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Transposon
Name: Tn554
Family: Tn554
Evidence of Transposition: yes
 Host     

Host Organism:Staphylococcus aureus Molecular Source:genome
Place of Origin:USA Date of Isolation:1979

 Map     



 Sequence     
DNA SequenceLength  6691 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TTAACCATAG TATAATAAAT AATAGTAGTT AAAGTAGTTT AAGAGGAGAT ATTAAAGGGA TATTTTACTT TAAATAAAAA TTAACAAGGT TTATAATTTG 100
TAGTAGTTAA AGTATTTAAA GAGGTGGGAA CATGTGAAGG TTCAAAGGAT AGAAGTGGAG AATAAGCCGT ATCCATTGTA TTTATTACTA GATAAAGAAT 200
ACCAGCTAAT AGAACCAGTA ATGAAATTTA TTAAATACTT AGATAATACT GGTAAGTCTC CTAATACCAT TAAGGCATAC TGCTATCATT TAAAGTTGCT 300
GTACGAGTTC ATGGAACAGA GAGGTGTTAT TCTTAATGAT ATTAACTTTG AGTTGTTAGC AGACTTCGTA GGTTGGTTGA GATATCCTTC AGCATCAAAT 400
GTAATTGATC TTCAGTCAAA AAAAGCCATA AGAGAAGAAA CGACAGTGAA TACAATTTTA AATGTAGTTA TGAGTTTTCT TGATTATTTA AGTAGATTAG 500
GAGAATTTAA ATCAATTGAT GTATTTAAAC AAGCAAAGGG AAGAAATTTC AAAGGATTTT TACATCATGT TAATAAGGGT AGATACCAAA AGAATGTCTT 600
AAAGTTAAGG GTTAAAAAGA AACAGATAAG AACATTGAGA TCAAAGGAAG TTAAGCAAAT TATTGATGCT TGTCATACGA AAAGAGATAA ATTAATTTTA 700
ATGCTTATGT ATGAGGGTGG TTTAAGAATC GGTGAAGTGT TATCGCTTAG GCTTGAAGAT ATTGTCACTT GGGACAATCA AATCCATTTA ACACCTAGAG 800
ATGTTAATGT TAATGAAGCT TATATTAAAT TAAGGAAGGA AAGAACAATA CATGTGAGTA AAGAACTTAT GTCACTTTAT ACAGATTACT TGATATATGA 900
GTATAGTGAG GAATTGGAGC ATGATTATGT TTTTATTTCC TTAAAAGAAG GCTATTTTGG GAAACCACTA AAGTACCAAA GTGTTCTTGA TCTAGTTAGA 1000
AGAATAGTTA AAAGGACTGG AATAGAATTT ACATCACATA TGCTTCGCCA CACTCACGCA ACGCAGCTAA TTAGGGAAGG ATGGGATGTT GCGTTCGTTC 1100
AAAAGAGATT AGGTCACGCA CATGTTCAGA CAACGTTAAA TACCTATGTT CATCTTTCAG ATCAGGATAT GAAAAATGAG TTTAATAAAT ACCTCGAGAG 1200
AAAGGAGCAT AAGAAATGAA TGCTTCTAGT AAAAGGAAAA TTATTAGTCA GAGTGAGATT AGCAAAAAAA TAGCTGTAAT GAATGAAGAA ATGCAGGGGT 1300
TTTGGGCTAA TAATAGTTGG GATATAAGAA AATGTCCACA TCCTTCTGCC ATAGAATTAA GTAAGAATCC TGCTTTAAGG AATCGTTGGG TTCGTTTTGA 1400
ACGTGTTAAA AATCTGTGGT TAAGAACAGA ATTGAAATAT TTTTATTTTT ACCATTTAAA CAATGGAATA TGGAATGCAA AAACTGTCTG GATTAGAAAA 1500
GGAACAGTAA TTAATAAAAT GTTAGATTTC TTAGATTTAA AGTATCCTAG CATTACTTCA ATTACTGAAG TTCCTATTGA AAAAGCAATG ACGGAGTATA 1600
GAACTTATTT GACAAAACGG GGTGTTAGAA TTACCACTAC TAATTATAAG ATTACTGCTA ATCAAGAAAA AACACCTGTA AAAGCTAATT CCTACTATGT 1700
TACTAATCTA AAACAATTTA TGGAGTTTTA TGAGAACTTT TATTTTGATG GAGAGGAGTG GGATAAAGAC GTTTGGGATA GACGTAACTT ACCTTTGCCA 1800
GATGATAAGG TTAACCCAAC ACAATATGAA TATACAATTA ACTTTAAAGG GTTTCGGAAT ACATATTTTA AACAACTTGT AAAAAGATAT TGTAAGTTGA 1900
GATTGAACGT GGATAGCTTT TCCTATGTAA GTGATATTGC CCAAAGACTT AAAGAGTTCT TTAATTTTCT GGACATGAAA TTTAAACAAG TTCAGAGAGT 2000
ACACCAATTA ACGAGAGTGG AAATTGAAGC ATATTTAAGT GAACTAAACA TGATGGGAAT AAAACCTAGT ACAATAACTG GGAGGATCTC TATATTGGAA 2100
GGACTATTTA GTACCCTTCT TAGGCTAGAA TGGGATGATG TTCCTTCCAA AATATTAATT TATTCTGAGG ACTATCCGAA AATACCAAGA GCAAAACCAC 2200
GCTTTATAGA TGAATTCGTC CTAGAGCAAT TGAACAGTCA TCTTGATAAA TTACCCGAAT ATATAGCTAC GATGACTATG ATTGTTCAAG AATGTGGAAT 2300
GAGGATAAGT GAATTGTGCA CCTTGAAAAA AGGCTGTCTA TTAGAGGACA AAGATGGAGA TTTCTTTTTA AAGTATTATC AATGGAAAAT GAAAAAGGAG 2400
CATATAGTTC CAATATCTAA AGAGGTAGCT TTACTTATTA AAGTTCGGGA AGATAAAGTT TCAGAGGAAT TTCCAGATAG TGAATACCTC TTTCCAAGAA 2500
AAGATGGATC GCCATTAAAA CAAGAAACAT TTAGAGGTGA GTTAAATAAA TTAGCTTATG AGCAAAATAT AGTGGATAAA TCAGGTGAGA TTTATAGATT 2600
CCATGCCCAT GCCTTTCGCC ATACAGTAGG AACAAGAATG ATTAACAACG GGATGCCCCA GCATATTGTG CAGAAATTTT TGGGGCATGA AAGCCCAGAA 2700
ATGACAAGCA GATACGCTCA TATCTTTGAT GAAACTCTAA AAAATGAATT TACTAAATTT CAGGAAAAAC TGGTTACCAA TAATGGAGAT GTGCTTGATC 2800
TAGATGAAGA TAATGAAGTC GATGATGTAG AGCTTCAATG GTTCAAGAAA AATATAAATG CACAAGTGCT TCCAAATGGT TATTGTAGAT TGCCAGTAGT 2900
AGCAGGTGGT TGTCCACATG CGAATGCATG CTTAGATTGC ACTCACTTCT GTACCAGTAA GCAATTCTTA CCACAGCACG AAGAACAGTT AGAGCGTACA 3000
GAAGAGTTAT TAGCCATAGC TAAGGATAAA CAATGGCAAA GACAAGTAGA GACTAATAGC CGTGTTAAAG AGCGTTTAGA ACAAATCATT GGAAGTTTGA 3100
CGGGGTAATT ATCAATGGAT AAACAAGTTA GAAATACAAC AGAAATTGTA CGTTTGGCGA AGCAGAAATC AAAAAAGACA AGGGAAAAAG TAGACAAAGC 3200
