ISGka4
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006509 | ND | Geobacillus kaustophilus | Geobacillus stearothermophilus T-6 Geobacillus kaustophilus HTA426 Geobacillus kaustophilus HTA426 plasmid pHTA426 |
DNA section
IS Length : 1635 bp
Ends
IR Length : 21/24
IRL : GTAACTATTCAGCCCGTACATAGAAAAAATTTACGAGAGCCGGTTTGTTC
IRR : GTATCTATTCAGCCCAGTACTAGCGAGATTTTCAGAATCCAGCACCAATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAATGACCCA | AACTCGTC | ATTTACTGAA | 8 |
| TTGGCAGTAG | AATTGAAC | AGTTTCAATC | 8 |
| AGGGAGAGGA | TTTGAACA | ACAATCATAT | 8 |
DNA sequence
GTAACTATTCAGCCCGTACATAGAAAAAATTTACGAGAGCCGGTTTGTTCGTACCTTCCTTGTCTATACTAATTACCATCATAGACATGGGAGGTGAACA
CAATGGCAAATGTTGCTTTTGGCTTCCAAGAAGCGGTCTTCACACTCGAAAGCATGGTGGCAAACATCGAACGTCAAGCACAGACCATCGAAAAACTCAT
CAACGAAAATAAACAGTTAAGACAAGAAAACCAGCAATTGAAAGCACGTATTGCAGAATTAGAAGCCCGTACGAAAAAAAACAGTACGAATAGTCATTTA
CCGCCGTCCTCTGACCGGTTTGGGGCGAAATCTCCTTCTCGCCAACCGTCGGAGAAACGGCCGGGAGGACAGCCAGGGCACCAAGGAACGACGCTCCGCC
AAGCACCGAATCCTGACCATCGAGTCCTTCACCGTGTGACCCAATGCAAAGGATGTGGTCACTCTTTAGAACATGTCGATCCACTTCAAGTAGACATTCG
CCAAGTGTTCGACCTCCCAGTGGTTCGCATGGAAGTCACTCAACATGAGCGGGAAATCAAGCGTTGTCCGGAATGCCGACTTGTCCAGCAGGCGGAGTTC
CCTTTCTATGTCACCAATCATGTACAATATGGACCGGTCATCACTTCACTCCTGTTATACTGGAATCACGCGCAGTTGATTCCGTGCGAACGGATTACGG
AGATGGTGAAAACATTATTTCATCATCCCATTAGTGCCGGAACGGTCGTGAACATGACGAGACGGTGGCAGCCTGTGTTAGAAGCAGCGATCGAAGAAAT
CGAAGCCGCGCTGCTCACCTCGAGCACGCTGCACGTCGATGAAACGAGCCTGCGTGTGAAGGGAACGAAACAATGGGTGCACGTCGCTTCCACTTCCAAG
GAAACCCGATACGGGATTCATCGCTCGCGTGGCAAACAAGCGACGGATGACATCGGGATTTTGCCACGGTACAAAGGAACGATGGTACATGATGCGTATT
CCGTATACCCGATGTATACGCAGGCGAGCCATGCCCTTTGCCACGCCCATCATCTCCGGGAGCTTCGGGAATATACCGAACTTTACCAGCACTCTTGGTC
GAACAAGATGGCCGAAGCACTGTTGGAGATGAAACAGGCAGTGGAGAAAGCGGGCGGAGCCTTGCCGGAAGAGAAAGTCCGGTATTGGGAAGCGGTGTAC
GACGAGCTTCTTTCGAATGGACGCCGGGAACTCGATGAACGTTGCCGACAAGGCAGCCATCTAGGTGTCCGCAAAGCGGAAAATTTCATCCGGCGCCTAG
AAAAGCGCAAGCAAGAAGCGCTTCTCTTCCTGCGAAAGAAAGAAGTGCCGTTTGACAATAACCAAGCCGAACGCGATTTACGGATGGTGAAAGTCAAACA
AAAAATCTCTGGAACGTTTCGTCAGGAAGACGATGCCGAGGCTTTCTGTGTCATCCGCAGCGTCGTTTCCACCCTGCAAAAACACGGAAAGCCGGTGTGG
GAATCGTTGCAAAGACTTCTAAGCGGAGAGCCTCTTCAAACGATTCTCCATTCCTCCTAGGGCATTTTCGATACTGGGAATGCCCTATTGGTGCTGGATT
CTGAAAATCTCGCTAGTACTGGGCTGAATAGATAC
CAATGGCAAATGTTGCTTTTGGCTTCCAAGAAGCGGTCTTCACACTCGAAAGCATGGTGGCAAACATCGAACGTCAAGCACAGACCATCGAAAAACTCAT
CAACGAAAATAAACAGTTAAGACAAGAAAACCAGCAATTGAAAGCACGTATTGCAGAATTAGAAGCCCGTACGAAAAAAAACAGTACGAATAGTCATTTA
CCGCCGTCCTCTGACCGGTTTGGGGCGAAATCTCCTTCTCGCCAACCGTCGGAGAAACGGCCGGGAGGACAGCCAGGGCACCAAGGAACGACGCTCCGCC
AAGCACCGAATCCTGACCATCGAGTCCTTCACCGTGTGACCCAATGCAAAGGATGTGGTCACTCTTTAGAACATGTCGATCCACTTCAAGTAGACATTCG
