ISMma5
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003901 | ND | Methanosarcina mazei | Methanosarcina mazei Go1 |
DNA section
IS Length : 1522 bp
Ends
IR Length : 10/15
IRL : AAAAACACACATGATATTCTTGGAATAACATGCCCAAAAAATGAAAGTCT
IRR : -----TTGGCATGAAAGTCCATGATGAGAAAACTCATCACGTACTCTCTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCCTTTCATAAGCAAA | ATA | AAGGTTAAAAATATA | 3 |
DNA sequence
AAAAACACACATGATATTCTTGGAATAACATGCCCAAAAAATGAAAGTCTTTTTCACTTAGCTTTTTTGATAATAGAACTCCATCATTACCTTTGGACAG
GAAGGTTGTTTTTACTTTGGTTCTCCGAAGCCGTCAAAAAACTAACCATTGAATTGCCATCGAAGGCCCAGATGTGACCTTGATCACGGATGCCAAATTG
CCTTTGAAGCAATTCTACTGTCCTGGAAATGATGGAGTAATGTAAATTGGACGGAGAAATAAACAAATCTTGTGGTTTAGACATTCACAAACGTTTTGTT
ATTGCTACTATCCTCAGTAGATCCGGTGAAAAACAACAACATCGTTTTGCCAGAGATGATGATGGGATTTTAGATCTTAAAAATTTAGTAACCTCTGAAA
AATGTGATGTTGTTGCCTGTGAATCAACAAGTGATTTCTGGGTTCCGATTTATGAGGCATTGATAGATCATTTGCCTGTTATAGTTGGAAATGCTCGAGA
TATGAAAGCATTTACACATAAAAAAACAGATAAAATAGATTCCGAAGTAATTGCAAAACTTGCATTGAATAAGATGGTTCAACCATCAAGAGTTTTTCCG
AAGAAACATAGAGAATTCAGGTCATACGTCAGGCTTCGCCTAACTCTTGTAAGAAAAAGAACGGACATTAAAAATGAAGCTCATGCAATTCTTTCCTCCG
AAATGTTACATCTGGGTGATGTTCTGACTGACATTTTTGGAAAAAATGGTAGAGCAATTCTAGCAGGAATCTCTTCAGGTAAAAATATTGATCAAATTAT
AGAGTCGCTTTCTCCAAATGTTCGGAAAAAGAGTGTTCAGATAAGGGAAATTCTGGATAGAGAAATATCACAGAGTGCTGCAATAAGGCTTCAGATATGT
CTGAACCTTATAAAACATTTAGATGAAGAAATTGAAGTTCTTGAAAGAGAAATTTTCAATTATGCTTATCAAAAGCATAAAAGGGAAATGGAAATTTTGA
TGTCAGTTCCAGGTATTGGGGAGCTTGGTGCGGCAACTCTAATTGCTGAAATAGGAGATTTCAGGGATTTTCCAACGGGAGATAAACTTGCTTCATGGCT
TGGAATAGTTCCTAATGTGTATCAATCTGCAGATAAATACCATAACGGAAGAATCACTAAGAGAGGATCAAAAGTAGCAAGGTGGATTCTAACTCAGATT
GCTCAAGCAGCAGCAAGAAAGAAAAATAGCAGATTAAAGGAGTTTTTTAACAGGAAAAAGAAGACAATTGGATATGCTAAGGCAATTATTGCCTTAGCAA
GGAAAATTGCAACAATAATATGGCATTTGATCACAAACGATGAGATGTACGAAGATGAAACAGGATACCAAAAGGGAGAAGTTAAAAAGAGAAAGATTGT
TGAGGCAGACATATTCTCGGTTGATGAAAGGATAACAATAATTAGTGGAATTATCGCAATTATGGGAAAAAAGGAAGGAGAGAGTACGTGATGAGTTTTC
TCATCATGGACTTTCATGCCAA
GAAGGTTGTTTTTACTTTGGTTCTCCGAAGCCGTCAAAAAACTAACCATTGAATTGCCATCGAAGGCCCAGATGTGACCTTGATCACGGATGCCAAATTG
CCTTTGAAGCAATTCTACTGTCCTGGAAATGATGGAGTAATGTAAATTGGACGGAGAAATAAACAAATCTTGTGGTTTAGACATTCACAAACGTTTTGTT
ATTGCTACTATCCTCAGTAGATCCGGTGAAAAACAACAACATCGTTTTGCCAGAGATGATGATGGGATTTTAGATCTTAAAAATTTAGTAACCTCTGAAA
AATGTGATGTTGTTGCCTGTGAATCAACAAGTGATTTCTGGGTTCCGATTTATGAGGCATTGATAGATCATTTGCCTGTTATAGTTGGAAATGCTCGAGA