GATTTCTAAA TTTTCGATTG AAGGTAAAGT TATTAATTTT AATTCAATAG CAAAGGAAGC TAATGTTTCT AAATCATGGC TTTATAAGGA ACACGATATT 3300
AGGCAAAGAA TCGAATCCCT TCGTGAGCGT CAAATAACAG CAAATGTAGT CTCAAAACCC AAGAAAAGTT CTCGTTCGGA GGAAATCCTT ATTAAAACCT 3400
TAAAAAGAAG AGTAATGGAA TTAGAAAAAG AAAATAAAAA ATTACAGAAC CAAATTCAAA AATTATATGG AGATCTGTAT AATAAAGAAT AATTATTAAT 3500
CTGTAGACAA ATTGTGAAAG GATGTACTTA AACGCTAACG GTCAGCTTTA TTGAACAGTA ATTTAAGTAT ATGTCCAATC TAGGGTAAGT AAATTGAGTA 3600
TCAATATAAA CTTTATATGA ACATAATCAA CGAGGTGAAA TCATGAGCAA TTTGATTAAC GGAAAAATAC CAAATCAAGC GATTCAAACA TTAAAAATCG 3700
TAAAAGATTT ATTTGGAAGT TCAATAGTTG GAGTATATCT ATTTGGTTCA GCAGTAAATG GTGGTTTACG CATTAACAGC GATGTAGATG TTCTAGTCGT 3800
CGTGAATCAT AGTTTACCTC AATTAACTCG AAAAAAACTA ACAGAAAGAC TAATGACTAT ATCAGGAAAG ATTGGAAATA CGGATTCTGT TAGACCACTT 3900
GAAGTTACGG TTATAAATAG GAGTGAAGTT GTCCCTTGGC AATATCCTCC AAAAAGAGAA TTTATATACG GTGAGTGGCT CAGGGGTGAA TTTGAGAATG 4000
GACAAATTCA GGAACCAAGC TATGATCCTG ATTTGGCTAT TGTTTTAGCA CAAGCAAGAA AGAATAGTAT TTCTCTATTT GGTCCTGATT CTTCAAGTAT 4100
ACTTGTCTCC GTACCTTTGA CAGATATTCG AAGAGCAATT AAGGATTCTT TGCCAGAACT AATTGAGGGG ATAAAAGGTG ATGAGCGTAA TGTAATTTTA 4200
ACCCTAGCTC GAATGTGGCA AACAGTGACT ACTGGTGAAA TTACCTCGAA AGATGTCGCT GCAGAATGGG CTATACCTCT TTTACCTAAA GAGCATGTAA 4300
CTTTACTGGA TATAGCTAGA AAAGGCTATC GGGGAGAGTG TGATGATAAG TGGGAAGGAC TATATTCAAA GGTGAAAGCA CTCGTTAAGT ATATGAAAAA 4400
TTCTATAGAA ACTTCTCTCA ATTAGGCTAA TTTTATTGCA ATAACAGGTG CTTACTTTTA AAACTACTGA TTTATTGATA AATATTGAAC AATTTTTGGG 4500
AAGAATAAAG CGTCCTCTTG TGAAATTAGA GAACGCTTTA TTACTTTAAT TTAGTGAAAC AATTTGTAAC TATTGAAAAT AGAAAGAAAT TGTTCCTTCG 4600
ATAGTTTATT AATATTAGTG ACATTTGCAT GCTTCAAAGC CTGTCGGAAT TGGTTTTTAG TGAAAAGAAC ACGATATTCA CGGTTTACCC ACTTATAAAC 4700
AAAAGATCGA TACTTTTTGT AGTCCTTCTT TGAAATCAAT GGTTGATGTC GTTCAAGAAC AATCAATACA GAGTCTACAC TTGGCTTAGG ATGAAAATAT 4800
AGTGGTGGTA CTTTTTTGAG CATTTTTATA TCCATCTCCA CCATTAATAG TAAACCCAAA GCTCGTTGCA GATTTTGCAA TCTTTTCGCA AATCCCTTCT 4900
CAACGATAAG ATAGCTATAT TTAGCCTGAC TTTCAAAGGT AATTCTTTTG ACAATATCCG TACTGATGTT ATAAGGAATA TTACCATATA TCTTATAGTT 5000
TATATGTTTT GGGAAGGAAA ATTTTAGAAT ATCCGTTTGA ATCACTTTTA TATTCTCAGA GGGGTTTACC GCTTCTTTAG TCACTTGACA TAAGCCTCCA 5100
TCAATTTCTA TAGCAGTAAC TGATCGACTC ATTTTGACTA GCTCTTTGGT AAAATGTCCT TTTCCTGATC CGATTTCTAT TACGTTGTCT TGTTTACTGA 5200
TATTCGTGTG ATTCAATATT TCTTTTACAT GCTTTTTAGA AGTAATAAAA TTTTGCGTGT CTTTAGGGTT TTTCTGGTTC ATTATAACCT TCTCCTTACT 5300
GGTTATAATG AACTGACATT AAGCGTTCAT TATAACCAAT TAATTTTGAT TTGGTTGATA ACGAACTTTA TTAATAACAA ATATAGAAAA AGTACCCATA 5400
CTTTTATCTC CTTTCCATTT TTTTCATTAT GAATGAGATA AAGTAATTTC TACTACTGCG ATACTGGTGC ACATAATTGA AACCTCCTTA TGTAACTGAA 5500
TATGATTGTA TCATCTTTTC ATTTTAGAAT AAACCTTTAT ATGTAATTTT GCACCTTGAC GACTTCAGAT GGACGTTTTC TTCAAAATAA GTATGTTTTG 5600
AAACTTGATA AGAGAAGCCA CTGTCTTTAC TAAAAAGTAA AGGCTGCGAT AAAGGACAGT GACTTTAATA AAGTTGCACT GTTGTATTGT CTTTGGATAA 5700
AATTGTCTAA AGGGAAGTTT TATCCCCAAC GAAAAAACAG TCTAAAGTGG TAAGTAATGA AAGAATGTCG ATAAAGTAGG AATGGGGGAT CTTTTTGAAT 5800
ACAATAGAAT CACAGTTTGA TAAGGTTGCA GAAGATTACG ATTTCGTGAA TGAGCTTTTG AATGATTATT CATTCTTTGT GTCTAATATG TCTCCAAAGA 5900
AAGGCAGAGC ATTAGATATC GGATGTGGCT CGGGTTTGTT AGTGGAGAAA TTAGCAAGTT ATTATGATGA AGTGGTAGGG ATTGATATTT CTAATCAAAT 6000
GCTCGATCTT GCCAAATCTA AACGTCAACT AACAAATACG GTCTATCTGA ATATGAATGC AGAACAACTT AATTTTAATG AGAAGTTTGA TTTTATTGTA 6100
AGCCGAACAA CCTTTCATCA TTTGGATGAT ATAGCCAGCG TAATACAGCA AATGAAGGAA CTGTTGAATG AGGAAGGAAG AATAGTTATT CTTGATAATG 6200
TATCTGAAGT CGAGACACCG CCTACCTATG TTTATAAATT AGGGGCAATC CAAGAGTTTT TACCGCATTG TTTCAAATTT GGAATAAAAA ATGCGATTAG 6300
AATTTATAAT CACAACACAT CTAAATCATG GCTTGAGCAT TTAGCTTCAG ATAAGTACCT ATCTGAACAA AATTATTACG ATTTATATGA AAAGTTGTTG 6400
CCTGGATGCC AATTCCATAA AATGGGTTGG GCAATGGGGG TTGTCTGGAC AAAATAACTT AAAGCTGTGG CTTTGAAAAG TTGAATTTGT GATTGTTTTC 6500
AGAGCTATCG GGTGCTTTAG TATAAATAAC AATGAATAAA AAATCAGCCT GTATCTTATG TTACTATTAA GTAATTTAAG ATACAGGTTT TACTATTTTT 6600
GAAATAAGTA CCTTCCACTT CAAATACATG TATTCAAAGT GAAAGTATTG ATTTAAATGA TTCTTTTGTA ATTTACTCTT GATAAGATGT A

