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Internal Transposable Elements | |
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| Name: Tn512 |
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| Family: Tn402 Group: Tn5053 |
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| Evidence of Transposition: Yes |
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| Host |
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| Host Organism: | Pseudomonas a | | |
| Place of Origin: | Melbourne, Australia | Date of Isolation: | 1991 |
| | Other Geographic Information: | Austin Hospital clinical isolate |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TGTCATTTTC AGAAGACGAC TGCACCAATT GACGGGGCGT AACGCCAGAT GTGCAGGCGA CTCCTGACAA CGCAATATCA GAAGTCATCT GCACCAATCT 100
CGACTATGCT CAATACTCGT GTGCACCAAA GCGAGGTGTG AGCATGGCGT CAGACACATC ATTGATTGCC GAGCAAGGCG TGGCCACCCT GCCCGATGCG 200
GCTTGGGCGC AGGCCCGGCA ACGGGCGGAA ATCATCGGGC CGCTGGCAGC GCTTGATGTG GTCGGGCATG AAGCCGCCGA TGCCGCTGCT CACGCGCTTG 300
GCCTGTCCAG GCGGCAGGTG TATGTCCTAA TCCGCCGTGC CCGGCAAGGT GCTGGGCTTG TGACGGACCT GGCTCGCAGC CGATCCGGCG GCGGAAAAGG 400
CAAGGGACGC TTGCCGGAAT CAGTTGAGCG CATCATCCGC GAGTTGCTGC AAAAGCGCTT CCTGACCAAG CAGAAGCGTA GCCTGGCAGC GTTCCACCGC 500
GAGGTCGCGC AGGCTTGCAA AGCGCAAAAG CTGCGGGCGC CGGCGCGCAA CACCGTGGCT CTGCGGATCG CCGGCCTCGA TCCGCTCAAG GCCACTCGCC 600
GCCGGGAAGG TCAGGATGCG TCCCGCAGCC TGCAAGGTGT CGGTGGTGAG CCTCCCGCCG TGACCGCGCC ACTGGAACAA GTGCAGATTG ATCACACGGT 700
CATCGACCTG ATCGTGGTGG ACGAGCGCGA CCGGCAACCG ATTGGCCGTC CGTATCTGAC CATCGCCATC GACGTGTTTA CCCGCTGCGT GCTCGGCATG 800
GTCGTCACGC TGGAAGCGCC GTCATCTGTT TCGGTCGGCC TGTGCCTTGT GCATGTCGCC TGCGACAAGC GTCCCTGGCT GGAGGGTCTG AACATAGAAA 900
TGGAGTGGCC GATGAGCGGC AAGCCCAGGC TGCTCTACCT GGACAACGCG GCCGAGTTCA AGAGCGAAGC GCTACGCCGA GGCTGCGAGC AGCATGGCAT 1000
CCGGCTTGAC TATCGCCCGC TCGGGCAGCC GCACTACGGC GGCATCGTGG AACGGATCAT CGGCACGGCG ATGCAGATGA TCCACGACGA ATTGCCAGGG 1100
ACGACCTTCT CCAACCCTGA CCAGCGCGGC GACTACGATT CCGAAAACAA GGCCGCCCTG ACGCTGCGTG AGCTGGAGCG CTGGCTCACA TTGGCGGTCG 1200
GCACCTACCA CGGCTCCGTG CACAACGGCC TGCTCCAGCC GCCGGCAGCG CGCTGGGCCG AAGCTATCGC GCGGACCGGC GTGCCAACCG TCATCACTCG 1300
CACCACGGCT TTTCTGGTCG ATTTTCTGCC CATCATCCGC CGCACGCTGA CCCGCACCGG CTTCGTCATC GACCACATCC ATTACTACGC CGATGCGCTC 1400
AAGCCGTGGA TAGCTCGGCG CGACCGCTTG CCTGCGTTCC TGATCCGGCG CGACCCGCGC GACATCAGCC GCATTTGGGT GCTGGAGCCG GAGGGGCAGC 1500
ACTATCTGGA AATTCCATAC CGCACCTTGT CGCACCCGGC TGTCACCCTC TGGGAACAAC GACAGGCGCT GGCGAAATTG CGGCAGCAAG GGCGCGAACA 1600
