ISMma19

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003901 ND Methanosarcina mazei
Methanosarcina mazei Go1
DNA section
IS Length : 1708 bp

Ends


Left end : ATGACGAAGCGTAAAACCCCTGAGTCTTTAGCTCAGGGGATATAAGCGTCAACTTCAACCCTGATTCCGTATGTAATATTCTGCCGCCTCTTTACTAACG II struct. : Yes
Right end : CACCTGCGGAACGTGGGGAAGAGCTTGGGGACTTGCCCTCAATAGAGGGAGGAATGAACCAAGAAGCCCATTAAATCTTTAGATTTAGTGGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
ATTTTGGAAATTTATTTTCCGGACTtcac

DNA sequence

ATGACGAAGCGTAAAACCCCTGAGTCTTTAGCTCAGGGGATATAAGCGTCAACTTCAACCCTGATTCCGTATGTAATATTCTGCCGCCTCTTTACTAACG
TTTCCGATAGTAGACGCAAAATATCCATCACTCCATAACGTATTTTCACCCCAATAATACTTTTCCAGATAATCTTTTTGAGTTTTCCACAATCGGTTTG
TCGATTCTTGTTTCAATTTTCTGACAATTGACAAAACACTAACTTTCGGTTCACTCTTAATCAGGAAATGGATATGGTCTTTGTCTGTTTCCATTTCAAG
GATTTCAAAGTTTGACTCTTTTGAAATGTCAATCATAATCTGTTTTAGTTCTTCGCTAATCGGTTCAAGTATGACTTTTCGGTATTTGCAAACAAAAATA
ACATGGTACATTAACAAAAATTTTCTATGATTCCGTGTTTCATACTTCATATTTACTAAAAAGTACATATGTTGTTAAAGAGCATAATACCTTGTATGGT
GAAAGCGTTCAAATTTAGACTCTATCCTACAACTGCACAAGCTGTTCAATTGAATCAGCATATAGGTAGCTGTAGATTTGTCTACAATTGGGCGTTGGAT
CAAAAAATTAAAACTTACGAACAAACAGGAAAATCAATTTCCAGATTTGACTTAAACAAATTAATTCCTACTCTAAAGGCTTCTAATGAATGGTTAGGTG
AAGTCAACTCTCAATCATTACAGGGGATGACTAAGCAGGTTGAATCTGCTTTCACTCGGTTTTTTAGAGGGAAGACCGGATTCCCTAAGTTTAAGTCTAA
GAAAAATCCTATACAATCTTTCCCTGTACCTCAGCACTACTCCGTAGACTTTGAAAAAAACACTATTAAGCTTCCTAAAATAGAACCAATTAAAGCAGTT
TTTCACCGGAAGTTTGAAGGAGAACTTAAAACAGCTACGGTATCAAGGACTTGTAAAGGGCATTACTATATCAGTATCCTTGTTGAAGATGGTAAAGAAC
TTCCAGTAAAGGAAGCTTTCGCAGAATCAACAACAATAGGAATTGATGTAGGTATCAAAGATTTTGCAGTTCTTTCTAATGGAGAAAAAATTGAAAATCC
TAAGTACCTGAAAAACTCTCTTAAGAGGCTTAAAGTTCTTCAGAAAAGAGTTTCGAAGAAACAAAAAGGCTCTAAGAATAGGGCAAAAGCTAAACAAAGA
CTTGCTGTACTCCATGATAAAATAACTAATCAGAGAAATGATTTCCAGAACAAACTCTCTTTTAAACTGATAAGCGAAAACCAAGCTGTAGCTCTGGAAA
CATTAAATGTTAAAGGCATGGTTAAGAATCATCATTTAGCACAGTCTATTAGTGATTCTGCATGGAGTAGTTTTGTAACAAAGTTAGAATATAAGGCTGA
ATGGTTCGGGAAAACTATCTTAAGAATCGGACAATTTGAACCATCTTCCAAGCTCTGTAGTGTATGTGGATACCACAATAAAGAGCTTCAGTTAAAAGAC
AGGGAATGGACTTGTCCTGACTGTAAAACAAAACATGATAGAGACATTAATGCCGCTATCAATATCAAGAAATTTGCTCTCGTAGATCAAAATCTAATTG
GATTGTAACACCTGCGGAACGTGGGGAAGAGCTTGGGGACTTGCCCTCAATAGAGGGAGGAATGAACCAAGAAGCCCATTAAATCTTTAGATTTAGTGGG
TAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
414 bp137 aa46855-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MYFLVNMKYETRNHRKFLLMYHVIFVCKYRKVILEPISEELKQIMIDISKESNFEILEMETDKDHIHFLIKSEPKVSVLSIVRKLKQESTNRLWKTQKDY
LEKYYWGENTLWSDGYFASTIGNVSKEAAEYYIRNQG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1140 bp379 aa4691608+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLLKSIIPCMVKAFKFRLYPTTAQAVQLNQHIGSCRFVYNWALDQKIKTYEQTGKSISRFDLNKLIPTLKASNEWLGEVNSQSLQGMTKQVESAFTRFFR
GKTGFPKFKSKKNPIQSFPVPQHYSVDFEKNTIKLPKIEPIKAVFHRKFEGELKTATVSRTCKGHYYISILVEDGKELPVKEAFAESTTIGIDVGIKDFA
VLSNGEKIENPKYLKNSLKRLKVLQKRVSKKQKGSKNRAKAKQRLAVLHDKITNQRNDFQNKLSFKLISENQAVALETLNVKGMVKNHHLAQSISDSAWS
SFVTKLEYKAEWFGKTILRIGQFEPSSKLCSVCGYHNKELQLKDREWTCPDCKTKHDRDINAAINIKKFALVDQNLIGL

 

Blast result :
Comments
ISMma19 is 93% (ORF A) AND 95% (ORF B) aa similar to ISMac7.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C., Bruggemann,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G. (2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461