ISfinder Mirror Site

ISMac3

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_003552	ND	Methanosarcina acetivorans	Methanosarcina acetivorans C2A

DNA section

IS Length : 2199 bp

Ends

IR Length : 16

IRL : TGTCAAGGGCGGTTAAGAATCTTCCAATTACGGCGGTTTAAAAACTTCCA
IRR : TGTCAAGGGCGGTTAACTTTTTTCCATTTTGGGCGGTTTAGAATTTGCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCATATTCAA	ACTT	ACTAATATCG	4

DNA sequence

TGTCAAGGGCGGTTAAGAATCTTCCAATTACGGCGGTTTAAAAACTTCCAATTCTCAATCCTTGTGAAAAGTTCGAGGATTGTAGATAATGCTGAAAACG
GAGGAATGGCTATTGATACGAGATTTGTATTCACAAGGCTTCAGCATCAGTGAGATCTCTAGAAGAACAGGTTATGCTAGGGAAACTGTGAGGAAATATC
TTAAAAAGAAAACTGCCCCAGAACCTCAGAAACGTCCGCCAAAACCGAGTAAACTTGATCCTTTCAAACCTTACATACAAGAAAAACTCAAAGAAGGTCC
TTATACTGCTGTTCGCCTTTATAGGGAAATCAAAGAAATGGGTTTTGATGGAGGAAAAACCATAGTCAAGGACTTCGTAAGAGAAGTCCGACCTAAACAG
GGAGTCCCTGCTGTACTCCGCTATGAAACAAAACCAGGTGTACAGGCTCAGGTTGACTGGGCAGAGATGGGAACAGTTGAGGTTGATGGAAAGGTAAAGA
AACTCTTTTGCTTCAACATGATTCTTGGATATTCCAGAATGAGATATGTTGAATTTACACTGAGCATAGACACTCCCACTCTTATTCAGTGTCATCTGAA
CGCTTTTGAGTACTTTGGAGGATTTACACAGGAGATCCTCTATGATAACATGAAACAGGTTGTTATCAAAAGAGCCTTAAAATCATCAGATTCCGAATGG
AACCCACAGTTTGAGGAGTTCTTCAAATGCTTTGGTTTTATTCCACGGTTATGCAGGCCTTACAGGCCTCAGACAAAAGGGAAAATTGAAAATACAGTCG
GTTTCGTCAAGAGGGATTTCTTCCTTGGAAGAAGGTTTACCTCTCTCGAAGACCTGAACGCCCAAGTTCACAGGTGGTTGGAAAGGGTAAATTCAACTGT
CCACGGAACAACCTATCAAATCCCTCTTGAACGCTTTAAGGAGGAGAAACTGAGCCCTCTGGATCAGGTTCCTCCTTACAAAGTTGTCCATAAGGAGACC
AGAAAGGTCTCCAGAGACTGTTATATTTCGTTCCTTGGAAATAAGTATTCTGTTCCTTACAGGTTTGCAGGGAGAACTGCAGAGCTTCAGATCCTTGAAG
GAATATTCGAGGTCTATGTTGATTATGAGAAAGTCTGTGAACATGAAATCCTTCCTGGAAACTGCAGAGTTTCAAGGAAAAAGGAACATTTCCAGGGTCT
CCTGAGTGAGATTCTTAAAGAGAACTCAAAATGCAAAAAAGATTCACAGATCCCGTTGAAGTTCTCAGATCCCGAAGTTGAAAAAAGGTCTCTTGATGTC
TATGAAATATTTAGTGAAGGTGGTTTTGAATGAACAACTTCACCTATGAGAGACTTCACAATAACCTGCAATACCTGAAACTTAATTCTATCGAAGAGCT
TCTGGATAACTACCTTGAAATTGCTGCAAGGGACAACAAGACAACAATGGAAGTCCTTGATTACTTGTTTGAACAGGAAAAGAAACACAGAGAAGCTGTT
GCAATTGAGAGAAGGATGAAAAGTGCAGTTTTTCCCGTTAAAAAGACTCTTGAGGAATTCGATTTTGAATTTCAGAAATCCATTGATAAAAAAGCAATCG
AAGACCTTGCAACCTTGAGATTTGTTCATAATTCAGAGAATGTCGTTTTCCTTGGTCCTCCCGGAGTTGGAAAGTCTCATCTTGCAATCGCTCTTGGGAT
TGAAGTAGCAAAAGCAGGGATTTCGGTTTACTTTACCAATACAGGAAACCTTATCGAGAAGTTGAAAATAGCAAATCGAGAAGGAATGCTTGAAAAGAAA
CTAAGGGACTTGATGAAATATAAAGTGCTGATAATTGACGAAATAGGGTATCTCCCATTTGACGAAGAAGGAGCTCACTGCCTATTTCAGCTGATCTCAA
GACGGTATGAAAAGAGTTCAACGATCTTGACATCAAATAAATCATATGGAGAATGGGGAGAGATATTCAAGGACCATGTAATAGCGGCTGCTGTACTTGA
TAGGATTCTCCACCATTCAACTACGATTAACATCAAAGGGGAAAGTTACAGGCTGAAAGAAAGGAAGAAACAGGGAATAAAAACAGGAAATATATGCCAG
TAATTTCTAAAAGTTTATGAAAAATTGAGTAAAATTTATATTAAAAGGTTGGCAAATTCTAAACCGCCCAAAATGGAAAAAAGTTAACCGCCCTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1245 bp	414 aa	89	1333	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLKTEEWLLIRDLYSQGFSISEISRRTGYARETVRKYLKKKTAPEPQKRPPKPSKLDPFKPYIQEKLKEGPYTAVRLYREIKEMGFDGGKTIVKDFVREV
RPKQGVPAVLRYETKPGVQAQVDWAEMGTVEVDGKVKKLFCFNMILGYSRMRYVEFTLSIDTPTLIQCHLNAFEYFGGFTQEILYDNMKQVVIKRALKSS
DSEWNPQFEEFFKCFGFIPRLCRPYRPQTKGKIENTVGFVKRDFFLGRRFTSLEDLNAQVHRWLERVNSTVHGTTYQIPLERFKEEKLSPLDQVPPYKVV
HKETRKVSRDCYISFLGNKYSVPYRFAGRTAELQILEGIFEVYVDYEKVCEHEILPGNCRVSRKKEHFQGLLSEILKENSKCKKDSQIPLKFSDPEVEKR
SLDVYEIFSEGGFE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
774 bp	257 aa	1330	2103	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNNFTYERLHNNLQYLKLNSIEELLDNYLEIAARDNKTTMEVLDYLFEQEKKHREAVAIERRMKSAVFPVKKTLEEFDFEFQKSIDKKAIEDLATLRFVH
NSENVVFLGPPGVGKSHLAIALGIEVAKAGISVYFTNTGNLIEKLKIANREGMLEKKLRDLMKYKVLIIDEIGYLPFDEEGAHCLFQLISRRYEKSSTIL
TSNKSYGEWGEIFKDHVIAAAVLDRILHHSTTINIKGESYRLKERKKQGIKTGNICQ

