Transposon
Name: Tn5501.10
Family: Tn3        Group: Tn3000
Evidence of Transposition: no
 Host     

Host Organism:Pseudomonas aeruginosa PA1280 Molecular Source:plasmid pICP-4GES
Place of Origin:Wenzhou, China Date of Isolation:2017
Other Geographic Information:96-year-old male patient with an upper respiratory tract infection at the host

 Map     



 Terminal Inverted Repeats (IR)     

IRL (Length: 38 bp)GGGGTTCTAAGCCGGAACCGCCGAAAATTCCGTCAGCC
IRR (Length: 38 bp)GGGGTTCTAAGCCAGAACCGCCGAAATTTCCGTCATCC

 Sequence     
DNA SequenceLength  6786 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGGGTTCTAA GCCGGAACCG CCGAAAATTC CGTCAGCCGA TCAACGTGGC TTGTCCCGCG CCCGGTCGAT GGGGTAGACC CAACAGTCGT GTCACTAGCC 100
GCCATTTCGA TCACGGCAAT GCCAGCCGGA CGTCACGTCC AGATTGTTCC GGTCTGGATG AGGCCGACTG ACGTCTCGGA TGACGGGTGG CATACAACTG 200
CTGTGAGTCC TGCAGGGGGG CAGCTGCCTG ACCGGACGGC GAGCATCAGC CCATCTCATG TATTAGTCAT GTCAGCTTTG ACACTGCGCA CGCGACGGCA 300
CCCGACCCAT AGTGGTCATT CCACTGCTCA ACGGTAAGAG CAGCCGCATC CTTCGATGCC TCCACATGCC GAGAGCTAAT TGTCTCGGCA CTCTTGCTCC 400
AGCCAGCCAC GGAAAGCTTT CATCCCGTCG GTCTCTTTGC GTGGCTTGAG CTTGGTCAAC CAATAGCTGC CCGTCTCAAT CCGAGTGTCA AATAAGGTCA 500
CCAGCCGACC GCCGAGTAGG TCGCGACCAA AGAGACGAAT CGGCAACAAA GCCACACCTG TTCCTTGTGC GGCTGCATCA GCAAGTACAA GGGATGAGTC 600
AAACACCGGA CCCGTCAAAT GTGGGGGGTT TAACCCAACC GCCTGAAACC ATGCAGTCCA CTCGTCTGCT CGATAAGACC GCAATAGCGT GACCCGTGCC 700
AAATCACTGG GGGTGTGGAT TCGACTTGCC ATGAAAGGGG CGCAAACAGG TGCGAGTGGC GCAGAAAAAA GCTTCGACGC TTCTGTGCCG TGCCAGTGGC 800
CGTCCCCAAA GCGGATTGCG AAGTCCAGGC CATCGCCTGC CAAGTCGACT CGGTTGTTGT TGGAAAACAG TCGAAGATCT ATGAAGGGGT GCTTGACTTG 900
AAAGTCGCGG AGGCGTGGCA GCAACCAGCC CACTGCAAAC GTGCCGACGA CGCCCACAGA CAAAACTTCT TTCTTATGAG GCGTCTTGAC CTTCTCAAGC 1000
GTTAAACCGA TCCGTTCAAA AGCATCGGTC AACACTGGTA GCAGGGCAGC ACCTTCATCA GTCAAAGCAA GCCCTCGCGG GATACGCCGA AAGAGTTTCT 1100
TGCCCAGGCG GTCTTCTAAG TTCTTGACGT GCTGACTGAC GGCGGTCTGC GTCAGGTGCA GTTCAAGGCC CGCCCGGGTG AAGCTCAGAT GGCGTGCAGA 1200
CACCTCAAAT GCGCGCAGCG CGTTAAGCGG AAGGTCCATG ATGATCTAAG TTTTTCTTGG GCCTGGGCTG CAGATTTTAC GTTTGAAGAG ACCACCGACG 1300
