ISClte2

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_004565 ND Clostridium tetani
Clostridium tetani E88 plasmid pE88
DNA section
IS Length : 1690 bp

Ends


Left end : CCAGGTTTGTCGTAAATCCCCTGTCTTCAAACATGGGGATATAAGGCAATATATACTTGTTGTATTATATGACCTTAAGTAGTATAATCCAAACATGGAA II struct. : Yes
Right end : TACTGGCATACCCTTAGCTTGAGGTCATACTGGACTATCAGAAATGAACTGTCCTTTAATCACTCAAGAATCCCCTAGATTTATCTATGGGGAGTGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTTTTTCTCCCATTTATATAAAAACATCAA

DNA sequence

CCAGGTTTGTCGTAAATCCCCTGTCTTCAAACATGGGGATATAAGGCAATATATACTTGTTGTATTATATGACCTTAAGTAGTATAATCCAAACATGGAA
CAAAAATATAAATCAAATAATAATATAGTCTACTCATGCAAGTATCACATAGTTTGGTGTCCTAAATATCGTAGAAAAGTTCTTATTGGCAATGTAGAAA
CAAGATTAAAGGAGTTGTTAATTGAAACTTGTGCCGATATTGAAGTCGATATAATCAAAATGGAGTTAATGCCAGACCATGTTCATATGCTTATTGAAGT
AGACCCACAATTTGGAGTTCATAAAGCTATTAAACGAATGAAAGGTAGAACATCCAAAATGTTAAGACAAGAATTTCCACATCTTAAAACCAAACTACCT
ACTCTATGGACTAATAGCTATTTTGTGTCAACTGTTGGTGGTGCTCCACTTTCAGCAATTAAACAATATATTGAAAATCAAAAAACATCACAAAGGAAAT
GATACTGTGCAATTAACCATTAAAATCAAATTAATACCAATGAAAGAGCAAAAACTACATATTCAAGATACTTGTTTAGAATATATTAAAACTGTTAATG
ATATAGTATCACTAATGGTTGCTGAAAATAAAAGTCTTAAACTAACTTCAAAAGATATAATTGCTAATCTTCCAAGTGCTGTTAAAAATCAAGCAATTAG
AGATAGTAAAAGTGTATATTCTAAATATAAGAAAACTAAAATACAAAATTTTCTTAAAAAACCTATGTGTATATGGAACAACCAAAACTATAGTATTGGT
GAAAATTATATTGCTTTTCCAGTAATGATTAATGGTAAATCTAAAAAGATTAAAGTTAAAGCTATCATAGAACAATATCAAAAAGATTTATTAACCAATA
AATTAGGTACTTTAAGAATAACTAAAAAAGCCAATAAATTAATGGCTCAAATTTCTATTGATGTAGTAGAAAAAGAAAATGTAAACAATAAAGCTATGGG
TATAGATTTGGGACTAAAAATACCTGCTGTTGCAGTAACAGAAGATAAAAAAGTAAAATTCTTTGGCAATGGTAGGCAAAACAAATACATTAAAAGAAAA
TATAGATCATTAAGAAAAAAGTTAGGTAAAGCTAAAAAAATTAACAAAATAAAATCTATAAATGATAAAGAACAAAGATATATGAAAGACCAAGACCACA
AAATAAGTAGAGGAATAGTTAATTTTGCTATTCAAAATAATATTTCCGTAATTAGATTAGAAAAACTAACTAATATCAGAAATACGGCAAAAACAAACCG
TAAAAATGAAAAGAATTTGCATACTTGGTCATTCTATCGTCTAGCACAATACATTGAATATAAGGCACTTTTAGAAGGTATTAAAATTGAATATGTTAAT
CCATGTAATACATCTAAACAATGCCCTATTTGTGGCAAATTAAATACTGCTAAAGATAGAAAATATCAATGTTCATGTGGTTATAAAGCACATAGAGATT
TGAATGCTTGTATTAATATAATTAATGCAACTGTTGTAGATGGTAATAGTCTACAAGCCTAAGATACTATAGGTACTGTCTTAGGAAGGGTACTGGCATA
CCCTTAGCTTGAGGTCATACTGGACTATCAGAAATGAACTGTCCTTTAATCACTCAAGAATCCCCTAGATTTATCTATGGGGAGTGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
408 bp135 aa95502+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEQKYKSNNNIVYSCKYHIVWCPKYRRKVLIGNVETRLKELLIETCADIEVDIIKMELMPDHVHMLIEVDPQFGVHKAIKRMKGRTSKMLRQEFPHLKTK
LPTLWTNSYFVSTVGGAPLSAIKQYIENQKTSQRK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1140 bp379 aa4231562+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MCQLLVVLHFQQLNNILKIKKHHKGNDTVQLTIKIKLIPMKEQKLHIQDTCLEYIKTVNDIVSLMVAENKSLKLTSKDIIANLPSAVKNQAIRDSKSVYS
KYKKTKIQNFLKKPMCIWNNQNYSIGENYIAFPVMINGKSKKIKVKAIIEQYQKDLLTNKLGTLRITKKANKLMAQISIDVVEKENVNNKAMGIDLGLKI
PAVAVTEDKKVKFFGNGRQNKYIKRKYRSLRKKLGKAKKINKIKSINDKEQRYMKDQDHKISRGIVNFAIQNNISVIRLEKLTNIRNTAKTNRKNEKNLH
TWSFYRLAQYIEYKALLEGIKIEYVNPCNTSKQCPICGKLNTAKDRKYQCSCGYKAHRDLNACINIINATVVDGNSLQA

 

Blast result :
Comments
ISClte2 is 83% aa similar to IS608 (ORF A) and 40% aa similar to IS606 (ORF B). There are two copies in pE88.
References
1] Brueggemann,H., Baumer,S., Fricke,W.F., Wiezer,A., Liesegang,H., Decker,I., Herzberg,C., Martinez-Arias,R., Merkl,R., Henne,A. and Gottschalk,G. (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (3), 1316-1321