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 References  
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Transposon
Name: TnPa38       (Synonyms: Tn7172)
Family: Tn3        Group: Tn21
Evidence of Transposition: no
 Host     

Host Organism:Pseudomonas aeruginosa DK2
Place of Origin:Denmark Date of Isolation:2012
Other Geographic Information:Copenhagen CysticFibrosis Clinic CF patients since 1973

 Map     



 Terminal Inverted Repeats (IR)     

IRL (Length: 38 bp)GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAG
IRR (Length: 41 bp)GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGCCG

 Sequence     
DNA SequenceLength  6455 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAGCG TGCGTGGAGG CTGGCACCCA GCGGTACAGC GTCGGAATGG ACACGCCGAG GTTCTTGGCC 100
ACGTCCTTGG GCGGCACCCC GCTGGCCAGC AGCTTCTTGG CCGACTCGAT CTTGCTGTCG GTCATCTTCG GCTTGCGGCC GCCTTTGCGG CCGAGCTGCT 200
TGGCGACTTC CAGCCCGGCG CGGGTGCGCT CGACGGTCAG CTCGCGCTCC ATTTCGGCAA GGCTCGCCAT GACGTGGAAG AAGAACCGCC CGGATGGTGT 300
GCCGGTGTCG ATGGAGTCGG TGAGGCTCCT GAACTGGACA CCGTGCTTGT GCAGATCGCC GACCAGATCG ACCAGTTGCT TGACCGACCG GCCCAGCCGG 400
TCGAGCTTCC AGACGACCAA AGTATCGCCT TCGCGCAGCA TTTCGAGCGT CTTGGCCAAG CCAGGCCGGT CTGCCCGCGT GCCACTCACC TTGTCCTCGA 500
AGACCTTTTT ACATCCGGCC TTGCTCAAGG CTTCGCGTTG CAGCTCCAGG TTCTGATCCT GCGTCGAGAC GCGCGCATAG CCAATCAACA TGGCTTGTCT 600
CGCGCCCGGT CGATGCGTTC CTGCATCGCC TGCATCACTT CTTCGTGGGT GTACGATCTG GCGTTGGCGT CGGTGGCTTG CCGCAGGGCT TCCTCAACCT 700
CGCGCGTCAT CCGCTCGTAA TCCTCCGGCC ACAGCGCTTG CTCCATGCGC AGCGACGCCT GACCCAGCAG GTGCAGCAGG CTGAACAGCC GCATGTCGTT 800
GGTGGCGACG ATGCGCTCCC GAATCTGCTC CTGAATAGCT TCGCTAGCCC GATGCGCTTG TGGTGTGGCA GTCCCCTCGT AGTCAGCGGG GAATTCCGCT 900
TCCTCGGCAA TCCTTGCCCA GGCGCTGGCC AGCGCCTCGA TGGTTGACGT GCTCATTTCC GCCGCTCCTG TGCTCTTTGG CGAATCAACC GGCCAAGGGG 1000
CCTCGACGCG AAAACCAGCA GCATCACGCC TATCGGATAC CAGGTCGAGA AGACCACCAA GGCATCGAAG GCTGGCCGTC CGGTGTTGGT AGGCAGTACG 1100
GCGGTCACAG CCATCAACCC GCCGACCGTC CAGATGATGC GCCACACGTA GGGCATCACG CGGGGCACGT AGCCGTCATC GAAAGCGGTC TGGTAGTAGC 1200
GGCGCAGGCC GCGCTCGGCA AGCGCCTGGC GCTGCCGCTC CGACAGCGCA TCCGTCCGGC CATACCAGCG CCGGAATGGT GGACAGCGCA GCAGCAAAGG 1300
GGTGAAGAGG ATCATCACCG CCACGATCGC GACACCCCAC GCCATGACGG CGCCGGCATC GGGCCGCTTG GCGTTCTCGA TCACGGGCGC GAAGACGCCC 1400
GGAGCACCGA TCATCCAGAA CAGCGCAATG TGCTGATGGA AGGAGAGCTT CATTTGCTCA TCCACTTGCG CAGCAGGCGC TTTTTCAGGG AGGCGCGTTC 1500
TTGCGGGTTG CGTCCCTTGT GATTGGCGAT GCGATCCGCC GTGGCGGCCT GGCGCTTGAC GGGCCAGTAG GAGCGGCCAT CCTTGCCCAT CGACCAGACG 1600
CTGCTGGCCT GGTTTTCCAG CAGGGGCAGA TGGGCGCTCA GGGCTTCGGG CGACGCGCTG GTCAGCGCCG TGCGCTCACG GGTGCGCCAG CGCTGATGCC 1700
AGAGCTTCTT GTCCTCGCGC TCGCTGCCGC AGGTCGTGTG CCCGACGATG GGTGTTTTGC GGCGGCTGCG GCTCATAACG TAGTGGTCTC CAAGAACATT 1800
CAGGACTGAT CCCAGGCGTC GATGGTCAAG ACCTGATCGG CAGGACAGGC GGCTACGCGG TCGGGAACTG GATGGTGTTC AGTCTCATGC CGACCCCATT 1900
CGATTGATCG CGTCGCTTGC GGCACGCTGC GACGCAAGGA GGTTTGCGAC CTGGTTTAGC AGCCGTGTCC GCTGCATGTC TGTTACCTAT CTCCCCGACC 2000
GCTCATTGGA CCAACTCCAG AGATTGGGCT CTATCGCTCA GCCAATACGA AACCGGCATT GGCTGATGCC ACAACCGAGA CGAACACAAA TGGCTCGGTA 2100
CCTGTATTTC GCGCCCCGTG CACTTGGCCC GGTCTTGCCA CGGCTATCTC ACCTTCCCTG AGGGCACGAA CAATCCCATT GCCCTGAAAG TAATCAGCCA 2200
TTCCCGACAA AACAGTCCAC GTGTCTTGGC CGTGAGGATG AATGTGAGCC GCAATTTCCT GCCCGGGATG GACATGCCAA ACCACGATAA TTGAGTCTCG 2300
GGTTTCAAGC ACAACGGAAC GAATAGGCTC GCCTTCGGAC GGCTGAACAT ACTCGGCTAC AGAAAATATT CTCGATTCAA CAGTCATCGG AGATCCCTCT 2400
TTCACAGTGC CCCCAGACAG GCTGGATATG AGTTCTAACT CGAATTTGAA CGGGAGGCTG GTCGTGCGGT CGTGCAGTTG CCGATGAGCG TGTCGGCTGC 2500
TGCCTCCTTG CCCACCAGCG AACTCACGTC CGTTGCGGCC ATCCAGAAGG TCTTGCCCGA GCGCGGCTCA TAGGTCGCGG GATCGAACAC GCCAGAGGGG 2600
CCGAACATCG GCGGCTTGAT TGGTCATGGC TCCATGCCTT TGTCGTTACG GTCTTCATCG TCGGCATCCT GACGCACGGC GGGCAATTGC TGGAGCAACG 2700
CCGGCAGCCA TTCCTGTACC GTCTCCCACA CCACATCGAG GTTGATGTCG AAATAGCCGT GAGCCATGCG ATTACGCATA TTGCGCATGC TGCGCCACGG 2800
CACGTCGGCA TGCGCCTGGG TGAACTCGAC GTAGCCATCC ATCACCTTTG TGGCCGCCTC GCCGATGACG ATCAGGCTCA TGATGACGGC CTGCTGGGTG 2900