ATCCATATGA TCACTCATTT TCAAACAGTA GGAACCATGA AAAGACGCAA CTTCTCCGTC GGGATGGCAT TTTCTGCCTT TGGCTTCAGT GCGATTTTCC 1400
CCAAGAAAAG CTTTGCCTTG GGCGAGCAAG GACAGATGAC TGCTGTGTTT CAGCAACTCG AGGCTAAAGC CCAAGGCCGG TTGGGCGTAC ATGTCATAGA 1500
CACAGCGACT GGTCACGAAT TTGGCTACCG CTCTGACGAA CTTTTCATGA TGCTCAGTTC GTTCAAGCTG CTGGCGAGTG CCTTGGTGCT GGCGCGCGCG 1600
GATCGTGGTG AAGAGTCGCT CACTCGGCGT ATTCGTTACC GCAAGCAGGA CTTGGTGCCT TGGTCGCCAG TGACAGAGGC ATATGCGGAT GGCGAGGGGT 1700
TGACTTTGGC CCAACTCTGT CACGCGACGA TAACCACCAG CGACAACACA GCGGGCAATC TCATACTAGC CAGCTACGGT GGCCCTCAAG CTCTCACCCA 1800
GTATGCCCGC CAACTCGGTG ACAAGATCAC GAGGCTCGAT CGGAACGAAC CCGACCTCAA CACCCGAGTC GAAGGAGGAA GCTTGGATAC GACCTCACCG 1900
CGAGCCATGG CCATGACCAT GAACAAGCTC CTCTTGGGCG ATGCACTCTC ACCCTTATCG CGGAATTTGC TCCGCCAGTG GCTGCTCGAA AACACGACCG 2000
GGGGAAAGCG CCTCAAGGCC GGAACCCCTG CTGATTGGAC CGTTGGCGAC AAGACCGGGA CCAACAAGAC AGATGCGAAC GACATAGGAA TATTGCTACC 2100
ACCGCAAGGC GCACCGGTCC TTGTGACTGC TTACCTCGCT GACAGCACGG CAAGCAGCCA AATAAAAGAT GCAACGCTTG CAGAGGTAGG TAGGCTCACC 2200
TCTATAGGCA TACGATGAAG TCGGAGCGGG GATAAGGACT CGTTCCAGCA ACCGCTCCTG GCCGCCTGCC GCCGACCGCG GTCTGGCTCG AAAGCAGTCT 2300
GTCAACGCGC CGTTTCATTG TTGCCATGAT CATATCTTAA TCGCGCACAG GCGGCCACAG GCGGGCAGTA TGAACCAGCG TCAGTATCCA CACCGTTTCG 2400
CCGTCGATCT GATACACCAG GCGATAGCTT TCGTGCGGGA TCAACTCGCG GGTCCCGGGA ATCTTTCCCG GCTTGCCCAG CATGGGGTGC TGGATCAAGC 2500
GGGCGGCCGC GTCGCTGAAA ATCTCATCCA TCCGGGCCGC CGCGCGCGGA TTGTCGGCTG CGATGTAGTC CCACACATCG GCACGGTCTT GCTGCGCTTC 2600
GGGCGTCCAA ACAACCCTCA CGCCTGGCTC GCCACACTGG CACGCCGTGC GGCGAATTCG GCCTCAACTT CATCGTTCGA CCGCCCCAAT CCAGCGCGCA 2700
TCGAAGCCCG GCCGGCTTCG ACCTTGCGGC GCAGGAACTC GTCGTACTCG CGCGACTCGC GCTGGCGCTG AACGAACTCG CGCATCAGCT CGCGCAGCAC 2800
TTGCGACGCC GGGCGATGGG CCGCCTCGGC TTCGGCCATA AACTCGGCGC GCAACTCAGG CTCCAGCTTC ATCGTGAAAA CGGCTTGTTT TGACATGATC 2900
GGGGCCTCCT GCCACTTGAT ACTAACAGAG TATATACGCC GTCATTACTA AGCGCTATTC ACAGAACGCT GCAAGGCGGG CGTGCGCTAG GCCAAGGCCT 3000
GTCGGAAAAC ATTTGTTTTT CGACAGGCCT TCAACGGTCC TCTGCACCAA CCTCCGAGTG GCCGCAAAAT TGTGCGGAAA ACTCTGTCGC CAGACGCTAC 3100
