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Terminal Inverted Repeats | |
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Recombination Sites | |
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Internal Transposable Elements | |
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Internal Repeats | |
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| Name: Tn554Cad |
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| Family: Tn554 |
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| Evidence of Transposition: no |
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| Host |
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| Host Organism: | Staphylococcus aureus 85_2082 | Molecular Source: | chromosome SSCmec |
| Place of Origin: | New Zealand | Date of Isolation: | 2001 |
| | Other Geographic Information: | 1985 |
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| Map |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TTAACCATAG TATAATAAAT AATAGTAGTT AAAGTAGTTC AGGAGGAGAT GTTAAAGGGA GATTTTACTT TAAATAAAAA TTAACAAGGT TTATAATTTG 100
TAGTACTTAA AGTGTTTAAA GAGGGGGGCG CGTGTGAGGG TTCAAAGGAT AGAAGTGGAG AATAAGCCGT ATCCATTGTA TTTATTACTA GATAAAGAAT 200
ACCAGCTAAA TGAATGCTTC TAGTAAAAGG AAAATTATCA GTCAGAGTGA GATCAGCAAA AAAATAGCTG TAATGAATGA AGAAATGCAA GGGTTTTGGG 300
CTAATAATAG TTGGGATATA AGAAAATGTC CACATCCTTC TGCCATAGAA TTAAGTAAGA ATCCTGCTTT AAGAAATCGT TGGGTTCGTT TTGAACGTGT 400
TAAAAATCTG TGGTTAAGAA CAGAATTGAA ATATTTTTAT TTTTACCATT TAAACAATGG AATATGGAAT GCAAAAACTG TCTGGATTAG AAAAGGAACA 500
GTAATTAATA AAATGTTGGA TTTTTTAGAT TTAAAGTATC CCAGCATTAC TTCAATTACT GAAGTTCCTA TTGATAAAGC AATGACGGAG TATAGAACAT 600
ATTTGACAAA ACAGGGTGTT AGAATTACCA CCACTAATTA TAAGATTACT GCTAATCAAG AAAAAACACC TGTAAAAGCT AATTCTTACT ATGTTACTAA 700
TCTTAAACAA TTTATTGAGT TTTATGAGGA TTTTTATTTT GATGGAGAGG AGTGGGATAA AGACGTTTGG GATAGACGAA ACTTACCTTT GCCAGATGAT 800
AAGGTTAACC CAACACAATA TGAATATACA ATTAACTTTA AAGGGTTTCG GAATACATAT TTTAAACAGC TTGTAAAAAG ATATTGTAAG TTAAGATTGA 900
ACATGGATAG CTTTTCCTAT GTAAGTGATA TTGCCCAAAA ACTTAAAGAG TTCTTTAATT TTCTGGACAT AAAGTTTAAA CACGTTCAGA GAGTACACCA 1000
ATTAACGAGA GTGGAAATTG AAGCATATTT AAGTGAACTA AACATGATGG GGATAAAACC TAGTACAATA ACTGGGAGGA TCTCTATATT GGAAGGACTA 1100
TTTAGTACCC TTCATAGGCT AGAATGGGAT GATGTTCCTT CCAAATTATT AATTTATCCC GAGGACTATC CGAAAATACC AAGAGCAAAA CCTCGCTTTA 1200
TAGACGAATT CGTTCTAGAG CAATTGAACA GTCATCTTGA TAAATTACCC GAATATATAG CTACGATGAC TATGATTGTT CAAGAATGTG GAATGAGGAT 1300
AAGTGAATTG TGCACCTTGA AAAAAGGCTG TTTATTAGAG GACAAAGATG GAGATTACTT TTTAAAGTAT TATCAATGGA AAATGAAAAA GGAGCATATA 1400
