ISMac13
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003552 | ND | Methanosarcina acetivorans | Methanosarcina acetivorans C2A |
DNA section
IS Length : 1284 bp
Ends
IR Length : 19/21
IRL : TACATCGATTCCAAATTTATTATGTAAATTAACATATAATTAGTTTTATA
IRR : TACATCGATTCCTAATTAATTGCATATTTGTTTCATATTTCAATTCCACC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGATAAAATATATTTTGTATGAAA | TACTAG | TGCATATAAATTCTAATCAA | 6 |
DNA sequence
TACATCGATTCCAAATTTATTATGTAAATTAACATATAATTAGTTTTATATTTTTATGTATGGCTCGCATAAGTCGTCGTCCCAAATTATCACTTACACA
TGAAGAAATAAAATATTTGGAAAAACTAAGCCACTCTAGAAGTGAACCTGCAAGTCTTGTTCAAAGAGCTAAAATTGTGCTTTTAAGCTTTGAGGGAATA
AACGATACAGAAATCTCTCGGCAACTAAACGTTGCTTATAATACCGTAAGATCTCGAATACAAAGAGTTCTGGATTTTGGGGTCATAGAAGGCCTGAAGG
ATAAAACAGGAGCTGGAAGACCAAGGACAATTACAGATGAATCCCGTGTATGGCTGATCAAGGTTGCATGTATGAAACCAAAGGACTTTGGGTATCCGCA
TGAAATCTGGACACAGAGACTCTTAACTAAATATGTACAGGAAAACTGTATCAAGGAAGGATATCCGGAATTATCAAAGATCAGCCAGGGAACCATTTCA
AAAATCTTGAATGCAAGTAACATGTGAGTGCCTATCGGTCAAATGGGGGTTTAGAAAAACCACATAAAACAAGGTATTACGCCGCCAAAGTAGACCCGGA
TTTTGATGAAAAAGCAAGGAAGGTCCTGGGCACTTATAAGGAAGTTAAAAAACTAAAAAAAGAATTCAAAAAAAAGAAAAAGGTAGATAGAGTAATCCTT
TCATGTGATGAAAAAACAGGCATTCAAGCTATTGAAAATAAATACCCGGATGTGATGCCAACAGAAGGAAAATATCCTATGATGGCAAGAGATTATGAGT
ATATTAGACATGGTACTCTTTCATTATTTGCATGTATTGATTTGATTACCGGGAAAGTTTATCACAAAGTTTTTGAAAAAGGTAGAAGTCGTGAATTTAT
TGAGATTATAAAAGAAATAGTATCAGATTACCAGGATACAAAAATCGTAGCAACTCTTGATAACCACAAAATACACACATCAGAAGAAACTAATAAATAT
ATAAGTACCTTGGGAAAAGGGAAGTTAAAGTTCGTATTTACCCCAAAACATGCATCATGGTTGAATATTATTGAAAGTTTCATAAGCAAAATGAGTAGAG
GCTTTTTAAAGGCAATAAGAGTAAAATCAATAATGGAATTAAAGAACAGGATCGATCAATACATAAATTTACTCAATGAGGAACCTGTGGTATTTGTGTG
GAAATATAAAGTTGAAGGAGAAGACAGGATTCCCGGTGGAATTGAAATATGAAACAAATATGCAATTAATTAGGAATCGATGTA
TGAAGAAATAAAATATTTGGAAAAACTAAGCCACTCTAGAAGTGAACCTGCAAGTCTTGTTCAAAGAGCTAAAATTGTGCTTTTAAGCTTTGAGGGAATA
AACGATACAGAAATCTCTCGGCAACTAAACGTTGCTTATAATACCGTAAGATCTCGAATACAAAGAGTTCTGGATTTTGGGGTCATAGAAGGCCTGAAGG
ATAAAACAGGAGCTGGAAGACCAAGGACAATTACAGATGAATCCCGTGTATGGCTGATCAAGGTTGCATGTATGAAACCAAAGGACTTTGGGTATCCGCA
TGAAATCTGGACACAGAGACTCTTAACTAAATATGTACAGGAAAACTGTATCAAGGAAGGATATCCGGAATTATCAAAGATCAGCCAGGGAACCATTTCA
AAAATCTTGAATGCAAGTAACATGTGAGTGCCTATCGGTCAAATGGGGGTTTAGAAAAACCACATAAAACAAGGTATTACGCCGCCAAAGTAGACCCGGA
TTTTGATGAAAAAGCAAGGAAGGTCCTGGGCACTTATAAGGAAGTTAAAAAACTAAAAAAAGAATTCAAAAAAAAGAAAAAGGTAGATAGAGTAATCCTT
TCATGTGATGAAAAAACAGGCATTCAAGCTATTGAAAATAAATACCCGGATGTGATGCCAACAGAAGGAAAATATCCTATGATGGCAAGAGATTATGAGT
ATATTAGACATGGTACTCTTTCATTATTTGCATGTATTGATTTGATTACCGGGAAAGTTTATCACAAAGTTTTTGAAAAAGGTAGAAGTCGTGAATTTAT