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnpA Tn554 134-1219 Transposase   +
tnpB Tn554 1216-3108 Transposase   +
tnpC Tn554 3115-3492 Accessory Gene Helper +
ANT(9)-Ia (ARO:3002630) Tn554 3643-4425 Passenger Gene Antibiotic Resistance +
ErmA (ARO:3000347) Tn554 4551-5282 Passenger Gene Antibiotic Resistance -
SAM-dependent methyltransferase Tn554 5795-6457 Passenger Gene Other +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA Tn554 1086 134-1219 +
Class:   Transposase
Protein Sequence:  
 MKVQRIEVEN KPYPLYLLLD KEYQLIEPVM KFIKYLDNTG KSPNTIKAYC YHLKLLYEFM EQRGVILNDI NFELLADFVG WLRYPSASNV IDLQSKKAIR
EETTVNTILN VVMSFLDYLS RLGEFKSIDV FKQAKGRNFK GFLHHVNKGR YQKNVLKLRV KKKQIRTLRS KEVKQIIDAC HTKRDKLILM LMYEGGLRIG
EVLSLRLEDI VTWDNQIHLT PRDVNVNEAY IKLRKERTIH VSKELMSLYT DYLIYEYSEE LEHDYVFISL KEGYFGKPLK YQSVLDLVRR IVKRTGIEFT
SHMLRHTHAT QLIREGWDVA FVQKRLGHAH VQTTLNTYVH LSDQDMKNEF NKYLERKEHK K