GTTCCAATAT CTAAAGAGGT AGTTTTACTT ATTAAAGTTC GGGAAGATAA AGTTTCAGAG GAATTTCCAG ATAGTGAATA CCTCTTTCCA AGAAAAGATG 1500
GATCGCCATT AAAACAAGAA ACATTTAGAG GCGAATTAAA TAAATTAGCT TATGAGCAAA ATATAGTGGA TAAATTAGGC GAGATTTATA GATTCCATGC 1600
CCATGCCTTT CGCCATTCAG TAGGAACAAG AATGATTAAC AACGGGGTGC CCCAGCATAT TGTGCAGAAA TTTTTGGGAC ATGAAAGCCC AGAAATGACA 1700
AGCAGATACG CTCATATCTT TGATGAAACT CTAAAAAATG AATTTACTAA ATTTCAAGAA AAACTGGTTA CCAATAATGG AGATGTGCTC AATCTAGATG 1800
ATGATAGTGA AGTCGATGAT GTAGAGCTTC AATGGTTCAA GAAAAATATA AATGCACAAG TGCTTCCAAA TGGTTATTGT AGATTGCCAG TAATAGCAGG 1900
TGGTTGTCCA CATGCGAATG CATGCTTAGA TTGCACTCAC TTCTGTACCA GTAAGCAATT CTTACCACAG CACGAAGAAC AGTTAGAGCG TACAGAAGAG 2000
TTATTAACCA TAGCAAAGGA TAAACAATGG CAAAGACAAA TAGAGACTAA TAGCCGTGTT AAAGAGCGTT TAGAACAAAT CATTGGAAGT TTAACGGGGT 2100
AATTATCAAT GAATAAACAA GTTAGAAATA CAACAGAAAT TGTACGTTTG GCGAAGCAGA AATCACAAAA GACAAGAGAA AAAGTAGACA AAGCGATTTC 2200
TAAATTTTCG ATTGAAGGTA AAGCTATTAA TTTTAATTCA ATAGCAAAGG AAGCTAATGT TTCTAAATCA TGGCTTTATA AGGAACACGA TATTAGGCAA 2300
AGAATCGAAT CCCTTCGTGA GCGTCAAATA ACATCAAATG TAGTCTCAAA ACCCAAGAAA AGTTCTCGTT CGGAGGAAAT CCTTATAAAA ACCTTAAAAA 2400
GAAGAGTTAT GGAATTAGAA AAAGAAAATA AAAAATTACA GAACCAAATT CAAAAATTAT ATGGAGATCT GTATAATAAA GAGTAGTTTT TATTATTAAT 2500
CTGTAGACAG ATTGTGAAAG GATGCACTTA AACTAACGGG GCAGTTATGT TTAAGATACT TTAAAATTTG GTTAAAATCT CCGAAAGGAT TGGTTTCTAA 2600
CATTAGAAAC CAATCCTTTT TAATATTAAT TATATATCAT TCAAACAATC ATTTGACTAT TTGTTTATAG TGGGATATAT TATAATCATC AAACAAACAG 2700
TCATTTGAAT AAGGAGGGTA ATTATGACAA AAGATATGTG TGAAGTTACC TATATTCATG AAGATAAGGT AAACAGGGCT AAAAAAGACC TTGCTAAACA 2800
GAATCCTATG GATGTAGCGA AAGTTTTTAA GGCTCTATCA GATGATACAA GAGTTAAAAT TGCTTATGTT TTGTCTTTAG AGGGAGAGTT ATGTGTTTGT 2900
GATGTAGCTA ATATCATTGA ATCTTCAACG GCTACGGCAT CCCATCATTT AAGATTATTG AAAAATTTAG GTATAGCAAA ATACCGTAAA GAAGGAAAAT 3000
TAGTCTATTA TTCACTAGAT GATGAGCATG TTAAACAGCT TGTAGAAAAA GCTTTCTTGC ATCAAAGGGA GGTTGCTAGT ATTGGATAGT TCAACAAAAA 3100
CATTAACAGA AGATAAACAG GTTTACCGTG TGGAGGGTTT CTCGTGTGCG AATTGTGCTG GGAAGTTTGA AAAAAATGTA AAAGAACTAT CAGGGGTGCA 3200
TGATGCTAAA GTCAATTTCG GAGCTTCCAA AATTGATGTC TTTGGCAGTG CAACCGTTGA AGATCTGGAA AAGGCTGGTG CTTTCGAGAA TCTTAAAGTG 3300
GCACCAGAGA AGGCTAGAAG AAGGGTCGAA CCAGTGGTAA CAGAAGATAA AAATGTTTAC CGTGTGGAGG GATTTTCTTG CGCAAACTGT GCTGGGAAGT 3400
TTGAAAAAAA TGTAAAACAA CTAGCTGGAG TTCAGGATGC AAAAGTGAAC TTTGGCGCTT CTAAAATTGA TGTATATGGA AACACATCGG TTGAAGAGCT 3500
TGAAAAAGCA GGTGCTTTCG AGAATCTTAA GGTAATTCCT GAAAAACTGG CGAATCCATC GATACAAGCG GTCAAAGAAG ACACTAAGGC TCCTAAAGAA 3600
