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Internal Transposable Elements | |
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| Name: TnPfu1 (Synonyms: Tn7220) |
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| Family: Tn402 |
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| Evidence of Transposition: Yes |
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| Host |
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| Host Organism: | Pseudomonas fulva KUN1351 | Molecular Source: | chromosome |
| Place of Origin: | Kyoto, Japan | Date of Isolation: | 2018 |
| | Other Geographic Information: | lung cancer patient Kyoto University Hospital |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TGTCATTTTC AGAAGACGAC TGCACCAATT GATTGGGCGT AATGGCTGTT GTGCAGCCAG CTCCTGACAG TTCAATATCA GAAGTGATCT GCACCAATCT 100
CGACTATGCT CAATACTCGT GTGCACCAAA GCGAGGTGAG CATGGCGACG GACACCCCAC GGATTCCAGA ACAAGGCGTG GCCACTCTGC CTGATGAGGC 200
TTGGGAGCGT GCGCGCCGTC GTGCGGAGAT CATCAGTCCG TTGGCGCAGT CGGAGACGGT CGGGCACGAA GCGGCCGATA TGGCGGCTCA GGCGCTGGGC 300
TTGTCTCGGC GCCAGGTATA CGTTCTGATC CGGCGTGCCC GGCAAGGCAG CGGCCTCGTG ACGGATCTGG TGCCCGGCCA GTCCGGTGGA GGTAAAGGTA 400
AGGGGCGCTT GCCGGAACCG GTCGAGCGCG TCATCCACGA GCTACTGCAA AAGCGGTTCC TGACCAAGCA GAAGCGCAGC CTAGCGGCCT TTCACCGCGA 500
AGTCACTCAG GTGTGCAAGG CTCAAAAACT GCGAGTGCCG GCGCGCAATA CCGTGGCCTT ACGGATCGCT AGCCTTGACC CGCGCAAGGT CATCCGCCGG 600
CGGGAAGGCC AGGATGCCGC TCGTGACCTA CAAGGTGTGG GCGGCGAGCC TCCTGCCGTG ACCGCGCCGC TGGAGCAGGT GCAGATAGAC CATACGGTCA 700
TCGACCTGAT CGTGGTCGAT GACCGCGACC GGCAACCTAT TGGCCGCCCG TACCTGACCC TCGCCATCGA CGTGTTCACC CGCTGCGTGC TCGGCATGGT 800
CGTCACGCTG GAAGCGCCGT CTGCCGTTTC GGTTGGCCTG TGCCTCGTGC ATGTCGCCTG CGACAAGCGC CCTTGGCTGG AAGGACTGAA CGTGGAAATG 900
GATTGGCAGA TGAGCGGCAA GCCCTTGCTG CTCTACCTAG ACAACGCGGC CGAGTTCAAG AGCGAGGCCC TGCGCCGGGG TTGCGAGCAG CATGGCATCC 1000
GGCTGGACTA TCGCCCGCTG GGACAGCCGC ACTATGGCGG CATCGTGGAA CGGATCATCG GCACGGCGAT GCAGATGATT CACGACGAAC TGCCGGGAAC 1100
GACCTTCTCC AACCCTGACC AGCGCGGCGA CTACGATTCC GAAAACAAGG CCGCCCTGAC GCTGCGCGAG CTAGAGCGCT GGCTCACATT GGCGGTCGGC 1200
ACCTACCACG GTTCGGTGCA CAACGGCCTG CTCCAACCGC CGGCCGCGCG CTGGGCCGAG GCCGTGGCGC GTGTCGGCGT ACCGGCCGTC GTCACACGCG 1300
CTACTTCGTT CCTGGTCGAT TTTCTGCCGA TCCTCCGGCG CACGCTGACC CGCACCGGCT TTGTCATCGA CCACATCCAC TACTACGCCG ATGCGCTCAA 1400
GCCGTGGATT GCGCGGCGTG AACGCTGGCC GTCCTTTCTG ATCCGGCGCG ATCCGCGCGA CATCAGCCGT ATCTGGGTCC TGGAACCGGA GGGACAGCAT 1500
TACCTGGAAA TTCCCTACCG TACCTTGTCG CATCCGGCTG TCACCCTCTG GGAACAACGG CAGGCGCTGG CGAAACTGCG GCAGCAAGGG CGCGAACAGG 1600
