ISBcen14

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011001 ND Burkholderia cenocepacia
Burkholderia cenocepacia J2315
DNA section
IS Length : 2516 bp

Ends


IR Length : 18/22

IRL : GTAAGCACGAGACCAGTACCGTCTGTGAACGCTCGGTGACGAGTGCCATG
IRR : GTAAGCGCCCGAGCAGTACCGTAGTGCAGGTTGTTGACCGCGGAGCTCCG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCAAAGGCATCCTGAATCACCCGATCG8
CGTTGACCTCGGTCGGCACGACTTCAGG8
CGGCGGCGCCGGTCATGCCGGCGCCGGA8
AGTAGTGCCGGGACTTGTTCGGCGTCGA8

DNA sequence

GTAAGCACGAGACCAGTACCGTCTGTGAACGCTCGGTGACGAGTGCCATGATGTCCTGTGAAGGACACGAATGACCGGTCGTGTCCACGATAGGAGTTGT
GGACAGAGTGACTCAGACAGTCGAGATCCCGCCGAAACGGCAAACTCGCCGACATCCGACGGAATGGAAGCGGACCATCGTCGCGCTGACATTCGAGCCC
GGCGCGTCGGTCGCCCGTGTTGCTCGCGAGAACGGCATCAATGCCAATCAGGTGTGGGCATGGCGCCGCCTCCATGCGCAAGGCCTGCTAACGGACGATG
CGATTCCAGACGCAATGCTGCCGGTTGTCGTCAACGAACCGTCTCAGCCGTCGATGGCGCTCGAGGTGCCGACTGAGACCGACAACATACCGTCCGGCTC
GATCCAGATTCAGCATGGCAAGACGTCGATTCGCATCGAAGGCGCGCCGGACCCCGACGTGCTGCGTTCTGTTCTCGATCGCATTCTACGATGATCGGTC
TGCCTCAAAACACGCGGATCTGGATCGCGGCGGGCGTCACCGATATGCGATGCGGCTTCAATTCGTTGGCCGCGAAGGTACAGACGGTGCTGGAGAAAGA
TCCGTTCTCTGGACACGTGTTCGTGTTCCGGGGCAAGCGCGGTGACCTGCTGAAGTGCTTGTATTGGAGCGACGGCGGGCTATGTTTATTGGCGAAGCGG
CTCGAAAAAGGACGCTTTGCTTGGCCCCGTGCCGACTCTGGTGTGGTCGCGCTGACAACCGCGCAGCTGTCGTTGTTGCTCGAGGGCTTCGATTGGCGAC
AGCCGGTCGAAGCCGTGCGTCCACGTAGCGCGCTGTAAACCATTGTTAACTGGCGTTCGCGTGATCGCGGGCAGTCGTAAACTGTCCTCATGGAATCAAC
CTCGACCGCGCTGCCCGACGACATCAACGCTCTGAGAGCGTTGCTGCTTGAGCGTGATGCTCAGGTAGCTGAGCTGCGAAAGCAGCTCTCGTCGCGGGCT
CTCGAAATCGAGCATCTGAAGCTCACGATCGCGAAACTGCGCCGAATGCAGTTCGGCCGGAAATCGGAGAAACTAGATCTCCAGATCGAACAGCTCGAAT
TGCGGCTGGAGGATCTGCAGGCTGACGAAGGCGCCGCCGATGCGTCTGCCGCTCCCGAGGCAAAGCGGCCGCGCCGCGAAGGTGCGAGCCGCAAGCCGCT
ACCCGGACATCTTGAACGCGAAGAGCGCGTCCATCTGCCCGCCGATGATGACTGCCCCGATTGCGGTGGGCAGCTCAAGCCGTTGGGCGAAGACATCGCG
GAGCAACTCGAATACGTGCGTGCGCACTTCCGTGTGATCCGTCATCGCCGCCCGAAGCTGGCCTGCGCGCGCTGTGACCGCATCGTGCAGGCCGCGGCGC
CGAGCCGCCCAATCGATCGGGGCATCCCGGGTCCGGCGCTGCTCGCCCACATCGCCGTGTCGAAGTTCGCATACCACCTCCCGCTGCACCGCCAGGCGGT
GATGTACGCGCGTGACGGCGTCGAGATCGATCCCGGCGCGATGGGCTACTGGATGGGTAGCATCACGGCGCTGCTCGCACCGTTGGTCGACGCCGTGCGC
CGCTACACGTTGGCCTGCGGTAAGGTGCATGCGGACGACACTCCGCTACCGGTGCTGGTGCCGGGCAACGGTCGTACGAAGACAGGACGCCTCTGGGTCT
ACGTGCGCGATGATCGGCAGAGTGGCTCCGACGAACCGCCGGCGGCCTGGTTCGCCTACACGCCTGATCGCCGTGGTGAGCATCCGCAGCGACATCTCGC
TGACTTCGCCGGCGTGCTGCAGGCTGACGCGTTCGCTGGCTATGCCGAGCTGTATCTCGACGGCCGCGTCCAGGAAGCCGCATGTATGGCTCACGCGCGC
CGGAAGATCCACGACCTGCATGCGGTGCGCCCCAACGCCGTGACGGAGGAGGCGCTGCGGCGGATCGGTGCGCTCTACAAGATCGAAGAGCAGATCCGCG
GCAAGCCGCCCGACGAACGGCGCAGTGTGCGCCAAGCGCGGGCGGTGCCGCTACTCGACGACATGAAGCGCTGGTTCGAAGCGACGCTTGCCACGCTCTC
TGCAAAATCTGACACGACCAAGGCGATTCGGTACGCGCTGAATCGCTGGCCGGCGCTCGTCTACTACTGCAGCGATGGGTGTACGGAGATCGACAACCTG
ATCGCCGAGCGAGCATTGCGAGGCGTCGCTCTCGGACGGCGGAACTATCTGTTCGCCGGCGCCGACTCTGGCGGCGAACGTGCCGCCGCGATGTACAGCC
TGATCGGCACGGCACGTCTGAACGGCCTCGATCCCGAGGCGTATCTCGCCTACGTGCTCGAGCGCATCGCCGACCATCCGGCCAACCGGGTCGACGAACT
GCTCCCGTGGAACGTCGCACCATCGCTGCCGCCTACTGCTCGCGTCGAGCCCATCCGATAGCGTCGCGGAGCTCCGCGGTCAACAACCTGCACTACGGTA
CTGCTCGGGCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
396 bp131 aa99494+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

