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Transposon
Name: TnSau9       (Synonyms: Tn7228)
Family: Compound Transposon        Group: ISSau9
Evidence of Transposition: yes
 Host     

Host Organism:Staphylococcus warneri Molecular Source:plasmid pSCFS6
Place of Origin:Denmark Date of Isolation:2006
Other Geographic Information:porcine

 Map     



 Terminal Inverted Repeats (IR)     

IRL (Length: 27 bp)TGTATATGTTAACTTAAAACTGAGCCA
IRR (Length: 27 bp)TGTATTTCGCAACTGAAAATTGAGCCA

 Sequence     
DNA SequenceLength  9594 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TGTATATGTT AACTTAAAAC TGAGCCACCA ACGCAATTGA AAACTGAGCC ACTTCTGTAG GAAAATAACC CTAGCACAAT CGTTAGGGGG AAAACAGGAG 100
TGATTACGTT GAACAAGAAG CAACAGGTGA TTCAGCTGCA TCTTCAAGAT GTCAGCCAAA GACAAATTTC CACAGAAGTG AAGGTCTCTA GAAACACCGT 200
ATCCAAATAT ATCGAAGCCT TTGAGAAGAG TAAGGCGACC GATGTTCGTA ACCTGCCGGT TACAGAAGAT ATTTTATCGC CTCCCTCCTA CAAGAAAAGG 300
GCTGGAGTGA AACGTAAAAT GACGGAAGAG ATTGTGGAAA GGCTCAAGGG GTTCATTCAG GACAATGAAT GGAAACGCAA AAACAACATG AGCAAGCAAC 400
AAATGAAAAA GATTGATATG TATGAAAAAC TGCAAGAAGA AAGGTACGAT ATCGGCTATA CAACGGTTCG GAATTTCGTC AATAGCGAGG AGAGACGAAC 500
AAAAGAGGTC TATATTCGCC AACGCTATTC AGCGGCCTGG GAAGTGGAAT TTGATTGGGG AGAAGTAAAA CTGGTGATTG ACGGGAAACA GAAGTCCTAT 600
TCACTGGCGG TCTTCACCTT GGCTTACAGC AATTACCGGT TTGCGCTGCT TTATGAATCC GAGACGATGA TCTGCATACA GGATGCACAT ACCAAACTGA 700
TTGACCACTT AGGTTTCGTT CCCTCTGTTT TCACTTACGA CAATATGCGA ACCGTGGTGA AATCGTTTGT CGGAACAGAC CGGAAAATCA CGGATGGCAT 800
GATCAACCTC TCCAATTACT ATGAATTCCG GATCCGGCTC TGCGAGCCAA GAAAAGGAAA CCAGAAGGGG CATGTGGAAC GGAGTGTGGA GGTTGTTCGC 900
AGAAAAGCAT TTTCGTATGA CATCGCATTC CCTTCTCTTG AAGACGCCCA GAAACGTCTA GCCCAAACCA TTCTGTCGCT CAATAAACGG ATTCACCATG 1000
AAAAGAAACT CGCTCATATA GAATTGAAAG ACCAGGAAAA AGTTGCCGCT GCTCAAGGGG CGCTTCCTCT GCCATTCGAT GTTTCGGAAG TCGTGGAATG 1100
TCGGGTAGAT AAATATAGCA CCGTCACGAT TAAGCAAAAT CGGTATTCCG TACCTGAAGG TCATGTAGGC GCATGGATTC GTGCAAAGGT AAGTGCGGAT 1200
TCAGTCCGTC TCTTTATTGA AGGGGAATTC GTCGCAGAAC ATCGGAGAAA TTGGGGTGTT CATCAATGGG AAATGGATCT CTATCATTAC ATAAAAACTT 1300