GGTGGATGAG TCGGCGCTGT TTCGCATGAT CGGCCAGATG CGCGAAATCG TGTCCACCGC GCAGAAAGCT ACGCGCAAGG CGCGGCGCGA CGCGGATCGA 1700
CGCCAGCATC TCAAGGCAAC GGCAGTTCTT TTCAAAACCA CGCCACCACC GGACGCGGAC ATGGCTGACC CGCAGGCAGA CAACCAGCCA CCTGCCAAAC 1800
CGTTCGACCA GATTGAGGAG TGGTAGCCGT GGAAGAATAT CCCATCATCG ACTTGTCCCA CCTGATGCCG GTGGCCCAGG GCTTGGCCCG TCTTCCGGCG 1900
GACGAACGCA TCCATCGCCT TCGCGCTGAC CGCTGGATCG GCTATCCGCG AGCAGTCGAG GCGCTGAATC GGCTGGAAGC CCTGTATGCG TGGCCGAACA 2000
AACAACGCAT GCCCAACCTG CTGTTGGTCG GTCCAACCAA CAACGGCAAG TCGATGATCG TCGAGAAATT CCGCCGCACC CATCCGGCCA GCTCCGACGC 2100
CGACCAGGAG CACATCCCAG TGTTGGTTGT GCAGATGCCG TCCGAGCCGT CGGTGATCCG CTTCTACATC GCGCTACTTG CCGCGATGGG CGCGCCATTG 2200
CGCCCGCGCC CACGGCTGCC GGAAATGGAG CAATTGGCGC TGGCACTGCT GCGCAAGGTC GGCGTGCGCA TGCTGGTGAT CGACGAGTTG CACAACGTCC 2300
TGGCCGGCAA CAGCGTCAAC CGCCGGGAAT TCCTCAATCT GTTGCGTTTC CTCGGCAACG AGCTGCGCAT CCCGCTGGTC GGGGTCGGCA CACGCGATGC 2400
CTACTTGGCG ATCCGCTCGG ACGACCAGTT GGAAAACCGC TTCGAGCCGA TGATGCTGCC GGTGTGGGAG GCCAACGACG ATTGCTGCTC ACTGCTGGCC 2500
AGCTTCGCGG CTTCACTCCC GCTGCGGCGA CCCTCGTCGA TTGCCACGCT GGATATGGCC CGCTACCTGC TCACGCGCAG CGAGGGCACC ATCGGCGAGC 2600
TGGCGCACCT GTTGATGGCG GCGGCCGTCG CTGCCGTGGA GAGCGGTGAG GAAGCGATCA ACCATCGCAC GCTCAGCATG GCCGATTACA CCGGTCCCAG 2700
CGAGCGGCGG CGGCAATTCG AGCGGGAACT GATGTGAAGC CAGCGCCACA CTGGCCACTG CATCCGGCTC CCAGGGAAGG CGAAGCCTTG TCTTCGTGGC 2800
TCAACCGCGT GGCCCTTTGC TATCACATGG AGGTGTCCGA GCTGCTGGAG CACGATCTTG GTCACGGCCA GGTTGATGAC CTGGACACCG CGCCACCACT 2900
GGCGCTGCTG GCGATGCTCT CCCAGCGGAG CGGCATCGAG CCGGACCGGC TGCGTTGCAT GAGTTTCGCC GGCTGGGTGC CTTGGCTACT GGACAGCCTT 3000
GATGATCAGA TTCCAGACGC ATTGGAAACC TATGCGTTCC AGCTCTCGGT GCTGCTGCCG AAACTCCGCC GTAGGACGCG ATCCATCACG AGCTGGCGTG 3100
CCTGGCTGCC CAGCCAGCCG ATACATCGCG CCTGCCCGCT CTGTCTGAAC GACCCGGCAA ACCAAGCCGT ACTGCTTGCA TGGAAGCTGC CCCTGATGCT 3200
GAGCTGCCCG CTGCATGGCT GCTGGCTGGA ATCCTATTGG GGCGTGCCTG GGCGGTTTCT CGGCTGGGAG AACGCCGACA CTGCGCCGCG CACCGCCAGC 3300
GACGCGATTG CGGTGATGGA CCGGCGCACC TGGCAGGCAC TGACGACCGG CCATGTGGAG CTGCCGCGCC GACGCATCCA CGCTGGATTG TGGTTTCGGC 3400
TACTACGCAC GCTGCTCGAT GAGCTGAACA CCCCGCTTTC GACGTGCGGA ACCTGCGCGG GGTATCTCCG CCAAGTCTGG GAAGGCTGCG GGCATCCGCT 3500
GCGTGCTGGG CAAAGTCTGT GGCGACCGTA TGAAACCCTG AACCCGGCAG TACGGTTGCA GATGCTGGAG GCGGCGGCAA CGGCAATCAG CTTGATTGAG 3600
GTGAGGGATA TAAGCCCGCC GGGCGAGCAT GCAAAGCTGT TCTGGTCCGA ACCCCAAACC GGTTTCACCA GTGGCCTGTC GGCGAAAACG CCGAAGCCCG 3700
AACCCGTCGA TCACTGGCAA CGGGCGGTCC AGGCTATCAA TGAGGCGATC ATTGAAGCGC GGCACAACCC CGAGACGGCT CGCTCGCTGT TCGCGTTGGC 3800
TTCCTATGGT CGGCGCGACC CCGCTTCCCA GGAACAGTTG CGCGCCACCT TTGCAAAGGA AGGCATCCCT CCGGAATTTC TGTCACATTA CGAGCCTGAT 3900