VDRVTQTVEIPPKRQTRRHPTEWKRTIVALTFEPGASVARVARENGINANQVWAWRRLHAQGLLTDDAIPDAMLPVVVNEPSQPSMALEVPTETDNIPSG
SIQIQHGKTSIRIEGAPDPDVLRSVLDRILR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa491838+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIGLPQNTRIWIAAGVTDMRCGFNSLAAKVQTVLEKDPFSGHVFVFRGKRGDLLKCLYWSDGGLCLLAKRLEKGRFAWPRADSGVVALTTAQLSLLLEGF
DWRQPVEAVRPRSAL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1572 bp523 aa8902461+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MESTSTALPDDINALRALLLERDAQVAELRKQLSSRALEIEHLKLTIAKLRRMQFGRKSEKLDLQIEQLELRLEDLQADEGAADASAAPEAKRPRREGAS
RKPLPGHLEREERVHLPADDDCPDCGGQLKPLGEDIAEQLEYVRAHFRVIRHRRPKLACARCDRIVQAAAPSRPIDRGIPGPALLAHIAVSKFAYHLPLH
RQAVMYARDGVEIDPGAMGYWMGSITALLAPLVDAVRRYTLACGKVHADDTPLPVLVPGNGRTKTGRLWVYVRDDRQSGSDEPPAAWFAYTPDRRGEHPQ
RHLADFAGVLQADAFAGYAELYLDGRVQEAACMAHARRKIHDLHAVRPNAVTEEALRRIGALYKIEEQIRGKPPDERRSVRQARAVPLLDDMKRWFEATL
ATLSAKSDTTKAIRYALNRWPALVYYCSDGCTEIDNLIAERALRGVALGRRNYLFAGADSGGERAAAMYSLIGTARLNGLDPEAYLAYVLERIADHPANR
VDELLPWNVAPSLPPTARVEPIR

 

Blast result :
Comments
4 copies within J2315. Ends defined by inverted repeats, direct repeats and comparing multiple insertion events.
ISBcen14 is 58% (ORF1), 80% (ORF2) and 71% (ORF3) aa simialr to IS883.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth-Smith, H. (2006) Direct submission.