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| Name: Tn5053.4 |
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| Family: Tn402 Group: Tn5053 |
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| Evidence of Transposition: Yes |
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| Host |
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| Host Organism: | Enterobacter cloacae subsp. cloacae ATCC 13047 | Molecular Source: | plasmid pECL_A |
| Place of Origin: | Tianjin, China | Date of Isolation: | 2010 |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TGTCGTTTTC AGAAGACGAC CGCACCATCT GACTGGATGT AACGCCTGGT GTGCATACGG CTCCTGACAG CCCAATATCA GGAGTCGTCT GCACCAATCT 100
CGACTATGCT CAATACTCGT GTGCACCAAA GCGAGGTTTG GGCATGACAT CAGACACTCC ACCGATTGCC GCGCAAGGCG TGGCCACCCT GCCCGACGAG 200
GCATGGGCGC AAGCCCGGCA CCGGACGGAA ATCATCGGGC CACTGGCAGC GCTTGAGGTG GTCGGGCATG AAGCCGCCGA TGAGGCAGCC CAAGCGCTGG 300
GCCTGTCCCG GCGACAGGTA TATGTCCTGA TCCGTCGCGC CCGGCAGGGT ACTGGCCTGG TAACAGACCT GACGCCCGGC CGATCCGGCG GCGGCAAAGG 400
CAAGGGGCGC TTGCCGGAAC CGGTCGAGCG CATCATCCGC GAGCTGCTGC AAAAGCGCTT CCTGACCAAG CAGAAACGCA GCCTGGCGGC GTTCCACCGC 500
GAAGTCGCGC AGGCGTGCAA AACCCAGAAG CTGCCGGTGC CGGCGCGCAA CACCGTGGCC CAGCGGATTG CCGGACTACA TCCGGCGAAA ATAGCCCGCA 600
GCCGGGGCGG GCAGGACGCT GCCCGTCCCT TGCAAGGCGC GGGTGGCATT CCGCCCGAAG TCACCATGCC GCTGGAACAG GTGCAGATCG ACCACACCGT 700
CATCGACCTG ATCGTGGTCG ACGAGCGCGA CCGGCAACCG ATTGGCCGCC CATATTTGAC CCTCGCCATC GACGTGTTCA CGCGCTGCGT ACTCGGCATG 800
GTGGTCACGC TGGAAGCGCC GTCCGCCGTC TCGGTCGGCC TATGCCTCGC GCATGCCGCC TGCGACAAGC GGCCCTGGCT GGAAGGGCTG AATGTGGAAA 900
TGGACTGGCC GATGAGCGGC AAGCCCAGGC TGCTCTATCT GGACAACGCG GCCGAGTTCA AAAGCGAAGC GCTGCGCCGT GGCTGCGAAC AGCATGGCAT 1000
CCGGCTGGAC TATCGCCCAC CAGGCCAGCC GCACTACGGC GGCATCGTGG AACGGATCAT CGGCACGGCG ATGCAGATGA TCCACGACGA ATTACCGGGG 1100
ACGACCTTCT CCAATCCCGG CCAGCGCGGC GAGTACGATT CCGAGAAGAT GGCCACCCTG ACGCTGCGCG AGCTGGAGCG CTGGCTCGCG TTGGCGGTAG 1200
GCACCTATCA CGGCTCCGTG CACAACGGCC TGCTCCAGCC GCCGGCCGCG CGCTGGGCCG AGGCCGTGGA GCGCGTTGGC GTCCCGGCCG TCGTTACCCG 1300
CCCCACCGCG TTTTTGGTCG ATTTCCTGCC GGTGATCCGC CGCACCCTGA CCCGCACCGG CTTTGTCATC GACCACATCC ACTACTACGC CGACGCCCTC 1400
AAGCCGTGGA TTGCCCGGCG CGAGCGCTTG CCCGCCTTCC TGATCCGGCG CGATCCGCGC GACATCAGCC GCATCTGGGT ACTGGAACCG GAAGGTCAGC 1500
ACTATCTGGA GATCCACTAC CGCACCTTGT CCCATCCGGC CGTCACCCTC TGGGAACAAC GCCAGGCGCT GGCCAAATTG CGTCAGCTCG GGCGCGAGCA 1600
