ISMma15
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1560 bp
Ends
IR Length : 17/19
IRL : GTAACTATTCAGGTAGAGCCTTTGAAAGGCTACAATTTTTCCTCTTTTCG
IRR : GTAACTATTCAGGGTATAGTTAACGGCGCTCTCTTGAGCGCCGCTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACAATAAAA | ACATTATT | TTACTTTTTT | 8 |
| AACAAGGATA | AATTGAGT | ATTAAATGAG | 8 |
DNA sequence
GTAACTATTCAGGTAGAGCCTTTGAAAGGCTACAATTTTTCCTCTTTTCGAGGAAAAATTGCATATAAATTGAATCCTATTTCCTTTCGTTATTATGCTT
ACACGTGAAGAGATTCTTATCATTTATGACGCTGGTCCTGAAGCTGTAATTTCTGTAATCCAGAGGCTTGAGACTATCATAGAAGAACAATCTATTCGTA
TTGCTGAACTCGAAGAACGTGTAAAGGTATTAGAATCTCGTTTAAACCAAAACAGTCGTAACAGCAGTCGCCCTCCTTCTACTGATTTTTTTATCAAGGA
GAAACCTAACCCCAAGAGTCTCCGTAAAAAGAGTGGCAAAAAACCTGGAGGTCAAGATGGTCATCCAGGAACAACTCTTGAAATGGTTGATCATCCTGAG
TAGGTAATAGAACATTCTTTGAGTTGCTGCAAAGAATGTGGCCATACTCTTGAGAATGTTGAAGTTGAAGCCTATGAGAAAAGACAGGTCTTTGATATTC
CTCCTGTAAATCTGATTGTTACAGAACACAAAAGTCAGATAAAGACCTGCCCTTACTGTGGAAAAATAAATAAAGCTGTTTTTCCTGAATCAGTTAAATA
TCCAGTTCAGTATGGTCCAAATATTTTAGCTTCAGCTATTTACTGTAAAAATCACCATTTTATCCCCTATGAAAGAATTTCCGAATTTTTTGAGGACATA
ATGGGAATAAAAATCTGTCCTGCTACGATAATTAGAGCAGAAAGAGAATGTTTCCAGAATTTAGAGGAATTTGAAAACGTTATTAGAGAGAAGTTATTAG
CCTCGCCTGTAATCAATTTTGATGAAACTGGTATGAAGATTGAAGGAAAAAGACACTGGCTTCATGTAGATTCTAATGAGAAATACACCTGTTATTTACC
TCACTCAAAAAGAGGAGCAGAAGCAATAGACGCTATGGGTATTCTTCCGGAGTTTAAGGGAGTAGCAGTTCACGACGGATGGAAACCTTACAACGTTTAT
GACTGTGATCATGCTCTCTGTAATGCTCATTTACAGAGAGAACTTACTGGAATTGAAGAGAACTATAAACAGCAGTGGGCTAAAGAAATGAATGAATTGC
TCACTGAGATGAAAAAGTATACCGACGAATGCAAGGATCAAGTCAAAGAACTGGATTTTGAGCAAATTAAAGCATTGGAAGAAAGGTTCGATGCTATCAT
AATTAAAGGAATTGAAGAAAATCCACAATCTCTAAATCCTGAAAAGAAAGGAAAACGTGGAAAAAATCCAAAAACAAAAGCAAGGAATCTGCTAGATAGG
TTTATAGAACACAAAGAAAATATTCTGAGATTCCTGACAGATTTGAAAGTTCCGTTTGAGAATAATCAAGCAGAAAGAGATATCAGGATGATGAAATTAC
AGCAGAAAATATCAGGAACTTTCAGAACTATACAAGGAGCGGAAGCTTTCTGCAGAATTAGAGCTTACATCTCTACAATTAGAAAGAACGGATTACCTGT
TTTGGAGGGTATTATAGCGGCGCTCAAGAGAGCGCCGTTAACTATACCCTGAATAGTTAC
ACACGTGAAGAGATTCTTATCATTTATGACGCTGGTCCTGAAGCTGTAATTTCTGTAATCCAGAGGCTTGAGACTATCATAGAAGAACAATCTATTCGTA
TTGCTGAACTCGAAGAACGTGTAAAGGTATTAGAATCTCGTTTAAACCAAAACAGTCGTAACAGCAGTCGCCCTCCTTCTACTGATTTTTTTATCAAGGA
GAAACCTAACCCCAAGAGTCTCCGTAAAAAGAGTGGCAAAAAACCTGGAGGTCAAGATGGTCATCCAGGAACAACTCTTGAAATGGTTGATCATCCTGAG
TAGGTAATAGAACATTCTTTGAGTTGCTGCAAAGAATGTGGCCATACTCTTGAGAATGTTGAAGTTGAAGCCTATGAGAAAAGACAGGTCTTTGATATTC