TCACAAAGAA AAAAGGCGCT TTAGCTCAAA GTGAATGTTT GAGCCAAGCA CCAAATCAAA TTAAAAAACT CTTCGAAAAC CGTTATATCG GAAAAGAGAA 1400
AGATTTTCTG GAGCTACTCC TTTATGTACG TGAGAAAAAT CAGTGGGATA ACGTGATGGC TGCCGTCCGA CAGATTGAGA CAAAAGGTCT CGGCGACATC 1500
ACGACCGAAA AGCTAATCTT TCTGAGCGAA CAAACAGATC TGGTACCTGC GGTACCGCTG CATCCAGACG AGACGGTCGA ACAGGCGCTT CAAAACATTA 1600
AGGCCTTTTC AGCGATGTTC AATCAAGTCG ATGAAGGAGT GCTTGAACAT GGATAAAAAG CAAAGCGTCA TCGACCTGTG CAAAGAATTA CGTCTTCCTG 1700
GCATCCGTGA GTTGGTCGAA GAAGACGTAG CATTCCAGGA AACGACACCT GCGATTGACC TGTTACACAC CGTGTTAACG AAGGAAAAGA ATGACCGCTG 1800
GATCCGGTCT AAATCCACAC GGATCCGCCG TGCGAACTTT CCGGAGAAAA AGCTGCTGGA GGAGATTGAT ACTCGAGCGC TGCCGACAGA TGCGCAGCAA 1900
AAACTGCCCA ACTTACAGAC GCTCGATTTC ATCAAAGCTG GACAGAATGT GATTCTGACC GGTTCTCCAG GAACCGGCAA GAGCCACCTG GCGATCGGCT 2000
TAGGGGTAGA AGCTTGTTTA GCGGGCTACA AGGTTTATTT CGCCAGTGTC TCGTCCTTAA TCAATCAGTT GAAGGAAAGC CGGTCCGAAA GGACCTTACG 2100
CTCTTTCGAA TTGCGATTTG AGAAATATGA CCTCGTTATC ATTGATGAAT TAGGCTACAT TTCCTTCGAT AAAGAAGGGG CTGAATTACT GTTTACACAT 2200
TTGTCGTTGC GTGCTGGTCG ATCCTCAACC ATTATTACGA GCAACCTGTC TTTTGCGAAA TGGGAAGAGG TATTCCATGA TCCTATTTTG ACCGCCGCAT 2300
TGACGGATCG CTTGACGCAT AAATCGCACG TGGTGAATAT GATCGGCCCA TCTTACCGAA TGAGAGAAAC GCAAAAGTGG CTTGAAAATA GCCATTCATA 2400
AGTGGCTCAA AATTAAATTG CGAAATGGCT CAATTTTCAG TTGCGAAATA CACCCCCCAC CTTCTATCTC GTCCAGTTGG TTTCCCCAGT CCATAAATAT 2500
CAGATGAAGA CAAGGCACTA AAAGTTTTTA TAAAATTGGT TGATTTAGGT TTTACATTAT GTTGCTAACT TTAATATTTA CATTTGAATA TTAAAATGTT 2600
ACAGTACAGG CTAAGTATAT TAATTAGTGA AAGGAGGTAA TAGCTATGTT AACTGTTTTT AGAATCTTAT TCGGTGAAAA TACAAAAATC GGAAACCGTT 2700
TAGCTATTAA AGGGAGTAGT CTGTCCTTTT TTAAACTAAA CAATGATATA AAAACAGTTG CCGGAGCTTT GCCTCTACTA TGGATAAGTT TAAAACAAGA 2800
TCATCAACAT ACTTGTTTTA TGTAGTGAAA TATGCATATT TGTATGTTTT GACTTTCGGC ACCGGTAATT TTTATCTGTG TTTTTTTTGT GTGCAAAAAG 2900
AAATTGATTC TTAACTAGAG CAAATTGTGA AAGGATGAAA GAAATGAATT TTAATAATAA AACAAAGTAT GGTAAAATAC AGGAATTTTT AAGAAGTAAT 3000
AATGAGCCTG ATTATAGAAT AAAACAAATA ACCAATGCGA TTTTTAAACA AAGAATTAGT CGATTTGAGG ATATGAAGGT TCTTCCAAAA TTACTTAGGG 3100
AGGATTTAAT AAATAATTTT GGAGAAACAG TTTTGAATAT CAAGCTCTTA GCAGAGCAAA ATTCAGAGCA AGTTACGAAA GTGCTTTTTG AAGTATCAAA 3200