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpB TnpB Tn554 1893 1216-3108 +
Class:   Transposase
Protein Sequence:  
 MNASSKRKII SQSEISKKIA VMNEEMQGFW ANNSWDIRKC PHPSAIELSK NPALRNRWVR FERVKNLWLR TELKYFYFYH LNNGIWNAKT VWIRKGTVIN
KMLDFLDLKY PSITSITEVP IEKAMTEYRT YLTKRGVRIT TTNYKITANQ EKTPVKANSY YVTNLKQFME FYENFYFDGE EWDKDVWDRR NLPLPDDKVN
PTQYEYTINF KGFRNTYFKQ LVKRYCKLRL NVDSFSYVSD IAQRLKEFFN FLDMKFKQVQ RVHQLTRVEI EAYLSELNMM GIKPSTITGR ISILEGLFST
LLRLEWDDVP SKILIYSEDY PKIPRAKPRF IDEFVLEQLN SHLDKLPEYI ATMTMIVQEC GMRISELCTL KKGCLLEDKD GDFFLKYYQW KMKKEHIVPI
SKEVALLIKV REDKVSEEFP DSEYLFPRKD GSPLKQETFR GELNKLAYEQ NIVDKSGEIY RFHAHAFRHT VGTRMINNGM PQHIVQKFLG HESPEMTSRY
AHIFDETLKN EFTKFQEKLV TNNGDVLDLD EDNEVDDVEL QWFKKNINAQ VLPNGYCRLP VVAGGCPHAN ACLDCTHFCT SKQFLPQHEE QLERTEELLA
IAKDKQWQRQ VETNSRVKER LEQIIGSLTG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpC TnpC Tn554 378 3115-3492 +
Class:   Accessory Gene
Sub Class:   Helper
Sequence Family:  Tn554_family
Comment:   target specificity and orientation
Protein Sequence:  
 MDKQVRNTTE IVRLAKQKSK KTREKVDKAI SKFSIEGKVI NFNSIAKEAN VSKSWLYKEH DIRQRIESLR ERQITANVVS KPKKSSRSEE ILIKTLKRRV
MELEKENKKL QNQIQKLYGD LYNKE