GGTGGACGAG TCGGCGCTGT TCCGCATGAT CGGGCAGATG CGCGAGATCG TGACCACCGC CCAGAAGGCC ACGCGCAAGG CGCGGCGCGA CGCTGATCGC 1700
CGCCAGCACC TCAAGACGTC GGAGCCACCG GCCAAGCCCA TACCGCCGGA TGTGGACATG GCTGACCCGC AGGCAGACAA CCTGCCGCCG GCCAAACCGT 1800
TCGATCAGAT CGAGGAGTGG TAGCCGTGGA CGAATATCCC GTCATTGACC TGTCCCACCT GCTGCCAGCG GCACAGGGTT TGGCCAGGCT GCCGGCAGAC 1900
GAGCGCATCC AGCGCATTCG CGCCGACCGC TGGATCGGCT ACCCGCGCGC GGTCGAGGCG CTGAACCGGC TGGAAACTCT GTATGCGTGG CCGAACAAGC 2000
AACGCATGCC AAACCTGCTG CTGGTCGGCC CCACCAACAA CGGCAAGTCG ATGATCGTCG AGAAATTCCG GCGGGCGCAC CCGGTCGGCA CCGACGCTGA 2100
CCAAGAACAT ATCCCGGTGC TGGTCGTGCA GATGCCGTCA GAGCCATCGG TGATCCGCTT CTATGTCGCG CTGCTGGCTG CGATGGGCGC GCCGCTGCGC 2200
CCGCGCCCAC GGCTGCCGGA AATGGAGCAA CTGGCGCTGG CCCTGCTGCG CAAGGTCGGC GTGCGCATGC TGGTGATCGA CGAACTGCAC AATGTACTGG 2300
CTGGCAACAG CGTTAACCGG CGCGAGTTCC TCAACCTGCT GCGCTTCCTC GGCAACGAGC TGCGTATCCC CCTGGTCGGG GTCGGCACGC GCGAGGCGTA 2400
CCTGGCCATC CGTTCGGACG ATCAGTTGGA AAACCGCTTC GAGCCGATGC TGCTGCCGCC GTGGGAGGCC AATGAGGACT GCTGCTCGCT GCTGGCCAGC 2500
TTCGCGGCGT CACTCCCGCT ACGGCGGCCA TCCCCGATTG CCACGCTGGA CATGGCCCGC TACCTGCTCA CCCGGAGCGA AGGCACCATC GGCGAGCTTG 2600
CACATCTGCT GGTGGCGGCG GCCGTCGCCG CCGTGGAGAG TGGCGAGGAA GCGATCAACC ACCGCACGCT CAGCATGGCC GACTACATCG GCCCCAGTGA 2700
GCGGCGGCGA CAGTTCGAGC GGGAACTGAT GTGAAGTCCG CGCCGCGCTG GCCGCTGCAT CCGGCACCCA AGGAAGGCGA AGCCCTGTCC TCATGGCTCA 2800
ACCGGGTCGC CGCCTGCTAC CAGATGGACG TGCACGAGCT GCTGGCCCAC GATCTTGGTC ACAGCCAACT TGATGACCTG GATACCGCAC CATCCTTGTC 2900
GTTGCTGACG GCGCTCTGCC AGCGCAGTGG CGTCGAGCTG GAGCGGTTGC GCAGCATGAG TCTTGCAGGC TGGGTGCCGT GGCTGCTCGA CAGTCTCGAC 3000
GATTCGGTAC CAGCAGCCTT GGAAACCTAT ACATTCCAAT GCGCGGTGCT CCTGCCCAAG CGCACCCGCA AGGTGCGGTC CATCACTCGC TGGCGTGCCT 3100
GGCTACCGAG CCAGACGATT CGCCGGGCGT GTCCGCAGTG TCTGAACGAT CCAACGAATC AAGCTGTGCT ACTCGTCTGG CAGCTCCCCT TGATGCTGAG 3200
CTGCCCGCAG CATGGCTGCT GGCTGGAGTC CTACTGGGGC ATGCCCGGCC GGTATCTTCA GTGGGAAATC GCCGATGCTG CGCCGCGCCC TGCCGATGAC 3300
GCAATCGCCT GCATGGACCG GCGCACCTGG CAGGCGCTGA CGACGGGTTT TGTGGAGCTG CCGCGTCGGC GTGTCCACGC CGGCTTGTGG TTCCGGCTGC 3400
TGCGCACCCT GCTTGATGAG CTGAACACGC CGCTCTCGCA CTGCGGCAGT TGCTCGGCGA GCATCCGCCA TGTCTGGGAG CGCTGCGGCC ATCCGCTGCG 3500
CGCCGGGCAG AGTCTGTGGC GTCCCTACGA GATTCTGGCC CCGGCGGTGC AGTGGCAGAT GCTGGAGGCG GCGGCCACCG CCATCACGCT GATCGAGTCA 3600
