ISH15
- Family IS6
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006396 | ND | Haloarcula marismortui | Halobacterium sp. Haloarcula marismortui plasmid pNG500 Haloarcula marismortui |
DNA section
IS Length : 697 bp
Ends
IR Length : 15/17
IRL : CGTAGTGTTCAGATAAGGATTGAAGAGTGAGGCACGGCGATTTTGAAGTG
IRR : CATCGTGTTCAGATAAGCTGATTCCATGCGAAGCTGAACGATCTGATCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATACCCGAAATACCAGCGGTTAC | GGGGTGAGT | CGTCCAAAGAGAAAGGGGGG | 9 |
| CGGTGTGACGGGCT | GTCGAGGT | GCTGGCGGTACTC | 8 |
| AAACTTTTGGAGA | AAACTAAT | GGGCCGTACTGTTT | 8 |
DNA sequence
CGTAGTGTTCAGATAAGGATTGAAGAGTGAGGCACGGCGATTTTGAAGTGTCTATGCCCAAAAACGCCGGCCTCGGCGGTAGTATCGACCAAATCGACTT
AGATTTTGTGGAGCGAGAAGCGACACCGCGACTGCTGATGAAGCTGAGTATTCAGCTGCATTTGGCTGGACTATCACTTTCGAATACTGTCTCGGTTCTT
GAAATATTCGGTGTCCAACGCGCTCGATCAACTGTCTATAATTGGGTTCACAAAGCAGATTTACAGCCCAAAGATGGACAGTCACCGGATCACGTTGCGG
TTGACGAAACTGTGATCCAACTCAACGGTGAGAGATATTGGCTGTACGCCGCTGTCGATCCACAAACGAACAAATTGCTTCATACGAAGCTTAGACCAAC
TACAACAAAGGTTCTCGCCCATGCATTTCTCACCGAACTCTCTGAGAAACACGACGTCGACGACGCCGTGTTTCTCGTCGATGGCTCCAGATCGTTACAA
GCTGTGTGTCAACGACATGGCTTCGATTTCAGATACGAAAAACATGGAAATCGGAATGCTGTTGAACGTGTCTTCAGAGAAATAAAACGACGAACTGTCT
GTTTCTCAAACTGTTTCAGCAACGCCGAAGCGGAAACAGCCGATGATTGGATCAGATCGTTCAGCTTCGCATGGAATCAGCTTATCTGAACACGATG
AGATTTTGTGGAGCGAGAAGCGACACCGCGACTGCTGATGAAGCTGAGTATTCAGCTGCATTTGGCTGGACTATCACTTTCGAATACTGTCTCGGTTCTT
GAAATATTCGGTGTCCAACGCGCTCGATCAACTGTCTATAATTGGGTTCACAAAGCAGATTTACAGCCCAAAGATGGACAGTCACCGGATCACGTTGCGG
TTGACGAAACTGTGATCCAACTCAACGGTGAGAGATATTGGCTGTACGCCGCTGTCGATCCACAAACGAACAAATTGCTTCATACGAAGCTTAGACCAAC
TACAACAAAGGTTCTCGCCCATGCATTTCTCACCGAACTCTCTGAGAAACACGACGTCGACGACGCCGTGTTTCTCGTCGATGGCTCCAGATCGTTACAA
GCTGTGTGTCAACGACATGGCTTCGATTTCAGATACGAAAAACATGGAAATCGGAATGCTGTTGAACGTGTCTTCAGAGAAATAAAACGACGAACTGTCT
GTTTCTCAAACTGTTTCAGCAACGCCGAAGCGGAAACAGCCGATGATTGGATCAGATCGTTCAGCTTCGCATGGAATCAGCTTATCTGAACACGATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 636 bp | 211 aa | 54 | 689 | + | No |
Chemistry : DDE
ORF sequence :
MPKNAGLGGSIDQIDLDFVEREATPRLLMKLSIQLHLAGLSLSNTVSVLEIFGVQRARSTVYNWVHKADLQPKDGQSPDHVAVDETVIQLNGERYWLYAA
VDPQTNKLLHTKLRPTTTKVLAHAFLTELSEKHDVDDAVFLVDGSRSLQAVCQRHGFDFRYEKHGNRNAVERVFREIKRRTVCFSNCFSNAEAETADDWI
RSFSFAWNQLI
VDPQTNKLLHTKLRPTTTKVLAHAFLTELSEKHDVDDAVFLVDGSRSLQAVCQRHGFDFRYEKHGNRNAVERVFREIKRRTVCFSNCFSNAEAETADDWI
RSFSFAWNQLI
Blast result :
Comments
ISH15 is 52% aa similar to ISPfu2. There are 2 complete copies in Haloarcula marismortui genome, one complete copy in Haloarcula marismortui plasmid pNG500 and one partial copy in Halobacterium sp. genome.
References
1] Baliga,N.S., Bonneau,R., Facciotti,M.T., Pan,M., Glusman,G., Deutsch,E.W., Shannon,P., Chiu,Y., Weng,R.S., Gan,R.R., Hung,P., Date,S.V., Marcotte,E., Hood,L. and Ng,W.V. (2004) Genome Res. 14 (11), 2221-2234