ISArch4
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY714840 | ND | Uncultured archaeon | Uncultured archaeon GZfos26D6 |
DNA section
IS Length : 1212 bp
Ends
IR Length : 19/24
IRL : TGTCGTGTCAACCCTATAATGTCGTAAGTTATATAATGTATGTTCTTCAT
IRR : TGTCGTGTCAACTTTCAAGTGTCGGATAAAGGCGAGATAACTTTATCCGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATATTCTTCAAATGTT | CTAG | CTTCTGGATAAGGT | 4 |
DNA sequence
TGTCGTGTCAACCCTATAATGTCGTAAGTTATATAATGTATGTTCTTCATAATTAATCTAGGAGGATTGAATATGAAGAAATATATCGTGGCACTTGCCA
AAGATGAGCGCGAAACTCTTGGCGCACTTACTTCCAAAGGTAAACATAAATCACAGAAAATCCTTAACGCACTGATTTTATTGGGGTGCGATGAGGGGGA
GTATCAAAAGAAACGTTCGACCAATGAAGAAATGGCTCGCATCCTGAACATAAGTATGAAAAAGATCGATCGTGTGAAGAAACGATTTGTCATGGACGGT
ATCGAGGTTGCCCTTAACGGGAGAAAGGGAAGCAGTATCTACGCTAAAAAAGTGGATGGCGATTTTGAAGCCCATTTGGTTGCAGTGAGTTGCAGTGAGC
CACCCGAAGGCTTTGCGAGATGGTCTCTGAGGTTGTTAGCCGACAAGGTTGTTGAGCTGGATTATATTGATAGCATTTCCCATGAAACGGTACGCCGTAT
CCTAAAAAAAACGAAATCAAACCCTGGCAACGAAAAGGATGGGTAATTCCTCCAGAGCAAAACGGTAACTTTGTTGCGAATATGGAAATGGTGTTGGATG
TTTACAAGCGTCCGTTTGATCCACTATATCCCGTTCTAGGCATGGACGAATCCCCAAAGCAGCTGATTGCAGAGACAAGAACGCCCATTCCTGCTTCGCC
CGGGCAGCCGACCAAACATGATTATGAATATAGGCGTTGCGGCATGTGCAATATTTTTCTCGCCTGTGAGCCATTGGCGGGAAAACGCATGGTAAAAATC
ACCGAAAGAAAAACCAAACGGGACTGGGCTTGTTTTTTGGAAGAGATTGCAGATCAATACAAAGGAGCGAAAAAAATAACGCTGGTGATGGATAATCTGA
ACACACACGTCCCCGGATCGCTCTATGAAACTTTTCCGCCGGATAAGGCAAAGGCAATATTGGACAGATTCGAATTTGTGTACACCCCGAAGCATGGGAG
CTGGTTGAATATGGCAGAGATTGAGTTAAATGTACTCACCGGGCAGTGTTTAAATAGGAGAATCGATGACATTGCGGTTGTAAGGAGAGAGGTACTGGCA
TGGCAGAAATTCAGAAACAACAAAAGTGCGAAAGTTAATTGGCAATTCACAACCGAGGATGCACGGATAAAGTTATCTCGCCTTTATCCGACACTTGAAA
GTTGACACGACA
AAGATGAGCGCGAAACTCTTGGCGCACTTACTTCCAAAGGTAAACATAAATCACAGAAAATCCTTAACGCACTGATTTTATTGGGGTGCGATGAGGGGGA
GTATCAAAAGAAACGTTCGACCAATGAAGAAATGGCTCGCATCCTGAACATAAGTATGAAAAAGATCGATCGTGTGAAGAAACGATTTGTCATGGACGGT
ATCGAGGTTGCCCTTAACGGGAGAAAGGGAAGCAGTATCTACGCTAAAAAAGTGGATGGCGATTTTGAAGCCCATTTGGTTGCAGTGAGTTGCAGTGAGC
CACCCGAAGGCTTTGCGAGATGGTCTCTGAGGTTGTTAGCCGACAAGGTTGTTGAGCTGGATTATATTGATAGCATTTCCCATGAAACGGTACGCCGTAT
CCTAAAAAAAACGAAATCAAACCCTGGCAACGAAAAGGATGGGTAATTCCTCCAGAGCAAAACGGTAACTTTGTTGCGAATATGGAAATGGTGTTGGATG
TTTACAAGCGTCCGTTTGATCCACTATATCCCGTTCTAGGCATGGACGAATCCCCAAAGCAGCTGATTGCAGAGACAAGAACGCCCATTCCTGCTTCGCC
CGGGCAGCCGACCAAACATGATTATGAATATAGGCGTTGCGGCATGTGCAATATTTTTCTCGCCTGTGAGCCATTGGCGGGAAAACGCATGGTAAAAATC