TATGAAAGCATTTACACATAAAAAAACAGATAAAATAGATTCCGAAGTAATTGCAAAACTTGCATTGAATAAGATGGTTCAACCATCAAGAGTTTTTCCG
AAGAAACATAGAGAATTCAGGTCATACGTCAGGCTTCGCCTAACTCTTGTAAGAAAAAGAACGGACATTAAAAATGAAGCTCATGCAATTCTTTCCTCCG
AAATGTTACATCTGGGTGATGTTCTGACTGACATTTTTGGAAAAAATGGTAGAGCAATTCTAGCAGGAATCTCTTCAGGTAAAAATATTGATCAAATTAT
AGAGTCGCTTTCTCCAAATGTTCGGAAAAAGAGTGTTCAGATAAGGGAAATTCTGGATAGAGAAATATCACAGAGTGCTGCAATAAGGCTTCAGATATGT
CTGAACCTTATAAAACATTTAGATGAAGAAATTGAAGTTCTTGAAAGAGAAATTTTCAATTATGCTTATCAAAAGCATAAAAGGGAAATGGAAATTTTGA
TGTCAGTTCCAGGTATTGGGGAGCTTGGTGCGGCAACTCTAATTGCTGAAATAGGAGATTTCAGGGATTTTCCAACGGGAGATAAACTTGCTTCATGGCT
TGGAATAGTTCCTAATGTGTATCAATCTGCAGATAAATACCATAACGGAAGAATCACTAAGAGAGGATCAAAAGTAGCAAGGTGGATTCTAACTCAGATT
GCTCAAGCAGCAGCAAGAAAGAAAAATAGCAGATTAAAGGAGTTTTTTAACAGGAAAAAGAAGACAATTGGATATGCTAAGGCAATTATTGCCTTAGCAA
GGAAAATTGCAACAATAATATGGCATTTGATCACAAACGATGAGATGTACGAAGATGAAACAGGATACCAAAAGGGAGAAGTTAAAAAGAGAAAGATTGT
TGAGGCAGACATATTCTCGGTTGATGAAAGGATAACAATAATTAGTGGAATTATCGCAATTATGGGAAAAAAGGAAGGAGAGAGTACGTGATGAGTTTTC
TCATCATGGACTTTCATGCCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1245 bp | 414 aa | 247 | 1491 | + | No |
Chemistry : DEDD
ORF sequence :
MDGEINKSCGLDIHKRFVIATILSRSGEKQQHRFARDDDGILDLKNLVTSEKCDVVACESTSDFWVPIYEALIDHLPVIVGNARDMKAFTHKKTDKIDSE
VIAKLALNKMVQPSRVFPKKHREFRSYVRLRLTLVRKRTDIKNEAHAILSSEMLHLGDVLTDIFGKNGRAILAGISSGKNIDQIIESLSPNVRKKSVQIR
EILDREISQSAAIRLQICLNLIKHLDEEIEVLEREIFNYAYQKHKREMEILMSVPGIGELGAATLIAEIGDFRDFPTGDKLASWLGIVPNVYQSADKYHN
GRITKRGSKVARWILTQIAQAAARKKNSRLKEFFNRKKKTIGYAKAIIALARKIATIIWHLITNDEMYEDETGYQKGEVKKRKIVEADIFSVDERITIIS
GIIAIMGKKEGEST
VIAKLALNKMVQPSRVFPKKHREFRSYVRLRLTLVRKRTDIKNEAHAILSSEMLHLGDVLTDIFGKNGRAILAGISSGKNIDQIIESLSPNVRKKSVQIR
EILDREISQSAAIRLQICLNLIKHLDEEIEVLEREIFNYAYQKHKREMEILMSVPGIGELGAATLIAEIGDFRDFPTGDKLASWLGIVPNVYQSADKYHN
GRITKRGSKVARWILTQIAQAAARKKNSRLKEFFNRKKKTIGYAKAIIALARKIATIIWHLITNDEMYEDETGYQKGEVKKRKIVEADIFSVDERITIIS
GIIAIMGKKEGEST
Blast result :
Comments
ISMma5 is 45% aa similar to ISMmg1. There are 8 full copies and 24 partial copies in Methanosarcina mazei Go1 genome.
References
1]Deppenmeier,U., Johann,A., Hartsch,T., Merkl,R., Schmitz,R.A., Martinez-Arias,R., Henne,A., Wiezer,A., Baumer,S., Jacobi,C., Bruggemann,H., Lienard,T., Christmann,A., Bomeke,M., Steckel,S., Bhattacharyya,A., Lykidis,A., Overbeek,R., Klenk,H.P., Gunsalus,R.P., Fritz,H.J. and Gottschalk,G. (2002) J. Mol. Microbiol. Biotechnol. 4 (4), 453-461