CATACGGAAA CCTCGTCTTA ATGGTTTTCC GCTTATGTTG GTAGGTTACA TGCGCGTGTC GTCGGACTCC GACCGCCAGA GCACGAACTT GCAGCGCGAT 3200
GCGCTGCTCG CCGTCGGCGT CGATGCGCGG CATCTGTTCG AGGATCATGC TTCCGGCGCG AAGGACGACC GCGCGGGCCT GGCGCGGGCG CTCGAATTCG 3300
TTCGCCCTGG CGACGTGTTG GTCGTGTGGA AGCTCGACCG GCTCGGCCGT TCGTTGTCGC ACTTGCTCGC CATCGTGACC TCGCTCAAGA AAAAGCAGGT 3400
GGCGTTCCGC TCGCTGACGG AGAACCTGGA TACCACGACG CCCTCGGGCG AGTTTCTGTT CCAGGTGTTC GGCGCGCTCG CGCAGTACGA ACGCGCCTTG 3500
ATCCAGGAAC GTGTCGTCGC CGGTCTGGCT GCCGCCCGCA AACGCGGCCG GATCGGCGGC CGGCCGCAGG CGATCACCGG CGAGAAGCTG GAGGCCATCG 3600
TCGCTGCGCT CGATGGCGGC ATGTCCAAGG CGGCGGTGTG CCGCAACTTC GGCGTCAAGC GAACCACGCT GATCGAGACC CTGGCACGGG TTGGTTGGAC 3700
GGGCTCTCGT GGAGCGTCAT CGCGATGACG ACCAAGAGCG AACGATTGAC CGTCCTGTCG GACGCCGAGC AGGAAGCCCT GTACGGCCTG CCGGACTTCG 3800
ACGACGCCCA GCGGCTGGAA TACTTGGCGT TGACTGAAAC CGAACTGGCG CTCGCCAGCA GCCGGCCTGG TCTCCATGCC CAGGTCTATT GCATCTTGCA 3900
GATCGGTTAC TTCAAGGCCA AGCATGCCTT CTTCCGCTTC GACTGGAGTG AGGTCGAGCA CGATTGCGCC TTCGTGCTGA GCCGCTACTT CCACGGCGAG 4000
TCCTTCGAGC ACAAGCCAAT CTCCAAGCAC GAGCACTACA CCCAGCGCGA GTGGATTGCC GATCTGTTCG GCTACCGGCC GTGGGCGGCC GAGTTCCTGG 4100
CGCAGCTCGC GCAGCAGGCC GCGCAGACCG TGCGGCGCGA CGTGATGCCG GGGTTCATCG CCGCCGAGCT GATCGTCTGG CTAAACGAGC ACAAGATCAT 4200
CCGGCCCGGC TATACCACCC TGCAAGAGCT GGTGAGCGAA GCCCTGTCCG CCGAGCGTCG GCGGCTGGCT GGCCTGCTGT CGGAAGTGTT GGACGAATCG 4300
GCCAAGGCCG CGCTGGGTCG GCTTCTAGTG CGTGACGACA CCCTGTCGCA ATTGGCGGCG CTCAAGCAGG ACGCCAAGGA CTTTGGCTGG CGTCAGATGG 4400
CCCGCGAACG CGAAAAGCGC GCCACGCTGG AGCCGCTGCA CCGGATCGCC AAGGCGCTGC TGCCCAAGCT CGGCGTCTCG CAGCAGAATC TGCTGTACTA 4500
CGCCAGCCTG GCGAACTTCT ACACCGTCCA CGATCTACGC AACCTGAAGG CCGATCAGAC CTACCTCTAC CTGCTTTGCT ATGCCTGGGT GCGCTACCGG 4600
CAGCTTTCCG ACAACCTGGT CGATGCGATG GCCTACCACA TGAAGCAGTT GGAGGACGAA AGCAGTGCGG GCGCAAAGCA ATCCTTTGTC GCCGAGCAGG 4700
TGCGCCGTCA GCAAGACACA CCGCAGGTCG GCCGCCTGCT GTCGCTTTAC ATCGACGACA GCGTGCCCGA TCCCACGCCG TTCGGCGATG TGCGCCAGCG 4800
CGCCTACAAA ATCATGCCCC GCGATACGCT GCAAACCACC GCGCAGCGCA TGAGCGTGAA GCCGGTGAGC AAGCTGGCTT TGCACTGGCA GGCGGTGGAC 4900
GGCCTGGCTG AGCGCATCCG CCGCCATCTT CGGCCGCTGT ATGTCGCGCT CGACCTCGCT GGCACTGATC CGGGCAGCCC GTGGCTCGTG GCGCTGGCCT 5000