ACCGAAAGAAAAACCAAACGGGACTGGGCTTGTTTTTTGGAAGAGATTGCAGATCAATACAAAGGAGCGAAAAAAATAACGCTGGTGATGGATAATCTGA
ACACACACGTCCCCGGATCGCTCTATGAAACTTTTCCGCCGGATAAGGCAAAGGCAATATTGGACAGATTCGAATTTGTGTACACCCCGAAGCATGGGAG
CTGGTTGAATATGGCAGAGATTGAGTTAAATGTACTCACCGGGCAGTGTTTAAATAGGAGAATCGATGACATTGCGGTTGTAAGGAGAGAGGTACTGGCA
TGGCAGAAATTCAGAAACAACAAAAGTGCGAAAGTTAATTGGCAATTCACAACCGAGGATGCACGGATAAAGTTATCTCGCCTTTATCCGACACTTGAAA
GTTGACACGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 40 | 546 | + | No |
Description : First part of the transposase
ORF sequence :
MFFIINLGGLNMKKYIVALAKDERETLGALTSKGKHKSQKILNALILLGCDEGEYQKKRSTNEEMARILNISMKKIDRVKKRFVMDGIEVALNGRKGSSI
YAKKVDGDFEAHLVAVSCSEPPEGFARWSLRLLADKVVELDYIDSISHETVRRILKKTKSNPGNEKDG
YAKKVDGDFEAHLVAVSCSEPPEGFARWSLRLLADKVVELDYIDSISHETVRRILKKTKSNPGNEKDG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 624 bp | 207 aa | 582 | 1205 | + | No |
Description : Second part of the transposase
ORF sequence :
MEMVLDVYKRPFDPLYPVLGMDESPKQLIAETRTPIPASPGQPTKHDYEYRRCGMCNIFLACEPLAGKRMVKITERKTKRDWACFLEEIADQYKGAKKIT
LVMDNLNTHVPGSLYETFPPDKAKAILDRFEFVYTPKHGSWLNMAEIELNVLTGQCLNRRIDDIAVVRREVLAWQKFRNNKSAKVNWQFTTEDARIKLSR
LYPTLES
LVMDNLNTHVPGSLYETFPPDKAKAILDRFEFVYTPKHGSWLNMAEIELNVLTGQCLNRRIDDIAVVRREVLAWQKFRNNKSAKVNWQFTTEDARIKLSR
LYPTLES
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 40 | 1205 | + | Yes |
Chemistry : DDE
ORF sequence :
MFFIINLGGLNMKKYIVALAKDERETLGALTSKGKHKSQKILNALILLGCDEGEYQKKRSTNEEMARILNISMKKIDRVKKRFVMDGIEVALNGRKGSSI
YAKKVDGDFEAHLVAVSCSEPPEGFARWSLRLLADKVVELDYIDSISHETVRRILKKNEIKPWQRKGWVIPPEQNGNFVANMEMVLDVYKRPFDPLYPVL
GMDESPKQLIAETRTPIPASPGQPTKHDYEYRRCGMCNIFLACEPLAGKRMVKITERKTKRDWACFLEEIADQYKGAKKITLVMDNLNTHVPGSLYETFP
PDKAKAILDRFEFVYTPKHGSWLNMAEIELNVLTGQCLNRRIDDIAVVRREVLAWQKFRNNKSAKVNWQFTTEDARIKLSRLYPTLES
YAKKVDGDFEAHLVAVSCSEPPEGFARWSLRLLADKVVELDYIDSISHETVRRILKKNEIKPWQRKGWVIPPEQNGNFVANMEMVLDVYKRPFDPLYPVL
GMDESPKQLIAETRTPIPASPGQPTKHDYEYRRCGMCNIFLACEPLAGKRMVKITERKTKRDWACFLEEIADQYKGAKKITLVMDNLNTHVPGSLYETFP
PDKAKAILDRFEFVYTPKHGSWLNMAEIELNVLTGQCLNRRIDDIAVVRREVLAWQKFRNNKSAKVNWQFTTEDARIKLSRLYPTLES
Blast result :
Comments
ISArch4 is 63% (fusion ORF )aa similar to ISMth1.
The ORF3 is a in silico fusion ORF.
The ORF3 is a in silico fusion ORF.
References
1] Hallam,S.J., Putnam,N., Preston,C.M., Detter,J.C., Rokhsar,D., Richardson,P.M. and DeLong,E.F. (2004) Science 305 (5689), 1457-1462