AAGGCGCTGA TCCCGCGCGG GGAACAGGCC GCGCTGTTTC AGACGGAGCC GTACACTGGT TTCACCAACG GCCTGCCGGC GAAGGTGCCG AAGCCGGAAC 3700
CCATCAACCA CTGGCAACGA GCAGCCCAGG CCATCGATGA GGCCATCATT GAAGCGCGAC ACAACCCGGA GACGGCCCGC TTGCTGTTCA CACTGGCGTC 3800
CTACGGGCGA CGCGACCCCG AATCCCTGGA ACGTTTGCGC GCCACGTTCA CCAAGGAGGG CATCCCGCCG GAGTTTTTGT CACATTACGA GCCTGAATGG 3900
CCGTTTGCAG GTCTTAGACT AAATGACGGG TTAAGTGACA GTTTTTGACG GCGAGAGCTT TCTGGCTCAC ACTGTCACAT AATCGAACGT ATATGTGACA 4000
GGTACGACAT GCTGATAGGC TACATGCGGG TATCGAAGGC GGACGGCTCC CAGGCTACCG ATTTGCAGCG CGACGCGCTG ATTGCCGCCG GGGTCGATCC 4100
AGTACATCTT TACGAGGACC AGGCATCCGG CATGCGCGAG GATCGGCCCG GCTTGACGAG CTGCCTGAAG GCGTTGCGAA CTGGCGACAC ACTGGTCGTG 4200
TGGAAACTGG ATCGGCTCGG ACGCGACCTG CGACATCTCA TCAACACCGT GCACGACCTG ACTGGGCGCG GCATCGGCTT GAAGGTATTA ACCGGGCACG 4300
GCGCGGCCAT CGACACCACG ACCGCCGCCG GCAAGCTGGT CTTTGGCATC TTCGCCGCCC TGGCCGAGTT CGAGCGCGAG TTGATCGCGG AGCGCACGAT 4400
TGCCGGCCTA GCCTCGGCCC GCGCGCGCGG GCGGAAAGGC GGCCGGCCGT TCAAGATGAC CGCCGCCAAG CTGCGGCTGG CGATGGCGGC AATGGGTCAG 4500
CCAGAGACCA AGGTCGGCGA CCTGTGCCAG GAACTTGGCG TCACGCGGCA GACCCTGTAT CGGCATGTTT CACCCAAGGG TGAGCTACGT CCAGATGGCG 4600
AGAAGCTACT CAGCCGAATT TGATGCCGGC ATGAGGCAAC GTAGCGACAG CGTGGTTTGT CTCAATGGGA AGCGCTCATG ATCGATCTTT GAAGGCCCGC 4700
AGCAGTCGTG TCACAGACAG GACGAACAAA CCGGTCAGCG TGAGGGCTGC GATACCCCAG TACTCTCCGA TGAACGCGCC GGCCGTCGTG CCGGCCAGCA 4800
CAATGGCGAG AATCGGCAAA TGGCAGGGAC AGGTGAGCAC GGCCAGCGCG CCCCACAGGT AGCCGGTGAT CGGTTTGTGC GTCTCGGACG GCAAGCGCTC 4900
GGGGCTGTTC ATGGCAGACT CTCCGCGTGC TGTGCCGGCT CGGTCGGCAT GGTGGCCAAC TGCACCTCCA GATCGGCCAA CGCTTCGCGC CGACGCTCGA 5000
CGAACTGGCG CAGAACGGCA AGCTGCGCGG CCGCTTCATC GCCGTCCGCA GCATCCAGCG CCCGGCACAG CCGCGCCAGC GCGTCCAGGC CGATGCCCGC 5100
CTCGAAGGCC GCCCGCACGA AGCACAGCCG TTGCAAGGCG GCATCATCGA ACAGGCCATA GCCGCCCGGG GTGCACGCCA CCGGACGCAG CAATCCGCGC 5200
AGCAGGTAGT CGCGCACGAT ATGCACGCTC ACCCCGGCAT CAAGGGCCAG CCGGGACACG GTGTAGGCGC TCATTGAAAA CCTCCTTTTT TTATCCAGCG 5300
CAGCAGGAAA GCTGCTTCAC GTCCTTGTTG AAGGTCTGCG CCGCAAGCTT CAACCCCTCG ACCATTGTCA GGTAGGGGAA CAACTGGTCG GCCAGTTCCT 5400
GCACCGTCAT GCGGTTGCGG ATGGCGAGCA CCGCCGTCTG GATCAGTTCG CCCGCTTCCG GGGCCACCGC CTGCACGCCG ATGAGCCGTC CGCTACCTTC 5500
CTCGATGACC AGCTTGATGA AGCCGCGTGT GTCGAAGTTG GCAAGCGCTC GCGGAACGTT GTCGAGTGTC AGCGTGCGAC TGTCGGTCTC GATGCCATCG 5600
TGGTGCGCTT CCGCCTCGCT GTAGCCCACG GTGGCGACTT GCGGGTCGGT GAACACCACT GCCGGCATCG CGGTCAGATT GAGGGCTGCG TCGCCGCCGG 5700