GGACCCTTTG CATGTCTTAG ACAGAGTGAC GGGTTAAATG ACAAATTTTG ACGACCAGAA CTTTCCGGCG CACACTGTCA CATAATCGAA CGTATATGTG 4000
ACAGGTACGA CATGCTGATA GGCTACATGC GGGTATCGAA GGCGGACGGC TCCCAGGCTA CCGATTTGCA GCGCGACGCG CTGATTGCCG CCGGGGTCGA 4100
TCCAGTACAT CTTTACGAGG ACCAGGCATC CGGCATGCGC GAGGATCGGC CCGGCTTGAC GAGCTGCCTG AAGGCGTTGC GAACTGGCGA CACACTGGTC 4200
GTGTGGAAAC TGGATCGGCT CGGACGCGAC CTGCGACATC TCATCAACAC CGTGCACGAC CTGACTGGGC GCGGCATCGG CTTGAAGGTA TTAACCGGGC 4300
ACGGCGCGGC CATCGACACC ACGACCGCCG CCGGCAAGCT GGTCTTTGGC ATCTTCGCCG CCCTGGCCGA GTTCGAGCGC GAGTTGATCG CGGAGCGCAC 4400
GATTGCCGGC CTAGCCTCGG CCCGCGCGCG CGGGCGGAAA GGCGGCCGGC CGTTCAAGAT GACCGCCGCC AAGCTGCGGC TGGCGATGGC GGCAATGGGT 4500
CAGCCAGAGA CCAAGGTCGG CGACCTGTGC CAGGAACTTG GCGTCACGCG GCAGACCCTG TATCGGCATG TTTCACCCAA GGGTGAGCTA CGTCCAGATG 4600
GCGAGAAGCT ACTCAGCCGA ATTTGATGCC GGCATGAGGC AACGTAGCGA CAGCGTGGTT TGTCTCAATG GGAAGCGCTC ATGATCGATC TATTAAGGGC 4700
CGCAGCAGTC GTGTCACAGA CAGGACGAAC AAACCGATCA GCGTGAGGGC TGCGATACCC CAGTACTCTC CGATGAACGC GCCGGCCGTC GTGCCGGCCA 4800
GCACAATGGC GAGAATCGGC AAATGGCAGG GACAGGTGAG CACGGCCAGC GCGCCCCACA GGTAGGCAGT GATCGTTTGG TGCTTCTCGG ACGGCAAGCG 4900
TTCGGGGCTG TTCATGGCAG ACTCTCCGCG TGCTGGGCCG GCTCGGTCGG CATGGTGACC AACTGCACCT CCAGATCGAC CATCGCTTCG CGCCGACGCT 5000
CGACGAACTG GCGCAGAACG ACAAGCTGCG CGGCCGTTTC ATCGCTGTCC GCAGCATCCA GCGCCCGGCA CAGCCGCGCC AGCGCGTACA GGCCGATGCC 5100
CGCCTCGAAG GCCGCCCGCA CGAAGCACAG CCGTTGCAAG GCGGCATCAT CGAACAGGCC ATAGCCGCCC GGGGTGCACG CCACCGGACG CAGCAATCCG 5200
CGCAGCAGGT AGTCGCGCAC GATATGCACG CTCACCCCGG CATCAAGGGC CAGCCGGGAC ACGGTGTAGG CGCTCATTGA AAACCTCCTT TTTTTATCCA 5300
GCGCAGCAGG AAAGCTGCTT CACGTCCTTG TTGAAGGTCT GCGCCGCAAG CTTCAACCCC TCGACCATTG TCAGGTAGGG GAACAACTGG TCGGCCAGTT 5400
CCTGCACCGT CATGCGGTTG CGGATGGCGA GCACCGCCGT CTGGATCAGT TCGCCCGCTT CCGGGGCCAC CGCCTGCACG CCGATGAGCC GTCCGCTACC 5500
TTCCTCGATG ACCAGCTTGA TGAAGCCGCG TGTGTCGAAG TTGGCAAGCG CTCGCGGAAC GTTGTCGAGT GTCAGCGTGC GACTGTCGGT CTCGATGCCA 5600
TCGTGGTGCG CTTCCGCCTC GCTGTAGCCC ACGGTGGCGA CTTGCGGGTC GGTGAACACC ACTGCCGGCA TCGCGGTCAG ATTGAGGGCT GCGTCGCCGC 5700
CGGTCATGTT GATCGCGGCA CGGGTGCCGG CGGCCGCTGC CACGTAGACG AACTGCGGCT GGTCGGTGCA GTCGCCGGCC GCGTAGATGT TCGGGTTGCT 5800
CGTGCGCATG CCTTGGTCGA TAACGATGGC CCCTTGCGCA TTGACAGTGA CCCCCGCCGC GTCCAGCGCG AGGCTGCGCG TATTCGGTGC CCGACCGGTG 5900
GCAACCAGCA ACTTGTCAGC GCGCAATTCA CCGTGTCCGG TGGTCAGCAC GAATTCGCCG TTCACATGGG CGACCTGGCT GGCTTGCGTG TGCTCCAGCA 6000
CCTCGATGCC CTCGGCGCGG AAAGCGGCTG TCACGGCCTC GCCGATGGCC GGGTCTTCCC GGAAGAACAA GGTGCTGCGT GCCAGGATCG TGACCTGGCT 6100