GAATGAGAGA GTAGAAACGG TAAACATGAA GTATAAAGCA GGTTGGGAGT CATTTTGTAT ATCATCACAA TGCGGATGTA ATTTTGGGTG TAAATTTTGT 3300
GCTACAGGCG ACATTGGATT GAAAAAAAAC CTAACTGTAG ATGAGATAAC AGATCAAGTT TTATACTTCC ATTTATTAGG TCATCAAATT GATAGCATTT 3400
CTTTTATGGG AATGGGTGAA GCTCTAGCCA ACCGTCAAGT ATTTGATGCT CTTGATTCGT TTACGGATCC TAATTTATTT GCATTAAGTC CTCGTAGACT 3500
TTCTATATCA ACGATTGGTA TTATACCTAG TATCAAAAAA ATAACCCAGG AATATCCTCA AGTAAATCTT ACATTTTCAT TACACTCACC TTATAGTGAG 3600
GAACGCAGCA AATTGATGCC AATAAATGAT AGATACCCAA TAGATGAGGT AATGAATATA CTCGATGAAC ATATAAGATT AACTTCAAGG AAAGTATATA 3700
TAGCTTATAT CATGTTGCCT GGTGTAAATG ATTCTCTTGA GCATGCAAAC GAAGTTGTTA GCCTTCTTAA AAGTCGCTAT AAATCAGGGA AGTTATATCA 3800
TGTAAATTTG ATACGATACA ATCCTACAAT AAGTGCACCT GAGATGTATG GAGAAGCAAA CGAAGGGCAG GTAGAAGCCT TTTACAAAGT TTTGAAGTCT 3900
GCTGGTATCC ATGTCACAAT TAGAAGTCAA TTTGGGATTG ATATTGACGC TGCTTGTGGT CAATTATATG GTAATTATCA AAATAGCCAA TAGTATTGAA 4000
CAAGGTATAT AGCTGATATA ATAAGGTGAA TTTTTAATAA TATTAATGCA ATTAATATTA TTTTGATAAC TCAATTATGC TAGTATTTTC TTCAAATATG 4100
AATGGACTAC TGGGTGGAAG ATAATTAACT AATCTGTATT CTTCTAATTT GAAATGAATA AGTTTCAAAG TACTAAGATA TAGGATTAGT CATCCATTTT 4200
TTTTAAATCA GTTTAACTAG ATGTTTTGTT TGTCGAATGA CAAAAAACTC TTATTTAGTA TAAATGAATA GATAATCCTT CCAATAATAA TTGGGAGGAT 4300
TTTTTATTCT CCCTAAATAC AAATGTAAGT TAATGAAATA TATACTTGAA GATGTTTATG GCATTCTAAA AATGTAGTCT TTGGCAGTTA ATTTATGTAG 4400
TCTCTCTAGA GAACAACTTG AAAGTATTCG GGAATGCGTT CAGCTTCATA AAACCCTCCT CAATTATATA ATCTGAAACC CTCTTGCTAA GTTCTTCTTC 4500
AACTTTTTGA AAACCTTTTA ATATCATTTG ATTTCTCACT TTATTAGCGT TCGTAAAAGT ATACTTTTAC AGACAAAAGA GGAAGGCGGG CGCTAATCAG 4600
CATCCGTCTT CCTCTTGTGT TCTATTATTC CATTTAACGC TACTACCGAT AAGGTGTCAT ATGTAAACCA ACTATCCAGA AAAGTTTTTC TAGTAAAACC 4700
TTATAGTTCT ATGCTTTAAA GTAAGCATCA AATAGAGAGT ATTGTGTATG ACAGTTCGAC AGTTTGAGCT ATTAATTCAT CGCCGTTAAT CAACTTAATA 4800
ATCATTGTAA TAGATTTGTA TTCCAGCTCT TTAATTGGTT GATTAATAGT TGAGATAGTA AGTGCCTGTG CGATGGGCTG ATTATCGAAG CCCATCGCAG 4900
CTAAATCTTG TGGGATACGA ATTGTATCCC ATGAGGAGGT TGAACTCGAA TACCAAAAAT CACCTGAAAA TAGTAGGGAT ATGCAAAAGG AATACATTCG 5000
