ISBcen6
- Family IS3
- Group IS2
Isoform Synonym(s) ISBmu32
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 Burkholderia multivorans ATCC 17616 |
DNA section
IS Length : 1335 bp
Ends
IR Length : 31/38
IRL : TGGAGCAGCCCCTGAAACACCGGACATAGCCTCTCACTTAAACTAGCGGG
IRR : TGGAGTTGACCCTGTAATACCGGACACAGCCTCACACTAAGGTTGCTGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGCACTGCC | GATACATACT | 0 |
DNA sequence
TGGAGCAGCCCCTGAAACACCGGACATAGCCTCTCACTTAAACTAGCGGGTAGGCATAAGACTGTGTTTTTGACTAACACCAGGCAGGAAGTGATGGAAG
TGTTGACGGGCCCTGAGCGTCGGCGGCGCTGGACGGCGGAGCAGAAGCTGGCGATGGTTCGCGAGAGTTTCGAGCCGGGGAAATCGGTTTCGATGGTCGC
GCGCCAGCACGGCGTGAATCCGAACCAACTGTTCCACTGGCGCAAGCTGTACCAGGATGGGAGCCTGTCAGCGGTCAAGGCCGGCGAGGAAGTAGTCCCG
GCCTCGGAACTGGCCGATGCACTCAAGCAGATTCGTGAGCTGCAACGGATGCTCGGCAAAAAGACCATGGAGCACGAGATTCTCCGCGAAGCAGTCGAGT
ACGGCCGGGCAAAAAAATGGATAGCGCACTCGCCCTCGCTGCCGGAGGACGACCAGTGAAACTGGTTTGTGAAGTTCTCGGCGTGTCGCGCTCGAACGTA
TCGGCACGACTGTCGCGTCCGGCGACGTGGCGCGATGGCCGTCAATCGAGGCAGAGTGACGACGCGGCCGCGGTCGAGGAGATCCGCCGGGTCATTGGCG
ATTTGCCCAGCTATGGCTATCGCCGGGTTTGGGGCATCTTGCGTAACGAGCGCGTTGCGGTCGGACTGGCGCCGTTTAATGCCAAGCGAATCTATCGCGT
CATGCGCACACATGGGCTGCTGATGCAGCGCCGACCGATTCCACCTCGGCCCCAACGTCGACACGACGGCAAGGTAGCCGTCGAGCGCAGCAATCAGCGA
TGGTGCTCGGACGGCTTCGAGTTCCGCTGCGACAACGGCGAGCCGCTGCGCGTAACGTTCGCGCTGGATTGCTGTGACCGTGAAGCGATGAGCTGGGCGG
CTACGACGGCAGGCCACAGCGGCGACATCGTGCGCGACGTGATGCTGGCTGCAGTGGAAAATCGGTTTGGCATCGAGCTGCATACGCCGTCCGAAATCGA
GTGGCTGAGCGACAACGGTTCGGGCTACACGGCCGACGACACGCGTCGGTTCGCAGTGGCCATCGGCCTGAAGCCATTGACCACGCCGGTATGCAGCCCG
CAAAGTAACGGGATGGCCGAGAGCTTCGTGAAGACGATGAAACGCGACTACGTCGCCTTCATGCCGAAGCCGGACGCAGCGACTGCTGCACGCAACTTGG
CCATCGCGTTCGAGCATTACAACGAGAAGCGTCCCCATAGCGCGCTGAAATACCGCTCGCCTCGCGAGTTCCGGCGCTCGATGGATTCAGCAACCTTAGT
GTGAGGCTGTGTCCGGTATTACAGGGTCAACTCCA
TGTTGACGGGCCCTGAGCGTCGGCGGCGCTGGACGGCGGAGCAGAAGCTGGCGATGGTTCGCGAGAGTTTCGAGCCGGGGAAATCGGTTTCGATGGTCGC
GCGCCAGCACGGCGTGAATCCGAACCAACTGTTCCACTGGCGCAAGCTGTACCAGGATGGGAGCCTGTCAGCGGTCAAGGCCGGCGAGGAAGTAGTCCCG
GCCTCGGAACTGGCCGATGCACTCAAGCAGATTCGTGAGCTGCAACGGATGCTCGGCAAAAAGACCATGGAGCACGAGATTCTCCGCGAAGCAGTCGAGT
ACGGCCGGGCAAAAAAATGGATAGCGCACTCGCCCTCGCTGCCGGAGGACGACCAGTGAAACTGGTTTGTGAAGTTCTCGGCGTGTCGCGCTCGAACGTA
TCGGCACGACTGTCGCGTCCGGCGACGTGGCGCGATGGCCGTCAATCGAGGCAGAGTGACGACGCGGCCGCGGTCGAGGAGATCCGCCGGGTCATTGGCG
ATTTGCCCAGCTATGGCTATCGCCGGGTTTGGGGCATCTTGCGTAACGAGCGCGTTGCGGTCGGACTGGCGCCGTTTAATGCCAAGCGAATCTATCGCGT
CATGCGCACACATGGGCTGCTGATGCAGCGCCGACCGATTCCACCTCGGCCCCAACGTCGACACGACGGCAAGGTAGCCGTCGAGCGCAGCAATCAGCGA
TGGTGCTCGGACGGCTTCGAGTTCCGCTGCGACAACGGCGAGCCGCTGCGCGTAACGTTCGCGCTGGATTGCTGTGACCGTGAAGCGATGAGCTGGGCGG
CTACGACGGCAGGCCACAGCGGCGACATCGTGCGCGACGTGATGCTGGCTGCAGTGGAAAATCGGTTTGGCATCGAGCTGCATACGCCGTCCGAAATCGA