CTCCTGTAAATCTGATTGTTACAGAACACAAAAGTCAGATAAAGACCTGCCCTTACTGTGGAAAAATAAATAAAGCTGTTTTTCCTGAATCAGTTAAATA
TCCAGTTCAGTATGGTCCAAATATTTTAGCTTCAGCTATTTACTGTAAAAATCACCATTTTATCCCCTATGAAAGAATTTCCGAATTTTTTGAGGACATA
ATGGGAATAAAAATCTGTCCTGCTACGATAATTAGAGCAGAAAGAGAATGTTTCCAGAATTTAGAGGAATTTGAAAACGTTATTAGAGAGAAGTTATTAG
CCTCGCCTGTAATCAATTTTGATGAAACTGGTATGAAGATTGAAGGAAAAAGACACTGGCTTCATGTAGATTCTAATGAGAAATACACCTGTTATTTACC
TCACTCAAAAAGAGGAGCAGAAGCAATAGACGCTATGGGTATTCTTCCGGAGTTTAAGGGAGTAGCAGTTCACGACGGATGGAAACCTTACAACGTTTAT
GACTGTGATCATGCTCTCTGTAATGCTCATTTACAGAGAGAACTTACTGGAATTGAAGAGAACTATAAACAGCAGTGGGCTAAAGAAATGAATGAATTGC
TCACTGAGATGAAAAAGTATACCGACGAATGCAAGGATCAAGTCAAAGAACTGGATTTTGAGCAAATTAAAGCATTGGAAGAAAGGTTCGATGCTATCAT
AATTAAAGGAATTGAAGAAAATCCACAATCTCTAAATCCTGAAAAGAAAGGAAAACGTGGAAAAAATCCAAAAACAAAAGCAAGGAATCTGCTAGATAGG
TTTATAGAACACAAAGAAAATATTCTGAGATTCCTGACAGATTTGAAAGTTCCGTTTGAGAATAATCAAGCAGAAAGAGATATCAGGATGATGAAATTAC
AGCAGAAAATATCAGGAACTTTCAGAACTATACAAGGAGCGGAAGCTTTCTGCAGAATTAGAGCTTACATCTCTACAATTAGAAAGAACGGATTACCTGT
TTTGGAGGGTATTATAGCGGCGCTCAAGAGAGCGCCGTTAACTATACCCTGAATAGTTAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1458 bp | 485 aa | 95 | 1552 | + | No |
Description :
ORF sequence :
MLTREEILIIYDAGPEAVISVIQRLETIIEEQSIRIAELEERVKVLESRLNQNSRNSSRPPSTDFFIKEKPNPKSLRKKSGKKPGGQDGHPGTTLEMVDH
PE*VIEHSLSCCKECGHTLENVEVEAYEKRQVFDIPPVNLIVTEHKSQIKTCPYCGKINKAVFPESVKYPVQYGPNILASAIYCKNHHFIPYERISEFFE
DIMGIKICPATIIRAERECFQNLEEFENVIREKLLASPVINFDETGMKIEGKRHWLHVDSNEKYTCYLPHSKRGAEAIDAMGILPEFKGVAVHDGWKPYN
VYDCDHALCNAHLQRELTGIEENYKQQWAKEMNELLTEMKKYTDECKDQVKELDFEQIKALEERFDAIIIKGIEENPQSLNPEKKGKRGKNPKTKARNLL
DRFIEHKENILRFLTDLKVPFENNQAERDIRMMKLQQKISGTFRTIQGAEAFCRIRAYISTIRKNGLPVLEGIIAALKRAPLTIP
PE*VIEHSLSCCKECGHTLENVEVEAYEKRQVFDIPPVNLIVTEHKSQIKTCPYCGKINKAVFPESVKYPVQYGPNILASAIYCKNHHFIPYERISEFFE
DIMGIKICPATIIRAERECFQNLEEFENVIREKLLASPVINFDETGMKIEGKRHWLHVDSNEKYTCYLPHSKRGAEAIDAMGILPEFKGVAVHDGWKPYN
VYDCDHALCNAHLQRELTGIEENYKQQWAKEMNELLTEMKKYTDECKDQVKELDFEQIKALEERFDAIIIKGIEENPQSLNPEKKGKRGKNPKTKARNLL
DRFIEHKENILRFLTDLKVPFENNQAERDIRMMKLQQKISGTFRTIQGAEAFCRIRAYISTIRKNGLPVLEGIIAALKRAPLTIP
Blast result :
Comments
ISMma15 is 94% aa similar to ISMma13.
There is a Stop codon at position 103 of the transposase.
There is a Stop codon at position 103 of the transposase.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C., Bruggemann,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G. (2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461.