TGTTATCCTT AAATATGAAA AGCGAAATAT TTATAATTTC GCTTTTCTAA AAAGTCATTA GGGCATATAA TTATTTTAAA TAAATAGACG AAAGGACTGA 5100
CTGTATAATA ATGATTTACT AATTCAACTT ATTTGAAATA AATATTTCAA ACTCTAAGTT ATAGGATTAG TCTGATCATT TTTATAAGTC GGTTTGTGAC 5200
ACCTATTGCG TCAAATGCTG TTGGTACAAT AGGGACTTTT AAATATGAGA CCAGATTAAT CCCTTCCAAA TTCTTTGGAA GGATTTTTAT TTTCTTCCTA 5300
TAAAAAAGTA TACTTTTCTA TACCTTTAAC TGCATTCTAA TGAAAAGGAG AATTAACATG GTTTTAGAAT TGGATGTTAC ACATGAATTA CTCCGTATAA 5400
GTCGTTATAA TCTATCAAAC ATATACGGAG TAATTTAGAA TAGATGGATA TACCGACTTT GTATTAAAAG AAATCATAAT ATACAAAGGA GGAATAAAAA 5500
TGTCAATGAT ACATGTACAA AATTTAACTT TCTCTTATCC GAGTAGTTTT GATAATATCT TTGAAGATGT AAGCTTTCAA ATTGATACAG ATTGGAAGCT 5600
TGGATTTATT GGTCGAAATG GACGAGGGAA AACAACCCTT TTTAATTTAT TACTAGATAA ATTTGAATAT AGGGGGAAAA TCATTTCTTC GGTCGATTTT 5700
AACTACTTCC CATATCCAGT AGAAGATAAA AGTAAGTATA CACATGAAAT TTTAGAAGAA ATATGCCCTC AAGCTGAGGA CTGGGAATTT CTTCGAGAAA 5800
TAGCTTATTT AAATGTGGAT GCCGAAGCCA TGTACCGTCC TTTTGAAACT TTATCAAACG GTGAACAAAC AAAGGTATTG CTTGTTGCTC TATTTTTAAA 5900
CGAAGGACAA TTTTTATTAA TTGATGAACC AACAAATCAT TTAGATACTG AAGCTCGTAA GACGGTTTCG AATTACTTGA GGAAGAAAAA AGGGTTTATT 6000
TTAATTTCTC ATGACCGTAA CTTTTTAGAT GGCTGTGTTG ATCATATCTT GTCTATAAAT AGAGCAGATA TTGAGGTTCA AAGTGGAAAT TATTCCTCAT 6100
GGAAGTTGAA CTTTGACCGA CAGCAGGGAC ATGAACAAGC AACAAATGAA CGCTTGCAGA AGGATATTGG AAGGTTAGAA CAATCTACAA AACGTTCGGC 6200
TGGTTGGTCT AACCGAGTCG AAGCTTCAAA AAATGGAACA ACGAATTCTG GTTCTAAATT GGACAAAGGT TTTGTAGGAC ATAAAGCAGC AAAAATGATG 6300
AAACGATCTA AGAACCTTGA GGCTCGACAG CAAAAATCGA TTGAAGAAAA GTCAAAGCTT CTAAAAAACA TTGAAAAAAC GGAGTCCCTA CAGTTTGAAC 6400
CAGTGGAATA TAAATCGAAG GAACTCATTC AATTAACAGA TGTGTCTGTC ATATATGATG GGCAAGTTGT CAACAAACCA ATAAGTTTTA ATGTTGAACA 6500
AGGAGATAGA ATTGTACTGG ATGGAAAGAA CGGCAGTGGA AAAAGTAGTA TTTTAAAATT AATCTTAGGC GATCCAATAC AGTATACAGG CACGTTAAAT 6600
ACGGGTTCTA ACCTGATAAC TTCTTATGTT CAGCAAGACA CCTCTCATTT AAAGGGGATG CTAGCTGACT TTATTGAAGA AAATGAGATT GATGAATCGT 6700
TGTTTAAGGC CATCCTGAGA AAGCTAGATT TTGACCGAGT ACAGTTTGAA AAAGATATAT CTCATTATTC AGGTGGTCAG AAGAAAAAAT TGCTTATCGC 6800
