ISBcen16
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1478 bp
Ends
IR Length : 22/25
IRL : TGAACTGCACCCCAAAAGTTGGACACCGATCCAATCTTTGGGGTGTTTTT
IRR : TGAACTGACCCCGCAAAGTTGGACGGTTAACCGCTAAGCGCCCAAGGGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAAAGCGTGGCGG | GTG | CCACGCTTTTTT | 3 |
DNA sequence
TGAACTGCACCCCAAAAGTTGGACACCGATCCAATCTTTGGGGTGTTTTTCATGACGAAGTACGACGAGCAATTCAAGCAACAGGTGGTCAGCGCATATT
TGCGTGGCGATAGTGGTTTTGACGCACTGGCAGCCCGTCACGGCATCCATTCCGGAATGTTGCAGCGTTGGGTTGCGGCGTATCGCGCGCATGGCCGTGC
GGGACTGCAGAGCAAATACAGTAGTTACGACGCACCATTCAAGCTGCGGGTGTTGCAGCACATGCAGCGCGATGAGTTGTCGCTGCGTGAAGTCTCGGCG
CTGTATGACATTCGTGATGCGGGTAGTGTGGCCCGTTGGGCGTGTCTGTATGATGAAGGCGGTATAAACGCTTTGACGCCCCGGCCGCGAGGGCGCTCCC
GACAGATGGTCACATCACCTCCACCCAAACCCACCGAAGCCGGCACACCGGATGAACGCTCGCGCGAGGAATTGCTCAAGGAAAACGAGTATCTGCGTGC
GGAGGTGGCGTACCTAAAAAAGCTCGATGCGCTGCTGGCCAAGAAGCAGGCAGCGCAAAAGAAAAAACGCAAATAGTGCGCGAGCTCAGACAGCATCATC
CGGTAGCAGCACTTCTGAAGGTGGCCGGTTTGGCGCGCAGCACGTTCTATTACCAGCTCGGGACGCTGAATGCCGACGATCGTCACGCTGATCTGAAGGC
AAAGATCCGGACGGTGTTCGACCATCACAAGGGACGCTACGGCTATCGACGCGTCACGGCCGCGATTCGGCAAGCCGGGCATCTCGTCAACCACAAGGCC
GTGCAGCGGCTGATGCAGCAGTTGCAACTGAAGTCCTTGGTGCGCCCGAAGAAATACCGTTCCTGGCAAGGTGAGGTCGGTCAGGCCGCGCCCAACCTGC
TGCAACGTCAGTTCAGTGCCACGCAGGCCAACCAGAAGTGGGTGACGGATGTGACGGAATTCAACGTCGGCGGGCAGAAGCTGTATCTGTCGCCGGTGAT
GGATCTATACAACGGCGAGATCGTGGCTTACCAGATGGATCAACGTCCAACCTTCAAGTTGGTCAGCGACATGCTGAAGAAGGCGCTAGCCAAGCTGCGA
GGCAAGGACAAACCGTTATTGCATTCGGATCAAGGCTGGCAATACCGGATGCCGGCGTATCGCCGGCAACTGGAGGAGCGCAAGCTGACCCAGAGCATGT
CGCGCAAGGGCAACTGTCTCGACAATGCCGCGATGGAAAGCTTCTTCGGTACGCTCAAGTCCGAGTGCTACAAGGGTCATCGTTTCACTTGCGTGAGCCA
TTTACGCGACACGCTCGACCACTACATCCACTACTACAACCACGAACGCATCAAGCTCAAATTGAAAGGGCTGAGTCCCGTGCAATACAGGACTCAGCCC
TTGGGCGCTTAGCGGTTAACCGTCCAACTTTGCGGGGTCAGTTCA
TGCGTGGCGATAGTGGTTTTGACGCACTGGCAGCCCGTCACGGCATCCATTCCGGAATGTTGCAGCGTTGGGTTGCGGCGTATCGCGCGCATGGCCGTGC
GGGACTGCAGAGCAAATACAGTAGTTACGACGCACCATTCAAGCTGCGGGTGTTGCAGCACATGCAGCGCGATGAGTTGTCGCTGCGTGAAGTCTCGGCG
CTGTATGACATTCGTGATGCGGGTAGTGTGGCCCGTTGGGCGTGTCTGTATGATGAAGGCGGTATAAACGCTTTGACGCCCCGGCCGCGAGGGCGCTCCC
GACAGATGGTCACATCACCTCCACCCAAACCCACCGAAGCCGGCACACCGGATGAACGCTCGCGCGAGGAATTGCTCAAGGAAAACGAGTATCTGCGTGC
GGAGGTGGCGTACCTAAAAAAGCTCGATGCGCTGCTGGCCAAGAAGCAGGCAGCGCAAAAGAAAAAACGCAAATAGTGCGCGAGCTCAGACAGCATCATC
CGGTAGCAGCACTTCTGAAGGTGGCCGGTTTGGCGCGCAGCACGTTCTATTACCAGCTCGGGACGCTGAATGCCGACGATCGTCACGCTGATCTGAAGGC
AAAGATCCGGACGGTGTTCGACCATCACAAGGGACGCTACGGCTATCGACGCGTCACGGCCGCGATTCGGCAAGCCGGGCATCTCGTCAACCACAAGGCC
GTGCAGCGGCTGATGCAGCAGTTGCAACTGAAGTCCTTGGTGCGCCCGAAGAAATACCGTTCCTGGCAAGGTGAGGTCGGTCAGGCCGCGCCCAACCTGC
TGCAACGTCAGTTCAGTGCCACGCAGGCCAACCAGAAGTGGGTGACGGATGTGACGGAATTCAACGTCGGCGGGCAGAAGCTGTATCTGTCGCCGGTGAT
GGATCTATACAACGGCGAGATCGTGGCTTACCAGATGGATCAACGTCCAACCTTCAAGTTGGTCAGCGACATGCTGAAGAAGGCGCTAGCCAAGCTGCGA