GCCGAGCCGG GCAAAGGCTT GCGCCAGTTC CAACGCCACC ACCGACGAAC CGATCACGGC CAGGCGTGCG GGAATGGTGT CGCTGACAAG CGCTTCGGTG 6200
GAAGTCCAGT AGGGTGACTC TTTCAGGCCC GGAATCGGCG GCACGGCCGG ACTGGCACCG GTGGCGACCA GGCAGCGGTC GAACGTTACC TCGCGCTCGC 6300
CACCCTCGTT CAAACGGACG ACCAGGCTCT GGTCGTCCTT GAAACGCGCT TCACCGTGCA AAACGGTGAT GGCTGGATTG CCGTCCAGGA TGCCTTCGTA 6400
TTTGGCGTGC CGCAGTTCAT CGACACGGGC CTGCTGCTGG GCCAGCAGTT TGCTGCGGTC AATCGCAGGC ACAGTTGCCG CAATACCGCC GTCGAACGGA 6500
CTTTCCCGGC GCAGATGGGC AATATGGGCA GCGCGGATCA TGATCTTGGA CGGCACACAG CCGATATTGA CGCAGGTGCC GCCGATGGTG CCGCGTTCGA 6600
TCAGCGTGAC CGTCGCGCCT TGCTCGACGG CCTTCAGCGC CGCCGCCATC GCGGCCCCGC CGCTGCCAAT GATGGCGATA TGCAAACCGG CGCCCTCAAG 6700
TGCATCACGG ATTTTTGGTT CATCTTTGAA ATCACCAACC CGGATCGAGC CTTGATAACC CAATGCGGCG ATGGCGGCCA GCAGTTGGTT GTGGCTCACG 6800
GCGGTGTCTG CCATGACTTG CGCGCGGCTT TCTGGATAGG ACACCACAGC GGCATTCACG CCGGGAATCT TTTCCAAAGC ATCTTTGACA TGGGTGGCGC 6900
AGGATGTGCA GGTCATGCCA TTCACGGTGA TTTCGGTCAT TTTTTTACTC CAATGAATTT CGGGGTGCAG CAGGCATCGG CTTGGCGTTT TCGTTGGATG 7000
GCGTAGATGG TCAAGCCGAT GAAAATCGCC AGCGCAGGCA GCAGCACATA GTCCAGATAG CCGGTCAGCG CGGACAAGCT GACCACACCG AGCAAAATGA 7100
CCAGAACAGG GGTGAAGCAA CACAGCGCCA CGAGGGTTGT GCCAATGATG CTGACCCGCA GCAGTGTCTT CGGGTCTTTC ATGATCAGTT CTTGACTGAT 7200
GATGGGTAGC CCGCATCCTC GGTAGCCTTG GTCAGTTTCT GCACGCTGGT CTTGGCATCA TCGAAGGTGA CCACCGCTTC GCGCGTCTCG AAGGTCACGT 7300
CAACTTTACT GACGCCATCG ACCTTGGAAA TCGCCTTCTT GACAGTGATC GGACAGGCCG AGCAGGTCAT GCCCGGTACG GACAGCGTAA CGGTCTGGGT 7400
GGCGGCCCAC ACGGGGGCAA CAACGGCAGC GAGGGCAAGG GCGGAAAGCA GCTTTTTCAT GGTGAACTCC TGTGATCAAT AGAAAAATGG CACGACGTAG 7500
GGAAATCCGA GCGCGACCAA AACCAGCACG GCCACGCCCC AGAAAATGAG CTTGTAAGTA GCTCGCACTT GGGGAATCGC GCAAACCTCA CCCGGTTTGC 7600
AGGCGGCTGA CGGCCGGTAG ATGCGCCGCC AGGCGAAGAA CAACGCCACC AGCGCCACGC CGATAAAGAT GGGGCGATAG GGTTCCAACA CCGTCAAGTT 7700
GCCGATCCAA GCGCCGCTGA ACCCCAAGGC GATCAGAACC AGCGGCCCGA GGCAGCAAGC CGAGGCGAGG ATGGCGGCCA GCCCGCCAGT GAAGAGCGCG 7800
CCGCGCCCGT TTTGAGGTTC AGACATACGT TTGTCCTTTC GAATCTGAAT TGGATAGCTT AAGCTTACTT CCGTAGTTAT GTACGGAGTC AAGCGATATG 7900
GAAAACAATT TGGAGAACCT GACCATTGGC GTTTTCGCCA GGACGGCCGG GGTCAATGTG GAGACCATCC GGTTCTATCA GCGCAAGGGC TTGCTCCCGG 8000
AACCGGACAA GCCTTACGGC AGCATTCGCC GCTATGGCGA GACGGATGTA ACGCGGGTGC GCTTCGTGAA ATCAGCCCAG CGGTTGGGCT TCAGCCTGGA 8100
TGAGATCGCC GAGCTGCTGC GGCTGGAGGA TGGCACCCAT TGCGAGGAAG CCAGCAGCCT GGCCGAGCAC AAGCTCAAGG ACGTGCGCGA GAGGATGGCT 8200
GACCTGGCGC GCATGGAGGC CGTGCTGTCT GATTTGGTGT GCGCCTGCCA TGCGCGGAAG GGGAACGTTT CCTGCCCGCT GATTGCGTCA CTGCAAGGGA 8300
