ISFsp3

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY008259 ND Frankia sp.
Frankia sp. Ar15
DNA section
IS Length : 2209 bp

Ends


IR Length : 27/34

IRL : TGTCGCCGCCTCCTGAAAACTGACCCCCCAGGGGGCCGCGAAAACTGACC
IRR : TGTCAAGGCTTCCTGAAAACTGACCCCGTGGGGGTCTCTGAAATTTGACC

Insertion site


Left flankDirect repeatRight flankDR Length
ATCCGGGTCAACGTCCACCACGGGCGGAGAGCAGCGCCGGG0

DNA sequence

TGTCGCCGCCTCCTGAAAACTGACCCCCCAGGGGGCCGCGAAAACTGACCCCCTGCTGGTGTAGGAGGGGTGCTGAAGGTGGAGGACTGGGCGGAGATCC
GCAGGTTGCGTCGAGCTGAGGGCGTTCCGATCAAGGAGATCGCTCGACGGCTGGGTGTGGCGCGGAACACGGTGCGGGCGGCGTTGGCGTCGGACCGGCC
GCCGCAGTACGAACGGGCGCCGCGCGGGTCGGTGGTCGATCCGTTCGAGCCGGTGATCCGGGCGTTGCTGGCGGAGTGGCCGCGGATGCCGGCGCCGGTG
ATCGCGCAGCGGATCGGCTGGCCGTATTCGCTGTCGCCGTTGAAGAAGCGATTGACGGTGATCCGGCCGGAGTATGTCGGGATCGACCCGGTGGACCGGA
TGGTGTATGAGCCGGGGGAGTTCGCGCATTGTGATCTGTGGTTCCCGGAGCCGGTGATTCCGGTGGGTGCGGGGCAGGAAAGGGTGCTTCCGGTGCTGGT
GATGACGTTGGCGTTCTCCCGGTTCCTGACCGCGACGATGATTCCCTCGCGGCAGGCCGGTGACATTCTCGCGGGCATGTGGCTGCTGATCGGCCGGGTC
GGGCGGGTCACGAAGACGCTGGTGTGGGATCGGGAATCGGCGATCGGGGGGACCGGCCGGGTGTCGGCGCCGGCGGCGGGGTTCGCGGGAACGTTGGCGA
CCCAGATCAGGCTTGCGCCGCCACGGGACCCGGAATATAAGGGTATCGTCGAGCGGGCCAACGGCTATTTCGAGACGTCGTTTCTGCCGGGCCGGCGGTT
CGTCTCCCCGGAGGATTTCAACATCCAGCTGGCTGAATGGCTGACGCTGGCGAATGCCCGCACCGTGCGGTCGGTCGGGGGCCGTCCGGTCGATCTGCTG
GAGACCGATCTGCGGTCGATGCTGGAACTGCCACCGGTCGACCCGCTGACCGGCCTTTCCGCCCGGGTCCGGCTCGGCCGGGACTACTACGTGCGGGTCG
ACACCGTCGACTACTCCGTCGACCCGCGGGCGATCGGCCGGTTCGTCGACGTGACCGCCTCGCTGGACACGGTGGCGGTGACCTGCGACGGCCAGCCCGT
GGCCCGTCATGCCCGCTCGTGGGCCCGCCACGGTGTCATCACCGATCCCGAGCATGCCGCGGCCGCCGCGCGGATGCGCCAGGCCCTGGCCGAGGACCGC
CGGCGCCGGGCCGCGGCAACACGCCACCACGGCGACGGCCACCCGGTCAGCATGCGGGCGCTGCCGGACTACGACGCCCTGTTCGGCGTCGACTTCACCC
CCACACCGTCCGAGAAGAAAGCGAGCAGCGAATGACCACAGCCACCATACCGGCGGCACCGGCCGGGAAAACCTCCGACGGCATGCCAGCAATGATCGCC
TATCTGACCCGGGTGTTGAAAACCCCGACGATCGGCGCGTTCTGGGAAGAACTCGCCATCCAGGCCCGCGAAGAGAACTGGTCCCACGAAGAATACCTCG