TCATGTTGAT CGCGGCACGG GTGCCGGCGG CCGCTGCCAC GTAGACGAAC TGCGGCTGGT CGGTGCAGTC GCCGGCCGCG TAGATGTTCG GGTTGCTCGT 5800
GCGCATGCCT TGGTCGATAA CGATGGCCCC TTGCGCATTG ACAGTGACCC CCGCCGCGTC CAGCGCGAGG CTGCGCGTAT TCGGTGCCCG ACCGGTGGCA 5900
ACCAGCAACT TGTCAGCGCG TAATTCACCG TGTCCGGTGG TCAGCACGAA TTCGCCGTTC ACATGGGCGA CCTGGCTGGC TTGCGTGTGC TCCAGCACCT 6000
CGATGCCCTC GGCGCGGAAA GCGGCTGTCA CGGCCTCGCC GATGGCCGGG TCTTCCCGGA AGAACAAGGT GCTGCGTGCC AGGATCGTGA CCTGGCTGCC 6100
GAGCCGGGCA AAGGCTTGCG CCAGTTCCAA CGCCACCACC GACGAACCGA TCACGGCCAG GCGTGCGGGA ATGGTGTCGC TGACAAGCGC TTCGGTGGAA 6200
GTCCAGTAGG GTGACTCTTT CAGGCCCGGA ATCGGCGGCA CGGCCGGACT GGCACCGGTG GCGACCAGGC AGCGGTCGAA CGTTACCTCG CGCTCGCCAC 6300
CCTCGTTCAA ACGGACGACC AGGCTCTGGT CGTCCTTGAA ACGCGCTTCA CCGTGCAAAA CGGTGATGGC TGGATTGCCG TCCAGGATGC CTTCGTATTT 6400
GGCGTGCCGC AGTTCATCGA CACGGGCCTG CTGCTGGGCC AGCAGTTTGC TGCGGTCAAT CGCAGGCACA GTTGCCGCAA TACCGCCGTC GAACGGACTT 6500
TCCCGGCGCA GATGGGCAAT ATGGGCAGCG CGGATCATGA TCTTGGACGG CACACAGCCG ATATTGACGC AGGTGCCGCC GATGGTGCCG CGTTCGATCA 6600
GCGTGACCGT CGCGCCTTGC TCGACGGCCT TCAGCGCCGC CGCCATCGCG GCCCCGCCGC TGCCAATGAT GGCGATATGC AAACCGGCGC CCTCAAGTGC 6700
ATCACGGATT TTTGGTTCAT CTTTGAAATC ACCAACCCGG ATCGAGCCTT GATAACCCAA TGCGGCGATG GCGGCCAGCA GTTGGTTGTG GCTCACGGCG 6800
GTGTCTGCCA TGACTTGCGC GCGGCTTTCT GGATAGGACA CCACAGCGGC ATTCACGCCG GGAATCTTTT CCAAAGCATC TTTGACATGG GTGGCGCAGG 6900
ATGTGCAGGT CATGCCATTC ACGGTGATTT CGGTCATTTT TTTACTCCAT TGAATTTCGG GGTGCAGCAG GCATCGGCTT GGCGTTTTCG TTGGATGGCG 7000
TAGATGGTCA AGCCGATGAA AATCGCCAGC GCAGGCAGCA GCACATAGTC CAGATAGCCG GTCAGCGCGG ACAAGCCGAC CACACCGAGC AAAATGACCA 7100
GAACAGGGGT GAAGCAACAC AGCGCCACGA GGGTTGTGCC AATGATGCTG ACCCGCAGCA GTGTCTTCGG GTCTTTCATG ATCAGTTCTT GACTGATGAT 7200
GGGTAGCCCG CATCCTCGGT AGCCTTGGTC AGTTTCTGCA CGCTGGTCTT GGCATCATCG AAGGTGACCA CCGCTTCGCG CGTCTCGAAG GTCACGTCAA 7300
CTTTACTGAC GCCATCGACC TTGGAAATCG CCTTCTTGAC AGTGATCGGA CAGGCCGAGC AGGTCATGCC CGGTACGGAC AACGTAACGG TCTGGGTGGC 7400
GGCCCACACG GGGGCAACAA CGGCAGCGAG GGCAAGGGCG GAAAGCAGCT TTTTCATGGT GAACTCCTGT GATCAATAGA AAAATGGCAC GACGTAGGGA 7500
AATCCGAGCG CGACCAAAAC CAGCACGGCC ACGCCCCAGA AAATGAGCTT GTAAGTAGCT CGCACTTGGG GAATCGCGCA AACCTCACCC GGTTTGCAGG 7600
CGGCTGACGG CCGGTAGATG CGCCGCCAGG CGAAGAACAA CGCCACCAGC GCCACGCCGA TAAAGATGGG GCGATAGGGT TCCAACACCG TCAAGTTGCC 7700