TAAAAGTTTA TGTGAAAAAG CTCACCTATA TATTTGGGAT GAACCATTAA ACTTTATTGA TATTTACTCT CGAATGCAAA TTGAAGAGCT TATTCAAACC 6900
TTTAATCCGA CTATGGTTTT TGTTGAACAT GACCAGACCT TCCAAGAGAC AATATCAACA AAAATAATAA AAATATAATG AAAAACGAGG GTATAGAAAT 7000
AAATCTCTGT CCCCTTCTCG ACTCTCTTCA AATAACAGAG GCTTGTTATC GAAACAAGCT AAATATAAAA ATCGATTCCT AAACTTCAAG GAATCGATTT 7100
TTTGCTGCAA TTTTCTTAAC GTAAATGTTC AAATAGCCCC CATGTATATG TTAACTTAAA ACTGAGCCAC CAACGCAATT GAAAACTGAG CCACTTCTGT 7200
AGGAAAATAA CCCTAGCACA ATCGTTAGGG GGAAAACAGG AGTGATTACG TTGAACAAGA AGCAACAGGT GATTCAGCTG CATCTTCAAG ATGTCAGCCA 7300
AAGACAAATT TCCACAGAAG TGAAGGTCTC TAGAAACACC GTATCCAAAT ATATCGAAGC CTTTGAGAAG AGTAAGGCGA CCGATGTTCG TAACCTGCCG 7400
GTTACAGAAG ATATTTTATC GCCTCCCTCC TACAAGAAAA GGGCTGGAGT GAAACGTAAA ATGACGGAAG AGATTGTGGA AAGGCTCAAG GGGTTCATTC 7500
AGGACAATGA ATGGAAACGC AAAAACAACA TGAGCAAGCA ACAAATGAAA AAGATTGATA TGTATGAAAA ACTGCAAGAA GAAGGGTACG ATATCGGCTA 7600
TACAACGGTT CGGAATTTCG TCAATAGCGA GGAGAGACGA ACAAAAGAGG TCTATATTCG CCAACGCTAT TCAGCGGCCT GGGAAGTGGA ATTTGATTGG 7700
GGAGAAGTAA AACTGGTGAT TGACGGGAAA CAGAAGTCCT ATTCACTGGC GGTCTTCACC TTGGCTTACA GCAATTACCG GTTTGCGCTG CTTTATGAAT 7800
CCGAGACGAT GATCTGCATA CAGGATGCAC ATACCAAACT GATTGACCAC TTAGGTTTCG TTCCCTCTGT TTTCACTTAC GACAATATGC GAACCGTGGT 7900
GAAATCGTTT GTCGGAACAG ACCGGAAAAT CACGGATGGC ATGATCAACC TCTCCAATTA CTATGAATTC CGGATCCGGC TCTGCGAGCC AAGAAAAGGA 8000
AACCAGAAGG GGCATGTGGA ACGGAGTGTG GAGGTTGTTC GCAGAAAAGC ATTTTCGTAT GACATCGCAT TCCCTTCTCT TGAAGACGCC CAGAAACGTC 8100
TAGCCCAAAC CATTCTGTCG CTCAATAAAC GGATTCACCA TGAAAAGAAA CTCGCTCATA TAGAATTGAA AGACCAGGAA AAAGTTGCCG CTGCTCAAGA 8200
GGCGCTTCCT CTGCCATTCG ATGTTTCGGA AGTCGTGGAA TGTCGGGTAG ATAAATATAG CACCGTCACG ATTAAGCAAA ATCGGTATTC CGTACCTGAA 8300
GGTCATGTAG GCGCATGGAT TCGTGCAAAG GTAAGTGCGG ATTCAGTCCG TCTCTTTATT GAAGGGGAAT TCGTCGCAGA ACATCGGAGA AATTGGGGTG 8400
TTCATCAATG GGAAATGGAT CTCTATCATT ACATAAAAAC TTTCACAAAG AAAAAAGGCG CTTTAGCTCA AAGTGAATGT TTGAGCCAAG CACCAAATCA 8500
AATTAAAAAA CTCTTCGAAA ACCATTATAT CGGAAAAGAG AAAGATTTTC TAGAGCTACT CCTTTATGTA CGTGAGAAAA ATCAGTGGGA TAACGTGATG 8600
GCTGCCGTCC GACAGATTGA GACAAAAGGT CTCGGCGACA TCACGACCGA AAAGCTAATC TTTCTGAGCG AACAAACAGA TCTGGTACCT GCGGTACCGC 8700
