ISBcen9

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Burkholderia cenocepacia
Burkholderia cenocepacia J2315
DNA section
IS Length : 1439 bp

Ends


IR Length : 22/25

IRL : TGAACTGAACCCCAAAAGTTGGACGCCAACTTTTGGGTTTTTTTCATGGC
IRR : TGAACTGAACCCACGAAGTTGGACGGTTACCGGCTAGGCCGGATGAGGCT

Insertion site


Left flankDirect repeatRight flankDR Length
CACCGGCCTGTTGCGGCCCGGTG3
GACCGCGCGCTTTAAGAGCGGCG3
GCCGCGCCCCGAAAGGGACGCGGC4
GCCGCCGTGCGCAAGCACGGCGGC4
GCCGCCGTTGCTTGCGCACGGCG3
GCCGTCACGGTTTCCCGTGACGGC4
CACCGCGCGCGATGCGCCGGTGGC4
CCGCCGGTGGAAGCCGGCGGGTT3
GCCGCCGCATGCAAATGCGGCGGC4

DNA sequence

TGAACTGAACCCCAAAAGTTGGACGCCAACTTTTGGGTTTTTTTCATGGCGAGATATACCGAGCAGTTCAAGCTATCGGTAGTGAAGCGGTGCATCGGGG
GGGAAGCCATACGCGCGGTGGCGCGAACGCATGGCTTGAGTCACTCGACGGTTTCCCAGTGGTACGCCACATACCAGGCTCATGGTAAGGACGGCGTGCG
TAGGAAGTACAACTCATACGATGTGGCGTTCAGGCTCAAAGTTGTTCAGCACATGCGCGAGCATGGCGTGTCGAGCAAGGAAGCGGCGGCGCGGTTCAAT
ATTCGCAATCCGAGCGCGGTGCTCGAATGGGCTCGCCGCTATGATGATGGTGGTTTGACTGCCCTCGCGCCGAGGCCCAAAGGCCGCCGCCCAACCGCAA
TGCCAAAAACACCGCCTGCTCAGCCTCTGAACCCTACCGATGGCACGGATACCCGTACCCGCGAGGACCTGCTTCAGGAACTGAACTACCTGCGCATGGA
GAACGCGTATCTAAAAAAGCTGAGGGCCTTGGTTCAGGCGCAGGCTGTACCGCGCAAAAAGCGCAAATAGTGCTTGAGCTAAGGCAACAATTCCCGCTGG
CTGGCTTGCTCCGGGTGGCCGGACTGGCTCGCAGCACGTTTTATTACCAGTGCAAGGCGCTGGCCGCTCCCGATCGACATGCGAGTGTGAAAGCGAAAAT
TCGAGCACTGTTCGAGCAGCACAAGGGGCGCTATGGCTATCGACGCATCACGCTGGCGCTGCGCCGTCTGGGGCAGATGATTAACCACAAGACGGTTGCA
CGCCTGATGCGCAAGATGCAGTTGAAGTCCTGCGTGCGGGTCAAGAAGTACCGTGCGTATAGGGGCAACACGTGCAAGACGGCCCCGCATCTGCTCCAGC
GCCAGTTCCATGCCGCCCGTCCGAACGAGAAGTGGGTTACCGACATCACCGAGTTCAGCGTAGGTGGCCAAAAGCTGTATCTGTCACCAGTGCTGGATCT
GTACAACGGGGAGATCATCGCGTACCAGACGCATACTCGGCCAGCCTTCCAGATGGTTACTGACATGTTGCGCAAGGCTTTGCGTCGCCTGCAACCCGAC
GAAAGACCCATGTTGCACTCTGATCAAGGCTGGCATTATCAAATGCCCGGCTGGCGGCTCATGCTCGAGCAACGCTCGTTGGCGCAAAGCATGTCGCGTA
AAGGCAATTGCTTGGACAACGCTGCCATGGAAAGCTTCTTCGGCACGCTGAAATCCGAGTTCTTCTACTTGAACCACTTCAGCAGCATTGAACAGCTACG
CATCGGCTTGAAGCGCTACATCCGGTACTACAATCACGAGCGCATCAAGCTCAAACTGAAAGGGCTGAGTCCTGTGCAATACAGGACTCAGCCTCATCCG
GCCTAGCCGGTAACCGTCCAACTTCGTGGGTTCAGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
522 bp174 aa46567+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MARYTEQFKLSVVKRCIGGEAIRAVARTHGLSHSTVSQWYATYQAHGKDGVRRKYNSYDVAFRLKVVQHMREHGVSSKEAAARFNIRNPSAVLEWARRYD
DGGLTALAPRPKGRRPTAMPKTPPAQPLNPTDGTDTRTREDLLQELNYLRMENAYLKKLRALVQAQAVPRKKRK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1056 bp352 aa3481403+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

WWFDCPRAEAQRPPPNRNAKNTACSASEPYRWHGYPYPRGPASGTELPAHGERVSKKAEGLGSGAGCTAQKAQIVLELRQQFPLAGLLRVAGLARSTFYY
QCKALAAPDRHASVKAKIRALFEQHKGRYGYRRITLALRRLGQMINHKTVARLMRKMQLKSCVRVKKYRAYRGNTCKTAPHLLQRQFHAARPNEKWVTDI
TEFSVGGQKLYLSPVLDLYNGEIIAYQTHTRPAFQMVTDMLRKALRRLQPDERPMLHSDQGWHYQMPGWRLMLEQRSLAQSMSRKGNCLDNAAMESFFGT
LKSEFFYLNHFSSIEQLRIGLKRYIRYYNHERIKLKLKGLSPVQYRTQPHPA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1348 bp453 aa561403+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARYTEQFKLSVVKRCIGGEAIRAVARTHGLSHSTVSQWYATYQAHGKDGVRRKYNSYDVAFRLKVVQHMREHGVSSKEAAARFNIRNPSAVLEWARRYD
DGGLTALAPRPKGRRPTAMPKTPPAQPLNPTDGTDTRTREDLLQELNYLRMENAYLKKAEGLGSGAGCTAQKAQIVLELRQQFPLAGLLRVAGLARSTFY
YQCKALAAPDRHASVKAKIRALFEQHKGRYGYRRITLALRRLGQMINHKTVARLMRKMQLKSCVRVKKYRAYRGNTCKTAPHLLQRQFHAARPNEKWVTD
ITEFSVGGQKLYLSPVLDLYNGEIIAYQTHTRPAFQMVTDMLRKALRRLQPDERPMLHSDQGWHYQMPGWRLMLEQRSLAQSMSRKGNCLDNAAMESFFG
TLKSEFFYLNHFSSIEQLRIGLKRYIRYYNHERIKLKLKGLSPVQYRTQPHPA

 

Blast result :
Comments
9 copies within J2315.
ISBcen9 is 61% (ORF1) aa similar to IS1221A and 79% (ORF2) aa similar to ISRso11.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth-Smith, H. (2006) Direct submission