ISH22

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_002607 ND Halobacterium sp.
Halobacterium sp. NRC-1
DNA section
IS Length : 1691 bp

Ends


Left end : AGCGGTAGCAAGGCGAAAGCCCACCCGTTTACGGGTGGGATGAAGCCGACAACTGGGAATCTATACACGCCCGTAGCCACGGCTGGGGTTGGATGTTTGC II struct. : Yes
Right end : TCATCGTCCACCGTGACGACGAACCTGCGAACCTCTCCGTTTCCGTGGATTCAACCCCCAGTGGGGGAACCCCACGGCTTTAGCCGTGGGAGGATGTCAG II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TCCGACACAGTGACACGCGGATGTTCAG

DNA sequence

AGCGGTAGCAAGGCGAAAGCCCACCCGTTTACGGGTGGGATGAAGCCGACAACTGGGAATCTATACACGCCCGTAGCCACGGCTGGGGTTGGATGTTTGC
GTAATATCTTTAGGTGAAGCTATTCTATATACTGACGATGCCTCGTGGGTTCGACAGGGAACGTACCAACGTCCATAAACTCCAGTACCACTTCATCTGG
TGTCCGAAGTACCGCAAATCGGTACTAACGGGCGAGGTACGCGACCGGCTCGAAGAACTCATCAAGGAGAAAGCCGACGAACTCGGTGTAGAGATACTGG
AGTTGGCGATTCGACCCGACCACGTACACCTGTTCATCACGGGCGATCCAACGCTCGCTCCGAACAAGATCATGCAACAGATCAAGGGCTACTCCTCGCG
CCACCTGCGTGATGAGTTCGACTTCGGCTTGCCGTCACTGTGGACGCGCTCGTATTTCGTATCAAGTGCGGGTGACGTGTCCAGCGAGGTCATCGAGGAG
TACATCGACGCCCAAGCAGGTGAGTAGTATGCAGCGGAACGTCTCAACCACGGTGCGGGTCAAACTCCACTCGCTGACCAACAGGAAAGCCGACCTTCTC
GCCCGCGAGTACGAGGCGTTCCAGACTGAGGTTCACGACGGAGACGCCGACCTCTACTCTGCGACCGACCAGCAGGCGTCGAAAGTTCAGCGACAAAAAG
ACCCGAATCCCGGCACTGAACAGCCTGTCGTTCTCCGCAACGACGTGTTCGACGTGTCTCACGACGAGGACACTGTTCTCTCGTCGTGGTGGGTCAACGT
CCCCGTCTACGATCCCGAACGGGGACGGGGGAACTCAATTTGGTGTCCTGCCCACATCCCCCGGAAGGACGAACAACTCGTCCGTGAGGGCGATGTTCGG
GACAGCGAGTTAGTGCGCCGTGACGGTGACTGGTATGTCCATCTCGTCGTCAAACGGTCTGTGACCGTGGCCGACGAGTACGACGACGTACTGGCTATCG
ACATGGGCGCACGATGGGTCGCCACCTGCGCGTTCCTCTCCGACCGCACAACCACGTTCTACGGCGAGGAAGTTCGCCGCATCCGCGAACACTACAAACA
ACTGCGGAAGTCTATCGGGAAGGCGAAACCCCGACAAGGACAGCAGGTGGTCGAGCGTATCGGTGACGCCGAGGCACGGAAAGTGGACGACCGGCTCCAC
AAGATTGCTCGGCAAATCGTAGAGGATGCCGAGGAGCGGAACGCGGTTATCGTCGTGGGCGACCTCGGTGGGATTCGCAAGGACAACGACAAGGGGCGGT
ACGTCAACGACAAGACCCACAAGATGCCGTTCGCCCGCCTGCTCAACTGTATCGAGTACAAGGCCCACGACGCGGGTATCGACGTGCAGCTGGTCAACGA
GTACGACACGTCGAAGACGTGCAACCGCTGTGCCTGCGAGGGAGTCCGAGCAACGCAGGGACGCTTCGAGTGTCCCGAGTGCGGACTGGATGATAACGCA
GACAAGAACGGTGCGCTGAACATCGGCAAACGAGCCTTGGGCAAGTTCTCGAAACCGCTGTCCGAGGCGGGGGCTGTACTGGCACAGCCCGAAACGCAGG
TCATCGTCCACCGTGACGACGAACCTGCGAACCTCTCCGTTTCCGTGGATTCAACCCCCAGTGGGGGAACCCCACGGCTTTAGCCGTGGGAGGATGTCAG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
390 bp129 aa138527+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MPRGFDRERTNVHKLQYHFIWCPKYRKSVLTGEVRDRLEELIKEKADELGVEILELAIRPDHVHLFITGDPTLAPNKIMQQIKGYSSRHLRDEFDFGLPS
LWTRSYFVSSAGDVSSEVIEEYIDAQAGE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1155 bp384 aa5291683+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MQRNVSTTVRVKLHSLTNRKADLLAREYEAFQTEVHDGDADLYSATDQQASKVQRQKDPNPGTEQPVVLRNDVFDVSHDEDTVLSSWWVNVPVYDPERGR
GNSIWCPAHIPRKDEQLVREGDVRDSELVRRDGDWYVHLVVKRSVTVADEYDDVLAIDMGARWVATCAFLSDRTTTFYGEEVRRIREHYKQLRKSIGKAK
PRQGQQVVERIGDAEARKVDDRLHKIARQIVEDAEERNAVIVVGDLGGIRKDNDKGRYVNDKTHKMPFARLLNCIEYKAHDAGIDVQLVNEYDTSKTCNR
CACEGVRATQGRFECPECGLDDNADKNGALNIGKRALGKFSKPLSEAGAVLAQPETQVIVHRDDEPANLSVSVDSTPSGGTPRL

 

Blast result :
Comments
ISH22 is 66% (ORF A) aa similar to ISTac1 and 44% (ORF B) aa similar to ISH12.

File updated on February 13 2015 : modification of the ends accordingly with the IS200/IS605 family rules.
References
1]Ng,W.V., Kennedy,S.P., Mahairas,G.G., Berquist,B., Pan,M., Shukla,H.D., Lasky,S.R., Baliga,N., Thorsson,V., Sbrogna,J., Swartzell,S., Weir,D., Hall,J., Dahl,T.A., Welti,R., Goo,Y.A., Leithauser,B., Keller,K., Cruz,R., Danson,M.J., Hough,D.W., Maddocks,D.G., Jablonski,P.E., Krebs,M.P., Angevine,C.M., Dale,H., Isenbarger,T.A., Peck,R.F., Pohlschrod,M., Spudich,J.L., Jung,K.-H., Alam,M., Freitas,T., Hou,S., Daniels,C.J., Dennis,P.P., Omer,A.D., Ebhardt,H., Lowe,T.M., Liang,P., Riley,M., Hood,L. and DasSarma,S.(2000) Proc. Natl. Acad. Sci. U.S.A. 97 (22), 12176-12181