TGGATGAGTC GGCGCTGTTC CGCATGATCG GCCAGATGCG TGAGATTGTG ACCAGCGCGC AGAAGGCCAC ACGCAAGGCG CGGCGTGACG CGGATCGCCG 1700
CCAGCACCTC AAGACATCAG CTCGGCCGGA CAAGCCCGTT CCGCCGGATA CGGATATTGC CGACCCGCAG GCAGACAACT TGCCACCCGC CAAACCGTTC 1800
GACCAGATTG AGGAGTGGTA GCCGTGGACG AATATCCCAT CATCGACCTG TCCCACCTGC TGCCGGCGGC GCAGGGGCTG GCTCGGCTGC CGGCGGACGA 1900
GCGCGTCCAG CGCCTTCGCG CCGACCGCTG GATCGGCTAT CCGCGCGCGG TCGAGGCGCT GAACCGGCTG GAAGCCCTTT ATGCGTGGCC AAACAAGCAA 2000
CGCATGCCCA ACCTGCTGCT GGTCGGCCCT ACCAACAATG GCAAGTCGAT GATCGTCGAG AAGTTCCGCC GCACCCACCC GGCCAGCGCC GACGCCGACC 2100
AGGAGCACAT CCCAGTGTTG GTCGTGCAGA TGCCGTCCGA GCCGTCCGTG ATCCGCTTCT ACGTCGCGCT GCTCGCCGCG ATGGGCGCGC CGCTGCGCCC 2200
ACGCCCACGG TTGCCGGAAA TGGAGCAACT GGCTCTGGCA CTGCTGCGCA AGGTCGGTGT GCGTATGCTG GTGATCGACG AACTGCACAA CGTCCTGGCC 2300
GGCAATAGCG TCAACCGCCG GGAATTTCTC AACCTGCTGC GCTTTCTCGG CAACGAGCTG CGCATCCCGC TGGTCGGGGT TGGCACACGC GACGCCTACC 2400
TGGCCATTCG TTCGGACGAT CAGTTGGAGA ACCGGTTCGA GCCGATGCTG CTGCCGGTAT GGGAGGCCAA CGACGATTGC TGCTCACTGC TGGCCAGCTT 2500
CGCGGCTTCG CTCCCGCTGC GGCGACCCTC GTCGATTGCC ACGCTGGATA TGGCTCGCTA CCTGCTCACG CGCAGCGAGG GCACCATCGG CGAACTGGCG 2600
CACCTGTTAA TGGCGGCGGC TGTCGCTGCC GTGGAAAGTG GTGAGGAAGC GATCAACCAT CGCACGCTCA GCATGGCCGA CTACACCGGC CCCAGCGAGC 2700
GGCGACGACA GTTCGAGCGG GAACTAATGT GAAGCCCGCG CCGCGCTGGC CGCTGCATCC CGCTCCGAAA GAAGGCGAGG CGCTGTCCTC ATGGCTCAAC 2800
CGCGTGGCCC TTTGCTATCA CATGGAACTG CCCGAGCTGC TGGAGCACGA TCTTGGCCAC AGCCAGGTCG ATGACCTGGA CACCGCGCCA CCACTGTCGC 2900
TGCTGGCGAT GCTTTCCCAG CGGAGCGGGA TCGATCCGGA CCGGCTGCGC AGCATGAGTT TCGCCGGCTG GGTGCCTTGG CTACTGGATA GTCTTGATGA 3000
CCAGATTCCA GACGCATTGG AAACCTATGC ATTTCAGCTC TCGGTACTGC TGCCAAAACT CCACCGTAAG AAGCGATCCA TCACGAGGTG GCGTGCCTGG 3100
CTACCCAGCC AGCCGATACA CTGTGCCTGT CCGCTCTGCC TGAGCGATCC GGATAACCAA GCCGTACTGC TCGCGTGGAA GCTGCCCCTG ATGCTGAGCT 3200
GCCCGCTGCA TGGCTGCTGG CTGGAATCCT ATTGGGGAGT GCCTGGGCGG TTTCTCGGCT GGGAGAACGC CGACGCCGAA CCGCGCACCG CCAGCGACGC 3300
GATTGCGGCG ATGGATCAGC GTACCTGGCA GGCACTGACA ACCGGTCACG TGGAGCTGCC GTGCCGACGC ATCCACGCCG GATTGTGGTT TCGACTTCTT 3400
CGCACGCTGC TCGATGAGCT GAACACCCCG CTTTCCGCGT GCGGAACCTG CGCGGGGTAT CCCCGCCAAG TCTGGGAAGG CTGCGGGCAT CCGCTGCGTG 3500
CTGGGCAAAG TCTGTGGCGA CCGTATGAAA CCCTAAATCC GGTAGTACGG TTACAGATGC TGGAGGCGGC GGCAACGGCA ATCAGCTTGA TTGAGGTGAG 3600
GGACATCAGC CCGCCAGGCG AGCAGGCAAA GCTATTCTGG TCCGAGCCCC AAACCGGGTT CACCAGTGGC CTGCCGACGA AAGCGCCGAA GCCCGAGCCC 3700
ATCAATCACT GGCAGCGTGC AGTCCAGGCC ATCGACGAGG CCATCATTGA AGCGCGACAC AACCCCGAGA CGGCACGCTC GCTGTTCGCG TTGGCTTCCT 3800