Blast result :

Comments

ISMac3 is 58%(ORF1) aa similar to IS5376 and 66% (ORF2) to IS21.
There are 11 full copies and one partial copy in Methanosarcina acetivorans C2A genome.

References

1] Galagan,J.E., Nusbaum,C., Roy,A., Endrizzi,M.G., Macdonald,P., FitzHugh,W., Calvo,S., Engels,R., Smirnov,S., Atnoor,D., Brown,A., Allen,N., Naylor,J., Stange-Thomann,N., DeArellano,K., Johnson,R., Linton,L., McEwan,P., McKernan,K., Talamas,J., Tirrell,A., Ye,W., Zimmer,A., Barber,R.D., Cann,I., Graham,D.E., Grahame,D.A., Guss,A., Hedderich,R., Ingram-Smith,C., Kuettner,C.H., Krzycki,J.A., Leigh,J.A., Li,W., Liu,J., Mukhopadhyay,B., Reeve,J.N., Smith,K., Springer,T.A., Umayam,L.A., White,O., White,R.H., de Macario,E.C., Ferry,J.G., Jarrell,K.F., Jing,H., Macario,A.J.L., Paulsen,I., Pritchett,M., Sowers,K.R., Swanson,R.V., Zinder,S.H., Lander,E., Metcalf,W.W. and Birren,B.(2002) Genome Res. 12 (4), 532-542