ISC774
- Family IS6
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002754 | ND | Sulfolobus solfataricus | Sulfolobus solfataricus P2 |
DNA section
IS Length : 778 bp
Ends
IR Length : 16
IRL : GTGTGGACAACAATTTTCTTAATTTAAAATAAATTGTGTAAATTGTTTTT
IRR : GTGTGGACAACAATTTCATGTAGTTGAAATGTTTGCATTTATCATCACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGTCTAGTTAA | TCAGATATGA | 0 | |
| CTAAACTAGA | TCCTTAAAAGTC | 0 |
DNA sequence
GTGTGGACAACAATTTTCTTAATTTAAAATAAATTGTGTAAATTGTTTTTGCGCGTGGAATAAGTCCTTTTGTTTAGCGTAATATCTTTTTGGGTGAGCA
GAGGATTTTTCTTGGTGAGCAAATCATGGACAAATGAAGTAGCCGCGCTCACTCAAGTTATTTTAACCTTAATGGAGTATATTAACCTTAAGCCTAGGTT
TCACGTTAGGGAGGAGATTGCTTTAAGCTTAGCGAGTTATCTAGCTGACTTATCCTCTTGGAGGACTACGTTGTTCCACTTCACCTTACTTTATGAGAGG
CTTGGTAGCCTCAGATATGCCGTATCCTTGAGTTACGCCATTGACGAGACTAAGGTTACTTGCGTTAGGGGTAATTACTACGTTTGGGTTATTAGGGAGG
TTGTAGCTAAAGCTATTTCCTTCTTCATGGTAACAAGCTTGAGGAGTGGTTTTCACGTAGTTATCATGAGGGGATTGCGAGTAAGTACTTTAGAATAGGG
TAGTTTACTTGCACGATGGTTTATCAGTTTACAATGCCTTTGATTGGTTTAATGTTGGGCACAAGAGGGTTACGTTAGGCGATTACACTGAAGAAGGGTC
TAGGAAATTAAAGCATAGAGTCTCTTCAATGGACTTCGCTTGTAATTCCAATAAGTTAATGAGTTGGTTCTCGACCTTCTTCTTAATCTACAACCTCCTT
TACATTCAAGTGTATTTAGTGGATAAGGGAGTGATGATAAATGCAAACATTTCAACTACATGAAATTGTTGTCCACAC
GAGGATTTTTCTTGGTGAGCAAATCATGGACAAATGAAGTAGCCGCGCTCACTCAAGTTATTTTAACCTTAATGGAGTATATTAACCTTAAGCCTAGGTT
TCACGTTAGGGAGGAGATTGCTTTAAGCTTAGCGAGTTATCTAGCTGACTTATCCTCTTGGAGGACTACGTTGTTCCACTTCACCTTACTTTATGAGAGG
CTTGGTAGCCTCAGATATGCCGTATCCTTGAGTTACGCCATTGACGAGACTAAGGTTACTTGCGTTAGGGGTAATTACTACGTTTGGGTTATTAGGGAGG
TTGTAGCTAAAGCTATTTCCTTCTTCATGGTAACAAGCTTGAGGAGTGGTTTTCACGTAGTTATCATGAGGGGATTGCGAGTAAGTACTTTAGAATAGGG
TAGTTTACTTGCACGATGGTTTATCAGTTTACAATGCCTTTGATTGGTTTAATGTTGGGCACAAGAGGGTTACGTTAGGCGATTACACTGAAGAAGGGTC
TAGGAAATTAAAGCATAGAGTCTCTTCAATGGACTTCGCTTGTAATTCCAATAAGTTAATGAGTTGGTTCTCGACCTTCTTCTTAATCTACAACCTCCTT
TACATTCAAGTGTATTTAGTGGATAAGGGAGTGATGATAAATGCAAACATTTCAACTACATGAAATTGTTGTCCACAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 694 bp | 231 aa | 70 | 763 | + | No |
Chemistry : DDE
ORF sequence :
MFSVISFWVSRGFFLVSKSWTNEVAALTQVILTLMEYINLKPRFHVREEIALSLASYLADLSSWRTTLFHFTLLYERLGSLRYAVSLSYAIDETKVTCVR
GNYYVWVIREVVAKAISFFMVTSLRSGFHVVIMRGLRVSTLENRVVYLHDGLSVYNAFDWFNVGHKRVTLGDYTEEGSRKLKHRVSSMDFACNSNKLMSW
FSTFFLIYNLLYIQVYLVDKGVMINANISTT
GNYYVWVIREVVAKAISFFMVTSLRSGFHVVIMRGLRVSTLENRVVYLHDGLSVYNAFDWFNVGHKRVTLGDYTEEGSRKLKHRVSSMDFACNSNKLMSW
FSTFFLIYNLLYIQVYLVDKGVMINANISTT
Blast result :
Comments
ISC774 is 69% aa similar to ISSto2. There are 2 complete and 10 partial copies (most of them are IRs) in Sulfolobus solfataricus P2 genome and 2 IRs in Sulfolobus acidocaldarius genome.
The two copies of ISC774 present 2 ORFs, a possible mutation generate a stop codon, the transposase is in silico translate.
The two copies of ISC774 present 2 ORFs, a possible mutation generate a stop codon, the transposase is in silico translate.
References
1] She,Q., Singh,R.K., Confalonieri,F., Zivanovic,Y., Allard,G., Awayez,M.J., Chan-Weiher,C.C., Clausen,I.G., Curtis,B.A., De Moors,A., Erauso,G., Fletcher,C., Gordon,P.M., Heikamp-de Jong,I., Jeffries,A.C., Kozera,C.J., Medina,N., Peng,X., Thi-Ngoc,H.P., Redder,P., Schenk,M.E., Theriault,C., Tolstrup,N., Charlebois,R.L., Doolittle,W.F., Duguet,M., Gaasterland,T., Garrett,R.A., Ragan,M.A., Sensen,C.W. and Van der Oost,J.(2001) Proc. Natl. Acad. Sci. U.S.A. 98 (14), 7835-7840