ISBf6
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AM042593 | ND | Bacteroides fragilis | Bacteroides fragilis |
DNA section
IS Length : 950 bp
Ends
IR Length : 18/19
IRL : GAAGTGATTTAAACTTATTTCATTCTCTTAAAATCGTATCAAAATGTTAA
IRR : GAAGTGATTTAAACTTTTTGTTTTCGTGAAATTTTCTTCAATAACAAGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTATTAAACAAT | TTTATTCTGA | 0 | |
| CTTCATTGTT | TAATACTAAA | 0 |
DNA sequence
GAAGTGATTTAAACTTATTTCATTCTCTTAAAATCGTATCAAAATGTTAAAGCACAGCGTACAAATTGAATTGTTCCCTATATTATGGTGACCAAACTCA
TATATAGTGAAAGTCCAATGTACACTATGCTCGACAAAGATACGATAAAAAATGAAATATTGCCTCATCTTTCCAAGGCAAAACGTGGTTATGCCACTCA
AAGTTGTCTCATAGAGGTGGTAAACGCCATCTTGTACAAGTTGAAGACCGGTTGTCAATGGGCGTTCCTTCCTGTGGAAGCCCTGTTTAGCGGCAAGGTA
CTGAGCTATGGTGCCGTGTATCATCATTACAACAAATGGAGCAAGTCGGGCGAGTGGAAATCCATGTGGGTCAGGTTGCTTGACAAACACCGCCATAAGT
TGGACATGTCCAGCGTGGATTTGGACGGGAGCCACACGGCTGCACTCCGTGGCGGTGAAGAAGTCGGATACCAGGGACGGAAGAAACGGAAGACGACCAA
CGCACTCTACCTGACAGACAGGCAAGGACTTCCGATTGCCATGTCTTCTCCGAAATCTGGCGAACACCATGACGTACATGACATAGAAAAGGTCATCGGT
GACATGTTCAACGACCTTGAGAAATCCCATATCCGGGTTGATGGTCTTTTCCTCAATGCCGATGCCGGATTCGATTGTGATGTCTTACGTGGTTTCCTGG
GTGAAAAAGAGATCATCGCAAACATCTGCATCAATAAACGGAGAGAAAATGCCGATGACATCTTTGTTGATGATGAACTATATGCTGAAAGGTATTCCAT
CGAACGAACCAACGCTTGGATGGACAGTTTCAGGACGCTACTGAACCGGTTCGATACAACTGTTTCAAGTTGGATGTCTTGGAATTACTTGGCATTTGCA
GTCTTGTTATTGAAGAAAATTTCACGAAAACAAAAAGTTTAAATCACTTC
TATATAGTGAAAGTCCAATGTACACTATGCTCGACAAAGATACGATAAAAAATGAAATATTGCCTCATCTTTCCAAGGCAAAACGTGGTTATGCCACTCA
AAGTTGTCTCATAGAGGTGGTAAACGCCATCTTGTACAAGTTGAAGACCGGTTGTCAATGGGCGTTCCTTCCTGTGGAAGCCCTGTTTAGCGGCAAGGTA
CTGAGCTATGGTGCCGTGTATCATCATTACAACAAATGGAGCAAGTCGGGCGAGTGGAAATCCATGTGGGTCAGGTTGCTTGACAAACACCGCCATAAGT
TGGACATGTCCAGCGTGGATTTGGACGGGAGCCACACGGCTGCACTCCGTGGCGGTGAAGAAGTCGGATACCAGGGACGGAAGAAACGGAAGACGACCAA
CGCACTCTACCTGACAGACAGGCAAGGACTTCCGATTGCCATGTCTTCTCCGAAATCTGGCGAACACCATGACGTACATGACATAGAAAAGGTCATCGGT
GACATGTTCAACGACCTTGAGAAATCCCATATCCGGGTTGATGGTCTTTTCCTCAATGCCGATGCCGGATTCGATTGTGATGTCTTACGTGGTTTCCTGG
GTGAAAAAGAGATCATCGCAAACATCTGCATCAATAAACGGAGAGAAAATGCCGATGACATCTTTGTTGATGATGAACTATATGCTGAAAGGTATTCCAT
CGAACGAACCAACGCTTGGATGGACAGTTTCAGGACGCTACTGAACCGGTTCGATACAACTGTTTCAAGTTGGATGTCTTGGAATTACTTGGCATTTGCA
GTCTTGTTATTGAAGAAAATTTCACGAAAACAAAAAGTTTAAATCACTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 858 bp | 285 aa | 85 | 942 | + | No |
Chemistry : DDE
ORF sequence :
MVTKLIYSESPMYTMLDKDTIKNEILPHLSKAKRGYATQSCLIEVVNAILYKLKTGCQWAFLPVEALFSGKVLSYGAVYHHYNKWSKSGEWKSMWVRLLD
KHRHKLDMSSVDLDGSHTAALRGGEEVGYQGRKKRKTTNALYLTDRQGLPIAMSSPKSGEHHDVHDIEKVIGDMFNDLEKSHIRVDGLFLNADAGFDCDV
LRGFLGEKEIIANICINKRRENADDIFVDDELYAERYSIERTNAWMDSFRTLLNRFDTTVSSWMSWNYLAFAVLLLKKISRKQKV
KHRHKLDMSSVDLDGSHTAALRGGEEVGYQGRKKRKTTNALYLTDRQGLPIAMSSPKSGEHHDVHDIEKVIGDMFNDLEKSHIRVDGLFLNADAGFDCDV
LRGFLGEKEIIANICINKRRENADDIFVDDELYAERYSIERTNAWMDSFRTLLNRFDTTVSSWMSWNYLAFAVLLLKKISRKQKV
Blast result :
Comments
ISBf6 is 49% aa similar to ISMac11.
ISBf6 was found in Bacteroides fragilis strain 388 upstream of a metronidazole resistance gene, nimE, harboured on a small plasmid, pBF388c(ca. 8.3 kb).
ISBf6 was found in Bacteroides fragilis strain 388 upstream of a metronidazole resistance gene, nimE, harboured on a small plasmid, pBF388c(ca. 8.3 kb).
References