GGGCCAAGGA CGTGTTCGCC AAACAGCAGC GCCTATCGCA ACGGCCGCTC GCCGAATGTC CAGCGGCCAC GCTGCCGAAA CGCTTGCGAC CGTACCTGCT 5100
GACCTTCGAT GCCGATGGCA AGCCGACGGA CCTGCATGCC GACCGCTACG AGTTCTGGCT GTACCGCCAG GTCAGGAAGC GCTTCCAGTC GGGTGAACTC 5200
TACCTCGACG ACAGCTTGCA GCACCGGCAT TTTTCCGACG AGCTGGTTTC GCTGGATGAG AAGGCCGCCG TGCTGGCGCA GATCGACATC CCGTTCCTGC 5300
GGCAGCCACT CGATGCCCAG CTCGATGCGC TCGCGACCGA GCTGCGCGCT CAGTGGCTGG CCTTCAACCG CGAGCTGAAG CAGGGCAAGC TGACGCACCT 5400
AGAATACGAC AAGGACACGC AGAAGCTGAC ATGGCGCAAG CCCAAGGGCG AGAACCAGAA GGCGCGCGAG AAGGCGTTCT ACGAGCAACT GCCGTTCTGC 5500
GACGTGGCCG ACGTGTTCCG CTTCGTCAAC GGCCAGTGCC AGTTCCTGTC GGCGCTGACG CCTTTGCAGC CGCGCTATGC GAAGAAGGTC GCCGACGCCG 5600
ACAGCCTGAT GGCGGTCATC ATCGCGCAGA CGATGAACCA CGGCAACCAG GTCATGGCAC GCACCAGCGA CATCCCGTAC CACGTGCTGG AGAGCGCCTA 5700
CCAACAGTAC CTGCGCCACG CAACGCTGCA CGCGGCCAAC GACTGCATCA GCAACGCCAT CGCCGCGCTG CCGATCTTCC CGTACTACTC GTTCGACCTC 5800
GATGCACTGT ACGGTGCCGT CGATGGTCAG AAATTCGGCG TCGAGCGGCC GACCGTGAAA GCGCGCCACT CGCGCAAATA CTTTGGGCGC GGCAAGGGCG 5900
TGGTCGCCTA CACGCTGCTG TGCAACCACG TGCCGCTCAA CGGCTACCTG ATCGGCGCGC ACGATTACGA GGCCCATCAC GTGTTCGACA TCTGGTATCG 6000
CAACACGTCG GACATCGTGC CGACCGCGAT CACCGGCGAC ATGCACAGCG TCAACAAGGC CAACTTCGCT ATCCTGCACT GGTTCGGCCT GCGTTTCGAG 6100
CCGCGCTTCA CCGACCTTGG CGATCAGTTG AAGGAACTCT ACAGTGCCGA CGATCCGGCG CTGTACGATC AGTGCCTGAT CCGGCCGGCC GGGAGAATCG 6200
ACCGCGATCT CATAGTCAGC GAGAAGCCGA ACCTCGACCA GATTGTCGCC ACGCTCGGAC TGAAGGAGAT GACGCAGGGC ACGCTGATCC GCAAGCTATG 6300
CACCTACACC GCGCCGAACC CCACGCGGCG CGCGGTGTTC GAGTTCGACA AGCTCATCCG CAGCATCTAC ACGCTGCGCT ACCTGCGCGA TCCGCAACTG 6400
GAGCGCAACG TTCACCGCTC ACAGAACCGC ATCGAGTCCT ATCACCAGCT ACGCTCAACC ATCGCCCAGG TCGGCGGCAA GAAGGAATTG ACCGGGCGCA 6500
CCGACATCGA AATTGAGATC AGCAACCAGT GCGCCAGGCT GATCGCCAAC GCGGTCATCT TCTACAACTC GGCCATCCTC TCGCGGCTGC TGATGAAGTA 6600
CGAGGCGAGC GGCAACGCCA AGGCGCACGC TCTCCTGACC CAGATATCGC CGGCGGCCTG GCGGCACATC CTGCTGAACG GGCATTACAC CTTCCAGAGC 6700
GACGGCAAGA TGATCGACCT GGATGCGCTC GTGGCGGGGC TGGAGCTGGG ATGACGGAAA TTTCGGCGGT TCTGGCTTAG AACCCC