GAGAAAATAC CGTTTTATAA AAAACACAGC ACATTGCTGT TTGCCACATT ACTGATTGCT TTTGGTTACC TTTCTCACTT TGTAAATGGA GAAGATAACC 3700
TTGTAACGTC CATGTTATTT GTAAGTTCGA TTGTAATTGG CGGATATTCA CTATTTAAAG TTGGTTTTCA AAATTTGATA CGCTTTGATT TCGACATGAA 3800
AACCCTGATG ACCGTTGCAG TTATTGGAGC TGCCATCATT GGTGAATGGG CAGAGGCATC CATTGTTGTC ATTCTCTTTG CAATCAGTGA AGCACTTGAA 3900
CGTTTTTCTA TGGATAGAGC AAGACAGTCC ATTCGTTCAT TGATGGATAT TGCCCCAAAA GAAGCACTTG TTAGGCGGAA TGGTCAGGAA ATAATGATCC 4000
ATGTGGACGA TATTGCCGTG GGTGATATTA TGATCGTCAA ACCAGGGGAG AAAATTGCCA TGGATGGGAT CATTATAAAT GGTGTGTCGG CTGTCAACCA 4100
GGCTGCTATA ACAGGAGAAT CTGTCCCTGT TGCCAAAACG GTAGATGATG AAGTATTTGC AGGCACGCTT AACGAAGAGG GACTACTTGA AGTAAAAATC 4200
ACCAAATACG TAGAGGATAC AACTATCTCC AAGATTATTC ATCTGGTTGA GGAAGCACAA GGGGAGCGCG CTCCAGCGCA AGCATTCGTA GATAAATTTG 4300
CGAAATATTA TACGCCGATC ATTATGGTTA TTGCGGCGCT CGTTGCAGTC GTTCCACCTT TATTCTTTGG TGGAAGTTGG GATACTTGGG TTTATCAAGG 4400
ATTAGCGGTA CTTGTAGTTG GATGTCCGTG TGCATTAGTT ATTACTACTC CAATCTCGAT TGTCTCGGCA ATTGGAAATG CAGCTAAAAA AGGTGTGTTG 4500
ATTAAAGGCG GTGTCTATCT AGAGGAATTA GGAGCCATTA AGGCAATCGC ATTTGATAAA ACAGGAACAC TGACAAAAGG TGTACCAGTG GTAACAGATT 4600
TTAAAGTGTT AAATGATCAA GTGGAAGAAA AAGAGCTGTT TTCCATTATT ACAGCTTTAG AATATCGATC ACAACATCCA CTTGCTTCAG CAATAATGAA 4700
GAAAGCAGAG CAAGATAATA TTACTTATTC CGATGTTAGA GTGAAGGACT TCACTTCTAT TACAGGTCGG GGCATTCAAG GGAATATAGA TGGAACAACC 4800
TATTACATTG GCAGTCCAAG GCTTTTTAAA GAATTAAATG TTTCCGATTT TAGCCTTGAG TTTGAAAATA AAGTGAAAGT TTTACAAAAC CAAGGGAAAA 4900
CGGCCATGAT TATTGGAACG GACCAAACAA TCCTCGGCGT GATTGCTGTA GCAGATGAGG TCCGCGAAAC AAGTAAAAAT GTGATTCTAA AACTTCATCA 5000
GTTAGGAATC AAGCAAACAA TTATGCTGAC AGGTGATAAT CAAGGTACCG CAGAAGCAAT CGGTGCTCAT GTAGGCGTTT CTGATATTCA GTCCGAATTG 5100
TTGCCACAGG ATAAGTTGGA CTATATTAAA AAAATGAAAG CCGAGCATGG TAATGTAGCT ATGATTGGCG ATGGCGTCAA TGATGCTCCT GCACTTGCTG 5200
CATCCACTGT TGGCATTGCA ATGGGCGGTG CTGGAACAGA TACTGCCATC GAGACAGCTG ATATTGCATT AATGGGAGAT GATTTAAGTA AGCTTCCATT 5300
TGCAGTAAGA CTTAGCAGGA AAACGCTAAA TATCATCAAA GCGAACATCA CGTTTGCCAT CGGAATTAAG ATAATTGCCT TACTATTGGT TATTCCAGGC 5400
TGGCTAACCC TTTGGATTGC GATTCTTTCC GATATGGGAG CCACTATTTT GGTAGCATTA AATAGTTTGC GACTGATGAG AGTGAAGGAT AAATAAGTAC 5500
TATATGTGTG CAATTTACGG AAGGACTTTT CATTTTGAAG AAAAGTCCTA TCCCTAAAAT ATTTGGAGAT GATAAAATGA TCGCGACGAT ACTGACGGCT 5600
ACTGCGGTAT ATGTAGCAAC AGGAATTGAT TATCTCGTCA TATTAATTCT TTTGTTTTCG CAAGTAAAAA AAGGTCAGGT AAAACATATT TGGATAGGAC 5700
AATATATAGG GACTGCAATT GTGATAGGAG CAAGTCTTTT AGTTGCACAG GGGGTTGTAA ATTTAATTCC TCAGCAATGG GTTATCGGAC TACTTGGACT 5800