ATGGTCGGCG CGATCCCGCT TCCTTGGAAC GGTTGCGCGC CACCTTCGTG AAGGAAGGCA TCCCGCCGGA ATTTCTGTCA CATTACCTGC CTGATGCACC 3900
CTTTGCATGT CTTAAACAAA ATGACGGGTT AAGTGACAAA TTTTGACGGA TAGAGCTTTC CGGCTCACAC TGTCACATAA TCGAACGTAT ACGTGACAGG 4000
GGGCGCAATG TTGATCGGCT ACATGCGAGT ATCGAAAGCG GATGGATCCC AATCCACCGA TCTGCAGCGC GATGCACTCA TCGTCGCCGG CGTGAGTCCT 4100
GCACATCTCT ACGAGGATAT GGCTTCAGGC CGACGCGATG ATCGACCGGG GTTGGCTGCT TGCCTGAAGG CGCTTCGTGA AGGTGACACG CTGATCGTGT 4200
GGAAGCTTGA TCGGCTCGGC CGCGATCTGC GCCACCTGAT CAATACCGTT CACGACCTGA CAGCGCGTAG CGTGGGTTTG AAGGTCCTGG CCGGTCATGG 4300
TGCGGCGGTC GATACGACGA CTGCCGCCGG CAAGCTGGTG TTCGGTATTT TTGCTGCGCT GGCCGAATTC GAGCGGGAGT TGATTTCCGA GCGAACAGTC 4400
GCCGGGCTCG TCTCGGCGCG CGCTCGCGGC AGGAAAGGAG GGCGCCCCTT CAAGATGACC GCCACCAAGC TACGCCTGGC GATGGCCAGC ATGGGGCAAC 4500
CGGAAACCAA GGTGGGCAAT CTCTGCGAAG AACTAGGGAT TACCCGGCAG ACGCTCTACC GGCATGTGTC GCCCAAGGGC GAATTGCGGC CGGACGGCGT 4600
AAAGCTGCTC TCCCGCGGAT CAGCCGCATA AATGGTGGCG ACTTGGAACG GGGCGCTGTT TAGTGCGGCA ACGATCCGAC AGCCGGTGTC GACCCTGAGC 4700
CGCCATCCAG ATGAAAGCAC CACAACCGTC ATTCAGCCGG GCGACAAGTG CAAGGCCAAG GCGTCCAGCG GGCTGGAGGT GCCTCCGGCA GCGACTTTCA 4800
GCACATGCGT GTAGATCATC GTCGTGCTCA CGTCCGAATG CCCCAACAAC TCTTGCACCG TTCGGATGTC TGTGCCTGCT TGCAGCAAGT GGGTGGCGAA 4900
TGAGTGGCGC AGGGTGTGGA CAGATACGTG TTTGGCAATG CCAGCCTGAA CTACCGCTTT TTTTAGTTGC CGGTTCAGTC TTTCCTCAAA CAAGTGGTGG 5000
CGGCGCTCAA CGCCGGTTTG TGGGTCCACA GACAGCTTGG CCGATGGAAA CACCCAGAAC CAGGCCCAGC TCTCGCCCGC CCTGGGGTAC TTGCGCTCCA 5100
GTGCATGAGG CAGATACACG CCTCCGCGCC CCGTGGCACG GTCCTGCCCC CACACAGCGC GGACCTGAAT CAGCTGCGCC CGCAACCGAG GTACGAGCGC 5200
CCTGGGCAGC ATCACCACGC GGTCCTTGTC GCCCTTGCCG CTGCGCACAA TGATCGCGTG GCGGTCGAAA TCCACATCCT TGACCCGCAG GCCCAGCGCT 5300
TCGCGCAGGC GCAACCCACT GCCGTAAAGC AGGGCGGCCA ACAGCGCTTC GGTGCCCGCC ATGTGCGAAA GCAACGTCTG AACCTCCTGC ACCGTCAGCA 5400
CCACCGGAAT CCGCTTGCGT TCTGGCGGCC GACCAATCTG CTGCATCCAC GGCAATTCCA TGCCCAGCAC CTGCCGATAC AAGAACAACA GCGCGTTGAG 5500
CGCCTGCCGG TGGGTGGCCG GCGCCACTTG CTTCTCGGTG GCGAGCATGG TCAGAAAACC CTCGACTTCA GCTTGCCCCA TTTCGCGCGG ATGTCGAAAC 5600
CCACCATGGC TGCGGGCCGT CCACAACACA AATGCCTTGG CCCAGTAGAC ATAAGCCTTC TCGGTCTGTA GGCTGTAATG CAGGTAGCGA ACCCGTTCGC 5700
GTACCTGATC GAGCAGCTTG ATGGACCGGG GAGGGACCCA GTCAGGTTTG GCAGATCCGT TATACCTGTT CATGCATCCA GTATGATGCT TTACAGGATT 5800
GATTTCAAAC ACTTTTTTTG GGTGCCGTGC GACTTTGTTT AACGACCACG GTTGTGGGTA TCCGGTGTTT GGTCAGATAA ACCACAAGTT AGGCAGCACA 5900
AAGACCGTTC TCATGACCAC TACCATTAGC TTCGTCACGC TGCGCCTCAT GACCGAGCAC GACCTTCCGA TGCTCCATGA CTGGCTAAAT CGGCCTCACA 6000