 Recombination Sites     

Name Coordinates Gene Sequence
res 2997-3127 131 GCCTGTCGGA AAACATTTGT TTTTCGACAG GCCTTCAACG GTCCTCTGCA CCAACCTCCG
AGTGGCCGCA AAATTGTGCG GAAAACTCTG TCGCCAGACG CTACCATACG GAAACCTCGT
CTTAATGGTT T
res_site_I 2997-3025 29 GCCTGTCGGA AAACATTTGT TTTTCGACA
res_site_II 3059-3102 44 TGGCCGCAAA ATTGTGCGGA AAACTCTGTC GCCAGACGCT ACCA
res_site_III 3103-3127 25 TACGGAAACC TCGTCTTAAT GGTTT

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
lysR family Tn5501.10 376-1239 Passenger Gene Other -
PAU-1 Tn5501.10 1307-2218 Passenger Gene Antibiotic Resistance +
parE Tn5501.10 2337-2621 Passenger Gene Toxin -
parD Tn5501.10 2618-2896 Passenger Gene Antitoxin -
tnpR Tn5501.10 3150-3728 Accessory Gene Resolvase +
tnpA Tn5501.10 3725-6754 Transposase   +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
lysR family LysR family Tn5501.10 864 376-1239 -
Class:   Passenger Gene
Sub Class:   Other
Protein Sequence:  
MDLPLNALRA FEVSARHLSF TRAGLELHLT QTAVSQHVKN LEDRLGKKLF RRIPRGLALT DEGAALLPVL TDAFERIGLT LEKVKTPHKK EVLSVGVVGT
FAVGWLLPRL RDFQVKHPFI DLRLFSNNNR VDLAGDGLDF AIRFGDGHWH GTEASKLFSA PLAPVCAPFM ASRIHTPSDL ARVTLLRSYR ADEWTAWFQA
VGLNPPHLTG PVFDSSLVLA DAAAQGTGVA LLPIRLFGRD LLGGRLVTLF DTRIETGSYW LTKLKPRKET DGMKAFRGWL EQECRDN

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
PAU-1 PAU-1 Tn5501.10 912 1307-2218 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Target:   piperacillin (ARO:0000078)||ampicillin (ARO:3000637)
Sequence Family:  Beta-lactamase2 (Pfam:PF13354)
Comment:   no match in CARD||PMID:31824180
Protein Sequence:  
MITHFQTVGT MKRRNFSVGM AFSAFGFSAI FPKKSFALGE QGQMTAVFQQ LEAKAQGRLG VHVIDTATGH EFGYRSDELF MMLSSFKLLA SALVLARADR
GEESLTRRIR YRKQDLVPWS PVTEAYADGE GLTLAQLCHA TITTSDNTAG NLILASYGGP QALTQYARQL GDKITRLDRN EPDLNTRVEG GSLDTTSPRA
MAMTMNKLLL GDALSPLSRN LLRQWLLENT TGGKRLKAGT PADWTVGDKT GTNKTDANDI GILLPPQGAP VLVTAYLADS TASSQIKDAT LAEVGRLTSI
GIR

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
parE ParE Tn5501.10 285 2337-2621 -
Class:   Passenger Gene
Sub Class:   Toxin
Target:   DNA gyrase
Sequence Family:  ParE_toxin (Pfam:PF05016)
Protein Sequence:  
VRVVWTPEAQ QDRADVWDYI AADNPRAAAR MDEIFSDAAA RLIQHPMLGK PGKIPGTREL IPHESYRLVY QIDGETVWIL TLVHTARLWP PVRD