CCGCTCTGCTGCAGCGCCAGGTCGCCGACCGCGAGTCCAAAGGCACCGTCATGCGGATCCGCACCGCGCACTTCCCGACCGTCAAAACGTTGGAGGACTT
CAACCTCGACCACCTCCCCTCACTTCGCCGCGACGTTCTCGCCCATCTGGCCACCAGCACCTACATCGCCAAAGCGGGAAACGTCGTCCTCCTCGGCCCG
CCCGGCGTCGGGAAGACCCACCTTGCCATCGGCCTGGGCGTCAAAGCAACCCACGCCGGCTACTCCGTCCTGTTCGACACCGCCAGCAACTGGATAACCC
GCCTCGCCGACGCCCACCACGCCGGCCGTCTCGACGAAGAACTCAGAAAGATCCGCCGCTACAAACTTATCATCATCGACGAAGTCGGCTACATCCCCTT
CGACCAGGACGCAGCGAACCTGTTCTTCCAGCTCATCGCCTCCCGCTACGAACAGGGCTCGGTCCTGGTCACCTCGAACCTCCCCTTCGGCCGCTGGGGC
GAGACCTTCTCCGACGACGTCGTCGCCGCCGCCATGATCGACCGGCTCGTCCACCACGCCGAGGTCCTCACCCTCGCCGGCGACTCCTACCGCACCCGCC
AACGCCGCGAGCTCCTCGCCAAGGACCGACCCAACCACAACTGATCAACCAGGAATGGGGGTCAAATTTCAGAGACCCCCACGGGGTCAGTTTTCAGGAA
GCCTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
936 bp311 aa4001335+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVYEPGEFAHCDLWFPEPVIPVGAGQERVLPVLVMTLAFSRFLTATMIPSRQAGDILAGMWLLIGRVGRVTKTLVWDRESAIGGTGRVSAPAAGFAGTLA
TQIRLAPPRDPEYKGIVERANGYFETSFLPGRRFVSPEDFNIQLAEWLTLANARTVRSVGGRPVDLLETDLRSMLELPPVDPLTGLSARVRLGRDYYVRV
DTVDYSVDPRAIGRFVDVTASLDTVAVTCDGQPVARHARSWARHGVITDPEHAAAAARMRQALAEDRRRRAAATRHHGDGHPVSMRALPDYDALFGVDFT
PTPSEKKASSE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1308 bp435 aa8372144+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MADAGECPHRAVGRGPSGRSAGDRSAVDAGTATGRPADRPFRPGPARPGLLRAGRHRRLLRRPAGDRPVRRRDRLAGHGGGDLRRPARGPSCPLVGPPRC
HHRSRACRGRRADAPGPGRGPPAPGRGNTPPRRRPPGQHAGAAGLRRPVRRRLHPHTVREESEQRMTTATIPAAPAGKTSDGMPAMIAYLTRVLKTPTIG
AFWEELAIQAREENWSHEEYLAALLQRQVADRESKGTVMRIRTAHFPTVKTLEDFNLDHLPSLRRDVLAHLATSTYIAKAGNVVLLGPPGVGKTHLAIGL
GVKATHAGYSVLFDTASNWITRLADAHHAGRLDEELRKIRRYKLIIIDEVGYIPFDQDAANLFFQLIASRYEQGSVLVTSNLPFGRWGETFSDDVVAAAM
IDRLVHHAEVLTLAGDSYRTRQRRELLAKDRPNHN

 

Blast result :
Comments
ISFsp3 is 57% (ORF A) aaa similar to ISMt3 and 75% (ORF B) to ISLxx1.
References
1] Bock,J.V., Battershell,T., Wiggington,J., John,T.R. and Johnson,J.D. (2001) Microbiology 147 (Pt 2), 499-506