ISRtr2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY751757 | ND | Rhizobium tropici | Rhizobium tropici CFN299 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 26
IRL : TGAACCGCGCCGGGTTTGCCGGAGGCTCCAACTCGTGAGAAAGTGGAGCC
IRR : TGAACCGCGCCGGGTTTGCCGGAGGCCGTTTGGTTTAAGTTATGCGGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGAACCGCGCCGGGTTTGCCGGAGGCTCCAACTCGTGAGAAAGTGGAGCCGATATGAGCAAGACGACCAACAAATTTTCACCTGAAGTTCGTGACCGAGC
CATCCGCATGGTTTTGGACCATGAGGCCGAGCATCCATCCCGGTGGGCGGCAGTTTCATCCATAGCCGCCAAGATTGGCTGCTCGGCACATACGCTGCAT
GAGTGGGTGAAGAAAGCCGATGTTGACAGCGGCAAACGAGCAGGCCTTCCAAGCGATGTCGCCGAGAAGATGAAGGCCCTTGAGCGGGAGAACCGCGAGC
TTCGACAGGCCAATGAGATTTTGCGTAAGGCGTCGGCTTATTTTGCCCAGGCGGAGCTCGACCGCCCACTGAAGCGATGATTTCCTTCATCGACGAACAC
CGCTCAAAGTTCGGGGTCGAGCCGATCTGCAGGCTGCTGCCGATTGCCCCGTCCACTTACTATGACGCCATCGCCAAGCGCACGGACGTGGACCGCCTGT
CGGCCCGCGCTCGTCGCGACGCGGCCATGAAGGTCGAGATACGCCGAGTGTTCAATGAGAATTTCCAAGTCTACGGCGTGCGGAAAGTATGGCGGCAGTT
GCAGCGGGAAGGCTTCGACATAGCTCGATGCACTGTTTCTCGGCTTATGAGAATGATGGGTCTTCAGGGCATTATCCGTGGAAAGCCCGTCAAAACGACC
GTGTCTGACAAGGCCGCTCTATGTCCGCTCGACCGCGTGAACCGCCAGTTCTGCGCTCCCGCGCCGAACATGTTGTGGCTTTCCGATTTCACGTATGTAG
CCACTTGGCAAGGCTTCGTTTACGTCGCTTTCGTGATCGACGCGTTCGCCCGCCGCATCGTCGGCTGGCGGGCGAGCCGAACCGCTCATGCGGGTTTCGT
CCTCGATGCCCTGGACCAGGCACTCCATGACCGGCGGCCAGTCCATCGCGGTGGGCTCATCCACCACTCCGACAGGGGTGTTCAATACGTATCGATTAAG
TATTCCGAACGGCTGGCGGAGGCAGGCATCGAGCCTTCCGTTGGAAGCGTCGGCGACAGTTACGACAACGCTCTCGCCGAAACGATCAACGGCCTCTACA
AGGCCGAGGTCATCCATCGGCGGGGACCATGGCGCAATTTCGAAGCCGTGGAGTTCGCCACACTCGAATGGGTGGATTGGTTCAACAACCGGCGTCTTCT
GGAGCCCATAGGAAACATTCCGCCTGCCGAAGCCGAAGAACGTTACTATGCCATGCTGGACGAGCCAGCCATGGCCGCATAACTTAAACCAAACGGCCTC
CGGCAAACCCGGCGCGGTTCA
CATCCGCATGGTTTTGGACCATGAGGCCGAGCATCCATCCCGGTGGGCGGCAGTTTCATCCATAGCCGCCAAGATTGGCTGCTCGGCACATACGCTGCAT
GAGTGGGTGAAGAAAGCCGATGTTGACAGCGGCAAACGAGCAGGCCTTCCAAGCGATGTCGCCGAGAAGATGAAGGCCCTTGAGCGGGAGAACCGCGAGC
TTCGACAGGCCAATGAGATTTTGCGTAAGGCGTCGGCTTATTTTGCCCAGGCGGAGCTCGACCGCCCACTGAAGCGATGATTTCCTTCATCGACGAACAC
CGCTCAAAGTTCGGGGTCGAGCCGATCTGCAGGCTGCTGCCGATTGCCCCGTCCACTTACTATGACGCCATCGCCAAGCGCACGGACGTGGACCGCCTGT
CGGCCCGCGCTCGTCGCGACGCGGCCATGAAGGTCGAGATACGCCGAGTGTTCAATGAGAATTTCCAAGTCTACGGCGTGCGGAAAGTATGGCGGCAGTT
GCAGCGGGAAGGCTTCGACATAGCTCGATGCACTGTTTCTCGGCTTATGAGAATGATGGGTCTTCAGGGCATTATCCGTGGAAAGCCCGTCAAAACGACC
GTGTCTGACAAGGCCGCTCTATGTCCGCTCGACCGCGTGAACCGCCAGTTCTGCGCTCCCGCGCCGAACATGTTGTGGCTTTCCGATTTCACGTATGTAG
CCACTTGGCAAGGCTTCGTTTACGTCGCTTTCGTGATCGACGCGTTCGCCCGCCGCATCGTCGGCTGGCGGGCGAGCCGAACCGCTCATGCGGGTTTCGT