AGAAAGAACC GCGCAGTGCG GACGCGGTGT AGCCCGAGGG AACTACGCCT TAGCGTGCTT TATTTTCCGT TTTCTGAGGC GACTCCAACG TCAGAAAAGA 8400
CCGTGCGGTC GACTTTTGAT ATTTCGTGCT GTCGCCTTCT GAAAGTGACA
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| Recombination Sites |
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| Name |
Coordinates |
Gene |
Sequence |
| repeat t3 |
78-97 |
20 |
TCAGAAGTCA TCTGCACCAA |
| r5 |
3875-3888 |
14 |
AATTTCTGTC ACAT |
| res |
3972-4006 |
35 |
ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 |
3975-3988 |
14 |
CTGTCACATA ATCG |
| r1 |
3991-4004 |
14 |
CGTATATGTG ACAG |
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tniA |
Tn512 |
144-1826 |
Transposase |
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+ |
| tniB |
Tn512 |
1829-2737 |
Accessory Gene |
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+ |
| tniQ |
Tn512 |
2734-3951 |
Accessory Gene |
Target Site Selection |
+ |
| tniR |
Tn512 |
4012-4626 |
Accessory Gene |
Resolvase |
+ |
| merE |
Tn512 |
4679-4915 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merD |
Tn512 |
4912-5277 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merA |
Tn512 |
5294-6940 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merF |
Tn512 |
6937-7182 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merP |
Tn512 |
7185-7460 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merT |
Tn512 |
7476-7826 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merR |
Tn512 |
7898-8332 |
Passenger Gene |
Heavy Metal Resistance |
+ |
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniA |
TniA |
Tn512 |
1683 |
144-1826 |
+ |
| Class: | Transposase |
| Transpoase Chemistry: | DDE |
| Comment: | homologous to TnsB of Tn7 |
Protein Sequence:
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MASDTSLIAE QGVATLPDAA WAQARQRAEI IGPLAALDVV GHEAADAAAH ALGLSRRQVY VLIRRARQGA GLVTDLARSR SGGGKGKGRL PESVERIIRE
LLQKRFLTKQ KRSLAAFHRE VAQACKAQKL RAPARNTVAL RIAGLDPLKA TRRREGQDAS RSLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDERDRQPI
GRPYLTIAID VFTRCVLGMV VTLEAPSSVS VGLCLVHVAC DKRPWLEGLN IEMEWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG
IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAIARTGV PTVITRTTAF LVDFLPIIRR
TLTRTGFVID HIHYYADALK PWIARRDRLP AFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR
EIVSTAQKAT RKARRDADRR QHLKATAVLF KTTPPPDADM ADPQADNQPP AKPFDQIEEW
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniB |
TniB |
Tn512 |
909 |
1829-2737 |
+ |
| Class: | Accessory Gene |
| Transpoase Chemistry: | Serine |
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein |
