCCAGAATTCTGGTAGATGCCCGTCACTTCAGTGATGGGAGTAGTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
411 bp136 aa113523+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MAGKEHWISARGCVYNTNYHFVWSTKYRRKILIGKIAEDLKELHEQIAKEKGVTLVTQEVMPDHVHLFIIMHPKFAPADIVKIFKGITAKKLFEMHPEIK
SRLWNGHLWNPSYYVGTCGDTTKETVKMYIETQKVK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1122 bp373 aa5241645+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLKVLRYRVYPTKAQKTTMNNTLELCRWTYNETLALRKDAWESEQKNIGYYDSKKMIPEWKKEKPELKTVHSQVLQEVIKRVDLAFQAFFRRVKTGEKAG
YPRFRGYGRYDSFTYTQSGFSLDSDRLTLSKIGDIKIKLHRPVEGEIKRLNVRKMPTGKWFVSFLVETDTPLELQKTGLSVGVDVGIKSFLTLSDGNYVP
NPRFFVTEEKFLAKVQRKLSKLEKGTPERAKALKAVQRVHERISNKREDFVQKVSLNLVRNYDLITFEDLNIKGMIKNHKLAKHIADVAWNKLVTITSYK
AEWAGKRVELVNPCNTSQMCSGCGEIVKKELSERIHSCPYCGLTLDRDHNAAINIMRLGLQSLQNSGRCPSLQ

 

Blast result :
Comments
ISMma22 is 67% (ORF A) aa similar to ISDra2 and 56% (ORF B) to ISMac7.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C., Bruggemann,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G. (2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461