CCTCGATGCCCTGGACCAGGCACTCCATGACCGGCGGCCAGTCCATCGCGGTGGGCTCATCCACCACTCCGACAGGGGTGTTCAATACGTATCGATTAAG
TATTCCGAACGGCTGGCGGAGGCAGGCATCGAGCCTTCCGTTGGAAGCGTCGGCGACAGTTACGACAACGCTCTCGCCGAAACGATCAACGGCCTCTACA
AGGCCGAGGTCATCCATCGGCGGGGACCATGGCGCAATTTCGAAGCCGTGGAGTTCGCCACACTCGAATGGGTGGATTGGTTCAACAACCGGCGTCTTCT
GGAGCCCATAGGAAACATTCCGCCTGCCGAAGCCGAAGAACGTTACTATGCCATGCTGGACGAGCCAGCCATGGCCGCATAACTTAAACCAAACGGCCTC
CGGCAAACCCGGCGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 54 | 380 | + | No |
Description : First part of the transposase
ORF sequence :
MSKTTNKFSPEVRDRAIRMVLDHEAEHPSRWAAVSSIAAKIGCSAHTLHEWVKKADVDSGKRAGLPSDVAEKMKALERENRELRQANEILRKASAYFAQA
ELDRPLKR
ELDRPLKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 377 | 1282 | + | No |
Description : Second part of the transposase
ORF sequence :
MISFIDEHRSKFGVEPICRLLPIAPSTYYDAIAKRTDVDRLSARARRDAAMKVEIRRVFNENFQVYGVRKVWRQLQREGFDIARCTVSRLMRMMGLQGII
RGKPVKTTVSDKAALCPLDRVNRQFCAPAPNMLWLSDFTYVATWQGFVYVAFVIDAFARRIVGWRASRTAHAGFVLDALDQALHDRRPVHRGGLIHHSDR
GVQYVSIKYSERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRGPWRNFEAVEFATLEWVDWFNNRRLLEPIGNIPPAEAEERYYAMLDEPAMA
A
RGKPVKTTVSDKAALCPLDRVNRQFCAPAPNMLWLSDFTYVATWQGFVYVAFVIDAFARRIVGWRASRTAHAGFVLDALDQALHDRRPVHRGGLIHHSDR
GVQYVSIKYSERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRGPWRNFEAVEFATLEWVDWFNNRRLLEPIGNIPPAEAEERYYAMLDEPAMA
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1229 bp | 409 aa | 54 | 1282 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKTTNKFSPEVRDRAIRMVLDHEAEHPSRWAAVSSIAAKIGCSAHTLHEWVKKADVDSGKRAGLPSDVAEKMKALERENRELRQANEILRKASAYFCPG
GARPPTEAMISFIDEHRSKFGVEPICRLLPIAPSTYYDAIAKRTDVDRLSARARRDAAMKVEIRRVFNENFQVYGVRKVWRQLQREGFDIARCTVSRLMR
MMGLQGIIRGKPVKTTVSDKAALCPLDRVNRQFCAPAPNMLWLSDFTYVATWQGFVYVAFVIDAFARRIVGWRASRTAHAGFVLDALDQALHDRRPVHRG
GLIHHSDRGVQYVSIKYSERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRGPWRNFEAVEFATLEWVDWFNNRRLLEPIGNIPPAEAEERYYA
MLDEPAMAA
GARPPTEAMISFIDEHRSKFGVEPICRLLPIAPSTYYDAIAKRTDVDRLSARARRDAAMKVEIRRVFNENFQVYGVRKVWRQLQREGFDIARCTVSRLMR
MMGLQGIIRGKPVKTTVSDKAALCPLDRVNRQFCAPAPNMLWLSDFTYVATWQGFVYVAFVIDAFARRIVGWRASRTAHAGFVLDALDQALHDRRPVHRG
GLIHHSDRGVQYVSIKYSERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRGPWRNFEAVEFATLEWVDWFNNRRLLEPIGNIPPAEAEERYYA
MLDEPAMAA
Blast result :
Comments
ISRtr2 is 90% (ORFA) and 93% (ORFB) aa similar to IS868. There is a frameshift motif TTTT (position 341-344).
References
1] Hernandez-Lucas,I., Xianwu,G., Gaytan,M.A., Ramirez-Trujillo,A., Perez-Rueda,E., Flores,M., Broughton,W., Martinez-Romero,E. and Mavingui,P. (2006) Direct submission.