GATCCAAGCG CCGCTGAACC CCAAGGCGAT CAGAACCAGC GGCCCGAGGC AGCAAGCCGA GGCGAGGATG GCGGCCAGCC CGCCAGTGAA GAGCGCGCCG 7800
CGCCCGTTTT GAGGTTCAGA CATACGTTTG TCCTTTCGAA TCTGAATTGG ATAGCTTAAG CTTACTTCCG TAGTTATGTA CGGAGTCAAG CGATATGGAA 7900
AACAATTTGG AGAACCTGAC CATTGGCGTT TTCGCCAGGA CGGCCGGGGT CAATGTGGAG ACCATCCGGT TCTATCAGCG CAAGGGCTTG CTCCCGGAAC 8000
CGGACAAGCC TTACGGCAGC ATTCGCCGCT ATGGCGAGAC GGATGTAACG CGGGTGCGCT TCGTGAAATC AGCCCAGCGG TTGGGTTTCA GCCTGGATGA 8100
GATCGCCGAG CTGCTGCGGC TGGAGGATGG CACCCATTGC GAGGAAGCCA GCAGCCTGGC CGAGCACAAG CTCAAGGACG TGCGCGAGAG GATGGCTGAC 8200
CTGGCGCGCA TGGAGGCCGT GCTGTCTGAT TTGGTGTGCG CCTGCCATGC GCGGAAGGGG AACGTTTCCT GCCCGCTGAT TGCGTCACTG CAAGGGAAGA 8300
AAGAACCGCG CAGTGCGGAC GCGGTGTAGC CCGAGGGAAC TACGCCTTAG CGTGCTTTAT TTAATGAGAT GGTCACTCCC TCCTTCCCGG TACTATGCTG 8400
AGGACAGGCT TTCATTCGGA GAACTATCAT GGAAAACATT GCGCTCATTG GTATCGATCT GGGTAAAAAC TCTTTCCATA TTCATTGCCA GGATCGTCGC 8500
GGGAAGGCTG TTTACCGTAA AAAATTTACC CGGCCAAAGT TGATCGAATT TTTGGCGACA TGCCCCGCTA CAACCATCGC AATGGAAGCC TGTGGCGGTT 8600
CTCACTTTAT GGCACGCAAG TTGGAAGAGT TGGGGCATTC CCCAAAGCTG ATATCACCAC AATTTGTCCG CCCGTTCGTT AAAAGCAATA AAAACGACTT 8700
TGTCGACGCC GAAGCTATTT GTGAAGCTGC ATCGCGTCCG TCTATGCGTT TTGTGCAGCC CAGAACGGAA TCTCAGCAGG CAATGCGGGC TCTGCATCGT 8800
GTCCGTGAAT CCCTGGTTCA GGATAAGGTA AAAACAACCA ATCAAATGCA TGCTTTTCTG CTGGAATTTG GCATTAGCGT TCCCCGAGGA GCTGCCGTTA 8900
TTAGCCGACT GAGTACCATT CTTGAGGATA ATAGTTTGCC TCTTTACCTC AGCCAGTTAT TGCTGAAATT ACAACAGCAT TATCACTATC TTGTGGAGCA 9000
GATTAAAGAT TTGGAATCCC AGTTGAAACG AAAGTTGGAC GAAGATGAGG TTGGACAGCG CTTGCTGAGC ATTCCCTGCG TCGGAACACT GACAGCGAGT 9100
ACTATTTCAA CTGAGATTGG CGACGGGAAG CAGTACGCCA GCAGCCGTGA CTTTGCGGCG GCAACAGGGC TTGTACCTCG GCAGTACAGC ACGGGAGGTA 9200
GGACGACATT GCTGGGAATT AGTAAGCGAG GTAATAAAAA GATCCGAACT TTGTTGGTTC AATGTGCCAG GGTATTCATA CAAAAACTGG AACACCAGTC 9300
TGGCAAATTG GCCGATTGGG TCAGGGATTT ACTGTGCCGG AAAAGCAACT TTGTCGTCAC TTGTGCTCTG GCAAACAAGC TGGCCAGAAT AGCCTGGGCC 9400
CTAACGGCAC GACAGCAAAC TTATGTAGCA TAACGGCAGA AATACACCGG TTTAAAGAAT TACTGATCTG GTTTTGCGAA TACTGATATT GATGATACTA 9500
ACGGCCCACC GGCCTGTTGA GGAACCTGTA AAACGGAAAG GCTCATTGAA GCCGTATATT TTCTGGAGGT TCATCAGGCG CGGAACTCAT CAAGGCGCGG 9600
GAATAAAATC CCATTCAGAC GCCGGATAGA TTCAAGCAAG CCAACTTGTC GTCAAAATCG GTGTTGCAAA AACGGGAGTG ACCATAGATT CCGTTTTCTG 9700
AGGCGACTCC AACGTCAGAA AAGACCGTGC GGTCGACTTT TGATATTTCG TGCTGTCGCC TTCTGAAAGT GACA