TCGTTGAGTG GTGGGGCGGA GAAGAAACAC GTCCAACACT TGCTGAAGTG CTGGAGCAAT ACCTACCAAG CGCCCTGGCG AAAGAGTCCG TCACTCCCTA 6100
CATCGCAATG CTGGATGAAG AACCGATTGG GTACGCTCAG TCGTACATTG CACTCGGAAG CGGTGACGGA TGGTGGGAAG ACGAAACCGA TCCAGGAGTA 6200
CGCGGAATAG ACCAGTTTCT GGCGAATCCA TCGCAGCTGG GCAAGGGCTT GGGAACCAAG CTCGTTTGCG CGCTCGTTGA GATGCTGTTC AAAGACGCTG 6300
AGGTAACCAA GATCCAAACG GACCCGTCGC CGAACAACTT ACGCGCAATC CGGTGCTACG AGAAGGCGGG TTTTGTGGCG CAAAGAACCA TAAACACCCC 6400
AGATGGACCG GCCGTATACA TGGTTCAAAC ACGTCAGGCG TTCGAGCAGG CGCGCAGTGC TGTCTAACCC TTCCATCGAG GGGGACGTCC AAGGGCTATC 6500
GTCCTTGGCC GCCCCTCATG TCAAACGTTA GCGCCCATGG AAGGTATCAG CCACATCACG CTTATTGTCC GCGACCTCTC GCGCATGACC ACCTTCCTTT 6600
GCGATGGTCT CGGTGCGCGA GAGGTTTATG ACAGTGCTGG CCACAATTAC TCGCTTTCCC GCGAGAAATT CTTTGTCCTT GGTGGCGTTT GGTTGGCCGC 6700
TATGGAGGGA GTGCCGCCAT CTGAGCGCTC CTATCAGCAT GTCGCCTTTC GGGTGAGTGA GTCAGATCTT GCCGTATATC AGGCAAGACT TGGGTCGCTG 6800
GGCGTGGAGA TTCGCCCACC CAGGCCACGC GTGAATGGAG AGGGGCTGTC CCTGTACTTC TATGATTTTG ACAACCATCT GTTTGAGCTG CACACCGGCA 6900
CATTGGAGCA GCGCCTTGCC AGGTACGGAG CTGGGCGCTA ACAATTCATT CAAGCCGAAG CCGCTTCGCG GCTCGGCTTA ATTCAGGCGT TAGAAAAGGA 7000
AAAGTATGAG CAAGTTATCT GTATTCTTTA TATTTTTGTT TTGCAGCATT GCTACCGCAG CAGAGTCTTT GCCAGATTTA AAAATTGAAA AGCTTGATGA 7100
AGGCGTTTAT GTTCATACTT CGTTTGAAGA AGTTAACGGG TGGGGCGTTG TTCCTAAACA TGGTTTGGTG GTTCTTGTAA ATGCTGAGGC TTACCTAATT 7200
GACACTCCAT TTACGGCTAA AGATACTGAA AAGTTAGTCA CTTGGTTTGT GGAGCGTGGC TATAAAATAA AAGGCAGCAT TTCCTCTCAT TTTCATAGCG 7300
ACAGCACGGG CGGAATAGAG TGGCTTAATT CTCGATCTAT CCCCACGTAT GCATCTGAAT TAACAAATGA ACTGCTTAAA AAAGACGGTA AGGTTCAAGC 7400
CACAAATTCA TTTAGCGGAG TTAACTATTG GCTAGTTAAA AATAAAATTG AAGTTTTTTA TCCAGGCCCG GGACACACTC CAGATAACGT AGTGGTTTGG 7500
TTGCCTGAAA GGAAAATATT ATTCGGTGGT TGTTTTATTA AACCGTACGG TTTAGGCAAT TTGGGTGACG CAAATATAGA AGCTTGGCCA AAGTCCGCCA 7600
AATTATTAAA GTCCAAATAT GGTAAGGCAA AACTGGTTGT TCCAAGTCAC AGTGAAGTTG GAGACGCATC ACTCTTGAAA CTTACATTAG AGCAGGCGGT 7700
TAAAGGGTTA AACGAAAGTA AAAAACCATC AAAACCAAGC AACTAAATTT CTAACAAGTC GTTGCAGCAC GCCACTACGT GGCTGGACAG TTTGTAAGTT 7800
GCGCTTTTGT GGTTTGCTTC GCAAAGTATT CCACAACGCG CAACTTACAA ACTGCCGCTG AACTTAGCGT TGAGCGACTG CTTACCCGTT TGACGGTCGG 7900
CCGCTCCTGG CCGGCTGCTG CCCGCCATTG CATGATGCCC CCCCATCCGC TGCCGTAGTG CAGTCGGCTT CTGACGTTCA GTGCAGCCGC CTTCTGAAAA 8000
CGACA
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| Recombination Sites |
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| Name |
Coordinates |
Gene |
Sequence |
| r6 |
3824-3837 |
14 |
TTGGAACGGT TGCG |