TGCATCCAGA CGAGACGGTC GAACAGGCGC TTCAAAACAT TAAGGCCTTT TCAGCGATGT TCAATCAAGT CGATGAAGGA GTGCTTGAAC ATGGATAAAA 8800
AGCAAAGCGT CATCGACCTG TGCAAAGAAT TACGTCTTCC TGGCATCCGT GAGTTGGTCG AAGAAGACGT AGCATTCCAG GAAACGACAC CTGCGATTGA 8900
CCTGTTACAC ACCGTGTTAA CGAAGGAAAA GAATGACCGC TGGATCCGGT CTAAATCCAC ACGGATCCGC CGTGCGAACT TTCCGGAGAA AAAGCTGCTG 9000
GAGGAGATTG ATACTCGAGC GCTGCCGACA GATGCGCAGC AAAAACTGCC CAACTTACAG ACGCTCGATT TCATCAAAGC TGGACAGAAT GTGATTCTGA 9100
CCGGTTCTCC AGGAACCGGC AAGAGCCACC TGGCGATCGG CTTAGGGGTA GAAGCTTGTT TAGCGGGCTA CAAGGTTTAT TTCGCCAGTG TCTCGTCCTT 9200
AATCAATCAG TTGAAGGAAA GCCGGTCCGA AAGGACCTTA CGCTCTTTCG AATTGCGATT TGAGAAATAT GACCTCGTTA TCATTGATGA ATTAGGCTAC 9300
ATTTCCTTCG ATAAAGAAGG GGCTGAATTA CTGTTTACAC ATTTGTCGTT GCGTGCTGGT CGATCCTCAA CCATTATTAC GAGCAACCTG TCTTTTGCGA 9400
AATGGGAAGA GGTATTCCAT GATCCTATTT TGACCGCCGC ATTGACGGAT CGCTTGACGC ATAAATCGCA CGTGGTGAAT ATGATCGGCC CATCTTACCG 9500
AATGAGAGAA ACGCAAAAGT GGCTTGAAAA TAGCCATTCA TAAGTGGCTC AAAATTAAAT TGCGAAATGG CTCAATTTTC AGTTGCGAAA TACA

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
istA ISSau9.1 319-1656 Transposase   +
istB ISSau9 1631-2401 Accessory Gene ATPase Transposition Helper +
cfrA (ARO:3003441) TnSau9 2944-3993 Passenger Gene Antibiotic Resistance +
lsaB (ARO:3003111) TnSau9 5500-6978 Passenger Gene Antibiotic Resistance +
istA ISSau9 7461-8798 Transposase   +
istB ISSau9 8773-9543 Accessory Gene ATPase Transposition Helper +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
istA IstA ISSau9.1 1338 319-1656 +
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   Has two amino acid changes relative to istA (ISSau9)
Protein Sequence:  
 MTEEIVERLK GFIQDNEWKR KNNMSKQQMK KIDMYEKLQE ERYDIGYTTV RNFVNSEERR TKEVYIRQRY SAAWEVEFDW GEVKLVIDGK QKSYSLAVFT
LAYSNYRFAL LYESETMICI QDAHTKLIDH LGFVPSVFTY DNMRTVVKSF VGTDRKITDG MINLSNYYEF RIRLCEPRKG NQKGHVERSV EVVRRKAFSY
DIAFPSLEDA QKRLAQTILS LNKRIHHEKK LAHIELKDQE KVAAAQGALP LPFDVSEVVE CRVDKYSTVT IKQNRYSVPE GHVGAWIRAK VSADSVRLFI
EGEFVAEHRR NWGVHQWEMD LYHYIKTFTK KKGALAQSEC LSQAPNQIKK LFENRYIGKE KDFLELLLYV REKNQWDNVM AAVRQIETKG LGDITTEKLI
