ISBcen7

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Burkholderia cenocepacia
Burkholderia cenocepacia J2315
DNA section
IS Length : 1312 bp

Ends


IR Length : 20/25

IRL : TGACCCATCCTGAAATAGGTTGACACTTTTTCGCCGAGACGAAGAGGTGG
IRR : TGTCAAGTCCTGGAATAGGTTGACAGTATCCAGACGCTAGAACGCCCGAT

Insertion site


Left flankDirect repeatRight flankDR Length
TTTTGTTTTTTGTGTCTCGCCCC3
TTTTTTCGTCTGTTGAACACGAT3

DNA sequence

TGACCCATCCTGAAATAGGTTGACACTTTTTCGCCGAGACGAAGAGGTGGCAAGTGGAGCAAGGGATCAAGCGGACGCAGCGAGATTACAGCCTGGCTTT
TAAGCTGTCAGTGGTGGCGCAGGTCGAAAAAGGCGAACTGACGTACAAAGAGGCGCAGCGGCGCTACGGCATCCAGGGTCGCTCGACGGTGCTGGTATGG
TTACGCAGGCACGGCTTGCAGGATTGGGCCGATCCATCCGGCCGTGCGCAATCGGGTTCCACCATGCCAAAGCCAGACTCAAAGCCGCTCACACCCGAGC
AGCGCATCAAGGAGTTGGAGACGCAGTTGCGAGAGGCGCAGGAGAAAGCGGCGCTATTCGAGGCGGTGCTCGACGTGATGCGCAAGGACTACGGGATCAC
CGTAAAAAAGCCTTCGGGCAGGTCGTCGCGCAAAAGCGGATCGAAAAGCTGAGCGTCGAGCGAGCCTGCCGCTACATGGGCATCAGCCGCCAAGCGTATT
ACAAGCGATGTCGCAGCGAGCAGTGCCGTTGCTCGCAAGCTGAGACCGTGGCGACGCTGGTGCGGGACGTCCGGCTTCGCCAGCCGAGGCTCGGAACACG
AAAGCTGCATCATTTGCTCGGGCCTGCATTGGTGGAGCGTGGGATCAAGCTGGGACGGGATCGGCTGTTCGACGTGCTGCGCGCCGCGCGCCTGCTGGTC
GTGCCGCACCGGGCGTACCACAAGACGACTCAAAGCCATCATCGCTTCCGGCGTCATCCGAATCTGCTGAAACCCGGGCCGGATCAAATCGTGCCGACCG
GCCCCGAACAGGTATGGGTGGCCGACATCACGTACCTGCCGACGGCTGGCCCGTTTGTGTATTTGAGCCTCGTCACGGATGCGTTCTCGCGCAAGATCGT
CGGCCATCACGTTCACGAGAGCCTGCAAACCGAGCAGGTCAGTCAGGCGCTGAAGAAGGCGTTGCGCAGGCGCCAGACCCGCCAGCCCTTGATCCATCAC
TCCGATCGCGGGGTCCAGTACTGCGCGACCTACTACCAGGACATCCATCGGCGTCACGGCTTGCGATGTTCGATGACGGACGGCTACGACTGCTATCAGA
ACGCGCTGGCCGAGCGGGTCAACGGCATTCTCAAGGGCGAGTTCCTGCTGCAACGTCCCGCTGACCTCGAGCAGGCCGCCAAGATGGTCGAACAGTCCAT
CCGCATCTATAACCACGAGCGACCGCATGCTGCGCTGAAATACAAAACGCCCGATGCGGTGCATCGGGCGTTCTAGCGTCTGGATACTGTCAACCTATTC
CAGGACTTGACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
396 bp132 aa54449+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MEQGIKRTQRDYSLAFKLSVVAQVEKGELTYKEAQRRYGIQGRSTVLVWLRRHGLQDWADPSGRAQSGSTMPKPDSKPLTPEQRIKELETQLREAQEKAA
LFEAVLDVMRKDYGITVKKPSGRSSRKSGSKS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1170 bp388 aa1041273+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AVSGGAGRKRRTDVQRGAAALRHPGLDGAGMVTQARLAGLGRSIRPCAIGFHHAKARLKAAHTRAAHQGVGDAVARGAGESGAIRGGARRDAQGLRDHRK
KAFGVVAQKRIEKLSVERACRYMGISRQAYYKRCRSEQCRCSQAETVATLVRDVRLRQPRLGTRKLHHLLGPALVERGIKLGRDRLFDVLRAARLLVVPH
RAYHKTTQSHHRFRRHPNLLKPGPDQIVPTGPEQVWVADITYLPTAGPFVYLSLVTDAFSRKIVGHHVHESLQTEQVSQALKKALRRRQTRQPLIHHSDR
GVQYCATYYQDIHRRHGLRCSMTDGYDCYQNALAERVNGILKGEFLLQRPADLEQAAKMVEQSIRIYNHERPHAALKYKTPDAVHRAF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1220 bp406 aa541273+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEQGIKRTQRDYSLAFKLSVVAQVEKGELTYKEAQRRYGIQGRSTVLVWLRRHGLQDWADPSGRAQSGSTMPKPDSKPLTPEQRIKELETQLREAQEKAA
LFEAVLDVMRKDYGITVKKAFGVVAQKRIEKLSVERACRYMGISRQAYYKRCRSEQCRCSQAETVATLVRDVRLRQPRLGTRKLHHLLGPALVERGIKLG
RDRLFDVLRAARLLVVPHRAYHKTTQSHHRFRRHPNLLKPGPDQIVPTGPEQVWVADITYLPTAGPFVYLSLVTDAFSRKIVGHHVHESLQTEQVSQALK
KALRRRQTRQPLIHHSDRGVQYCATYYQDIHRRHGLRCSMTDGYDCYQNALAERVNGILKGEFLLQRPADLEQAAKMVEQSIRIYNHERPHAALKYKTPD
AVHRAF

 

Blast result :
Comments
ISBcen7 is 79% (ORF1) and 77% (ORF2) aa similar to ISPsy11.
2 copies within J2315. Ends defined by inverted repeats, direct repeats and comparing insertion events.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Helena Seth-Smith (2006) Direct submission