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| Recombination Sites |
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| Name |
Coordinates |
Gene |
Sequence |
| r6 |
3826-3840 |
15 |
CTGGAACGTT TGCGC |
| r5 |
3872-3885 |
14 |
AGTTTTTGTC ACAT |
| r4 |
3877-3890 |
14 |
TTGTCACATT ACGA |
| r3 |
3928-3941 |
14 |
GGGTTAAGTG ACAG |
| res |
3969-4003 |
35 |
ACACTGTCAC ATAATCGAAC GTATATGTGA CAGGT |
| r2 |
3972-3985 |
14 |
CTGTCACATA ATCG |
| r1 |
3988-4001 |
14 |
CGTATATGTG ACAG |
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tniA |
Tn5053.4 |
144-1823 |
Transposase |
|
+ |
| tniB |
Tn5053.4 |
1826-2734 |
Accessory Gene |
|
+ |
| tniQ |
Tn5053.4 |
2731-3948 |
Accessory Gene |
Target Site Selection |
+ |
| tniR |
Tn5053.4 |
4009-4623 |
Accessory Gene |
Resolvase |
+ |
| merE; urf-1 |
Tn5053.4 |
4676-4912 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merD |
Tn5053.4 |
4909-5274 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merA |
Tn5053.4 |
5291-6937 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merF |
Tn5053.4 |
6934-7179 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merP |
Tn5053.4 |
7182-7457 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merT |
Tn5053.4 |
7473-7823 |
Passenger Gene |
Heavy Metal Resistance |
- |
| merR |
Tn5053.4 |
7895-8329 |
Passenger Gene |
Heavy Metal Resistance |
+ |
| tnp |
IS4321 |
8429-9433 |
Transposase |
|
+ |
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniA |
TniA |
Tn5053.4 |
1680 |
144-1823 |
+ |
| Class: | Transposase |
| Transpoase Chemistry: | DDE |
Protein Sequence:
|
MTSDTPPIAA QGVATLPDEA WAQARHRTEI IGPLAALEVV GHEAADEAAQ ALGLSRRQVY VLIRRARQGT GLVTDLTPGR SGGGKGKGRL PEPVERIIRE
LLQKRFLTKQ KRSLAAFHRE VAQACKTQKL PVPARNTVAQ RIAGLHPAKI ARSRGGQDAA RPLQGAGGIP PEVTMPLEQV QIDHTVIDLI VVDERDRQPI
GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLAHAAC DKRPWLEGLN VEMDWPMSGK PRLLYLDNAA EFKSEALRRG CEQHGIRLDY RPPGQPHYGG
IVERIIGTAM QMIHDELPGT TFSNPGQRGE YDSEKMATLT LRELERWLAL AVGTYHGSVH NGLLQPPAAR WAEAVERVGV PAVVTRPTAF LVDFLPVIRR
TLTRTGFVID HIHYYADALK PWIARRERLP AFLIRRDPRD ISRIWVLEPE GQHYLEIHYR TLSHPAVTLW EQRQALAKLR QLGREQVDES ALFRMIGQMR
EIVTTAQKAT RKARRDADRR QHLKTSEPPA KPIPPDVDMA DPQADNLPPA KPFDQIEEW
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniB |
TniB |
Tn5053.4 |
909 |
1826-2734 |
+ |
| Class: | Accessory Gene |
| Function: | is needed for transposition of Tn5053 |