CGCTTGTCGG CCAAGAAGTC GTCCTTGGCC ATCCCTTCCA CGAAGCTGCG CGCATCGGTT GCGGCCTGCT GAATGTGGTC GAGGTAATCG GGCAGGCGGT 3000
TCTCGCTCAT ATCGGTTGCG CCTCCGCGAG CACCTTGGCC CGGAACTTCG GCGGCAGGTC GCCGGGAGTC AGCAGATCGA CGTCAACGCC GAGCAGCGAT 3100
TTCAGTTCTT CTTCCAAATC GCCCAAGTCC AACAACGTGG CACCGGGCAG CGCATCGACC AACAGGTCGA GGTCGCTGCC ATCCCGGTCG GTGCCATGCA 3200
GCACCGAGCC GAAGACGCGC GGGTTCGCGG CGCGAAAGCG GCCTACCGCT TCACGCACTG CGCTTCGCTT CATGTCAAGC ACAACAGACG GTCGCATGGG 3300
CATCCTTTCT TATCGAAACT CGTTGAGATG ATATGCAATC AAGAATAGAA TTTCAAGAAC TATTTTCGAG AATCGCAATG CCTTGATTCC CGTGCCGCGC 3400
CGGTTGCCTT GGCGGGCTTG TCACAAACCT ACGTTTTCAA GAAGAAGGAA ACCGCATGCC CCGCCGTTCG ATCCTCTCCG CCGCCGAGCG CGAAAGCCTG 3500
CTGGCGTTGC CGGACACCAA GGATGAGTTG ATCCGTCACT ACACGTTCAG CGAAAGCGAC CTCTCCATCA TCCGGCAGCG GCGCGGCCCG GCCAATCGGC 3600
TGGGCTTCGC GGTGCAGCTC TGCTACCTGC GCTTTCCCGG CGTCATCCTT GGCGCTGATG AGCCACCGTT CCCGCCATTG CTGAGACTGG TCGCCAACCA 3700
GCTCAAGGTC GGCATCGAAA GCTGGGACGA GTACGGGCAG CGTGAGCAGA CCCGACGCGA GCACCTGGTC GAGCTGCAAA CGGTGTTCGG CTTCCAGCCG 3800
TTCACGATTG GCCACTACCG GCAGGCTGTC CAGTTGCTGA CCGAGCTGGC CATGCAAACC GACAAGGGCA TCGTGCTGGC CAGAGCCTTG ATCGAGCACC 3900
TGCGGCGGCA GTCGGTCATT GTGCCCGCCC TCAACGCCGT CGAGCGGGCG AGCGCCGAAG CGATTACCCG CGCCAACCGG CGTCTCTACG ACGCCTTGGC 4000
TGAGCCGCTG ACGGACGTGC ATCGCCGTCG CCTCGACGAT CTGCTCAAGC GCCGCGACAA CGGCAAGACG ACGTGGCTGG CCTGGCTGCG GCAATCCCCG 4100
GTCAAACCGA ACTCGCGGCA CATGCTGGAA CACATCGAAC GCCTCAAGGC GTGGCAGGCG CTCGACCTGC CCTCCGGCAT CGAGCGGCTG GTTCACCAGA 4200
ACCGGCTGCT CAAGATCGCC CGCGAGGGCG GCCAGATGAC GCCCGCCGAC CTGGCGAAGT TCGAGCCGCA GCGGCGTTAC GCGACCCTGG TGGCGCTCGC 4300
CATCGAGGGC ATGGCCACCG TCACCGACGA AATCATCGAC CTGCATGACC GCATCCTGGG CAAGCTGTTC AATGCCGCCA AGAACAAGCA TCAGCAGCAG 4400
TTCCAGGCAT CCGGCAAGGC GATCAATGCC AAGGTGCGGC TGTTCGGGCG CATCGGCCAG GCGCTGATCG AGGCCAAGCA AGCGGGCCGC GATCCGTTCG 4500
CTGCCATCGA GGCCGTCATG TCCTGGGATG CTTTCGCCGA GAGCGTCACC GAAGCGCAGC GGCTCGCGCA ACCCGAGGAC TTCGATTTCC TGCACCGCAT 4600
CGGCGAGAGC TACGCCACGC TGCGCCGCTA CGCGCCGGAA TTTCTCGACG TGCTCAAGTT GCGGGCCGCG CCCGCCGCCA AGGACGTACT CGACGCCATC 4700
GAGGTGCTGC GCAGCATGAA CAGCGACAAC GCCCGCAAGG TGCCCACCGA CGCGCCGACC GAGTTCATCA AGCCGCGCTG GCAGAAGCTG GTGATGACCG 4800
ACACCGGCAT CGACCGGCGC TACTACGAAC TGTGCGCGCT GTCGGAGCTG AAGAACGCGC TGCGCTCCGG CGACATCTGG GTGCAAGGCT CGCGCCAGTT 4900
CAAGGACTTC GAGGACTACC TGGTGCCGCC CGCGAAATTC GCCAGCCTCA AGCAGGCCAG CGAATTGCCG CTGGCCGTGG CCACCGATTG CGACCAGTAC 5000
CTGCATGACC GGCTGACGCT GCTGGAAACG CAGCTCCCCA CCGTCAACCG CATGGCGCTG GCCAACGAGC TGCCGGACGC CATCATCACG GAGTCGGGCC 5100
TGAAGATCAC GCCGCTCGAT GCGGCGGTGC CCGATACCGC ACAGGCCCTG ATCGACCAGA CGGCGATGAT CCTACCGCAC GTCAAGATCA CCGAATTGCT 5200
GCTGGAGGTA GACGAATGGA CGGGCTTCAC CCGGCACTTC GCCCACCTGA AGTCAGGCGA CCTGGCCAAG GACAAAAACC TGTTGCTGAC CACGATCCTC 5300
GCCGACGCGA TCAACCTGGG CCTGACCAAG ATGGCGGAAT CGTGCCCCGG CACGACCTAC GCCAAGCTGG CCTGGCTGCA AGCCTGGCAC ATCCGCGACG 5400
AAACCTACGG GGCGGCACTG GCCGAGCTGG TCAACGCGCA GTTCCGACAT CCCTTCGCCG AGCATTGGGG CGACGGCACC ACGTCATCGT CGGACGGCCA 5500
GAACTTCCGC ACCGGCAGCA AGGCCGAGAG CACCGGCCAC ATCAATCCGA AATACGGCAG CAGCCCAGGG CGGACGTTCT ACACCCATAT CTCCGACCAG 5600
TACGCGCCGT TCCACACCAA GGTCGTGAAC GTCGGCGTGC GCGACTCGAC CTACGTGCTC GACGGCCTGC TGTATCACGA ATCCGACCTG CGGATCGAGG 5700
AGCACTACAC CGACACGGCA GGGTTCACGG ACCACGTCTT CGCGTTGATG CACCTGCTGG GCTTCCGCTT CGCCCCGCGC ATTCGTGACC TGGGCGACAC 5800
CAAGCTCTAC ATCCCGAAGG GCGATGCCAC CTACGAGGCG TTGAAACCGA TGATCGGCGG CACGCTCAAC ATCAAGCACG TCCGCGCCCA TTGGGATGAA 5900
ATCCTGCGGA TGGCCACCTC GATCAAGCAG GGCACGGCGA CGGCCTCGCT GATGCTCAGG AAGCTTGGCA GCTACCCGCG CCAGAACGGC CTGGCCGTCG 6000
CCCTGCGTGA GCTGGGACGC ATCGAGCGCA CACTGTTCAT CCTGGACTGG CTGCAAAGCG TCGAGCTGCG CCGCCGCGTG CATGCCGGGC TGAACAAAGG 6100
CGAGGCGCGC AACGCGCTGG CCCGCGCCGT GTTCTTCAAC CGCCTGGGGG AAATCCGCGA CCGCAGCTTC GAGCAGCAGC GCTACCGGGC CAGCGGCCTC 6200
AACCTGGTGA CGGCGGCCAT CGTGCTATGG AACACGGTCT ATCTGGAGCG GGCCGCGAAC GCCTTGCGTG GCCACGGTCA AGCCGTCGAT GACGGCCTGT 6300
TGCAGTACCT GTCGCCGCTC GGCTGGGAGC ACATCAACCT GACCGGCGAT TACCTCTGGC GCAGCAGCGC CAAGATCGGC GCGGGCAAGT TCAGGCCGCT 6400
ACGGCCGCTG CAACCGGCTT AGCGTGCTTT ATTTTCCGTT TTCTGAGACG ACCCC

