ISSru3
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007677 | ND | Salinibacter ruber | Salinibacter ruber DSM 13855 |
DNA section
IS Length : 808 bp
Ends
IR Length : 8
IRL : GGTAGCGGCTCAACAAGTAGTGAGTCTTTCTGTTTGAACTGCGTCGCGTC
IRR : GGTAGCGGATAGATGTTAAGTGATGGCTTCGTTGTACCACTCCACGAACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCACAGATT | GATTTTTT | GTATGACCGACTCGCC | 8 |
DNA sequence
GGTAGCGGCTCAACAAGTAGTGAGTCTTTCTGTTTGAACTGCGTCGCGTCTGGCCCACCAGCCTGGTCTATCCGATGATCAAAGAGACTTACGAGTGTAG
AGAGTGTGGCTCCTCGAACATCGTCAAAAACGGACACAGCGCTAGCGGCTCTCAGCAGTATCACTGCAAGGACTGTGGAGCGCACAAGGTTCTCGATCCA
GAGCCGCGAGGCTACTCCGAGGAGGAGAAAGAGAAAATCCTCCGTGCTTACCGCGAGCGCGGGTCAAAGCGGGCAATCAGCCGGATATTCGGCATCAGCC
GCAATACATTGACCCGGTGGCTCAAAAAAAGGGACGAGAATCCGATTCAGTAGCCGAAGGGCTCCGGCCTGCTGAGGAAGGCGATGTCCTTGAACTCGAT
GAATGCTGGACGTATGTCCGAGAGCGGGCGAATAAACGGTGGCTGTGGGTTGCTCTGTGCCGGAGAACCCGGCAGGTCGTGGCATTTGTGATCGGAGACC
GTTCGGCCAGAACCTGTGCTCGGCTCTGGAGCCGGATTCCGGAGGAATACCGTCAGGGCAGAAGCTTCAGCGACTTCTGGAAGTCCTATCGCCCAGTGTT
TGCGGGCGACCCCAGCCACCGGCAGGTCGGAAAGTCCAGTGGTGAGATGGCCCACGTGGAAAGATTCTTCGGGCGACTGCGCCAGAAGCTGGCCCGGTAT
GTCCGCCGGACGCGAGCGGCCTCCGAGTCAGAACGGATGCTCCATCTGACGACGAAACTGTTCGTGGAGTGGTACAACGAAGCCATCACTTAACATCTAT
CCGCTACC
AGAGTGTGGCTCCTCGAACATCGTCAAAAACGGACACAGCGCTAGCGGCTCTCAGCAGTATCACTGCAAGGACTGTGGAGCGCACAAGGTTCTCGATCCA
GAGCCGCGAGGCTACTCCGAGGAGGAGAAAGAGAAAATCCTCCGTGCTTACCGCGAGCGCGGGTCAAAGCGGGCAATCAGCCGGATATTCGGCATCAGCC
GCAATACATTGACCCGGTGGCTCAAAAAAAGGGACGAGAATCCGATTCAGTAGCCGAAGGGCTCCGGCCTGCTGAGGAAGGCGATGTCCTTGAACTCGAT
GAATGCTGGACGTATGTCCGAGAGCGGGCGAATAAACGGTGGCTGTGGGTTGCTCTGTGCCGGAGAACCCGGCAGGTCGTGGCATTTGTGATCGGAGACC
GTTCGGCCAGAACCTGTGCTCGGCTCTGGAGCCGGATTCCGGAGGAATACCGTCAGGGCAGAAGCTTCAGCGACTTCTGGAAGTCCTATCGCCCAGTGTT
TGCGGGCGACCCCAGCCACCGGCAGGTCGGAAAGTCCAGTGGTGAGATGGCCCACGTGGAAAGATTCTTCGGGCGACTGCGCCAGAAGCTGGCCCGGTAT
GTCCGCCGGACGCGAGCGGCCTCCGAGTCAGAACGGATGCTCCATCTGACGACGAAACTGTTCGTGGAGTGGTACAACGAAGCCATCACTTAACATCTAT
CCGCTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 75 | 353 | + | No |
Description : First part of the transposase
ORF sequence :
MIKETYECRECGSSNIVKNGHSASGSQQYHCKDCGAHKVLDPEPRGYSEEEKEKILRAYRERGSKRAISRIFGISRNTLTRWLKKRDENPIQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 648 bp | 215 aa | 146 | 793 | + | No |
Description : Second part of the transposase
ORF sequence :
RLSAVSLQGLWSAQGSRSRAARLLRGGERENPPCLPRARVKAGNQPDIRHQPQYIDPVAQKKGRESDSVAEGLRPAEEGDVLELDECWTYVRERANKRWL
WVALCRRTRQVVAFVIGDRSARTCARLWSRIPEEYRQGRSFSDFWKSYRPVFAGDPSHRQVGKSSGEMAHVERFFGRLRQKLARYVRRTRAASESERMLH
LTTKLFVEWYNEAIT
WVALCRRTRQVVAFVIGDRSARTCARLWSRIPEEYRQGRSFSDFWKSYRPVFAGDPSHRQVGKSSGEMAHVERFFGRLRQKLARYVRRTRAASESERMLH
LTTKLFVEWYNEAIT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 719 bp | 238 aa | 75 | 793 | + | Yes |
Chemistry : DDE
ORF sequence :
MIKETYECRECGSSNIVKNGHSASGSQQYHCKDCGAHKVLDPEPRGYSEEEKEKILRAYRERGSKRAISRIFGISRNTLTRWLKKGRESDSVAEGLRPAE
EGDVLELDECWTYVRERANKRWLWVALCRRTRQVVAFVIGDRSARTCARLWSRIPEEYRQGRSFSDFWKSYRPVFAGDPSHRQVGKSSGEMAHVERFFGR
LRQKLARYVRRTRAASESERMLHLTTKLFVEWYNEAIT
EGDVLELDECWTYVRERANKRWLWVALCRRTRQVVAFVIGDRSARTCARLWSRIPEEYRQGRSFSDFWKSYRPVFAGDPSHRQVGKSSGEMAHVERFFGR
LRQKLARYVRRTRAASESERMLHLTTKLFVEWYNEAIT
Blast result :
Comments
ISSru3 is 52% aa similar to ISMae3 (insAB). The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Mongodin,E.F., Nelson,K.E., Daugherty,S., Deboy,R.T., Wister,J., Khouri,H., Weidman,J., Walsh,D.A., Papke,R.T., Sanchez Perez,G., Sharma,A.K., Nesbo,C.L., Macleod,D., Bapteste,E., Doolittle,W.F.,(2005) Proc. Natl. Acad. Sci. U.S.A. 102 (50), 18147-18152