| r5 |
3870-3883 |
14 |
AATTTCTGTC ACAT |
| r4 |
3875-3888 |
14 |
CTGTCACATT ACCT |
| r3 |
3926-3939 |
14 |
GGGTTAAGTG ACAA |
| res |
3967-4000 |
34 |
ACACTGTCAC ATAATCGAAC GTATACGTGA CAGG |
| r2 |
3970-3983 |
14 |
CTGTCACATA ATCG |
| r1 |
3986-3999 |
14 |
CGTATACGTG ACAG |
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tniA |
TnPfu1 |
142-1821 |
Transposase |
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+ |
| tniB |
TnPfu1 |
1824-2732 |
Accessory Gene |
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+ |
| tniQ |
TnPfu1 |
2729-3946 |
Accessory Gene |
Target Site Selection |
+ |
| tniR |
TnPfu1 |
4008-4631 |
Accessory Gene |
Resolvase |
+ |
| intI3 |
TnPfu1 |
4733-5773 |
Integron Integrase |
Class 3 |
- |
| AAC(6')-31 (ARO:3002585) |
TnPfu1 |
5949-6467 |
Passenger Gene |
Antibiotic Resistance |
+ |
| FosX (ARO:3000198) |
TnPfu1 |
6585-6941 |
Passenger Gene |
Antibiotic Resistance |
+ |
| bla IMP-1 (ARO:3002192) |
TnPfu1 |
7006-7746 |
Passenger Gene |
Antibiotic Resistance |
+ |
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniA |
TniA |
TnPfu1 |
1680 |
142-1821 |
+ |
| Class: | Transposase |
| Transpoase Chemistry: | DDE |
| Comment: | can be extended upstream by 12 amino acids| identical to tniA (Tn1721 and In2)| 25% amino acid sequence identity to TnsB from Tn7 |
Protein Sequence:
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MATDTPRIPE QGVATLPDEA WERARRRAEI ISPLAQSETV GHEAADMAAQ ALGLSRRQVY VLIRRARQGS GLVTDLVPGQ SGGGKGKGRL PEPVERVIHE
LLQKRFLTKQ KRSLAAFHRE VTQVCKAQKL RVPARNTVAL RIASLDPRKV IRRREGQDAA RDLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDDRDRQPI
GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLVHVAC DKRPWLEGLN VEMDWQMSGK PLLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG
IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAVARVGV PAVVTRATSF LVDFLPILRR
TLTRTGFVID HIHYYADALK PWIARRERWP SFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR
EIVTSAQKAT RKARRDADRR QHLKTSARPD KPVPPDTDIA DPQADNLPPA KPFDQIEEW
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniB |
TniB |
TnPfu1 |
909 |
1824-2732 |
+ |
| Class: | Accessory Gene |
| Sequence Family: | ATP binding protein? |
| Comment: | similar function to Tn7 tnsC and MuB |
Protein Sequence:
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MDEYPIIDLS HLLPAAQGLA RLPADERVQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASAD ADQEHIPVLV
VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ
LENRFEPMLL PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAAVAAV ESGEEAINHR TLSMADYTGP SERRRQFERE
