ISMma9

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003901 ND Methanosarcina mazei
Methanosarcina mazei Go1
DNA section
IS Length : 1200 bp

Ends


IR Length : 15/22

IRL : TGTCGTGTCACCGATGGAAAGTCTTAAGCGTTATATTGAATGATCTTCAT
IRR : TGTCGCGTCAATCCTCAATAGTCGGATAAAGACGTGACAATTTTATCCTT

Insertion site


Left flankDirect repeatRight flankDR Length
CCTTAAGAGACTAAGCTATGCAGG4
TTACAACCGAGTAGCCTGTTTTTC4
TGACGCGACACTAGCTTCATTCTT4
CTTAAAATTACTAGTAACGAATCT4
TACTTCAAGGCTAGTACAATTTCA4
AGGAAAAACACTAGTGAGTTACAG4

DNA sequence

TGTCGTGTCACCGATGGAAAGTCTTAAGCGTTATATTGAATGATCTTCATAAGTATCATTCATGATTAAATATACTGTGACACTTACCGAAGATGAACGT
AGATCTCTTTGTGAACTTGCTTCAAAGGGAAAACATAACTCTCAACAAATTCTCAATGCTCTGATTTTGCTTAATTGCGATAAAAGTGAATTGAATGTTA
GCCATTCAACCAATGAAGAAATCTCACGTGTCCTGAATATTAGTATGAAAAAAATTGACCGTGTTAAGAAGAGATTTGTTGAAGAAGGTCTTGAAGTTGC
CCTTAACGGGAAAGAAAGCGAGCGCATTTATAATAAAAAAGTTGATGGTGATCTTGAAGCCCATTTAGTCGCCCTTAGTTGCAGTCAACCCCCTGAAGGT
TTTGCAAGATGGTCGTTAAGATTATTAGCCGATAAGGCTGTAGAACTTGGTTATTTCGAGGAAATTTCTCACGAAACAGTACGCCGTACTTTAAAAAAAC
GAAATCAAACCCTGGCAAAGGAAACAATGGGTAATTCCTCCAGAACAAAACAGTAGCTTTGTTGCGAATATGGAAATGGTTCTGGACGTTTATAAACGTC
CATTTGATCCACGAAATCCTGTTGTGTGTATGGACGAATCCCCAAAACAATTGATTGCAGAAACCCGGATCCCTATCCCCTGCTCAACAGGGAAGCCAGA
AAAGTACGATTATGAATACAAGCGAAATGGAATGTGCAATATATTCCTTGCCTGTGAACCGTTGACAGGGAAAAGAATGGTAAAGATCACTGAAAGAAAA
ACAAAGAGAGATTGGGCTTATTTTCTCGAAGAAATAGAAGTTCAACATGAAAATGCAGATAAAATTACGTTAGTAATGGACAATCTAAACACGCACATAC
CTGGATCTCTCTACGAAACATTTCCACCACCAAAAGCAAAGGCGCTATGGGACAGATTTGAGTTCGTCTACACGCCAAAACATGGAAGCTGGTTGAACAT
GGCAGAGATTGAACTGAATGTACTTACAGGTCAATGTTTAAAAAGGAGAATGGATAACATCGAGTTTGTCAGAAAAGAAGTTTTGGCATGGCAAAATTAC
AGAAACAACAAAAATTCAAAGGTTAAATGGCAATTTACAACAGATGATGCAAGGATAAAATTGTCACGTCTTTATCCGACTATTGAGGATTGACGCGACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
495 bp164 aa62556+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MIKYTVTLTEDERRSLCELASKGKHNSQQILNALILLNCDKSELNVSHSTNEEISRVLNISMKKIDRVKKRFVEEGLEVALNGKESERIYNKKVDGDLEA
HLVALSCSQPPEGFARWSLRLLADKAVELGYFEEISHETVRRTLKKRNQTLAKETMGNSSRTKQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
624 bp207 aa5701193+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MEMVLDVYKRPFDPRNPVVCMDESPKQLIAETRIPIPCSTGKPEKYDYEYKRNGMCNIFLACEPLTGKRMVKITERKTKRDWAYFLEEIEVQHENADKIT
LVMDNLNTHIPGSLYETFPPPKAKALWDRFEFVYTPKHGSWLNMAEIELNVLTGQCLKRRMDNIEFVRKEVLAWQNYRNNKNSKVKWQFTTDDARIKLSR
LYPTIED

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1132 bp377 aa621193+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIKYTVTLTEDERRSLCELASKGKHNSQQILNALILLNCDKSELNVSHSTNEEISRVLNISMKKIDRVKKRFVEEGLEVALNGKESERIYNKKVDGDLEA
HLVALSCSQPPEGFARWSLRLLADKAVELGYFEEISHETVRRTLKKNEIKPWQRKQWVIPPEQNSSFVANMEMVLDVYKRPFDPRNPVVCMDESPKQLIA
ETRIPIPCSTGKPEKYDYEYKRNGMCNIFLACEPLTGKRMVKITERKTKRDWAYFLEEIEVQHENADKITLVMDNLNTHIPGSLYETFPPPKAKALWDRF
EFVYTPKHGSWLNMAEIELNVLTGQCLKRRMDNIEFVRKEVLAWQNYRNNKNSKVKWQFTTDDARIKLSRLYPTIED

 

Blast result :
Comments
ISMma9 is 62% aa similar to ISMth1.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baeumer,S., Jacobi,C., Brueggemann,H., Lienard,T., Christmann,A., Boemecke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.-P., Gunsalus,R.P., Fritz,H.-J. and Gottschalk,G. (2002)J. Mol. Microbiol. Biotechnol.