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
ANT(9)-Ia (ARO:3002630) ANT(9)-Ia Tn554 783 3643-4425 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  ANT(9) (ARO:3000228)
Protein Sequence:  
 MSNLINGKIP NQAIQTLKIV KDLFGSSIVG VYLFGSAVNG GLRINSDVDV LVVVNHSLPQ LTRKKLTERL MTISGKIGNT DSVRPLEVTV INRSEVVPWQ
YPPKREFIYG EWLRGEFENG QIQEPSYDPD LAIVLAQARK NSISLFGPDS SSILVSVPLT DIRRAIKDSL PELIEGIKGD ERNVILTLAR MWQTVTTGEI
TSKDVAAEWA IPLLPKEHVT LLDIARKGYR GECDDKWEGL YSKVKALVKY MKNSIETSLN

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
ErmA (ARO:3000347) ErmA Tn554 732 4551-5282 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic target alteration (ARO:0001001)
Target:   streptogramin B antibiotic (ARO:0000050)||lincosamide antibiotic (ARO:0000017)||streptogramin A antibiotic (ARO:0000034)||macrolide antibiotic (ARO:0000000)||streptogramin antibiotic (ARO:0000026)
Sequence Family:  Erm 23S ribosomal RNA methyltransferase (ARO:3000560)
Comment:   perfect match to reference sequence for ARO:3000347||Synonyms: ermTR
Protein Sequence:  
 MNQKNPKDTQ NFITSKKHVK EILNHTNISK QDNVIEIGSG KGHFTKELVK MSRSVTAIEI DGGLCQVTKE AVNPSENIKV IQTDILKFSF PKHINYKIYG
NIPYNISTDI VKRITFESQA KYSYLIVEKG FAKRLQNLQR ALGLLLMVEM DIKMLKKVPP LYFHPKPSVD SVLIVLERHQ PLISKKDYKK YRSFVYKWVN
REYRVLFTKN QFRQALKHAN VTNINKLSKE QFLSIFNSYK LFH

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
SAM-dependent methyltransferase SAM-dependent methyltransferase Tn554 663 5795-6457 +
Class:   Passenger Gene
Sub Class:   Other
Comment:   perfect match to reference sequence for Uniprot A0A347WKB4 and Q5HNP8||Synonyms:
Protein Sequence:  
 MNTIESQFDK VAEDYDFVNE LLNDYSFFVS NMSPKKGRAL DIGCGSGLLV EKLASYYDEV VGIDISNQML DLAKSKRQLT NTVYLNMNAE QLNFNEKFDF
IVSRTTFHHL DDIASVIQQM KELLNEEGRI VILDNVSEVE TPPTYVYKLG AIQEFLPHCF KFGIKNAIRI YNHNTSKSWL EHLASDKYLS EQNYYDLYEK
LLPGCQFHKM GWAMGVVWTK

 References     

Murphy E, Huwyler L, de Freire Bastos Mdo C. Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants. EMBO J. 1985 Dec 1;4(12):3357-65. doi: 10.1002/j.1460-2075.1985.tb04089.x. PubMed ID: 3004956