ISMma17
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1075 bp
Ends
IR Length : 22/27
IRL : TAGTGACCCTTCTATATAATGCAACATTTATATAATTGAAGTAATTATAT
IRR : TAGTCACCATTTTAAATAATGCGACATCTTGTGCGTGAATACATGCTTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAAACTTGACAACCAGA | TA | ATATGAGTTTCTAATT | 2 |
DNA sequence
TAGTGACCCTTCTATATAATGCAACATTTATATAATTGAAGTAATTATATTATTTTAATTATGAGTTTGGAAATGTTTACTACTTCTGATGTTGCCACGG
CTATCTGTAGTTCCTGTGACAAGAATGAGAAGTTAAAACTTCAAGCGTTGCTGTTAGTTAAAAATGGTTTGAGTCCAGCTAAAGTTGCAGAAGATTTCTC
AGTTCATCGTTCAACAGTTCATCGTTGGATGAAAAGAGCTGAAGAAGAAGGTTTGTCCAGCCTTAAATGTAGACCTGGTCGAGGAGTAAAGTCTTTTTTA
ACTGAAGAGCAACTTTCTGTGTTAAAGAAAGCATTATCAGAGCCAATACCAACAGATGATGGCTTTTCTCGTGGTTGGCAAACTAAGGATGCAATTCAAT
TTGTCAGAGAGAAATTTGGAATATCTTATTCCAAATCAAGAATGAGACAAATTATTAAAAATTTAGGATTTAGTAGAATTACATGTAGACCTCAATCAAA
AAGACGAAATCAGGCATTAACAAACGAATTTATTGCTGAAATCAAAAAAAAGACTCTTAAATCCTGACTATTTATTTGTCACTCAGGATGAGAGTAGTTT
TTATTTAGACTCAAACAGATCAAAATGTTGGGCAATAAAAGGCTCTAAGCCTATTAAATTTATAAGTGGTTCAAAAACAAAAATCAATATTGGAGGATTC
TACACTGAAAACAGAGACTTTTATTGGTATGATTTAGGGATTAAAAAGAATACAGACTCTTTCTTAAAATCATTAATAAAGCTTAAAAAAGACATAGGAA
GAAAAATATTTCTTCTACTGGACAGAGCAACATGGCATAAATCGAAAAAAGCAAGAGAATTCTTTCAAAATAACAAATATTGGTTGCAAATATTACTTTT
CCCTCCAGCTACTCCAGATAGGAACCCAACAGAATATTGTTGGAAAACGACAAGAGAAGAGTTAACGTCAATAAAATCATTCAAGAATATTAAAGTATTA
AAGGAAGAACTAGATGAGTTTTGGGAGAAGCATGTATTCACGCACAAGATGTCGCATTATTTAAAATGGTGACTA
CTATCTGTAGTTCCTGTGACAAGAATGAGAAGTTAAAACTTCAAGCGTTGCTGTTAGTTAAAAATGGTTTGAGTCCAGCTAAAGTTGCAGAAGATTTCTC
AGTTCATCGTTCAACAGTTCATCGTTGGATGAAAAGAGCTGAAGAAGAAGGTTTGTCCAGCCTTAAATGTAGACCTGGTCGAGGAGTAAAGTCTTTTTTA
ACTGAAGAGCAACTTTCTGTGTTAAAGAAAGCATTATCAGAGCCAATACCAACAGATGATGGCTTTTCTCGTGGTTGGCAAACTAAGGATGCAATTCAAT
TTGTCAGAGAGAAATTTGGAATATCTTATTCCAAATCAAGAATGAGACAAATTATTAAAAATTTAGGATTTAGTAGAATTACATGTAGACCTCAATCAAA
AAGACGAAATCAGGCATTAACAAACGAATTTATTGCTGAAATCAAAAAAAAGACTCTTAAATCCTGACTATTTATTTGTCACTCAGGATGAGAGTAGTTT
TTATTTAGACTCAAACAGATCAAAATGTTGGGCAATAAAAGGCTCTAAGCCTATTAAATTTATAAGTGGTTCAAAAACAAAAATCAATATTGGAGGATTC
TACACTGAAAACAGAGACTTTTATTGGTATGATTTAGGGATTAAAAAGAATACAGACTCTTTCTTAAAATCATTAATAAAGCTTAAAAAAGACATAGGAA
