ISMhu3
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007796 | ND | Methanospirillum hungatei | Methanospirillum hungatei JF-1 |
DNA section
IS Length : 1727 bp
Ends
IR Length : 9/12
IRL : GTAAGTATTAAATACCATAGATATCTAATAGATATCTAATGAGAACTGTT
IRR : GTAACTACTCAACTTTAGGAAGTTGAACAATTGCCATTCTCGACAGGTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAAAATCAAGGGAATTTA | ATTTTATCTTGAGGACTCATTCA | 0 | |
| TCCAAAGCTGTCTTACATGA | AAAAAAGGATTATATCTTCTCTA | 0 |
DNA sequence
GTAAGTATTAAATACCATAGATATCTAATAGATATCTAATGAGAACTGTTCCATTCAATGTTGCTACAGAACTGAAGGTAAACAACATTTGTGGATTTTA
TAAACGAGAAGTCAAACCCTTTGGAACAAGTGCGAAAGTAGATTGTCCCAAGGAACATTTGGGTAAAACGGTTTATTTGGTTATTCTGGATAATGACGAA
TAATAGGACTTCCGAAATCAGTGATGAATCTATAGACGATTTACGGCGTAATATCCATCAATTAAAGAAAGAACTTAAAATTGAACGTAAACGCAGAATT
TTAGCAGAAAACCAAAAAAATGAACTGAAAAAGGAAAATAAAAAACTCAAAAAAGAAATTGCAAAAATATTGAGTTCTGCACCTTTTCTTTCAGCATCAA
GTAAAACAGCTAAAGCAGGAGGAGTTCCGAGTTCAAAGACCTTTTATCGCCGGAAGAGGCAGTGTGAAGAAAATAGGAAAAGTGGAGGTCAACCAGGGCA
TGAAGGAAATGGAAGGAAAAGACCCGCTTCAAATTCACCATCGATGAATATCCCCTTGGATTCATGTCCTGAATGTGGAACGCATTTGAATGAACCAGTC
AACGGGGCGGAACAAACACGCACCATTACGGATATTCCATTTCCAAGGCACATCGTTTACGAAATCCATTATCCGAGGTATTGGTGTTCAACTTGCAAGA
AATTAGTACGTGGAGAGTTGCCCTTACCGCCAAACCAGCAATTCGGACCTGCTGTAGCCTCTTGGATTGCTTATCATAGAATGTTAGGATTGACTATAAG
GAAAATCCAATCCAGCCTCTTAGAAACATACGATATTCAAATGAGTGAAGCTACCATTCTGAAACTGGAAAGGTGGGTTGCAGATACTCTCAAAGAAGAT
TACGAAAAACTCAAAGAAAAGATAATTCAGGAGAACAATATCAATGCAGATGAAACCAGTTTCAGAGTGAATGGAGAAAATGGATGGTTGTGGGTATTCA
CTTCGACAATTGGCTCATATTACAAAGTGGCATCAACTCGAGGACATTCGGTTCCCGAAGAAGTTTTAAAAGGATTTAATGGCGTTTTAGGAAGAGATGC
ATGGAAACCATATGATGTCATAATGTGTTCAGGGCATCAGTTAGATCTTCTTCATGTGAATCGATGGTTGGAAAGAGCAGAAATAAAGCACAAAATTGAA
CCGCGAAGTTTATTGACCTCTCAACCAGTCAAAATACTAAGAAAAGGCAGACCTCCAGAAAAATTTCTTGAATTTGTTGATGGAGTTCGTTCGATTCTAA
AAAAAGCGGTAGAATATACTGAAAATGATCCTCCACCATCTTTGGATGAACGGAAAAATGCACGTGATAAATTTCAGGCTGAGCTTACGGGCTTGCTTGA
ACGAAAATGGGTAGATCCAGATGTTATTCGTATTTTCAAAGAACTTCGAAAACGGCAAGATATGTTACTTACTTTCATGGTCCATGAGGATGTTCCAGGG
CATAATAATGATGCGGAACGTGCCATACGTCAGGGGGTACTTCATCGAAAAATTAGTGGGGGGAGAAGAACATGGCTCGGAGCTGAAGTATTTGGAGTCT
TGCTTAGTATTTACGAAACTTCGAAGAAACAAAAGGAGAATTTCATGCAATTGGTGGGAAGAAAGTTAGGATTTGAAGCACCTGTCGAGAATGGCAATTG
TTCAACTTCCTAAAGTTGAGTAGTTAC
TAAACGAGAAGTCAAACCCTTTGGAACAAGTGCGAAAGTAGATTGTCCCAAGGAACATTTGGGTAAAACGGTTTATTTGGTTATTCTGGATAATGACGAA
TAATAGGACTTCCGAAATCAGTGATGAATCTATAGACGATTTACGGCGTAATATCCATCAATTAAAGAAAGAACTTAAAATTGAACGTAAACGCAGAATT
TTAGCAGAAAACCAAAAAAATGAACTGAAAAAGGAAAATAAAAAACTCAAAAAAGAAATTGCAAAAATATTGAGTTCTGCACCTTTTCTTTCAGCATCAA
GTAAAACAGCTAAAGCAGGAGGAGTTCCGAGTTCAAAGACCTTTTATCGCCGGAAGAGGCAGTGTGAAGAAAATAGGAAAAGTGGAGGTCAACCAGGGCA
TGAAGGAAATGGAAGGAAAAGACCCGCTTCAAATTCACCATCGATGAATATCCCCTTGGATTCATGTCCTGAATGTGGAACGCATTTGAATGAACCAGTC