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnpR TnPa38 34-591 Accessory Gene Resolvase -
WP_003100853.1 TnPa38 585-956 Passenger Gene Hypothetical -
WP_003100856.1 TnPa38 953-1453 Passenger Gene Hypothetical -
WP_003100858.1 TnPa38 1450-1776 Passenger Gene Hypothetical -
cupin domain-containing protein TnPa38 2031-2405 Passenger Gene Other -
WP_003100872.1 TnPa38 2624-3010 Passenger Gene Hypothetical -
nucleotydltransferase TnPa38 3007-3297 Passenger Gene Other -
tnpA TnPa38 3456-6422 Transposase   +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpR TnpR TnPa38 558 34-591 -
Class:   Accessory Gene
Sub Class:   Resolvase
Transpoase Chemistry:   Serine
Sequence Family:  Serine Site-Specific Recombinase
Protein Sequence:  
 MLIGYARVST QDQNLELQRE ALSKAGCKKV FEDKVSGTRA DRPGLAKTLE MLREGDTLVV WKLDRLGRSV KQLVDLVGDL HKHGVQFRSL TDSIDTGTPS
GRFFFHVMAS LAEMERELTV ERTRAGLEVA KQLGRKGGRK PKMTDSKIES AKKLLASGVP PKDVAKNLGV SIPTLYRWVP ASTHA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
WP_003100853.1 WP_003100853.1 TnPa38 372 585-956 -
Class:   Passenger Gene
Sub Class:   Hypothetical
Protein Sequence:  
 MSTSTIEALA SAWARIAEEA EFPADYEGTA TPQAHRASEA IQEQIRERIV ATNDMRLFSL LHLLGQASLR MEQALWPEDY ERMTREVEEA LRQATDANAR
SYTHEEVMQA MQERIDRARD KPC