TTTACCACTT TACCTAGGCG TGAAAATATG GATTAAAGGA GAAGAGGATG AAGATGAAAG TAGCATTTTA TCTTTATTCT CCTCTGGAAA ATTTAATCAG 5900
TTATTTTTGA CGATGATTTT CATCGTATTG GCTTCCAGTG CGGATGACTT TTCCATTTAT ATACCGTACT TCACGACCTT AAGTATGTCT GAAATCTTTA 6000
TTGTCACTAT TGTCTTTCTA ATTATGGTTG GAGTTTTGTG CTATGTCAGT TACCGTCTAG CTTCCTTCGA TTTTATATCG GAAACAATTG AGAAATATGA 6100
ACGTTGGATT GTACCAATTG TATTCATTGG GCTAGGTATT TATATATTGT TTGAAAATGG AACATTCAAT GCTCTGATTT CATTTCTTCT TTGAAGTGGC 6200
GAATCCAAAC CATAAAGTAA AGTATAAAGA GGTTATCCTA TTGATTGGGA TAACCTTTTT ATATTTAACT AATTATCATT TACGTTAAAC ACATGTACTT 6300
AAAGTAAGAA CATTGATTTA AACCATTTTT TTCATAATTT ACTCTTGATA AGATCCG
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tnpB |
Tn554Cad |
210-2102 |
Transposase |
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+ |
| tnpC |
Tn554Cad |
2109-2486 |
Accessory Gene |
Helper |
+ |
| cadC |
Tn554Cad |
2724-3089 |
Passenger Gene |
Heavy Metal Resistance |
+ |
| cadA |
Tn554Cad |
3082-5496 |
Passenger Gene |
Heavy Metal Resistance |
+ |
| cadD |
Tn554Cad |
5535-6194 |
Passenger Gene |
Heavy Metal Resistance |
+ |
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpB |
TnpB |
Tn554Cad |
1893 |
210-2102 |
+ |
| Class: | Transposase |
Protein Sequence:
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MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN
KMLDFLDLKY PSITSITEVP IDKAMTEYRT YLTKQGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFIE FYEDFYFDGE EWDKDVWDRR NLPLPDDKVN
PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NMDSFSYVSD IAQKLKEFFN FLDIKFKHVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST
LHRLEWDDVP SKLLIYPEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC GMRISELCTL KKGCLLEDKD GDYFLKYYQW KMKKEHIVPI
SKEVVLLIKV REDKVSEEFP DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKLGEIY RFHAHAFRHS VGTRMINNGV PQHIVQKFLG HESPEMTSRY
AHIFDETLKN EFTKFQEKLV TNNGDVLNLD DDSEVDDVEL QWFKKNINAQ VLPNGYCRLP VIAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLT
IAKDKQWQRQ IETNSRVKER LEQIIGSLTG
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpC |
TnpC |
Tn554Cad |
378 |
2109-2486 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Helper |
| Sequence Family: | Tn554_family |
| Comment: | target specificity and orientation |
Protein Sequence:
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MNKQVRNTTE IVRLAKQKSQ KTREKVDKAI SKFSIEGKAI NFNSIAKEAN VSKSWLYKEH DIRQRIESLR ERQITSNVVS KPKKSSRSEE ILIKTLKRRV
MELEKENKKL QNQIQKLYGD LYNKE