| Transpoase Chemistry: | Serine |
| Comment: | homologous to TnsC protein of Tn7 putative ATP-binding protein |
Protein Sequence:
|
MDEYPVIDLS HLLPAAQGLA RLPADERIQR IRADRWIGYP RAVEALNRLE TLYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRAHPVGTD ADQEHIPVLV
VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRE AYLAIRSDDQ
LENRFEPMLL PPWEANEDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLVAAAVAAV ESGEEAINHR TLSMADYIGP SERRRQFERE
LM
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniQ |
TniQ |
Tn5053.4 |
1218 |
2731-3948 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Target Site Selection |
| Function: | is needed for transposition of Tn5053 |
Protein Sequence:
|
MKSAPRWPLH PAPKEGEALS SWLNRVAACY QMDVHELLAH DLGHSQLDDL DTAPSLSLLT ALCQRSGVEL ERLRSMSLAG WVPWLLDSLD DSVPAALETY
TFQCAVLLPK RTRKVRSITR WRAWLPSQTI RRACPQCLND PTNQAVLLVW QLPLMLSCPQ HGCWLESYWG MPGRYLQWEI ADAAPRPADD AIACMDRRTW
QALTTGFVEL PRRRVHAGLW FRLLRTLLDE LNTPLSHCGS CSASIRHVWE RCGHPLRAGQ SLWRPYEILA PAVQWQMLEA AATAITLIES KALIPRGEQA
ALFQTEPYTG FTNGLPAKVP KPEPINHWQR AAQAIDEAII EARHNPETAR LLFTLASYGR RDPESLERLR ATFTKEGIPP EFLSHYEPEW PFAGLRLNDG
LSDSF
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tniR |
TniR |
Tn5053.4 |
615 |
4009-4623 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Resolvase |
| Transpoase Chemistry: | Serine |
| Sequence Family: | Serine Site-Specific Recombinase |
| Comment: | also called TniC |
Protein Sequence:
|
MLIGYMRVSK ADGSQATDLQ RDALIAAGVD PVHLYEDQAS GMREDRPGLT SCLKALRTGD TLVVWKLDRL GRDLRHLINT VHDLTGRGIG LKVLTGHGAA
IDTTTAAGKL VFGIFAALAE FERELIAERT IAGLASARAR GRKGGRPFKM TAAKLRLAMA AMGQPETKVG DLCQELGVTR QTLYRHVSPK GELRPDGEKL
LSRI
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merE; urf-1 |
MerE |
Tn5053.4 |
237 |
4676-4912 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | Broad-spectrum mercury transporter |
Protein Sequence:
|
MNSPERLPSE THKPITGYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE YWGIAALTLT GLFVLSVTRL LRAFKDRS
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merD |
MerD |
Tn5053.4 |
366 |
4909-5274 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
Protein Sequence:
|
MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVACTPGGYG LFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGDE AAAQLAVLRQ FVERRREALA
DLEVQLATMP TEPAQHAESL P
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merA |
