ISLpl4
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ968657 | ND | Lactobacillus plantarum | Oenococcus oeni Lactobacillus plantarum CECT 4645 Lactobacillus plantarum Leuconostoc mesenteroides Lactobacillus sakei |
DNA section
IS Length : 985 bp
Ends
IR Length : 29/35
IRL : ACGCGTGGTGCTAGTTTGTAATAATACAAAAAAATTAACAAAAAAGTCCA
IRR : ACGCGTGGTGCTAGTTCGTCACAAAATTAAAAAATCCTAAATTATAGACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATAT | GCTAACAT | AATTAC | 8 |
| CTTTAT | GCTCTAGT | ATTAAG | 8 |
| TACTAT | GTGTTAAC | TAAAAA | 8 |
DNA sequence
ACGCGTGGTGCTAGTTTGTAATAATACAAAAAAATTAACAAAAAAGTCCAACACAGTTAGACTCATAAGTGACTAAACTTAAAAGGAGTCCTAACGATGT
TGAACCATCCTAAGTTTAAACAAATTCGTCACGAATTACAAGGTAACTATCATTATTTTTATCAGCTGTGCCAGTCGCTTTACCAGCGCTATTGCCCACG
TTGTGTGACGCAACGGCGTAATGTGGCGCACGTTAAAATCGATGACTGTCGATTGTTGGCGCTACTGTGTTTACAAACAACGCTTGGTATCCAATCACAA
CGCCGTTTTTGGCGCTTGATGACCGCCTTTATGCCGCAAAAAATCACCATTAGTCGGTCACGGTTCAACCGTCGCGCACTACAATTATTACCCGTGGTCA
ATGCGATCCGTACTGGTTTGACGCGTGAAGCAGCCCAACCTGGACAGATCGCAATCATTGATAGTTTGCCGAATCCATTGTGTGAACCAGTACGTAAATT
TAGAGCACGTATTTTTGCTGGGCAAGCTAATATTGGCTACAATGCCACTAAGCAAATGTCGTTCTATGGGTTTAAAACCCACATGCTAGTAACCACTGAT
GGCTACATTCTGAATTATGTGGTGACTGCCGCTTCGGTCCATGATACAAAAGTTGCCGTTAGCTTGATTGATGATTGTCCTTGTCCAATTGTTTTGGCCG
ATGTCGGTTATGTAGGCAAGCGACTTGGCGCTGAATTTAAGCAATTAGGCTACACGTTATGGACACCATACCGTTCGAATATGAAGGGGGCCAAGGAACA
TAATAAGCGCGCACTCAAGGCGTTACGTCGCACAATTGAATCGCGTTTTTCTACCTTAGTTAGTGATTTTGGAATTGAAACTAACTTAACGCGGAGTGCC
TTTGGCTTTCAACTAAAAATTGAACTGGCAATTTTAGTCTATAATTTAGGATTTTTTAATTTTGTGACGAACTAGCACCACGCGT
TGAACCATCCTAAGTTTAAACAAATTCGTCACGAATTACAAGGTAACTATCATTATTTTTATCAGCTGTGCCAGTCGCTTTACCAGCGCTATTGCCCACG
TTGTGTGACGCAACGGCGTAATGTGGCGCACGTTAAAATCGATGACTGTCGATTGTTGGCGCTACTGTGTTTACAAACAACGCTTGGTATCCAATCACAA
CGCCGTTTTTGGCGCTTGATGACCGCCTTTATGCCGCAAAAAATCACCATTAGTCGGTCACGGTTCAACCGTCGCGCACTACAATTATTACCCGTGGTCA
ATGCGATCCGTACTGGTTTGACGCGTGAAGCAGCCCAACCTGGACAGATCGCAATCATTGATAGTTTGCCGAATCCATTGTGTGAACCAGTACGTAAATT
TAGAGCACGTATTTTTGCTGGGCAAGCTAATATTGGCTACAATGCCACTAAGCAAATGTCGTTCTATGGGTTTAAAACCCACATGCTAGTAACCACTGAT
GGCTACATTCTGAATTATGTGGTGACTGCCGCTTCGGTCCATGATACAAAAGTTGCCGTTAGCTTGATTGATGATTGTCCTTGTCCAATTGTTTTGGCCG
ATGTCGGTTATGTAGGCAAGCGACTTGGCGCTGAATTTAAGCAATTAGGCTACACGTTATGGACACCATACCGTTCGAATATGAAGGGGGCCAAGGAACA
TAATAAGCGCGCACTCAAGGCGTTACGTCGCACAATTGAATCGCGTTTTTCTACCTTAGTTAGTGATTTTGGAATTGAAACTAACTTAACGCGGAGTGCC
TTTGGCTTTCAACTAAAAATTGAACTGGCAATTTTAGTCTATAATTTAGGATTTTTTAATTTTGTGACGAACTAGCACCACGCGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 97 | 975 | + | No |
Chemistry : DDE
ORF sequence :
MLNHPKFKQIRHELQGNYHYFYQLCQSLYQRYCPRCVTQRRNVAHVKIDDCRLLALLCLQTTLGIQSQRRFWRLMTAFMPQKITISRSRFNRRALQLLPV
VNAIRTGLTREAAQPGQIAIIDSLPNPLCEPVRKFRARIFAGQANIGYNATKQMSFYGFKTHMLVTTDGYILNYVVTAASVHDTKVAVSLIDDCPCPIVL
ADVGYVGKRLGAEFKQLGYTLWTPYRSNMKGAKEHNKRALKALRRTIESRFSTLVSDFGIETNLTRSAFGFQLKIELAILVYNLGFFNFVTN
VNAIRTGLTREAAQPGQIAIIDSLPNPLCEPVRKFRARIFAGQANIGYNATKQMSFYGFKTHMLVTTDGYILNYVVTAASVHDTKVAVSLIDDCPCPIVL
ADVGYVGKRLGAEFKQLGYTLWTPYRSNMKGAKEHNKRALKALRRTIESRFSTLVSDFGIETNLTRSAFGFQLKIELAILVYNLGFFNFVTN
Blast result :
Comments
ISLpl4 is 63% aa similar to ISScr1. There are 8 copies of ISLpl4 IS in Lactobacillus plantarum CECT 4645 (NCFB 965).
An ISLpl4 copy of O. oeni contained a +1 nucleotide insertion on its transposase encoding gene and, by using anexperimental system, we were able to demonstrate that this specific sequence originates a +1 programmed translational frameshifting. Although the frameshifting process reported here operates at a low rate, this description might represent the first case of a functional +1 frameshifting among IS.
An ISLpl4 copy of O. oeni contained a +1 nucleotide insertion on its transposase encoding gene and, by using anexperimental system, we were able to demonstrate that this specific sequence originates a +1 programmed translational frameshifting. Although the frameshifting process reported here operates at a low rate, this description might represent the first case of a functional +1 frameshifting among IS.
References