CCAAGTGTTCGACCTCCCAGTGGTTCGCATGGAAGTCACTCAACATGAGCGGGAAATCAAGCGTTGTCCGGAATGCCGACTTGTCCAGCAGGCGGAGTTC
CCTTTCTATGTCACCAATCATGTACAATATGGACCGGTCATCACTTCACTCCTGTTATACTGGAATCACGCGCAGTTGATTCCGTGCGAACGGATTACGG
AGATGGTGAAAACATTATTTCATCATCCCATTAGTGCCGGAACGGTCGTGAACATGACGAGACGGTGGCAGCCTGTGTTAGAAGCAGCGATCGAAGAAAT
CGAAGCCGCGCTGCTCACCTCGAGCACGCTGCACGTCGATGAAACGAGCCTGCGTGTGAAGGGAACGAAACAATGGGTGCACGTCGCTTCCACTTCCAAG
GAAACCCGATACGGGATTCATCGCTCGCGTGGCAAACAAGCGACGGATGACATCGGGATTTTGCCACGGTACAAAGGAACGATGGTACATGATGCGTATT
CCGTATACCCGATGTATACGCAGGCGAGCCATGCCCTTTGCCACGCCCATCATCTCCGGGAGCTTCGGGAATATACCGAACTTTACCAGCACTCTTGGTC
GAACAAGATGGCCGAAGCACTGTTGGAGATGAAACAGGCAGTGGAGAAAGCGGGCGGAGCCTTGCCGGAAGAGAAAGTCCGGTATTGGGAAGCGGTGTAC
GACGAGCTTCTTTCGAATGGACGCCGGGAACTCGATGAACGTTGCCGACAAGGCAGCCATCTAGGTGTCCGCAAAGCGGAAAATTTCATCCGGCGCCTAG
AAAAGCGCAAGCAAGAAGCGCTTCTCTTCCTGCGAAAGAAAGAAGTGCCGTTTGACAATAACCAAGCCGAACGCGATTTACGGATGGTGAAAGTCAAACA
AAAAATCTCTGGAACGTTTCGTCAGGAAGACGATGCCGAGGCTTTCTGTGTCATCCGCAGCGTCGTTTCCACCCTGCAAAAACACGGAAAGCCGGTGTGG
GAATCGTTGCAAAGACTTCTAAGCGGAGAGCCTCTTCAAACGATTCTCCATTCCTCCTAGGGCATTTTCGATACTGGGAATGCCCTATTGGTGCTGGATT
CTGAAAATCTCGCTAGTACTGGGCTGAATAGATAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1458 bp | 485 aa | 103 | 1560 | + | No |
Chemistry : DDE
ORF sequence :
MANVAFGFQEAVFTLESMVANIERQAQTIEKLINENKQLRQENQQLKARIAELEARTKKNSTNSHLPPSSDRFGAKSPSRQPSEKRPGGQPGHQGTTLRQ
APNPDHRVLHRVTQCKGCGHSLEHVDPLQVDIRQVFDLPVVRMEVTQHEREIKRCPECRLVQQAEFPFYVTNHVQYGPVITSLLLYWNHAQLIPCERITE
MVKTLFHHPISAGTVVNMTRRWQPVLEAAIEEIEAALLTSSTLHVDETSLRVKGTKQWVHVASTSKETRYGIHRSRGKQATDDIGILPRYKGTMVHDAYS
VYPMYTQASHALCHAHHLRELREYTELYQHSWSNKMAEALLEMKQAVEKAGGALPEEKVRYWEAVYDELLSNGRRELDERCRQGSHLGVRKAENFIRRLE
KRKQEALLFLRKKEVPFDNNQAERDLRMVKVKQKISGTFRQEDDAEAFCVIRSVVSTLQKHGKPVWESLQRLLSGEPLQTILHSS
APNPDHRVLHRVTQCKGCGHSLEHVDPLQVDIRQVFDLPVVRMEVTQHEREIKRCPECRLVQQAEFPFYVTNHVQYGPVITSLLLYWNHAQLIPCERITE
MVKTLFHHPISAGTVVNMTRRWQPVLEAAIEEIEAALLTSSTLHVDETSLRVKGTKQWVHVASTSKETRYGIHRSRGKQATDDIGILPRYKGTMVHDAYS
VYPMYTQASHALCHAHHLRELREYTELYQHSWSNKMAEALLEMKQAVEKAGGALPEEKVRYWEAVYDELLSNGRRELDERCRQGSHLGVRKAENFIRRLE
KRKQEALLFLRKKEVPFDNNQAERDLRMVKVKQKISGTFRQEDDAEAFCVIRSVVSTLQKHGKPVWESLQRLLSGEPLQTILHSS
Blast result :
Comments
ISGka4 is 74% aa similar to ISBst12.
There are one complete copy in Geobacillus kaustophilus HTA426 plasmid pHTA426 and one complete copy in Geobacillus kaustophilus HTA426 genome.
There are one complete copy in Geobacillus kaustophilus HTA426 plasmid pHTA426 and one complete copy in Geobacillus kaustophilus HTA426 genome.
References
1] Takami,H., Takaki,Y., Chee,G.J., Nishi,S., Shimamura,S., Suzuki,H., Matsui,S. and Uchiyama,I. (2004) Nucleic Acids Res. 32 (21), 6292-6303