AACGGGGCGGAACAAACACGCACCATTACGGATATTCCATTTCCAAGGCACATCGTTTACGAAATCCATTATCCGAGGTATTGGTGTTCAACTTGCAAGA
AATTAGTACGTGGAGAGTTGCCCTTACCGCCAAACCAGCAATTCGGACCTGCTGTAGCCTCTTGGATTGCTTATCATAGAATGTTAGGATTGACTATAAG
GAAAATCCAATCCAGCCTCTTAGAAACATACGATATTCAAATGAGTGAAGCTACCATTCTGAAACTGGAAAGGTGGGTTGCAGATACTCTCAAAGAAGAT
TACGAAAAACTCAAAGAAAAGATAATTCAGGAGAACAATATCAATGCAGATGAAACCAGTTTCAGAGTGAATGGAGAAAATGGATGGTTGTGGGTATTCA
CTTCGACAATTGGCTCATATTACAAAGTGGCATCAACTCGAGGACATTCGGTTCCCGAAGAAGTTTTAAAAGGATTTAATGGCGTTTTAGGAAGAGATGC
ATGGAAACCATATGATGTCATAATGTGTTCAGGGCATCAGTTAGATCTTCTTCATGTGAATCGATGGTTGGAAAGAGCAGAAATAAAGCACAAAATTGAA
CCGCGAAGTTTATTGACCTCTCAACCAGTCAAAATACTAAGAAAAGGCAGACCTCCAGAAAAATTTCTTGAATTTGTTGATGGAGTTCGTTCGATTCTAA
AAAAAGCGGTAGAATATACTGAAAATGATCCTCCACCATCTTTGGATGAACGGAAAAATGCACGTGATAAATTTCAGGCTGAGCTTACGGGCTTGCTTGA
ACGAAAATGGGTAGATCCAGATGTTATTCGTATTTTCAAAGAACTTCGAAAACGGCAAGATATGTTACTTACTTTCATGGTCCATGAGGATGTTCCAGGG
CATAATAATGATGCGGAACGTGCCATACGTCAGGGGGTACTTCATCGAAAAATTAGTGGGGGGAGAAGAACATGGCTCGGAGCTGAAGTATTTGGAGTCT
TGCTTAGTATTTACGAAACTTCGAAGAAACAAAAGGAGAATTTCATGCAATTGGTGGGAAGAAAGTTAGGATTTGAAGCACCTGTCGAGAATGGCAATTG
TTCAACTTCCTAAAGTTGAGTAGTTAC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 165 bp | 54 aa | 39 | 203 | + | No |
AG : IS66 TnpB
ORF sequence :
MRTVPFNVATELKVNNICGFYKREVKPFGTSAKVDCPKEHLGKTVYLVILDNDE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1521 bp | 506 aa | 193 | 1713 | + | No |
Chemistry : DDE
ORF sequence :
MTNNRTSEISDESIDDLRRNIHQLKKELKIERKRRILAENQKNELKKENKKLKKEIAKILSSAPFLSASSKTAKAGGVPSSKTFYRRKRQCEENRKSGGQ
PGHEGNGRKRPASNSPSMNIPLDSCPECGTHLNEPVNGAEQTRTITDIPFPRHIVYEIHYPRYWCSTCKKLVRGELPLPPNQQFGPAVASWIAYHRMLGL
TIRKIQSSLLETYDIQMSEATILKLERWVADTLKEDYEKLKEKIIQENNINADETSFRVNGENGWLWVFTSTIGSYYKVASTRGHSVPEEVLKGFNGVLG
RDAWKPYDVIMCSGHQLDLLHVNRWLERAEIKHKIEPRSLLTSQPVKILRKGRPPEKFLEFVDGVRSILKKAVEYTENDPPPSLDERKNARDKFQAELTG
LLERKWVDPDVIRIFKELRKRQDMLLTFMVHEDVPGHNNDAERAIRQGVLHRKISGGRRTWLGAEVFGVLLSIYETSKKQKENFMQLVGRKLGFEAPVEN
GNCSTS
PGHEGNGRKRPASNSPSMNIPLDSCPECGTHLNEPVNGAEQTRTITDIPFPRHIVYEIHYPRYWCSTCKKLVRGELPLPPNQQFGPAVASWIAYHRMLGL
TIRKIQSSLLETYDIQMSEATILKLERWVADTLKEDYEKLKEKIIQENNINADETSFRVNGENGWLWVFTSTIGSYYKVASTRGHSVPEEVLKGFNGVLG
RDAWKPYDVIMCSGHQLDLLHVNRWLERAEIKHKIEPRSLLTSQPVKILRKGRPPEKFLEFVDGVRSILKKAVEYTENDPPPSLDERKNARDKFQAELTG
LLERKWVDPDVIRIFKELRKRQDMLLTFMVHEDVPGHNNDAERAIRQGVLHRKISGGRRTWLGAEVFGVLLSIYETSKKQKENFMQLVGRKLGFEAPVEN
GNCSTS
Blast result :
Comments
ISMhu3 is 81% (orfA) and 79%(orfB) aa similar to ISArch7.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Kiss,H., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N., McInerney,M.J., Brockman,F., Culley,D., Ferry,J.G., Gunsalus,R.P., Morrison,M., Plugge,C., Scholten,J., Stams,A.J.M., Boone,D.R. and Richardson,P. (2006) Direct submission GenBank.