FLSEQTDLVP AVPLHPDETV EQALQNIKAF SAMFNQVDEG VLEHG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
istB IstB ISSau9 771 1631-2401 +
Class:   Accessory Gene
Sub Class:   ATPase Transposition Helper
Protein Sequence:  
 MKECLNMDKK QSVIDLCKEL RLPGIRELVE EDVAFQETTP AIDLLHTVLT KEKNDRWIRS KSTRIRRANF PEKKLLEEID TRALPTDAQQ KLPNLQTLDF
IKAGQNVILT GSPGTGKSHL AIGLGVEACL AGYKVYFASV SSLINQLKES RSERTLRSFE LRFEKYDLVI IDELGYISFD KEGAELLFTH LSLRAGRSST
IITSNLSFAK WEEVFHDPIL TAALTDRLTH KSHVVNMIGP SYRMRETQKW LENSHS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
cfrA (ARO:3003441) CfrA TnSau9 1050 2944-3993 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic target alteration (ARO:0001001)
Target:   oxazolidinone antibiotic (ARO:3000079)||streptogramin antibiotic (ARO:0000026)||pleuromutilin antibiotic (ARO:3000670)||phenicol antibiotic (ARO:3000387)||lincosamide antibiotic (ARO:0000017)||streptogramin A antibiotic (ARO:0000034)
Sequence Family:  Cfr 23S ribosomal RNA methyltransferase (ARO:3000202)
Comment:   perfect match to reference sequence for ARO:3003441
Protein Sequence:  
 MNFNNKTKYG KIQEFLRSNN EPDYRIKQIT NAIFKQRISR FEDMKVLPKL LREDLINNFG ETVLNIKLLA EQNSEQVTKV LFEVSKNERV ETVNMKYKAG
WESFCISSQC GCNFGCKFCA TGDIGLKKNL TVDEITDQVL YFHLLGHQID SISFMGMGEA LANRQVFDAL DSFTDPNLFA LSPRRLSIST IGIIPSIKKI
TQEYPQVNLT FSLHSPYSEE RSKLMPINDR YPIDEVMNIL DEHIRLTSRK VYIAYIMLPG VNDSLEHANE VVSLLKSRYK SGKLYHVNLI RYNPTISAPE
MYGEANEGQV EAFYKVLKSA GIHVTIRSQF GIDIDAACGQ LYGNYQNSQ

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
lsaB (ARO:3003111) LsaB TnSau9 1479 5500-6978 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic target protection (ARO:0001003)
Target:   lincosamide antibiotic (ARO:0000017)||pleuromutilin antibiotic (ARO:3000670)||streptogramin antibiotic (ARO:0000026)
Sequence Family:  lsa-type ABC-F protein (ARO:3004472)
Comment:   ATPase component of ABC transporters with duplicated ATPase domains||strict match to reference sequence for ARO:3003111 (bitscore: 1006)
Protein Sequence:  
 MSMIHVQNLT FSYPSSFDNI FEDVSFQIDT DWKLGFIGRN GRGKTTLFNL LLDKFEYRGK IISSVDFNYF PYPVEDKSKY THEILEEICP QAEDWEFLRE
