ISMma6
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1093 bp
Ends
IR Length : 20/29
IRL : GTCTTCACATAAATTATTTACATTTATAAAACCATCTTTATACTTATGGG
IRR : GTCAACTCACGAATTCATTACACTTTTAATTTGTTCATCAAATATTTTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCGGTTTA | TA | TAATACGCTTAC | 2 |
DNA sequence
GTCTTCACATAAATTATTTACATTTATAAAACCATCTTTATACTTATGGGGAGACCTGAAATCTATCATATTGACCAAAAGATTTCTCTTGAGAAACTTG
ATTGGCTCATAAAGATTGAAAGAAATACTAAAGTTCAACAGAAGTTATATTTTATTCGGTTTAGATATTTAGGCGATTCTATAGAGGAAGCAACATCGAG
ATTAGGAGTCACTAAAAGAATTGGTTATTATTGGCAAAATAGATGGAATGAACAAGGTTATGAGGGTTTACTTCACAAATCTGGTGCAGGTAGACCTTCT
TATTTGAGTGATGAGAAAATACTTGAACTCAAGTCAATACTGGAAAGTAAAGACTTTTGGACTACAGATGAAGTTAAAGATTTGATAAAAGATAAGTTTG
GCATTGATTATTGCTTGAATTCCATAAGAAAACTATTGAAGAAAATAGGTATGCACTATAATATTCCTTATTGTTTGGACTATAGAAGACCTGAAAATGC
AGAAGAGATTTTAAAAAAATTTAGAAAATGCAATAAAAGAAAAAACTTCTCCTGATAAACATTATGTAATAGGCTTCCTTGACGAATCTTCACCACAAAC
AGCACCAGACACACCAAGGCTGTGGTCATTCGTGAAAAAACCTAAAATGTTCAAAAATACAAAGAAGATGAAAGCAAATGCAAATGCTTTCTATGCTATT
AATGGGAATAGCGTTATCGATTTTAAAGAAAGTTCAAAGGCAAAAGATGTATGTGAATTTTTAGGAAGAATAAAAGAAGAAAACGAGTATGACCCAATAG
TTGTACTGGACAATTCTAAAACACATCATGCAGACATTACAATTAAAAAGGCGAAACAATTGGATATTACATTAGTGTTCCTGCCACCATACTCACCCGA
CCTAAACCCAATTGAATTTATATGGAAGAGTGTAAGGAAGGAAATCTTAAAAGAATTTATTGAATCAGTTACACAACTAAGAGACTTGATTAAAAATGAG
TATATGAAGTTAGCTAAATCAAAATCTTTTGCAAATAATTGGATGAAAATATTTGATGAACAAATTAAAAGTGTAATGAATTCGTGAGTTGAC
ATTGGCTCATAAAGATTGAAAGAAATACTAAAGTTCAACAGAAGTTATATTTTATTCGGTTTAGATATTTAGGCGATTCTATAGAGGAAGCAACATCGAG
ATTAGGAGTCACTAAAAGAATTGGTTATTATTGGCAAAATAGATGGAATGAACAAGGTTATGAGGGTTTACTTCACAAATCTGGTGCAGGTAGACCTTCT
TATTTGAGTGATGAGAAAATACTTGAACTCAAGTCAATACTGGAAAGTAAAGACTTTTGGACTACAGATGAAGTTAAAGATTTGATAAAAGATAAGTTTG
GCATTGATTATTGCTTGAATTCCATAAGAAAACTATTGAAGAAAATAGGTATGCACTATAATATTCCTTATTGTTTGGACTATAGAAGACCTGAAAATGC
AGAAGAGATTTTAAAAAAATTTAGAAAATGCAATAAAAGAAAAAACTTCTCCTGATAAACATTATGTAATAGGCTTCCTTGACGAATCTTCACCACAAAC
AGCACCAGACACACCAAGGCTGTGGTCATTCGTGAAAAAACCTAAAATGTTCAAAAATACAAAGAAGATGAAAGCAAATGCAAATGCTTTCTATGCTATT
AATGGGAATAGCGTTATCGATTTTAAAGAAAGTTCAAAGGCAAAAGATGTATGTGAATTTTTAGGAAGAATAAAAGAAGAAAACGAGTATGACCCAATAG
TTGTACTGGACAATTCTAAAACACATCATGCAGACATTACAATTAAAAAGGCGAAACAATTGGATATTACATTAGTGTTCCTGCCACCATACTCACCCGA
CCTAAACCCAATTGAATTTATATGGAAGAGTGTAAGGAAGGAAATCTTAAAAGAATTTATTGAATCAGTTACACAACTAAGAGACTTGATTAAAAATGAG
TATATGAAGTTAGCTAAATCAAAATCTTTTGCAAATAATTGGATGAAAATATTTGATGAACAAATTAAAAGTGTAATGAATTCGTGAGTTGAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 510 bp | 169 aa | 46 | 555 | + | No |
Description : First part of the transposase
ORF sequence :
MGRPEIYHIDQKISLEKLDWLIKIERNTKVQQKLYFIRFRYLGDSIEEATSRLGVTKRIGYYWQNRWNEQGYEGLLHKSGAGRPSYLSDEKILELKSILE
SKDFWTTDEVKDLIKDKFGIDYCLNSIRKLLKKIGMHYNIPYCLDYRRPENAEEILKKFRKCNKRKNFS
SKDFWTTDEVKDLIKDKFGIDYCLNSIRKLLKKIGMHYNIPYCLDYRRPENAEEILKKFRKCNKRKNFS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 420 bp | 105 aa | 668 | 1087 | + | No |
Description : Second part of the transposase
ORF sequence :
MKANANAFYAINGNSVIDFKESSKAKDVCEFLGRIKEENEYDPIVVLDNSKTHHADITIKKAKQLDITLVFLPPYSPDLNPIEFIWKSVRKEILKEFIES
VTQLR
VTQLR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1042 bp | 0 aa | 46 | 1087 | + | Yes |
Chemistry : DDE
ORF sequence :
Blast result :
Comments
ORF1 is 69% aa similar to ISA1083_aa1
ORF2 is 70% aa similar to ISA1083_aa2
Note that most IS630 family members have only a single ORF.
ORF2 is 70% aa similar to ISA1083_aa2
Note that most IS630 family members have only a single ORF.
References
1] Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C.,,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G. (2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461