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| cadC |
CadC |
Tn554Cad |
366 |
2724-3089 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Cadmium |
| Comment: | cadmium efflux system repressor |
Protein Sequence:
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MTKDMCEVTY IHEDKVNRAK KDLAKQNPMD VAKVFKALSD DTRVKIAYVL SLEGELCVCD VANIIESSTA TASHHLRLLK NLGIAKYRKE GKLVYYSLDD
EHVKQLVEKA FLHQREVASI G
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| cadA |
CadA |
Tn554Cad |
2415 |
3082-5496 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Cadmium |
| Comment: | P-type cadmium-efflux ATPase |
Protein Sequence:
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MDSSTKTLTE DKQVYRVEGF SCANCAGKFE KNVKELSGVH DAKVNFGASK IDVFGSATVE DLEKAGAFEN LKVAPEKARR RVEPVVTEDK NVYRVEGFSC
ANCAGKFEKN VKQLAGVQDA KVNFGASKID VYGNTSVEEL EKAGAFENLK VIPEKLANPS IQAVKEDTKA PKEEKIPFYK KHSTLLFATL LIAFGYLSHF
VNGEDNLVTS MLFVSSIVIG GYSLFKVGFQ NLIRFDFDMK TLMTVAVIGA AIIGEWAEAS IVVILFAISE ALERFSMDRA RQSIRSLMDI APKEALVRRN
GQEIMIHVDD IAVGDIMIVK PGEKIAMDGI IINGVSAVNQ AAITGESVPV AKTVDDEVFA GTLNEEGLLE VKITKYVEDT TISKIIHLVE EAQGERAPAQ
AFVDKFAKYY TPIIMVIAAL VAVVPPLFFG GSWDTWVYQG LAVLVVGCPC ALVITTPISI VSAIGNAAKK GVLIKGGVYL EELGAIKAIA FDKTGTLTKG
VPVVTDFKVL NDQVEEKELF SIITALEYRS QHPLASAIMK KAEQDNITYS DVRVKDFTSI TGRGIQGNID GTTYYIGSPR LFKELNVSDF SLEFENKVKV
LQNQGKTAMI IGTDQTILGV IAVADEVRET SKNVILKLHQ LGIKQTIMLT GDNQGTAEAI GAHVGVSDIQ SELLPQDKLD YIKKMKAEHG NVAMIGDGVN
DAPALAASTV GIAMGGAGTD TAIETADIAL MGDDLSKLPF AVRLSRKTLN IIKANITFAI GIKIIALLLV IPGWLTLWIA ILSDMGATIL VALNSLRLMR
VKDK
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| cadD |
CadD |
Tn554Cad |
660 |
5535-6194 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Cadmium |
| Sequence Family: | Cad (Pfam:PF03596) |
| Comment: | cadmium resistance transporter (or sequestration) family protein |
Protein Sequence:
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MKKSPIPKIF GDDKMIATIL TATAVYVATG IDYLVILILL FSQVKKGQVK HIWIGQYIGT AIVIGASLLV AQGVVNLIPQ QWVIGLLGLL PLYLGVKIWI
KGEEDEDESS ILSLFSSGKF NQLFLTMIFI VLASSADDFS IYIPYFTTLS MSEIFIVTIV FLIMVGVLCY VSYRLASFDF ISETIEKYER WIVPIVFIGL
GIYILFENGT FNALISFLL
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| References |
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| 1. | Ito T, Okuma K, Ma XX, Yuzawa H, Hiramatsu K. Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC. Drug Resist Updat. 2003 Feb;6(1):41-52. doi: 10.1016/s1368-7646(03)00003-7. PubMed ID: 12654286
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