ISMac9

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003552 ND Methanosarcina acetivorans
Methanosarcina acetivorans C2A
DNA section
IS Length : 2199 bp

Ends


IR Length : 21/26

IRL : TGTCAACGGCGGTTAAGAATTCCCCATTTTCGGCGGATTAAAATTCCCCA
IRR : TGTCAACAGCGGTTTAATTTTCCCCACATTGGTCGGTTTAAATTTCCCCA

Insertion site


Left flankDirect repeatRight flankDR Length
ATCTTTTAACTTATTGAAACTACATCG4

DNA sequence

TGTCAACGGCGGTTAAGAATTCCCCATTTTCGGCGGATTAAAATTCCCCAATTCTCAATCCTTGAGAAAAGTTCGAGGATTGTGAATCATGCTGAAAAAG
GAGGATTTATTCTTGATTCGAGATTTAAGTTCACAAAACTTGAGCATTAGTGAAATCGCCAGACAAACCGGTTTTGACAGGAAAACTGTGAGGAAATATC
TCCAGCTGAAAACCTTACCTGAACCCCAGAAACGTCCCGGAAGAAAGAGCAAGCTTGATCCATATAAACCTTATATACTCAAAAAGCTTGAAGAAGGCTC
CTACACTACTGCTCGGCTCTATCGGGAAATCAAAGAAATGGGTTTTGATGGAGGAATGACCATCGTCAAGGACTTTGTAAGAGAAGTCCGACCTCAGCAG
GGAGTCCCTGCTGTATTCCGCTATGAAACAAAACCAGGTGTACAGGCTCAGGTTGACTGGGCAGAGATGGGAACAGTTGAGGTTGATGGAAAGATAAAGA
AACTCTTTTGCTTCAACATGATTCTTGGATATTCCAGGATGAAATATGTTGAATTTACACTGGGCATAGACACTTCCACTCTTATCCAGTGTCATCTGAA
CGCCTTTGAGTACTTTGGAGGATTTACACAGGAGATTCTCTATGATAACATGAAACAGGTTGTTATCAAAAGAGCCTTAAAATCATCAGATTCTGAATGG
AACTCACAGTTTGAGGATTTCTTCAAATGCTTTGGTTTTATTCCACGGTTATGCAGGCCTTACAGGCCTCAGACAAAAGGTAAAATTGAAAATACGGTAG
GCTATGTCAAGAGGGATTTCTTCCTTGGAAGACGATTTACCTCTCTCGAAGACCTGAACGCCCAAGTTCACAGTTGGTTGGAAAGGGTAAATTCAACTGT
CCACGGAACAACCTATCAAATCCCCCTTGAACGTTTTAAGGAGGAGAAACTGATCCCTCTGGATCAGGTTCCTCCTTACAAAGTTGTCCATAAGGAGACC
AGAAAGGTCTCCAGAGACTGTTATATTTCGTTCCTTGGAAATAAGTATTCTGTTCCTTACAGGTTTGCAGGAAGAACTGCAGAGCTTCAGATTTTTGAAG
GAATATTCGAGGTCTATGTTGATTATGAGAAGGTTTGTGAACATGAAATTCTTTCAGGTAATTGTAGGGTTTCCAGAAAAAAGGAACATTTTCAGGGCCT
CCTGAGTGAGATTCTTAAAGAGAATTCAAAATGCAAGAAAGAATTACAGATTCCGTTGAAGTTCTCAGGTCCTGAAGTTGAAAAGAGGTCTCTTGACATC
TATGAAACATTCAGTGACGGTGATTTTGAATGAACAATTTCAGCTATGAGAGACTTCACAGTAACCTGCAATACCTCAAACTGAATACTATTGAAGAGGT
TCTGGACAACTATCTTGAAATTGCTGCAAGAGATAGCAAGACAACAATGGAAGTACTTGATTATCTGTTTGAACAGGAAAAGAAGCACAGAGAAGCTGCT
GCAATTGAGAGAAGGATGAAAAGTGCAGCATTTCCTGTGAAAAAGACGCTTGATGAATTCGATTTTGAGTTTCAGTCATCTATTGATAAAAAAGTCATAG
AAGACCTTGCAACGTTGAGATTTGTTCATAACGTAGAAAACGTTGTTTTCCTTGGTCCTCCCGGAGTTGGAAAGTCTCATCTTGCAATCGCTCTTGGGAT
TGAAGTAGCAAAAGCAGGGATTTCGGTTTACTTTACCAATACAGGAAACCTTATCGAGAAGTTGAAAATAGCAAATCGAGAAGGAATGCTTGAAAAGAAA
CTCAAAGGCTTTATGAAATTTAAAGTTCTGATCATTGATGAAATGGGTTATCTCCCATTTGATGAGGAAGGAGCTCACTGTTTATTTCAGTTGATTTCCA
GACGTTATGAAAAGAGTTCGACCATCTTTACGTCAAATAAATCATATGGAGAATGGGGAGAGATATTCAAAGACCAGGTAATAGCGGCTGCTGTACTTGA
TAGAATTCTCCATCACTGTACTACAATTAACATCAGAGGAGAAAGTTACAGGCTGAAAGAAAGGAAGAAACATGGTATAAAATCAGGAAATATCTACCAG
TAATTTCTAACAAGTTTATGAAAAATTGAGTAAAATTTATATCAAAAGATGGGGAAATTTAAACCGACCAATGTGGGGAAAATTAAACCGCTGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1245 bp414 aa891333+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLKKEDLFLIRDLSSQNLSISEIARQTGFDRKTVRKYLQLKTLPEPQKRPGRKSKLDPYKPYILKKLEEGSYTTARLYREIKEMGFDGGMTIVKDFVREV
RPQQGVPAVFRYETKPGVQAQVDWAEMGTVEVDGKIKKLFCFNMILGYSRMKYVEFTLGIDTSTLIQCHLNAFEYFGGFTQEILYDNMKQVVIKRALKSS
DSEWNSQFEDFFKCFGFIPRLCRPYRPQTKGKIENTVGYVKRDFFLGRRFTSLEDLNAQVHSWLERVNSTVHGTTYQIPLERFKEEKLIPLDQVPPYKVV
HKETRKVSRDCYISFLGNKYSVPYRFAGRTAELQIFEGIFEVYVDYEKVCEHEILSGNCRVSRKKEHFQGLLSEILKENSKCKKELQIPLKFSGPEVEKR
SLDIYETFSDGDFE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
774 bp257 aa13302103+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNNFSYERLHSNLQYLKLNTIEEVLDNYLEIAARDSKTTMEVLDYLFEQEKKHREAAAIERRMKSAAFPVKKTLDEFDFEFQSSIDKKVIEDLATLRFVH
NVENVVFLGPPGVGKSHLAIALGIEVAKAGISVYFTNTGNLIEKLKIANREGMLEKKLKGFMKFKVLIIDEMGYLPFDEEGAHCLFQLISRRYEKSSTIF
TSNKSYGEWGEIFKDQVIAAAVLDRILHHCTTINIRGESYRLKERKKHGIKSGNIYQ

