ISSac1
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007759 | ND | Syntrophus aciditrophicus | Syntrophus aciditrophicus |
DNA section
IS Length : 1217 bp
Ends
IR Length : 16/22
IRL : CCACGTAACATATTATAGTTCGCTTTTGTATAAAGTGTGATACACTGGTG
IRR : CCACGTAACTTCTATAAATTCGGATAAAGACGTTTCAGCTTGATCCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAATGTAAGAACGTAT | CTAGTA | ATCATTGTCATAAAA | 6 |
DNA sequence
CCACGTAACATATTATAGTTCGCTTTTGTATAAAGTGTGATACACTGGTGACACCATGATAAAGGAGGTCGCCATGTCACCAAGATATCGAGTAACATTG
ACCCAGGATGATCGTAAAGAATTGGAGGCATTAACACGACGTGGAAAAACACACGCGAGAAGAGTTATACATGCCCGGGCTTTATTGCTTTGTGACGCCG
GAATTGATGGGCCGGCATGGAATGTTGCCGACATTGCAAAGGCGCTTGGAATAACAAGCCGCACGATTGAACATTTAAAAAAGCGGTTTGTAACGGATGG
CCTTGAATCTGCGATGGAGCGCAAACCACGGGAAAAACCGCCAAGAGAAATCTTGTTTGACGGTGCATTTGAAGCAAGGCTGATCGCCTTGGCTTGCTCA
GAGGCTCCCGAGGGACACCAACGATGGACAGTCAGACTTCTTGCCGATAAGGTTGTCGAGCTGAACATCGCTCCATCCATATCGCATATGACTGTGCAGC
GGATTTTAAAAAAAACGAACTTAGACCTCACCTCAGCAAGTACTGGAAAATCCCGCCGAAAGGAAGCGCAGCATTTGTAGCCGCGATGGAGGATCTTTTG
GAAGTCTACCATCTGCCATATGATGCTGATTATCCGGTGGTATGCATGGACGAGTCTTGCAAGCAAATGATCGGAGAAGTCCGTGAGCCGATACCGTGCA
AACCGGGACAACCGGTGCGCGTTGACGACGAATACGTCAGAAACGGCGTCGCAGAAATATTCATGGAAGTGGAACCGTTGGCGGGCAAAAGACATGTGGC
GGTGACGGAACGCCGCACCAGGAAGGATTGGGCGTTGCAGATCAAACAGATGCTTGATGAACGCTATCCTGACGCAATCAAAGTGCGACTGGTTATGGAC
AATTTGAATACGCACAATATCGCCTCACTCTATGAAACATTTGAGCCCAAGGAGGCCAGACGCCTGGCGGAACGGCTTGACATCCATTATACGCCGAAGC
ATGGAAGCTGGCTCAACATGGCAGAAATTGAACTCAGCGTTCTGAATGGTCAATGCCTTGATCGACGGATCGCCGACATGGAAACCATGCAGGCCGAAGT
GGATGCATGGGAAAGAGACCGAAACAACAGCTCCAAAATAATTGATTGGCAATTTACTACCTCAGACGCTCGGATCAAGCTGAAACGTCTTTATCCGAAT
TTATAGAAGTTACGTGG
ACCCAGGATGATCGTAAAGAATTGGAGGCATTAACACGACGTGGAAAAACACACGCGAGAAGAGTTATACATGCCCGGGCTTTATTGCTTTGTGACGCCG
GAATTGATGGGCCGGCATGGAATGTTGCCGACATTGCAAAGGCGCTTGGAATAACAAGCCGCACGATTGAACATTTAAAAAAGCGGTTTGTAACGGATGG
CCTTGAATCTGCGATGGAGCGCAAACCACGGGAAAAACCGCCAAGAGAAATCTTGTTTGACGGTGCATTTGAAGCAAGGCTGATCGCCTTGGCTTGCTCA
GAGGCTCCCGAGGGACACCAACGATGGACAGTCAGACTTCTTGCCGATAAGGTTGTCGAGCTGAACATCGCTCCATCCATATCGCATATGACTGTGCAGC
GGATTTTAAAAAAAACGAACTTAGACCTCACCTCAGCAAGTACTGGAAAATCCCGCCGAAAGGAAGCGCAGCATTTGTAGCCGCGATGGAGGATCTTTTG
GAAGTCTACCATCTGCCATATGATGCTGATTATCCGGTGGTATGCATGGACGAGTCTTGCAAGCAAATGATCGGAGAAGTCCGTGAGCCGATACCGTGCA
AACCGGGACAACCGGTGCGCGTTGACGACGAATACGTCAGAAACGGCGTCGCAGAAATATTCATGGAAGTGGAACCGTTGGCGGGCAAAAGACATGTGGC