GGCAAGGACAAACCGTTATTGCATTCGGATCAAGGCTGGCAATACCGGATGCCGGCGTATCGCCGGCAACTGGAGGAGCGCAAGCTGACCCAGAGCATGT
CGCGCAAGGGCAACTGTCTCGACAATGCCGCGATGGAAAGCTTCTTCGGTACGCTCAAGTCCGAGTGCTACAAGGGTCATCGTTTCACTTGCGTGAGCCA
TTTACGCGACACGCTCGACCACTACATCCACTACTACAACCACGAACGCATCAAGCTCAAATTGAAAGGGCTGAGTCCCGTGCAATACAGGACTCAGCCC
TTGGGCGCTTAGCGGTTAACCGTCCAACTTTGCGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 174 aa | 52 | 573 | + | No |
Description : First part of the transposase
ORF sequence :
MTKYDEQFKQQVVSAYLRGDSGFDALAARHGIHSGMLQRWVAAYRAHGRAGLQSKYSSYDAPFKLRVLQHMQRDELSLREVSALYDIRDAGSVARWACLY
DEGGINALTPRPRGRSRQMVTSPPPKPTEAGTPDERSREELLKENEYLRAEVAYLKKLDALLAKKQAAQKKKRK
DEGGINALTPRPRGRSRQMVTSPPPKPTEAGTPDERSREELLKENEYLRAEVAYLKKLDALLAKKQAAQKKKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 456 | 1412 | + | No |
Description : Second part of the transposase
ORF sequence :
TLARGIAQGKRVSACGGGVPKKARCAAGQEAGSAKEKTQIVRELRQHHPVAALLKVAGLARSTFYYQLGTLNADDRHADLKAKIRTVFDHHKGRYGYRRV
TAAIRQAGHLVNHKAVQRLMQQLQLKSLVRPKKYRSWQGEVGQAAPNLLQRQFSATQANQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDQRPTF
KLVSDMLKKALAKLRGKDKPLLHSDQGWQYRMPAYRRQLEERKLTQSMSRKGNCLDNAAMESFFGTLKSECYKGHRFTCVSHLRDTLDHYIHYYNHERIK
LKLKGLSPVQYRTQPLGA
TAAIRQAGHLVNHKAVQRLMQQLQLKSLVRPKKYRSWQGEVGQAAPNLLQRQFSATQANQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDQRPTF
KLVSDMLKKALAKLRGKDKPLLHSDQGWQYRMPAYRRQLEERKLTQSMSRKGNCLDNAAMESFFGTLKSECYKGHRFTCVSHLRDTLDHYIHYYNHERIK
LKLKGLSPVQYRTQPLGA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1361 bp | 453 aa | 52 | 1412 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKYDEQFKQQVVSAYLRGDSGFDALAARHGIHSGMLQRWVAAYRAHGRAGLQSKYSSYDAPFKLRVLQHMQRDELSLREVSALYDIRDAGSVARWACLY
DEGGINALTPRPRGRSRQMVTSPPPKPTEAGTPDERSREELLKENEYLRAEVAYLKKARCAAGQEAGSAKEKTQIVRELRQHHPVAALLKVAGLARSTFY
YQLGTLNADDRHADLKAKIRTVFDHHKGRYGYRRVTAAIRQAGHLVNHKAVQRLMQQLQLKSLVRPKKYRSWQGEVGQAAPNLLQRQFSATQANQKWVTD
VTEFNVGGQKLYLSPVMDLYNGEIVAYQMDQRPTFKLVSDMLKKALAKLRGKDKPLLHSDQGWQYRMPAYRRQLEERKLTQSMSRKGNCLDNAAMESFFG
TLKSECYKGHRFTCVSHLRDTLDHYIHYYNHERIKLKLKGLSPVQYRTQPLGA
DEGGINALTPRPRGRSRQMVTSPPPKPTEAGTPDERSREELLKENEYLRAEVAYLKKARCAAGQEAGSAKEKTQIVRELRQHHPVAALLKVAGLARSTFY
YQLGTLNADDRHADLKAKIRTVFDHHKGRYGYRRVTAAIRQAGHLVNHKAVQRLMQQLQLKSLVRPKKYRSWQGEVGQAAPNLLQRQFSATQANQKWVTD
VTEFNVGGQKLYLSPVMDLYNGEIVAYQMDQRPTFKLVSDMLKKALAKLRGKDKPLLHSDQGWQYRMPAYRRQLEERKLTQSMSRKGNCLDNAAMESFFG
TLKSECYKGHRFTCVSHLRDTLDHYIHYYNHERIKLKLKGLSPVQYRTQPLGA
Blast result :
Comments
1 copy within J2315. Ends defined by inverted repeats.
ISBcen16 is 67%(ORF1) aa similar to IS1221A and 81% (ORF2) to ISRso11.
ISBcen16 is interrupted by ISBcen17.
ISBcen16 is 67%(ORF1) aa similar to IS1221A and 81% (ORF2) to ISRso11.
ISBcen16 is interrupted by ISBcen17.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth Smith, H. (2006) Direct submission.
2] Seth Smith, H. (2006) Direct submission.