LM
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniQ |
TniQ |
TnPfu1 |
1218 |
2729-3946 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Target Site Selection |
| Comment: | similar function to Tn7 tnsC and MuB |
Protein Sequence:
|
VKPAPRWPLH PAPKEGEALS SWLNRVALCY HMELPELLEH DLGHSQVDDL DTAPPLSLLA MLSQRSGIDP DRLRSMSFAG WVPWLLDSLD DQIPDALETY
AFQLSVLLPK LHRKKRSITR WRAWLPSQPI HCACPLCLSD PDNQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADAEPRTASD AIAAMDQRTW
QALTTGHVEL PCRRIHAGLW FRLLRTLLDE LNTPLSACGT CAGYPRQVWE GCGHPLRAGQ SLWRPYETLN PVVRLQMLEA AATAISLIEV RDISPPGEQA
KLFWSEPQTG FTSGLPTKAP KPEPINHWQR AVQAIDEAII EARHNPETAR SLFALASYGR RDPASLERLR ATFVKEGIPP EFLSHYLPDA PFACLKQNDG
LSDKF
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniR |
TniR |
TnPfu1 |
624 |
4008-4631 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Resolvase |
| Transpoase Chemistry: | Serine |
| Sequence Family: | Serine Site-Specific Recombinase |
| Comment: | also called tniR |
Protein Sequence:
|
MLIGYMRVSK ADGSQSTDLQ RDALIVAGVS PAHLYEDMAS GRRDDRPGLA ACLKALREGD TLIVWKLDRL GRDLRHLINT VHDLTARSVG LKVLAGHGAA
VDTTTAAGKL VFGIFAALAE FERELISERT VAGLVSARAR GRKGGRPFKM TATKLRLAMA SMGQPETKVG NLCEELGITR QTLYRHVSPK GELRPDGVKL
LSRGSAA
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| intI3 |
IntI3 |
TnPfu1 |
1041 |
4733-5773 |
- |
| Class: | Integron Integrase |
| Sub Class: | Class 3 |
| Transpoase Chemistry: | Tyrosine |
| Sequence Family: | Class 3 Integron Tyrosine Integrase |
Protein Sequence:
|
MNRYNGSAKP DWVPPRSIKL LDQVRERVRY LHYSLQTEKA YVYWAKAFVL WTARSHGGFR HPREMGQAEV EGFLTMLATE KQVAPATHRQ ALNALLFLYR
QVLGMELPWM QQIGRPPERK RIPVVLTVQE VQTLLSHMAG TEALLAALLY GSGLRLREAL GLRVKDVDFD RHAIIVRSGK GDKDRVVMLP RALVPRLRAQ
LIQVRAVWGQ DRATGRGGVY LPHALERKYP RAGESWAWFW VFPSAKLSVD PQTGVERRHH LFEERLNRQL KKAVVQAGIA KHVSVHTLRH SFATHLLQAG
TDIRTVQELL GHSDVSTTMI YTHVLKVAAG GTSSPLDALA LHLSPG
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| AAC(6')-31 (ARO:3002585) |
AAC(6')-31 |
TnPfu1 |
519 |
5949-6467 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Antibiotic Resistance |
| Function: | antibiotic inactivation (ARO:0001004) |
| Transpoase Chemistry: | aminoglycoside N(6')-acetyltransferase |
| Target: | aminoglycoside antibiotic (ARO:0000016) |
| Sequence Family: | AAC(6') (ARO:3000345) |
| Comment: | 99% identity to reference sequence ARO:3002585 (bit score: 355) |
Protein Sequence:
|
MTEHDLPMLH DWLNRPHIVE WWGGEETRPT LAEVLEQYLP SALAKESVTP YIAMLDEEPI GYAQSYIALG SGDGWWEDET DPGVRGIDQF LANPSQLGKG
