ISNsp2

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003272 ND Nostoc sp.
Nostoc sp. PCC 7120
DNA section
IS Length : 1718 bp

Ends


Left end : GAGTCGTGATGCGTAAAGCCCCCAATTATGAGGGACAAAACCAATAAGTTTTGTCCCTCATACGTCTTTTAAGTGGGGGATATAAGCTTTGGAGGATGAA II struct. : Yes
Right end : GGGAGTTCGACTCTAGGCGTAGGAGATGTTAGACAGTCCCAGACTGCAATCTCTGTCTGATGCCTGAATCCCCCGTTTTCAAAACGGGGGAGTGGCTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
AAAATCCTGTTTCTAAGTCTTACGGCTTACCTCTGTCCACGAtcaa

DNA sequence

GAGTCGTGATGCGTAAAGCCCCCAATTATGAGGGACAAAACCAATAAGTTTTGTCCCTCATACGTCTTTTAAGTGGGGGATATAAGCTTTGGAGGATGAA
TTTATTCATCCGTGATGCGGGTCATTTATGTACTCTAAGACTTTCTGGGTGCTAACTTTCCCTGTAGTGTCAAACATATACGAATGTGTCCACAAACTAG
GTAATCTCATTAACTCTGGAAACTCTTGTCGCAAATATCTTGATGACCTACCCTTAAATGCTTTAACTATTTGAGATATTGAATGATGCGGGTCATATTC
AACCAATAAATGAATATGGTCAGGGGCAATTTCTAGTGCTTTGATAATCCATCTTTTATCATTAGCAACTGACTGAAATATCTCAATACATCGTAATTTT
AATTTTTCATTATTAGCAAAAACCCTCCTACGTCTACATGGTATCCAAACTAAATGAACAGTAGCCAATCCGATTGCGTGGTTGTAGTGATTATATACAG
ATTTCATGTAGATGAGTATTGATTATCTACACTACTAATGATATCATAACCACATGAAAACCTTGAAGTTCAAACTGTACCAGCACAAAAGAAATAGATT
TCTCAAACGCATGATTAATGCAGGTGGGGCAATCTACAACCATTGTATTGCTCTACATAAACGCTACTATCGTATGTGGGGCAAACACTTAAACTGTGCA
AAACTTCAAGCTCATATTGCCAAACTGAGAAAGCGTAACCCATTTTGGCAAACAGTAGGTTCTCAGGCGGTACAAGATATTTGTCAACGCATTGAGAAAG
CCTACCAATTATTTTTTAAACACAACAAGAAGGGGGTTAGACCACCAGGATTTAAGAAGGTAAAAAAATACAAATCATTTACTCTAAAACAAGCAGGTTA
TAAGTTTTTGGATGGAAATAGAGTAAAAATTGGGAGTAAAATTTATCAGTTTTGGAAGTCTAGAGAAATTGAGGGGACAGTCAAAACATTAACTATTAAA
CGCACACCATTAGGTGAGTTGTTTATGGTTGTGGTGGTTGACGAAGGTAACAAGCCAGAAACCAAATTCATGACTGGTAAGATAGCGGGATTTGATTTCG
GTTTAAAAACCTTTCTCACTTGCTCAGACGGTACAACAATTGAGTCTCCCCAGTTTTTTAAGCAATCCCTAAATGCAATTAAAAAAGCCAGCCGACAGCA
TTCTAAGAAATTAAAAGGTTCAGCCAATCGTGAGCAAGCTAGAAAGAACTTAGTACGCAGGTATGAAGATATCTTTAACCGTCGTCGTGATTGGTTCTGG
AAGTTAGCCCATGAGTTAACGGATAAATTTGATGTTCTCTGTTTTGAAACCTTAAACCTTAAAGGAATGCAACGACTTTGGGGAAGGAAAATATCAGACT
TGGCTTTTGGTGAATTACTGCAAATTCTAGAATGGGTTGCCAAAAACAAGAATAAACTGGTTGTTTTCATTGACCCGTGGTATCCCAGTAGCAAAACCTG
CTCTAGCTGTGGACATATTTTAGAAAACCTAGATTTGTCAATTAGACGCTGGCGTTGTCCTTCATGTCAATCTGAAAATGATCGGGATGGAAACGCTAGT
TTGAATATTAAAAGAGTCGGGAGTTCGACTCTAGGCGTAGGAGATGTTAGACAGTCCCAGACTGCAATCTCTGTCTGATGCCTGAATCCCCCGTTTTCAA
AACGGGGGAGTGGCTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
402 bp133 aa507106-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKSVYNHYNHAIGLATVHLVWIPCRRRRVFANNEKLKLRCIEIFQSVANDKRWIIKALEIAPDHIHLLVEYDPHHSISQIVKAFKGRSSRYLRQEFPELM
RLPSLWTHSYMFDTTGKVSTQKVLEYINDPHHG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1125 bp374 aa5541678+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKTLKFKLYQHKRNRFLKRMINAGGAIYNHCIALHKRYYRMWGKHLNCAKLQAHIAKLRKRNPFWQTVGSQAVQDICQRIEKAYQLFFKHNKKGVRPPGF
KKVKKYKSFTLKQAGYKFLDGNRVKIGSKIYQFWKSREIEGTVKTLTIKRTPLGELFMVVVVDEGNKPETKFMTGKIAGFDFGLKTFLTCSDGTTIESPQ
FFKQSLNAIKKASRQHSKKLKGSANREQARKNLVRRYEDIFNRRRDWFWKLAHELTDKFDVLCFETLNLKGMQRLWGRKISDLAFGELLQILEWVAKNKN
KLVVFIDPWYPSSKTCSSCGHILENLDLSIRRWRCPSCQSENDRDGNASLNIKRVGSSTLGVGDVRQSQTAISV

 

Blast result :
Comments
ORF A coordinates are complement (106-507). ISNsp2 is 55% aa (ORF A) and 61% aa (ORF B) similar to IS608.
References
1] Kaneko,T., Nakamura,Y., Wolk,C.P., Kuritz,T., Sasamoto,S., Watanabe,A., Iriguchi,M., Ishikawa,A., Kawashima,K., Kimura,T., Kishida,Y., Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A., Nakazaki,N., Shimpo,S., Sugimoto,M., Takazawa,M., Yamada,M., Yasuda,M. and Tabata,S. (2001) DNA Res. 8 (5), 205-213