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
WP_003100856.1 WP_003100856.1 TnPa38 501 953-1453 -
Class:   Passenger Gene
Sub Class:   Hypothetical
Protein Sequence:  
 MKLSFHQHIA LFWMIGAPGV FAPVIENAKR PDAGAVMAWG VAIVAVMILF TPLLLRCPPF RRWYGRTDAL SERQRQALAE RGLRRYYQTA FDDGYVPRVM
PYVWRIIWTV GGLMAVTAVL PTNTGRPAFD ALVVFSTWYP IGVMLLVFAS RPLGRLIRQR AQERRK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
WP_003100858.1 WP_003100858.1 TnPa38 327 1450-1776 -
Class:   Passenger Gene
Sub Class:   Hypothetical
Protein Sequence:  
 MSRSRRKTPI VGHTTCGSER EDKKLWHQRW RTRERTALTS ASPEALSAHL PLLENQASSV WSMGKDGRSY WPVKRQAATA DRIANHKGRN PQERASLKKR
LLRKWMSK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
cupin domain-containing protein Cupin domain-containing protein TnPa38 375 2031-2405 -
Class:   Passenger Gene
Sub Class:   Other
Comment:   cupin domain
Protein Sequence:  
 MKEGSPMTVE SRIFSVAEYV QPSEGEPIRS VVLETRDSII VVWHVHPGQE IAAHIHPHGQ DTWTVLSGMA DYFQGNGIVR ALREGEIAVA RPGQVHGARN
TGTEPFVFVS VVASANAGFV LAER

