ISBcen15
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1239 bp
Ends
IR Length : 47-48
IRL : TGACCCTAGCCCACAAAACTGTCCCATTTGAAAGTGGAAAATTCCGGCAT
IRR : TGAGGCTGGCCCAATTTCCGTCCCGGTCTTAGTTGGAAAATTCCGGCTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCGAGCCGG | CGATTACTAG | 0 | |
| CTTTTCCTGC | CTGCTCAGCC | 0 | |
| AAATCATCAC | GCCGAACCGA | 0 |
DNA sequence
TGACCCTAGCCCACAAAACTGTCCCATTTGAAAGTGGAAAATTCCGGCATGACGTTGTCATCGAGACCAGGAGGTCATGCCGTGAAGAAGAGCAAGTTCA
CCGAAGAGCAGATCGCATACGCGCTGAAGCAGGCCGAGCTGGGTACGCCTGTCGCGGAGGTCTGCCGCAAGATGGGGATCAGCGACGCGACGTTTTACAA
CTGGCGGACGAAGTACGGCGGGCTGTCACCCTCGGAACTGCGCCGGCTGAAGCAGCTTGAGGAAGAGAACGCGAAGCTCAAGCGGCTCGTGGCCGATCTG
TCGCTGGACAAGGCCATGCTGCAGGATGTGCTGTCAAAAAAGCTCTGAAGCCTTCCCGGCGCCGTGATCTGGTCACGGACTTGATGCAGCGTTTCGGCGC
GAGCCAGCGGCAAACCTGCGCATTGTTGCAGTTGTCCCGCACGGTCTACCGTTACGAATCGGTCGCGCGGGATCAAAGCGCGCTGGAGATGCGCATCAAG
GAGATCACGGAAGTGCGGGTGCATTATGGTGCGCCCCGCGTGTACGTGATGCTTCGCCGGGAAGGCTGGCGAGACAACCATAAACGCGTCGAACGCGTGT
ATCGGGAACTGGGGCTTTCGTTGCGCCACAAACGACCTCGACGGAACAAGTCGGCCCGGCGCCGGCAGCCAAAGCAGTCAGTGAGCGCAATCAACGAAAT
CTGGAGTATGGATTTTGTCGCTGACGCGCTGTTTGATGGTCGACGCTTGCGCACGCTCACGATCGTGGACAACTACACGAGAGAATGCCTAGCGATTGAA
GTCGACGGTTCATTGCGTGGCGAGCACGTTGTCGCCGCGCTGACCCGGCTTGCGCAGCATCGTCCGCTTCCCCGCTATATCAAGGCAGACAATGGCAGCG
AGTTCATTTCGAAGACGCTCGACAAATGGGCGTACGAGAACGGCGTGGAGATTGACTTCTCGCGTCCTGGCAAGCCGACCGACAACGCGAAGAATGAATC
ATTCAACGGACGCTTTCGGGAGGAATGCCTCAACGCACATTGGTTCTTGTCGCTCGAGGACGCCAGACGCAAAATCGAGGTCTGGCGCGAGTACTATAAC
GAGGCGCGCCCTCATTCTGCGCTGCAGTGGATGACACCGGCGGAATTCGCCCGCCAGTGCACCGATCGGGCCGATCCGGCCCGCCCCGAAGAGCCGGAAT
TTTCCAACTAAGACCGGGACGGAAATTGGGCCAGCCTCA
CCGAAGAGCAGATCGCATACGCGCTGAAGCAGGCCGAGCTGGGTACGCCTGTCGCGGAGGTCTGCCGCAAGATGGGGATCAGCGACGCGACGTTTTACAA
CTGGCGGACGAAGTACGGCGGGCTGTCACCCTCGGAACTGCGCCGGCTGAAGCAGCTTGAGGAAGAGAACGCGAAGCTCAAGCGGCTCGTGGCCGATCTG
TCGCTGGACAAGGCCATGCTGCAGGATGTGCTGTCAAAAAAGCTCTGAAGCCTTCCCGGCGCCGTGATCTGGTCACGGACTTGATGCAGCGTTTCGGCGC
GAGCCAGCGGCAAACCTGCGCATTGTTGCAGTTGTCCCGCACGGTCTACCGTTACGAATCGGTCGCGCGGGATCAAAGCGCGCTGGAGATGCGCATCAAG
GAGATCACGGAAGTGCGGGTGCATTATGGTGCGCCCCGCGTGTACGTGATGCTTCGCCGGGAAGGCTGGCGAGACAACCATAAACGCGTCGAACGCGTGT
ATCGGGAACTGGGGCTTTCGTTGCGCCACAAACGACCTCGACGGAACAAGTCGGCCCGGCGCCGGCAGCCAAAGCAGTCAGTGAGCGCAATCAACGAAAT
CTGGAGTATGGATTTTGTCGCTGACGCGCTGTTTGATGGTCGACGCTTGCGCACGCTCACGATCGTGGACAACTACACGAGAGAATGCCTAGCGATTGAA
GTCGACGGTTCATTGCGTGGCGAGCACGTTGTCGCCGCGCTGACCCGGCTTGCGCAGCATCGTCCGCTTCCCCGCTATATCAAGGCAGACAATGGCAGCG