 

Blast result :
Comments
ISMac9 is 93%(ORF1) and 95% (ORF2) aa similar to ISMac3. There are 7 full copies and one partial copy in Mathanosarcina acetivorans C2A genome.
References
1] Galagan,J.E., Nusbaum,C., Roy,A., Endrizzi,M.G., Macdonald,P., FitzHugh,W., Calvo,S., Engels,R., Smirnov,S., Atnoor,D., Brown,A., Allen,N., Naylor,J., Stange-Thomann,N., DeArellano,K., Johnson,R., Linton,L., McEwan,P., McKernan,K., Talamas,J., Tirrell,A., Ye,W., Zimmer,A., Barber,R.D., Cann,I., Graham,D.E., Grahame,D.A., Guss,A.M., Hedderich,R., Ingram-Smith,C., Kuettner,H.C., Krzycki,J.A., Leigh,J.A., Li,W., Liu,J., Mukhopadhyay,B., Reeve,J.N., Smith,K., Springer,T.A., Umayam,L.A., White,O., White,R.H., Conway de Macario,E., Ferry,J.G., Jarrell,K.F., Jing,H., Macario,A.J., Paulsen,I., Pritchett,M., Sowers,K.R., Swanson,R.V., Zinder,S.H., Lander,E., Metcalf,W.W. and Birren,B.(2002) Genome Res. 12 (4), 532-542