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
WP_003100872.1 WP_003100872.1 TnPa38 387 2624-3010 -
Class:   Passenger Gene
Sub Class:   Hypothetical
Protein Sequence:  
 MSENRLPDYL DHIQQAATDA RSFVEGMAKD DFLADKRTQQ AVIMSLIVIG EAATKVMDGY VEFTQAHADV PWRSMRNMRN RMAHGYFDIN LDVVWETVQE
WLPALLQQLP AVRQDADDED RNDKGMEP

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
nucleotydltransferase Nucleotydltransferase TnPa38 291 3007-3297 -
Class:   Passenger Gene
Sub Class:   Other
Protein Sequence:  
 MRPSVVLDMK RSAVREAVGR FRAANPRVFG SVLHGTDRDG SDLDLLVDAL PGATLLDLGD LEEELKSLLG VDVDLLTPGD LPPKFRAKVL AEAQPI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA TnPa38 2967 3456-6422 +
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
 MPRRSILSAA ERESLLALPD TKDELIRHYT FSESDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGADEP PFPPLLRLVA NQLKVGIESW DEYGQREQTR
REHLVELQTV FGFQPFTIGH YRQAVQLLTE LAMQTDKGIV LARALIEHLR RQSVIVPALN AVERASAEAI TRANRRLYDA LAEPLTDVHR RRLDDLLKRR
DNGKTTWLAW LRQSPVKPNS RHMLEHIERL KAWQALDLPS GIERLVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LAIEGMATVT DEIIDLHDRI
LGKLFNAAKN KHQQQFQASG KAINAKVRLF GRIGQALIEA KQAGRDPFAA IEAVMSWDAF AESVTEAQRL AQPEDFDFLH RIGESYATLR RYAPEFLDVL
KLRAAPAAKD VLDAIEVLRS MNSDNARKVP TDAPTEFIKP RWQKLVMTDT GIDRRYYELC ALSELKNALR SGDIWVQGSR QFKDFEDYLV PPAKFASLKQ
ASELPLAVAT DCDQYLHDRL TLLETQLPTV NRMALANELP DAIITESGLK ITPLDAAVPD TAQALIDQTA MILPHVKITE LLLEVDEWTG FTRHFAHLKS
GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHIRDET YGAALAELVN AQFRHPFAEH WGDGTTSSSD GQNFRTGSKA ESTGHINPKY
GSSPGRTFYT HISDQYAPFH TKVVNVGVRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGD ATYEALKPMI
GGTLNIKHVR AHWDEILRMA TSIKQGTATA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI
RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAANALRGH GQAVDDGLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA

 References     

1.Yang L, Jelsbak L, Marvig RL, Damkiær S, Workman CT, Rau MH, Hansen SK, Folkesson A, Johansen HK, Ciofu O, Høiby N, Sommer MO, Molin S. Evolutionary dynamics of bacteria in a human host environment. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7481-6. doi: 10.1073/pnas.1018249108. Epub 2011 Apr 25. PubMed ID: 21518885