GAAAAATATTTCTTCTACTGGACAGAGCAACATGGCATAAATCGAAAAAAGCAAGAGAATTCTTTCAAAATAACAAATATTGGTTGCAAATATTACTTTT
CCCTCCAGCTACTCCAGATAGGAACCCAACAGAATATTGTTGGAAAACGACAAGAGAAGAGTTAACGTCAATAAAATCATTCAAGAATATTAAAGTATTA
AAGGAAGAACTAGATGAGTTTTGGGAGAAGCATGTATTCACGCACAAGATGTCGCATTATTTAAAATGGTGACTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 61 | 567 | + | No |
Description : First part of the transposase
ORF sequence :
MSLEMFTTSDVATAICSSCDKNEKLKLQALLLVKNGLSPAKVAEDFSVHRSTVHRWMKRAEEEGLSSLKCRPGRGVKSFLTEEQLSVLKKALSEPIPTDD
GFSRGWQTKDAIQFVREKFGISYSKSRMRQIIKNLGFSRITCRPQSKRRNQALTNEFIAEIKKKTLKS
GFSRGWQTKDAIQFVREKFGISYSKSRMRQIIKNLGFSRITCRPQSKRRNQALTNEFIAEIKKKTLKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 540 bp | 179 aa | 533 | 1072 | + | No |
Description : Second part of the transposase
ORF sequence :
MLKSKKRLLNPDYLFVTQDESSFYLDSNRSKCWAIKGSKPIKFISGSKTKINIGGFYTENRDFYWYDLGIKKNTDSFLKSLIKLKKDIGRKIFLLLDRAT
WHKSKKAREFFQNNKYWLQILLFPPATPDRNPTEYCWKTTREELTSIKSFKNIKVLKEELDEFWEKHVFTHKMSHYLKW
WHKSKKAREFFQNNKYWLQILLFPPATPDRNPTEYCWKTTREELTSIKSFKNIKVLKEELDEFWEKHVFTHKMSHYLKW
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1012 bp | 337 aa | 61 | 1072 | + | Yes |
Chemistry : DDE
ORF sequence :
MSLEMFTTSDVATAICSSCDKNEKLKLQALLLVKNGLSPAKVAEDFSVHRSTVHRWMKRAEEEGLSSLKCRPGRGVKSFLTEEQLSVLKKALSEPIPTDD
GFSRGWQTKDAIQFVREKFGISYSKSRMRQIIKNLGFSRITCRPQSKRRNQALTNEFIAEIKKKRLLNPDYLFVTQDESSFYLDSNRSKCWAIKGSKPIK
FISGSKTKINIGGFYTENRDFYWYDLGIKKNTDSFLKSLIKLKKDIGRKIFLLLDRATWHKSKKAREFFQNNKYWLQILLFPPATPDRNPTEYCWKTTRE
ELTSIKSFKNIKVLKEELDEFWEKHVFTHKMSHYLKW
GFSRGWQTKDAIQFVREKFGISYSKSRMRQIIKNLGFSRITCRPQSKRRNQALTNEFIAEIKKKRLLNPDYLFVTQDESSFYLDSNRSKCWAIKGSKPIK
FISGSKTKINIGGFYTENRDFYWYDLGIKKNTDSFLKSLIKLKKDIGRKIFLLLDRATWHKSKKAREFFQNNKYWLQILLFPPATPDRNPTEYCWKTTRE
ELTSIKSFKNIKVLKEELDEFWEKHVFTHKMSHYLKW
Blast result :
Comments
ISMma17 is 47% aa similar to ISC1048. ORF3 is an in silico fusion.
References
1]Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baeumer,S., Jacobi,C., Brueggemann,H., Lienard,T., Christmann,A., Boemecke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.-P., Gunsalus,R.P., Fritz,H.-J. and Gottschalk,G. (2002)J. Mol. Microbiol. Biotechnol.