ISMbu3
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AADH00000000 | ND | Methanococcoides burtonii | Methanococcoides burtonii DSM 6242 |
DNA section
IS Length : 741 bp
Ends
IR Length : 13/15
IRL : GGCATTGGTATTTAAGTAGAGTTTATATAGTATCAGGGCCTATGTTTGTG
IRR : GGCATTGTTAATTAAACATCGCTAATTCTTTATTTCTGTACTTCATCAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAGTAAACTCTA | ATTTAGTG | GATCTTTCAACTCATGACAA | 8 |
DNA sequence
GGCATTGGTATTTAAGTAGAGTTTATATAGTATCAGGGCCTATGTTTGTGCATGAACTGTCCCAGGTGCAAGAGCTCAAATCATAAAAAGAACGGTAAAA
TCGATGGACGCCAACGCTACAAATGCCATGATTGTGGGTATAGCTATTCAGTAGAGATTAAATCAACTGCTAGTCCCATGTCTGTTAAACGGCAGGCTTT
ACAACTCTATCTCGAAGGATTAGGATTTCGTTCAATTGGACGTTTTTTAGGAGTTAGCCACGTTTCTGTCCAAAAATGGATAAAGAAGTTTGGTCGTGAA
TTAGAGGATCTAAAGAGCGAAAATGAAATATCGATTGTTGAATTAGATGAAATGCACACTTATATTGGGAACAAAAAAAATATTGTTGGATCTGGATTGC
TGTTGATAGAGATGGAAAAAGGTTCATCGACTGCTCTTTTGGTAGCAGGGGAACGGAAACAGGACTAAAACTCTGGAAAAAATTAAAGGGGAAGGAGATT
GGAGAAGTGATGACTGATCACTGGAGGGCATATGCAGAGTTTGTTCCAGAGAAAATTCACACTCAATCCAAAGCTGAAACATATACTGTTGAAGGATATA
ACAGCATATTCAGGCACTTTCTGGCAAGATTGAGAAGAAAGTCAAAGTGTTATACTAAGAGTCTTGAAATGTTGAAGTACTCTGTTCTTCTTTTGATGAA
GTACAGAAATAAAGAATTAGCGATGTTTAATTAACAATGCC
TCGATGGACGCCAACGCTACAAATGCCATGATTGTGGGTATAGCTATTCAGTAGAGATTAAATCAACTGCTAGTCCCATGTCTGTTAAACGGCAGGCTTT
ACAACTCTATCTCGAAGGATTAGGATTTCGTTCAATTGGACGTTTTTTAGGAGTTAGCCACGTTTCTGTCCAAAAATGGATAAAGAAGTTTGGTCGTGAA
TTAGAGGATCTAAAGAGCGAAAATGAAATATCGATTGTTGAATTAGATGAAATGCACACTTATATTGGGAACAAAAAAAATATTGTTGGATCTGGATTGC
TGTTGATAGAGATGGAAAAAGGTTCATCGACTGCTCTTTTGGTAGCAGGGGAACGGAAACAGGACTAAAACTCTGGAAAAAATTAAAGGGGAAGGAGATT
GGAGAAGTGATGACTGATCACTGGAGGGCATATGCAGAGTTTGTTCCAGAGAAAATTCACACTCAATCCAAAGCTGAAACATATACTGTTGAAGGATATA
ACAGCATATTCAGGCACTTTCTGGCAAGATTGAGAAGAAAGTCAAAGTGTTATACTAAGAGTCTTGAAATGTTGAAGTACTCTGTTCTTCTTTTGATGAA
GTACAGAAATAAAGAATTAGCGATGTTTAATTAACAATGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 417 bp | 138 aa | 52 | 468 | + | No |
Description : First part of the transposase
ORF sequence :
MNCPRCKSSNHKKNGKIDGRQRYKCHDCGYSYSVEIKSTASPMSVKRQALQLYLEGLGFRSIGRFLGVSHVSVQKWIKKFGRELEDLKSENEISIVELDE
MHTYIGNKKNIVGSGLLLIEMEKGSSTALLVAGERKQD
MHTYIGNKKNIVGSGLLLIEMEKGSSTALLVAGERKQD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 351 | 734 | + | No |
Description : Second part of the transposase
ORF sequence :
NAHLYWEQKKYCWIWIAVDRDGKRFIDCSFGSRGTETGLKLWKKLKGKEIGEVMTDHWRAYAEFVPEKIHTQSKAETYTVEGYNSIFRHFLARLRRKSKC
YTKSLEMLKYSVLLLMKYRNKELAMFN
YTKSLEMLKYSVLLLMKYRNKELAMFN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 683 bp | 226 aa | 52 | 734 | + | Yes |
Chemistry : DDE
ORF sequence :
MNCPRCKSSNHKKNGKIDGRQRYKCHDCGYSYSVEIKSTASPMSVKRQALQLYLEGLGFRSIGRFLGVSHVSVQKWIKKFGRELEDLKSENEISIVELDE
MHTYIGNKKYCWIWIAVDRDGKRFIDCSFGSRGTETGLKLWKKLKGKEIGEVMTDHWRAYAEFVPEKIHTQSKAETYTVEGYNSIFRHFLARLRRKSKCY
TKSLEMLKYSVLLLMKYRNKELAMFN
MHTYIGNKKYCWIWIAVDRDGKRFIDCSFGSRGTETGLKLWKKLKGKEIGEVMTDHWRAYAEFVPEKIHTQSKAETYTVEGYNSIFRHFLARLRRKSKCY
TKSLEMLKYSVLLLMKYRNKELAMFN
Blast result :
Comments
ISMbu3 is 92% aa similar to ISMac16.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. US DOE Joint Genome Institute (JGI-PGF) (20005) Direct submission GenBank.