IAYLNVDAEA MYRPFETLSN GEQTKVLLVA LFLNEGQFLL IDEPTNHLDT EARKTVSNYL RKKKGFILIS HDRNFLDGCV DHILSINRAD IEVQSGNYSS
WKLNFDRQQG HEQATNERLQ KDIGRLEQST KRSAGWSNRV EASKNGTTNS GSKLDKGFVG HKAAKMMKRS KNLEARQQKS IEEKSKLLKN IEKTESLQFE
PVEYKSKELI QLTDVSVIYD GQVVNKPISF NVEQGDRIVL DGKNGSGKSS ILKLILGDPI QYTGTLNTGS NLITSYVQQD TSHLKGMLAD FIEENEIDES
LFKAILRKLD FDRVQFEKDI SHYSGGQKKK LLIAKSLCEK AHLYIWDEPL NFIDIYSRMQ IEELIQTFNP TMVFVEHDQT FQETISTKII KI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
istA IstA ISSau9 1338 7461-8798 +
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
 MTEEIVERLK GFIQDNEWKR KNNMSKQQMK KIDMYEKLQE EGYDIGYTTV RNFVNSEERR TKEVYIRQRY SAAWEVEFDW GEVKLVIDGK QKSYSLAVFT
LAYSNYRFAL LYESETMICI QDAHTKLIDH LGFVPSVFTY DNMRTVVKSF VGTDRKITDG MINLSNYYEF RIRLCEPRKG NQKGHVERSV EVVRRKAFSY
DIAFPSLEDA QKRLAQTILS LNKRIHHEKK LAHIELKDQE KVAAAQEALP LPFDVSEVVE CRVDKYSTVT IKQNRYSVPE GHVGAWIRAK VSADSVRLFI
EGEFVAEHRR NWGVHQWEMD LYHYIKTFTK KKGALAQSEC LSQAPNQIKK LFENHYIGKE KDFLELLLYV REKNQWDNVM AAVRQIETKG LGDITTEKLI
FLSEQTDLVP AVPLHPDETV EQALQNIKAF SAMFNQVDEG VLEHG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
istB IstB ISSau9 771 8773-9543 +
Class:   Accessory Gene
Sub Class:   ATPase Transposition Helper
Protein Sequence:  
 MKECLNMDKK QSVIDLCKEL RLPGIRELVE EDVAFQETTP AIDLLHTVLT KEKNDRWIRS KSTRIRRANF PEKKLLEEID TRALPTDAQQ KLPNLQTLDF
IKAGQNVILT GSPGTGKSHL AIGLGVEACL AGYKVYFASV SSLINQLKES RSERTLRSFE LRFEKYDLVI IDELGYISFD KEGAELLFTH LSLRAGRSST
IITSNLSFAK WEEVFHDPIL TAALTDRLTH KSHVVNMIGP SYRMRETQKW LENSHS

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
ISSau9.1-AM408573 ISSau9.1 Insertion Sequence 1-2452 2452
ISSau9-AM086211 ISSau9 Insertion Sequence 7143-9594 2452

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
IRR ISSau9 2426-2452 ACCGAGTTAA AAGTCAACGC TTTATGT
IRL ISSau9 7143-7169 TGTATATGTT AACTTAAAAC TGAGCCA

 References     

Kehrenberg C, Aarestrup FM, Schwarz S. IS21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr. Antimicrob Agents Chemother. 2007 Feb;51(2):483-7. doi: 10.1128/AAC.01340-06. Epub 2006 Dec 4. PubMed ID: 17145796