ISPfu4

  • Family IS607
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003413 ND Pyrococcus furiosus
Pyrococcus furiosus DSM 3638
DNA section
IS Length : 1961 bp

Ends


IR Length : 0

IRL : GCCTTTTTTCACTGTTCCTCTTAACCGAAAGCTTTAAATATTTTCGTGAA
IRR : GGGGTCTGTCCGTCGTGAGTTTTTATGAGTGAATGTGAGTAAATCTTCGT

Insertion site


Left flankDirect repeatRight flankDR Length
TCTACTAGAGCTGAAAAATGATATCCAATAAATCC0

DNA sequence

GCCTTTTTTCACTGTTCCTCTTAACCGAAAGCTTTAAATATTTTCGTGAATAATTGTGAGTGTATGGTAGTAAAAGAGAAACTCTACACGGTAAAGCAGG
CAAGTGAGATACTCGGCGTCCACCCAAAGACAATCCAAAAATGGGACAGAGAAGGGAAAATCAAAACCGTTAGAACACCCGGCGGGAGAAGAAGAATACC
AGAAAGCGAAATAAAAAGACTCCTCGGAATAAGCGAGGAAAAAGGCCTAATCATCGGCTACGCAAGGGTATCAAGCCACACACAAAAAGACTACTTAGAA
AGACAAGTCAAAGCAATAGAGCAATACGCAAAAGAACGTGGGTGGCAAGTCCAAATACTCACGGACATCGGCTCAGGATTGAACGAGAACAGGAAAAACT
ACCGCAAACTCCTCGAACTCGTGGCAAAGAGAGAAGTCTCAAAAGTCATCATCACCCATCCCGACAGGCTTACACGCTTTGGCTTCAAAACTCTCGAATT
CTTCTTCAAGGAGAACGGTGCAGAGATAATCATTATCACCGACAAAGAAAAATCCCCACGAGAAGAACTCATTGAAGACTTAACAACCATAATCTCACAC
TTTGCTGGGAAACTCTACGGAATGCGCTCCCACAAATACAAAAAGCTCAAAGAAGGCGTAAAAAAACTAATCGAGGAGGTCGAGAATGGGTAAAATTGTC
TTAACATACAGAATGCCCCACAACTGGAACATTAATCCCTTCCTCAAGGAATACCAGAGACTCCTCCAGAGGGCAATTGACGAAATATGGGATAACACGA
GTTGGAAGGAAAAGAAGGTTAAGCATAGATATTCTCTCGGAAACGAGGAATACCGGTACTACGAGACAATCCGCCTAATCCCATATTTTCCACAATCAAA
CGATTTTAAGCGAGGGCTGAGGAATAAACTCCTCCGAGAATGGCCTTTTGCTAAGCACTACGTTGATTCTGCAATAAAGACTGCTTACTCAATCCTCAAA
AGCTGGAGGCAGAACTACCTCAAGGGGAGAAGAAAAAGAGTGAAACCAGTCGTTAAGAGGAAGTTCGTGAGGGTTAAAACAACACTAATGAAGGTTGAAG
GCTCGAAAATCAGAATAACAATTAAACCGAGGGAAGAATACCTTGAACTGGACTTCTCAAGGGAGTGGTTTTATGAGAGGATTAAGGATTGGAAAGTTGG
CGAGTTAATAATCAGGGAGAGGGATATCTTATTAACCTTCTCAAAGGAGGTTGAGTTCTCTGGAAGAATCAAAATCGGCATTGACAGTAACTTAACGAGC
CTTGACGTTTATCACCCTGAAAAGGGCTGGATTAGAGTGGACTTGAGCGAACTGCATAGAATTTCCGAGACTTATGATAGAATTATTGATATGCTAAAAA
GCATTCAGCGGAAAGCTCCAAAGAGGATTGGTTTATTGCTTGAGAAATACTGGACTAGGAGGAGGAACAGGATTGAGGATTACTTGAACAAACTCGCAGT
CCAGCTTTCGAGGGAGTTCCCCGATGCGATTTTCATCTTCGAGGGTTTGAACAAGTTTAAAATGCTCCAGAATGGTTCGAGAAAGTTTAACAGGAAGCTT
TCCCGTGCCACTTGGAAGAAGATTGTTGGAAAGCTTTCTTATCGTGTTCCTATCGAGTTTGTTAATCCTGCTTATACTTCCTCCACCTGCCCGATATGTG
GGAGTAAGTTAGAGTCCCGAAACGGGCTGGTGGAGTGTTTTAACTGTGGGTTTAAGGCGGATAGGCAGTTTGTTGGTGCTTTTAATATTTTGATGCGGGG
ACTTGGGGTCGCCCTGAGCGGGGTTGAGCGTGATGATTTGCCCCCCAATGAACCCAGAGGGGAGCTGAACGCGATGAGGCCCAAGTCCGTCGTGAGGGTT
GACTTGAATGGACGAAGATTTACTCACATTCACTCATAAAAACTCACGACGGACAGACCCC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
648 bp215 aa46693+No
ORF function : Transposase
Chemistry : Serine

ORF sequence :

MNNCECMVVKEKLYTVKQASEILGVHPKTIQKWDREGKIKTVRTPGGRRRIPESEIKRLLGISEEKGLIIGYARVSSHTQKDYLERQVKAIEQYAKERGW
QVQILTDIGSGLNENRKNYRKLLELVAKREVSKVIITHPDRLTRFGFKTLEFFFKENGAEIIIITDKEKSPREELIEDLTTIISHFAGKLYGMRSHKYKK
LKEGVKKLIEEVENG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1254 bp417 aa6861939+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MGKIVLTYRMPHNWNINPFLKEYQRLLQRAIDEIWDNTSWKEKKVKHRYSLGNEEYRYYETIRLIPYFPQSNDFKRGLRNKLLREWPFAKHYVDSAIKTA
YSILKSWRQNYLKGRRKRVKPVVKRKFVRVKTTLMKVEGSKIRITIKPREEYLELDFSREWFYERIKDWKVGELIIRERDILLTFSKEVEFSGRIKIGID
SNLTSLDVYHPEKGWIRVDLSELHRISETYDRIIDMLKSIQRKAPKRIGLLLEKYWTRRRNRIEDYLNKLAVQLSREFPDAIFIFEGLNKFKMLQNGSRK
FNRKLSRATWKKIVGKLSYRVPIEFVNPAYTSSTCPICGSKLESRNGLVECFNCGFKADRQFVGAFNILMRGLGVALSGVERDDLPPNEPRGELNAMRPK
SVVRVDLNGRRFTHIHS

 

Blast result :
Comments
ISPfu4 is 69% (ORF A) and 52% (ORF B) aa similar to IS1921.
References
1] Robb,F.T., Maeder,D.L., Brown,J.R., DiRuggiero,J., Stump,M.D., Yeh,R.K., Weiss,R.B. and Dunn,D.M. (2001) Meth. Enzymol. 330, 134-157