ISMac7

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003552 ND Methanosarcina acetivorans
Methanosarcina acetivorans C2A
DNA section
IS Length : 1706 bp

Ends


Left end : ATAAAGAAGCGTAAAACCCCTGAGTCTTTAGCTCAGGGGATATAAGCGTCAACTTCAACCCTGATTCCGTATATAATATTCTGTGGCTTCTTTACTAACG II struct. : Yes
Right end : TAACACCTACGGAACGTGGGGAAGAGCTTGGGGACTTGCCCTCAATAGAGGGAAGAATGAACCAAGAAGCAACTCAGTCTTTAGCTGAGTGGTAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTTATTCAC

DNA sequence

ATAAAGAAGCGTAAAACCCCTGAGTCTTTAGCTCAGGGGATATAAGCGTCAACTTCAACCCTGATTCCGTATATAATATTCTGTGGCTTCTTTACTAACG
TTTCCGATGGTAGAAGCAAAATATCCATCGCTCCACAATGTGTTTTCGCCCCTATAATACTTTTCCAGATATTCTTTTTGTGTTTTCCAAATTCTGTTCG
TAGATTCATGTTTCTGTTTTCGGACAATAGATAGAACACTGACTTTCGTTTCGCTCTTAATTAAGAAATGGATGTGATCTTTGTCAGTTTCCATTTCAAG
AATTTCAAAGTTTGACCCTTTTGAAATATCAATCACAATTTGTTTGAGTTCTTCGCTGATTGGTTCAAGTATAGCTTTTCGATATTTGCAAACAAAGATA
ATATGATACATTAACAGAAATTTGCTATGATTCCTTGTCTCATATTTCATATTTACAAAAAAATATATGAATTGTTAAAGCATATGATCTCTTTGTATGA
TGAAAGCGTTCAAATTTAGGCTCTATCCTACAACTACACAAGCTGATCAACTCAATCAACATATAGGTAGCTGTAGGTTTGTCTATAATTGGGCACTTGA
TCAGAAAATTAAAACATACGAACAAACAGGGAAATCAATTTCCAGATTTGACTTAAACAAATTAATTCCAGCTTTGAAGACCTCTAACGAATGGTTAGGA
GAAGTCAATTCTCAATCATTACAGGGGATGACTAAGCAGGTTGAGTCTGCTTTCACTCGATTTTTCCGAGAGAAGAATGGATTTCCGAAGTTTAAGTCAA
AGAAAAATCCTATCCAGTCTTTTCCCGTACCCCAACACTATTCTGTGAATTTTGAAAACCATACCGTTAAACTTCCTAAAATTGGTGAGATTAAAGCAGT
TCTTCACCGAAAATTTGATGGAGAGCTTAAAACAGCTAGGGTATCAAGGACTTGTAAAGGGCATTACTACATCAGCGTCCTTGTTGAAGACGGTAAAGAA
CTTCCTGAAAAACAGGGATTCTCAGAATCAACAACAGTAGGAATAGATGTGGGTATCAAAGATTTTGCCGTCCTTTCCACAGGAGAAAAGATTGAGAATC
CTAAATACCTGAAAAACTCTTTGCAAAGGTTAAAGGTCCTTCAGAAAAGAGTATCAAGGAAACAAAAAGGCTCTAAAAATAGGGCAAAAGCCAGACAGAG
GCTTGCTGTACTCCACGACAAAATAACAAATCAGAGGAATGACTTCCAGAACAAACTCTCTTTTAGACTTGTTAGCGAAAACCAAGCTGTAGCTCTGGAA
ACTCTGAATGTTAAAGGTATGGTTAAAAATCATCATTTGGCACAGGCTATAAGTGATTCTGCATGGAGCAGCTTTGTAACAAAGTTAGAATATAAAGCAG
AATGGTTTGGAAAAGGCGTATTAAGGATAGGACAATTTGAACCTTCTTCTAAGCTCTGTAACGTGTGTGGATACCATAATAAAGAGCTTCAGTTAAAAGA
CAGAGAATGGACTTGTCCTGATTGTAAAACAAAACATGATAGAGACATAAATGCCGCTATCAATATCAAGAAATTCGCTCTCATAGATCAAAATCTAATT
GGATTGTAACACCTACGGAACGTGGGGAAGAGCTTGGGGACTTGCCCTCAATAGAGGGAAGAATGAACCAAGAAGCAACTCAGTCTTTAGCTGAGTGGTA
GTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
396 bp131 aa55450-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKYETRNHSKFLLMYHIIFVCKYRKAILEPISEELKQIVIDISKGSNFEILEMETDKDHIHFLIKSETKVSVLSIVRKQKHESTNRIWKTQKEYLEKYYR
GENTLWSDGYFASTIGNVSKEATEYYIRNQG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1122 bp370 aa4881609+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MMKAFKFRLYPTTTQADQLNQHIGSCRFVYNWALDQKIKTYEQTGKSISRFDLNKLIPALKTSNEWLGEVNSQSLQGMTKQVESAFTRFFREKNGFPKFK
SKKNPIQSFPVPQHYSVNFENHTVKLPKIGEIKAVLHRKFDGELKTARVSRTCKGHYYISVLVEDGKELPEKQGFSESTTVGIDVGIKDFAVLSTGEKIE
NPKYLKNSLQRLKVLQKRVSRKQKGSKNRAKARQRLAVLHDKITNQRNDFQNKLSFRLVSENQAVALETLNVKGMVKNHHLAQAISDSAWSSFVTKLEYK
AEWFGKGVLRIGQFEPSSKLCNVCGYHNKELQLKDREWTCPDCKTKHDRDINAAINIKKFALIDQNLIGL

 

Blast result :
Comments
The first ORF, tnpA, is located on the complement(60..455). There is a palindromic sequence at position 22 to 44 : CCCCTGAGTCTTTAGCTCAGGGG.
There are 3 full copies and 14 partial copies of ISMac7 in Methanosarcina acetivorans C2A genome.
References
1] Galagan,J.E., Nusbaum,C., Roy,A., Endrizzi,M.G., Macdonald,P., FitzHugh,W., Calvo,S., Engels,R., Smirnov,S., Atnoor,D., Brown,A., Allen,N., Naylor,J., Stange-Thomann,N., DeArellano,K., Johnson,R., Linton,L., McEwan,P., McKernan,K., Talamas,J., Tirrell,A., Ye,W., Zimmer,A., Barber,R.D., Cann,I., Graham,D.E., Grahame,D.A., Guss,A.M., Hedderich,R., Ingram-Smith,C., Kuettner,H.C., Krzycki,J.A., Leigh,J.A., Li,W., Liu,J., Mukhopadhyay,B., Reeve,J.N., Smith,K., Springer,T.A., Umayam,L.A., White,O., White,R.H., Conway de Macario,E., Ferry,J.G., Jarrell,K.F., Jing,H., Macario,A.J., Paulsen,I., Pritchett,M., Sowers,K.R., Swanson,R.V., Zinder,S.H., Lander,E., Metcalf,W.W. and Birren,B.(2002) Genome Res. 12 (4), 532-542