ISfinder Mirror Site

ISMja1

Family IS6
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISE703

Accession number	Transposition	Origin	Host
NC_000909	ND	Methanocaldococcus jannaschii	Methanocaldococcus jannaschii DSM 2661

DNA section

IS Length : 703 bp

Ends

IR Length : 19/22

IRL : GAGACTGTCAAGTTAAGTTTTTATCAAAATATTGATAAAAAATAATAAAA
IRR : GAGACTGTCAAGTTAATGATTTTACCCAATTGTAGAACATCATGAAGCTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

GAGACTGTCAAGTTAAGTTTTTATCAAAATATTGATAAAAAATAATAAAATATGAGGCTCACAATAGAAGTTATAAAGGAGAAAATCGTAGAGAGGAAGC
TTTTTAAAAGGAATAGGAAATCGATAGAGGTTAAAATCTTAGCAGGGCTTTTGTATTACCTCGGATTATCGTTAAGGAAGGTAAGTTTATTCCTTTCCCA
ATTCGAAGACATAAGCCACGAATCGGTTAGAATTTATTATCACAAGATTAAAGAAGTTTTAAACGAGCCAGAAAGAAAGGAAAGAAACTTAATTGCAATC
GATGAGATTAAACTAAAGGTTGGAGACAAATATATTTATGCATGGTCTGCCATCGATGTAGAAACGAAAGAATGCTTAGGAGTTTATATATCGAAGACAA
GAAATTACCTCGATACTATATTATTCGTTAAGAGTATATTAAAATTTTGCTCGAATAAGCCAAAGATTTTAGTTGACGGTGGAAAGTGGTATCCGTGGGC
GTTGCGAAAATTAGGCTTAGAATTCGAAAAAGTTAAATTCGGACTAAGAAATTGTGTAGAAAGCTTCTTCTCAGTGCTTAAACGAAGAACAAAAGCATTC
TTTAATAGGTTTCCTAATAATAGTAAATTCGATACAGTTATTAGCTGGATTAAAAGCTTCATGATGTTCTACAATTGGGTAAAATCATTAACTTGACAGT
CTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
642 bp	214 aa	52	693	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRLTIEVIKERIVERKLFKRNRKSIEVKILAGLLYYLGLSLRKVSLFLSQFEDISHESVRIYYHKIKEVLNEPERKERNLIAIDETKLKVGDKYIYAWSA
IDVETKECLGVYISKTRNYLDTILFVKSILKFCSNKPKILVDGGKWYPWALRKLGLEFERVKFGLRNCVESFFSVLKRRTKVFYNRFPNNSKFDTVISWI
KSFMMFYNWMKSLT

Blast result :

Comments

ISMja1 is 58% aa similar to ISPfu2. There are 2 complete copies and one partial copy in Methanocaldococcus jannaschii DSM 2661 genome and 3 partial copies in large extrachromosomal element.

References

1] Bult,C.J., White,O., Olsen,G.J., Zhou,L., Fleischmann,R.D., Sutton,G.G., Blake,J.A., FitzGerald,L.M., Clayton,R.A., Gocayne,J.D., Kerlavage,A.R., Dougherty,B.A., Tomb,J.-F.,
Adams,M.D., Reich,C.I., Overbeek,R., Kirkness,E.F., Weinstock,K.G., Merrick,J.M., Glodek,A., Scott,J.D., Geoghagen,N.S., Weidman,J.F., Fuhrmann,J.L., Nguyen,D.T., Utterback,T., Kelley,J.M.,
Peterson,J.D., Sadow,P.W., Hanna,M.C., Cotton,M.D., Hurst,M.A., Roberts,K.M., Kaine,B.B., Borodovsky,M., Klenk,H.P., Fraser,C.M., Smith,H.O., Woese,C.R. and Venter,J.C (1996) Science 273 (5278), 1058-1073