GGTGACGGAACGCCGCACCAGGAAGGATTGGGCGTTGCAGATCAAACAGATGCTTGATGAACGCTATCCTGACGCAATCAAAGTGCGACTGGTTATGGAC
AATTTGAATACGCACAATATCGCCTCACTCTATGAAACATTTGAGCCCAAGGAGGCCAGACGCCTGGCGGAACGGCTTGACATCCATTATACGCCGAAGC
ATGGAAGCTGGCTCAACATGGCAGAAATTGAACTCAGCGTTCTGAATGGTCAATGCCTTGATCGACGGATCGCCGACATGGAAACCATGCAGGCCGAAGT
GGATGCATGGGAAAGAGACCGAAACAACAGCTCCAAAATAATTGATTGGCAATTTACTACCTCAGACGCTCGGATCAAGCTGAAACGTCTTTATCCGAAT
TTATAGAAGTTACGTGG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 174 aa | 56 | 580 | + | No |
Description : First part of the transposase
ORF sequence :
MIKEVAMSPRYRVTLTQDDRKELEALTRRGKTHARRVIHARALLLCDAGIDGPAWNVADIAKALGITSRTIEHLKKRFVTDGLESAMERKPREKPPREIL
FDGAFEARLIALACSEAPEGHQRWTVRLLADKVVELNIAPSISHMTVQRILKKTNLDLTSASTGKSRRKEAQHL
FDGAFEARLIALACSEAPEGHQRWTVRLLADKVVELNIAPSISHMTVQRILKKTNLDLTSASTGKSRRKEAQHL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 627 bp | 206 aa | 586 | 1212 | + | No |
Description : Second part of the transposase
ORF sequence :
MEDLLEVYHLPYDADYPVVCMDESCKQMIGEVREPIPCKPGQPVRVDDEYVRNGVAEIFMEVEPLAGKRHVAVTERRTRKDWALQIKQMLDERYPDAIKV
RLVMDNLNTHNIASLYETFEPKEARRLAERLDIHYTPKHGSWLNMAEIELSVLNGQCLDRRIADMETMQAEVDAWERDRNNSSKIIDWQFTTSDARIKLK
RLYPNL
RLVMDNLNTHNIASLYETFEPKEARRLAERLDIHYTPKHGSWLNMAEIELSVLNGQCLDRRIADMETMQAEVDAWERDRNNSSKIIDWQFTTSDARIKLK
RLYPNL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1157 bp | 383 aa | 56 | 1212 | + | Yes |
Chemistry : DDE
ORF sequence :
MIKEVAMSPRYRVTLTQDDRKELEALTRRGKTHARRVIHARALLLCDAGIDGPAWNVADIAKALGITSRTIEHLKKRFVTDGLESAMERKPREKPPREIL
FDGAFEARLIALACSEAPEGHQRWTVRLLADKVVELNIAPSISHMTVQRILKKNELRPHLSKYWKIPPKGSAAFVAAMEDLLEVYHLPYDADYPVVCMDE
SCKQMIGEVREPIPCKPGQPVRVDDEYVRNGVAEIFMEVEPLAGKRHVAVTERRTRKDWALQIKQMLDERYPDAIKVRLVMDNLNTHNIASLYETFEPKE
ARRLAERLDIHYTPKHGSWLNMAEIELSVLNGQCLDRRIADMETMQAEVDAWERDRNNSSKIIDWQFTTSDARIKLKRLYPNL
FDGAFEARLIALACSEAPEGHQRWTVRLLADKVVELNIAPSISHMTVQRILKKNELRPHLSKYWKIPPKGSAAFVAAMEDLLEVYHLPYDADYPVVCMDE
SCKQMIGEVREPIPCKPGQPVRVDDEYVRNGVAEIFMEVEPLAGKRHVAVTERRTRKDWALQIKQMLDERYPDAIKVRLVMDNLNTHNIASLYETFEPKE
ARRLAERLDIHYTPKHGSWLNMAEIELSVLNGQCLDRRIADMETMQAEVDAWERDRNNSSKIIDWQFTTSDARIKLKRLYPNL
Blast result :
Comments
ISSac1 is 61% aa similar to ISMth1.
References
1] Gunsalus,R., Rohlin,L., Kim,U., Krupp,R., Bhattacharyya,A., Campbell,J., Mclerney,M., Moutakki,H. and Rio-Hernandez,L. (2006)