AGTTCATTTCGAAGACGCTCGACAAATGGGCGTACGAGAACGGCGTGGAGATTGACTTCTCGCGTCCTGGCAAGCCGACCGACAACGCGAAGAATGAATC
ATTCAACGGACGCTTTCGGGAGGAATGCCTCAACGCACATTGGTTCTTGTCGCTCGAGGACGCCAGACGCAAAATCGAGGTCTGGCGCGAGTACTATAAC
GAGGCGCGCCCTCATTCTGCGCTGCAGTGGATGACACCGGCGGAATTCGCCCGCCAGTGCACCGATCGGGCCGATCCGGCCCGCCCCGAAGAGCCGGAAT
TTTCCAACTAAGACCGGGACGGAAATTGGGCCAGCCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 88 aa | 82 | 345 | + | No |
Description : First part of the transposase
ORF sequence :
MKKSKFTEEQIAYALKQAELGTPVAEVCRKMGISDATFYNWRTKYGGLSPSELRRLKQLEEENAKLKRLVADLSLDKAMLQDVLSKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 316 aa | 261 | 1208 | + | No |
Description : Second part of the transposase
ORF sequence :
GREREAQAARGRSVAGQGHAAGCAVKKALKPSRRRDLVTDLMQRFGASQRQTCALLQLSRTVYRYESVARDQSALEMRIKEITEVRVHYGAPRVYVMLRR
EGWRDNHKRVERVYRELGLSLRHKRPRRNKSARRRQPKQSVSAINEIWSMDFVADALFDGRRLRTLTIVDNYTRECLAIEVDGSLRGEHVVAALTRLAQH
RPLPRYIKADNGSEFISKTLDKWAYENGVEIDFSRPGKPTDNAKNESFNGRFREECLNAHWFLSLEDARRKIEVWREYYNEARPHSALQWMTPAEFARQC
TDRADPARPEEPEFSN
EGWRDNHKRVERVYRELGLSLRHKRPRRNKSARRRQPKQSVSAINEIWSMDFVADALFDGRRLRTLTIVDNYTRECLAIEVDGSLRGEHVVAALTRLAQH
RPLPRYIKADNGSEFISKTLDKWAYENGVEIDFSRPGKPTDNAKNESFNGRFREECLNAHWFLSLEDARRKIEVWREYYNEARPHSALQWMTPAEFARQC
TDRADPARPEEPEFSN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1127 bp | 376 aa | 82 | 1208 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKSKFTEEQIAYALKQAELGTPVAEVCRKMGISDATFYNWRTKYGGLSPSELRRLKQLEEENAKLKRLVADLSLDKAMLQDVLSKKALKPSRRRDLVTD
LMQRFGASQRQTCALLQLSRTVYRYESVARDQSALEMRIKEITEVRVHYGAPRVYVMLRREGWRDNHKRVERVYRELGLSLRHKRPRRNKSARRRQPKQS
VSAINEIWSMDFVADALFDGRRLRTLTIVDNYTRECLAIEVDGSLRGEHVVAALTRLAQHRPLPRYIKADNGSEFISKTLDKWAYENGVEIDFSRPGKPT
DNAKNESFNGRFREECLNAHWFLSLEDARRKIEVWREYYNEARPHSALQWMTPAEFARQCTDRADPARPEEPEFSN
LMQRFGASQRQTCALLQLSRTVYRYESVARDQSALEMRIKEITEVRVHYGAPRVYVMLRREGWRDNHKRVERVYRELGLSLRHKRPRRNKSARRRQPKQS
VSAINEIWSMDFVADALFDGRRLRTLTIVDNYTRECLAIEVDGSLRGEHVVAALTRLAQHRPLPRYIKADNGSEFISKTLDKWAYENGVEIDFSRPGKPT
DNAKNESFNGRFREECLNAHWFLSLEDARRKIEVWREYYNEARPHSALQWMTPAEFARQCTDRADPARPEEPEFSN
Blast result :
Comments
3 copies within J2315. Ends defined by inverted repeats and comparing insertion events.
ISBcen15 is 54% (ORF1) aa similar to ISXcd1 and 76% (ORF2) to ISRso16.
ISBcen15 is 54% (ORF1) aa similar to ISXcd1 and 76% (ORF2) to ISRso16.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth-Smith, H. (2006) Direct submission.
2] Seth-Smith, H. (2006) Direct submission.