LGTKLVCALV EMLFKDAEVT KIQTDPSPNN LRAIRCYEKA GFVAQRTINT PDGPAVYMVQ TRQAFEQARS AV
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| FosX (ARO:3000198) |
FosX |
TnPfu1 |
357 |
6585-6941 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Antibiotic Resistance |
| Function: | antibiotic inactivation (ARO:0001004) |
| Target: | fosfomycin (ARO:0000025) |
| Sequence Family: | fosfomycin thiol transferase (ARO:3000133) |
| Comment: | loose match to reference sequence for ARO:3000198 (53% identity, bitscore: 142) |
Protein Sequence:
|
MTTFLCDGLG AREVYDSAGH NYSLSREKFF VLGGVWLAAM EGVPPSERSY QHVAFRVSES DLAVYQARLG SLGVEIRPPR PRVNGEGLSL YFYDFDNHLF
ELHTGTLEQR LARYGAGR
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| bla IMP-1 (ARO:3002192) |
Bla IMP-1 |
TnPfu1 |
741 |
7006-7746 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Antibiotic Resistance |
| Function: | antibiotic inactivation (ARO:0001004) |
| Target: | cephalosporin (ARO:0000032)||penem (ARO:3003706)||penam (ARO:3000008)||cephamycin (ARO:0000044)||carbapenem (ARO:0000020) |
| Sequence Family: | IMP beta-lactamase (ARO:3000020) |
| Comment: | 100% identical to reference sequence for ARO:3002192 |
Protein Sequence:
|
MSKLSVFFIF LFCSIATAAE SLPDLKIEKL DEGVYVHTSF EEVNGWGVVP KHGLVVLVNA EAYLIDTPFT AKDTEKLVTW FVERGYKIKG SISSHFHSDS
TGGIEWLNSR SIPTYASELT NELLKKDGKV QATNSFSGVN YWLVKNKIEV FYPGPGHTPD NVVVWLPERK ILFGGCFIKP YGLGNLGDAN IEAWPKSAKL
LKSKYGKAKL VVPSHSEVGD ASLLKLTLEQ AVKGLNESKK PSKPSN
|
|
| Internal Repeat Elements |
|
|
| Name |
Associated Mobile Element |
Coordinates |
Sequence (Top Strand) |
| repeat t1 |
TnPfu1 |
9-27 |
TCAGAAGACG ACTGCACCA |
| repeat t2 |
TnPfu1 |
49-67 |
AACACGTCGG TCGAGGACT |
| repeat t3 |
TnPfu1 |
78-97 |
TCAGAAGTGA TCTGCACCAA |
| repeat t4 |
TnPfu1 |
110-128 |
TCAATACTCG TGTGCACCA |
| repeat i3 |
TnPfu1 |
7916-7935 |
ACGACGGGCG GTAACGTACT |
| repeat i2 |
TnPfu1 |
7956-7974 |
ATCACGTCAG CCGAAGACT |
| IRi |
TnPfu1 |
7973-8005 |
CTGCAAGTCA CGTCGGCGGA AGACTTTTGC TGT |
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| References |
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Yamamoto M, Matsumura Y, ORCID: 0000-0001-8595-8944, Gomi R, ORCID: 0000-0002-3299-333X, Matsuda T, Nakano S, Nagao M, Tanaka M, Ichiyama S. Molecular Analysis of a bla(IMP-1)-Harboring Class 3 Integron in Multidrug-Resistant Pseudomonas fulva. Antimicrob Agents Chemother. 2018 Jul 27;62(8):e00701-18. doi: 10.1128/AAC.00701-18. Print 2018 Aug. PubMed ID: 29784850
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