GTGGCTGAGCGACAACGGTTCGGGCTACACGGCCGACGACACGCGTCGGTTCGCAGTGGCCATCGGCCTGAAGCCATTGACCACGCCGGTATGCAGCCCG
CAAAGTAACGGGATGGCCGAGAGCTTCGTGAAGACGATGAAACGCGACTACGTCGCCTTCATGCCGAAGCCGGACGCAGCGACTGCTGCACGCAACTTGG
CCATCGCGTTCGAGCATTACAACGAGAAGCGTCCCCATAGCGCGCTGAAATACCGCTCGCCTCGCGAGTTCCGGCGCTCGATGGATTCAGCAACCTTAGT
GTGAGGCTGTGTCCGGTATTACAGGGTCAACTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 364 bp | 121 aa | 94 | 457 | + | No |
Description : First part of the transposase
ORF sequence :
MEVLTGPERRRRWTAEQKLAMVRESFEPGKSVSMVARQHGVNPNQLFHWRKLYQDGSLSAVKAGEEVVPASELADALKQIRELQRMLGKKTMEHEILREA
VEYGRAKKWIAHSPSLPEDDQ
VEYGRAKKWIAHSPSLPEDDQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 321 aa | 339 | 1301 | + | No |
Description : Second part of the transposase
ORF sequence :
AATDARQKDHGARDSPRSSRVRPGKKMDSALALAAGGRPVKLVCEVLGVSRSNVSARLSRPATWRDGRQSRQSDDAAAVEEIRRVIGDLPSYGYRRVWGI
LRNERVAVGLAPFNAKRIYRVMRTHGLLMQRRPIPPRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRD
VMLAAVENRFGIELHTPSEIEWLSDNGSGYTADDTRRFAVAIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPDAATAARNLAIAFEHYNEKRPH
SALKYRSPREFRRSMDSATLV
LRNERVAVGLAPFNAKRIYRVMRTHGLLMQRRPIPPRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRD
VMLAAVENRFGIELHTPSEIEWLSDNGSGYTADDTRRFAVAIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPDAATAARNLAIAFEHYNEKRPH
SALKYRSPREFRRSMDSATLV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1208 bp | 403 aa | 94 | 1301 | + | Yes |
Chemistry : DDE
ORF sequence :
MEVLTGPERRRRWTAEQKLAMVRESFEPGKSVSMVARQHGVNPNQLFHWRKLYQDGSLSAVKAGEEVVPASELADALKQIRELQRMLGKKTMEHEILREA
VEYGRAKKMDSALALAAGGRPVKLVCEVLGVSRSNVSARLSRPATWRDGRQSRQSDDAAAVEEIRRVIGDLPSYGYRRVWGILRNERVAVGLAPFNAKRI
YRVMRTHGLLMQRRPIPPRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRDVMLAAVENRFGIELHTPS
EIEWLSDNGSGYTADDTRRFAVAIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPDAATAARNLAIAFEHYNEKRPHSALKYRSPREFRRSMDSA
TLV
VEYGRAKKMDSALALAAGGRPVKLVCEVLGVSRSNVSARLSRPATWRDGRQSRQSDDAAAVEEIRRVIGDLPSYGYRRVWGILRNERVAVGLAPFNAKRI
YRVMRTHGLLMQRRPIPPRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRDVMLAAVENRFGIELHTPS
EIEWLSDNGSGYTADDTRRFAVAIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPDAATAARNLAIAFEHYNEKRPHSALKYRSPREFRRSMDSA
TLV
Blast result :
Comments
ISBcen6 is 97% (ORF1) and 90%(ORF2) aa similar to ISBugl1.
1 full copy and 1 partial copy within J2315. Full copy interrupted by ISBcen4 element.
ISBmu32 is a partial copy of ISBcen6.
1 full copy and 1 partial copy within J2315. Full copy interrupted by ISBcen4 element.
ISBmu32 is a partial copy of ISBcen6.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Helena Seth-Smith (2006) Direct submission.
2] Helena Seth-Smith (2006) Direct submission.