TGAGATTATAAAAGAAATAGTATCAGATTACCAGGATACAAAAATCGTAGCAACTCTTGATAACCACAAAATACACACATCAGAAGAAACTAATAAATAT
ATAAGTACCTTGGGAAAAGGGAAGTTAAAGTTCGTATTTACCCCAAAACATGCATCATGGTTGAATATTATTGAAAGTTTCATAAGCAAAATGAGTAGAG
GCTTTTTAAAGGCAATAAGAGTAAAATCAATAATGGAATTAAAGAACAGGATCGATCAATACATAAATTTACTCAATGAGGAACCTGTGGTATTTGTGTG
GAAATATAAAGTTGAAGGAGAAGACAGGATTCCCGGTGGAATTGAAATATGAAACAAATATGCAATTAATTAGGAATCGATGTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1193 bp | 397 aa | 60 | 1252 | + | No |
Chemistry : DDE
ORF sequence :
MARISRRPKLSLTHEEIKYLEKLSHSRSEPASLVQRAKIVLLSFEGINDTEISRQLNVAYNTVRSRIQRVLDFGVIEGLKDKTGAGRPRTITDESRVWLI
KVACMKPKDFGYPHEIWTQRLLTKYVQENCIKEGYPELSKISQGTISKILNASNMVSAYRSNGGLEKPHKTRYYAAKVDPDFDEKARKVLGTYKEVKKLK
KEFKKKKKVDRVILSCDEKTGIQAIENKYPDVMPTEGKYPMMARDYEYIRHGTLSLFACIDLITGKVYHKVFEKGRSREFIEIIKEIVSDYQDTKIVATL
DNHKIHTSEETNKYISTLGKGKLKFVFTPKHASWLNIIESFISKMSRGFLKAIRVKSIMELKNRIDQYINLLNEEPVVFVWKYKVEGEDRIPGGIEI
KVACMKPKDFGYPHEIWTQRLLTKYVQENCIKEGYPELSKISQGTISKILNASNMVSAYRSNGGLEKPHKTRYYAAKVDPDFDEKARKVLGTYKEVKKLK
KEFKKKKKVDRVILSCDEKTGIQAIENKYPDVMPTEGKYPMMARDYEYIRHGTLSLFACIDLITGKVYHKVFEKGRSREFIEIIKEIVSDYQDTKIVATL
DNHKIHTSEETNKYISTLGKGKLKFVFTPKHASWLNIIESFISKMSRGFLKAIRVKSIMELKNRIDQYINLLNEEPVVFVWKYKVEGEDRIPGGIEI
Blast result :
Comments
ISMac13 is 49% aa similar to ISPsy1.
ISMac13 is disrupted by insertion of a 1695 bp sequence. A 37 bp at the end of this insertion is common to the IS : GTGAGTGCCTATCGGTCAAATGGGGGTTTAGAAAAA
There are 1 complete and 2 partial copies in Methanosarcina acetivorans C2A genome. There are 15 complete and 1 partial copies of 172 bp MITES in this genome.
ISMac13 is disrupted by insertion of a 1695 bp sequence. A 37 bp at the end of this insertion is common to the IS : GTGAGTGCCTATCGGTCAAATGGGGGTTTAGAAAAA
There are 1 complete and 2 partial copies in Methanosarcina acetivorans C2A genome. There are 15 complete and 1 partial copies of 172 bp MITES in this genome.
References
1] Galagan,J.E., Nusbaum,C., Roy,A., Endrizzi,M.G., Macdonald,P., FitzHugh,W., Calvo,S., Engels,R., Smirnov,S., Atnoor,D., Brown,A., Allen,N., Naylor,J., Stange-Thomann,N., DeArellano,K., Johnson,R., Linton,L., McEwan,P., McKernan,K., Talamas,J., Tirrell,A., Ye,W., Zimmer,A., Barber,R.D., Cann,I., Graham,D.E., Grahame,D.A., Guss,A.M., Hedderich,R., Ingram-Smith,C., Kuettner,H.C., Krzycki,J.A., Leigh,J.A., Li,W., Liu,J., Mukhopadhyay,B., Reeve,J.N., Smith,K., Springer,T.A., Umayam,L.A., White,O., White,R.H., Conway de Macario,E., Ferry,J.G., Jarrell,K.F., Jing,H., Macario,A.J., Paulsen,I., Pritchett,M., Sowers,K.R., Swanson,R.V., Zinder,S.H., Lander,E., Metcalf,W.W. and Birren,B. (2002) Genome Res. 12 (4), 532-542