Protein Sequence:
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VEEYPIIDLS HLMPVAQGLA RLPADERIHR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV
VQMPSEPSVI RFYIALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ
LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSSIAT LDMARYLLTR SEGTIGELAH LLMAAAVAAV ESGEEAINHR TLSMADYTGP SERRRQFERE
LM
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniQ |
TniQ |
Tn512 |
1218 |
2734-3951 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Target Site Selection |
Protein Sequence:
|
MKPAPHWPLH PAPREGEALS SWLNRVALCY HMEVSELLEH DLGHGQVDDL DTAPPLALLA MLSQRSGIEP DRLRCMSFAG WVPWLLDSLD DQIPDALETY
AFQLSVLLPK LRRRTRSITS WRAWLPSQPI HRACPLCLND PANQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADTAPRTASD AIAVMDRRTW
QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSTCGT CAGYLRQVWE GCGHPLRAGQ SLWRPYETLN PAVRLQMLEA AATAISLIEV RDISPPGEHA
KLFWSEPQTG FTSGLSAKTP KPEPVDHWQR AVQAINEAII EARHNPETAR SLFALASYGR RDPASQEQLR ATFAKEGIPP EFLSHYEPDG PFACLRQSDG
LNDKF
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniR |
TniR |
Tn512 |
615 |
4012-4626 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Resolvase |
| Transpoase Chemistry: | Serine |
| Sequence Family: | Serine Site-Specific Recombinase |
| Comment: | also called TniC |
Protein Sequence:
|
MLIGYMRVSK ADGSQATDLQ RDALIAAGVD PVHLYEDQAS GMREDRPGLT SCLKALRTGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA
IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGVTR QTLYRHVSPK GELRPDGEKL
LSRI
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merE |
MerE |
Tn512 |
237 |
4679-4915 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | Broad-spectrum mercury transporter |
Protein Sequence:
|
MNSPERLPSE KHQTITAYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE YWGIAALTLI GLFVLSVTRL LRPLIDRS
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merD |
MerD |
Tn512 |
366 |
4912-5277 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
Protein Sequence:
|
MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLYALARLC RALDAADSDE TAAQLVVLRQ FVERRREAMV
DLEVQLVTMP TEPAQHAESL P
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merA |
MerA |
Tn512 |
1647 |
5294-6940 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Function: | mercuric ion reductase |
| Target: | Mercury |
Protein Sequence:
|
MTEITVNGMT CTSCATHVKD ALEKIPGVNA AVVSYPESRA QVMADTAVSH NQLLAAIAAL GYQGSIRVGD FKDEPKIRDA LEGAGLHIAI IGSGGAAMAA
ALKAVEQGAT VTLIERGTIG GTCVNIGCVP SKIMIRAAHI AHLRRESPFD GGIAATVPAI DRSKLLAQQQ ARVDELRHAK YEGILDGNPA ITVLHGEARF