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
parD ParD Tn5501.10 279 2618-2896 -
Class:   Passenger Gene
Sub Class:   Antitoxin
Sequence Family:  parD (PDB:4Q2U)
Comment:   RelB
Protein Sequence:  
MSKQAVFTMK LEPELRAEFM AEAEAAHRPA SQVLRELMRE FVQRQRESRE YDEFLRRKVE AGRASMRAGL GRSNDEVEAE FAARRASVAS QA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpR TnpR Tn5501.10 579 3150-3728 +
Class:   Accessory Gene
Sub Class:   Resolvase
Transpoase Chemistry:   Serine
Sequence Family:  Serine Site-Specific Recombinase
Protein Sequence:  
MRVSSDSDRQ STNLQRDALL AVGVDARHLF EDHASGAKDD RAGLARALEF VRPGDVLVVW KLDRLGRSLS HLLAIVTSLK KKQVAFRSLT ENLDTTTPSG
EFLFQVFGAL AQYERALIQE RVVAGLAAAR KRGRIGGRPQ AITGEKLEAI VAALDGGMSK AAVCRNFGVK RTTLIETLAR VGWTGSRGAS SR

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA Tn5501.10 3030 3725-6754 +
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MTTKSERLTV LSDAEQEALY GLPDFDDAQR LEYLALTETE LALASSRPGL HAQVYCILQI GYFKAKHAFF RFDWSEVEHD CAFVLSRYFH GESFEHKPIS
KHEHYTQREW IADLFGYRPW AAEFLAQLAQ QAAQTVRRDV MPGFIAAELI VWLNEHKIIR PGYTTLQELV SEALSAERRR LAGLLSEVLD ESAKAALGRL
LVRDDTLSQL AALKQDAKDF GWRQMARERE KRATLEPLHR IAKALLPKLG VSQQNLLYYA SLANFYTVHD LRNLKADQTY LYLLCYAWVR YRQLSDNLVD
AMAYHMKQLE DESSAGAKQS FVAEQVRRQQ DTPQVGRLLS LYIDDSVPDP TPFGDVRQRA YKIMPRDTLQ TTAQRMSVKP VSKLALHWQA VDGLAERIRR
HLRPLYVALD LAGTDPGSPW LVALAWAKDV FAKQQRLSQR PLAECPAATL PKRLRPYLLT FDADGKPTDL HADRYEFWLY RQVRKRFQSG ELYLDDSLQH
RHFSDELVSL DEKAAVLAQI DIPFLRQPLD AQLDALATEL RAQWLAFNRE LKQGKLTHLE YDKDTQKLTW RKPKGENQKA REKAFYEQLP FCDVADVFRF
VNGQCQFLSA LTPLQPRYAK KVADADSLMA VIIAQTMNHG NQVMARTSDI PYHVLESAYQ QYLRHATLHA ANDCISNAIA ALPIFPYYSF DLDALYGAVD
GQKFGVERPT VKARHSRKYF GRGKGVVAYT LLCNHVPLNG YLIGAHDYEA HHVFDIWYRN TSDIVPTAIT GDMHSVNKAN FAILHWFGLR FEPRFTDLGD
QLKELYSADD PALYDQCLIR PAGRIDRDLI VSEKPNLDQI VATLGLKEMT QGTLIRKLCT YTAPNPTRRA VFEFDKLIRS IYTLRYLRDP QLERNVHRSQ
NRIESYHQLR STIAQVGGKK ELTGRTDIEI EISNQCARLI ANAVIFYNSA ILSRLLMKYE ASGNAKAHAL LTQISPAAWR HILLNGHYTF QSDGKMIDLD
ALVAGLELG

 References     

Xu T, Wang J, Ying J, Zhu T, Liu Y, Xu L, Li P, Li P, Ying J, Li K, Yi H, Lu J, Hu Y, Zhou T, Bao Q. Characterisation of a class 1 integron associated with the formation of quadruple bla(GES-5) cassettes from an IncP-1β group plasmid in Pseudomonas aeruginosa. Int J Antimicrob Agents. 2018 Oct;52(4):485-491. doi: 10.1016/j.ijantimicag.2018.07.002. Epub 2018 Jul 31. PubMed ID: 30012438