KDDQSLVVRL NEGGEREVTF DRCLVATGAS PAVPPIPGLK ESPYWTSTEA LVSDTIPARL AVIGSSVVAL ELAQAFARLG SQVTILARST LFFREDPAIG
EAVTAAFRAE GIEVLEHTQA SQVAHVNGEF VLTTGHGELR ADKLLVATGR APNTRSLALD AAGVTVNAQG AIVIDQGMRT SNPNIYAAGD CTDQPQFVYV
AAAAGTRAAI NMTGGDAALN LTAMPAVVFT DPQVATVGYS EAEAHHDGIE TDSRTLTLDN VPRALANFDT RGFIKLVIEE GSGRLIGVQA VAPEAGELIQ
TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFNKDV KQLSCCAG
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merF |
MerF |
Tn512 |
246 |
6937-7182 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | mercuric ion transport protein |
Protein Sequence:
|
MKDPKTLLRV SIIGTTLVAL CCFTPVLVIL LGVVSLSALT GYLDYVLLPA LAIFIGLTIY AIQRKRQADA CCTPKFIGVK K
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merP |
MerP |
Tn512 |
276 |
7185-7460 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | periplasmic mercuric ion binding protein |
Protein Sequence:
|
MKKLLSALAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISKVDGV SKVDVTFETR EAVVTFDDAK TSVQKLTKAT EDAGYPSSVK N
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merT |
MerT |
Tn512 |
351 |
7476-7826 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | mercury ion transport protein |
Protein Sequence:
|
MSEPQNGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLTVLEPY RPIFIGVALV ALFFAWRRIY RPSAACKPGE VCAIPQVRAT YKLIFWGVAV
LVLVALGFPY VVPFFY
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merR |
MerR |
Tn512 |
435 |
7898-8332 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
Protein Sequence:
|
MENNLENLTI GVFARTAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGETD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVRERM
ADLARMEAVL SDLVCACHAR KGNVSCPLIA SLQGKKEPRS ADAV
|
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| Internal Repeat Elements |
|
|
| Name |
Associated Mobile Element |
Coordinates |
Sequence (Top Strand) |
| repeat t2 |
Tn512 |
49-67 |
TACACGTCCG CTGAGGACT |
| repeat t4 |
Tn5053 |
110-128 |
TCAATACTCG TGTGCACCA |
| IR Tn21-like |
Tn512 |
8349-8386 |
GAATCGCACG AAATAAAAGG CAAAAGACTC CGCTGAGG |
| repeat i4 |
Tn512 |
8359-8377 |
AAATAAAAGG CAAAAGACT |
| repeat i2 |
Tn512 |
8401-8419 |
CCGTGCGGTC GACTTTTGA |
| IRi |
Tn512 |
8423-8450 |
AAGCACGACA GCGGAAGACT TTCACTGT |
|
| References |
|
|
Petrovski S, Stanisich VA. Tn502 and Tn512 are res site hunters that provide evidence of resolvase-independent transposition to random sites. J Bacteriol. 2010 Apr;192(7):1865-74. doi: 10.1128/JB.01322-09. Epub 2010 Jan 29. PubMed ID: 20118251
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