MerA |
Tn5053.4 |
1647 |
5291-6937 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Function: | mercuric ion reductase |
| Target: | Mercury |
Protein Sequence:
|
MTEITVNGMT CTSCATHVKD ALEKIPGVNA AVVSYPESRA QVMADTAVSH NQLLAAIAAL GYQGSIRVGD FKDEPKIRDA LEGAGLHIAI IGSGGAAMAA
ALKAVEQGAT VTLIERGTIG GTCVNIGCVP SKIMIRAAHI AHLRRESPFD GGIAATVPAI DRSKLLAQQQ ARVDELRHAK YEGILDGNPA ITVLHGEARF
KDDQSLVVRL NEGGEREVTF DRCLVATGAS PAVPPIPGLK ESPYWTSTEA LVSDTIPARL AVIGSSVVAL ELAQAFARLG SQVTILARST LFFREDPAIG
EAVTAAFRAE GIEVLEHTQA SQVAHVNGEF VLTTGHGELR ADKLLVATGR APNTRSLALD AAGVTVNAQG AIVIDQGMRT SNPNIYAAGD CTDQPQFVYV
AAAAGTRAAI NMTGGDAALN LTAMPAVVFT DPQVATVGYS EAEAHHDGIE TDSRTLTLDN VPRALANFDT RGFIKLVIEE GSGRLIGVQA VAPEAGELIQ
TAVLAIRNRM TVQELADQLF PYLTMVEGLK LAAQTFNKDV KQLSCCAG
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merF |
MerF |
Tn5053.4 |
246 |
6934-7179 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | mercuric ion transport protein |
Protein Sequence:
|
MKDPKTLLRV SIIGTTLVAL CCFTPVLVIL LGVVGLSALT GYLDYVLLPA LAIFIGLTIY AIQRKRQADA CCTPKFNGVK K
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merP |
MerP |
Tn5053.4 |
276 |
7182-7457 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | periplasmic mercuric ion binding protein |
Protein Sequence:
|
MKKLLSALAL AAVVAPVWAA TQTVTLSVPG MTCSACPITV KKAISKVDGV SKVDVTFETR EAVVTFDDAK TSVQKLTKAT EDAGYPSSVK N
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merT |
MerT |
Tn5053.4 |
351 |
7473-7823 |
- |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
| Comment: | mercury ion transport protein |
Protein Sequence:
|
MSEPQNGRGA LFTGGLAAIL ASACCLGPLV LIALGFSGAW IGNLTVLEPY RPIFIGVALV ALFFAWRRIY RPSAACKPGE VCAIPQVRAT YKLIFWGVAV
LVLVALGFPY VVPFFY
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| merR |
MerR |
Tn5053.4 |
435 |
7895-8329 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Heavy Metal Resistance |
| Target: | Mercury |
Protein Sequence:
|
MENNLENLTI GVFARTAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGETD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASSLAE HKLKDVRERM
ADLARMEAVL SDLVCACHAR KGNVSCPLIA SLQGKKEPRS ADAV
|
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnp |
Tnp |
IS4321 |
1005 |
8429-9433 |
+ |
| Class: | Transposase |
| Transpoase Chemistry: | DEDD |
Protein Sequence:
|
MENIALIGID LGKNSFHIHC QDRRGKAVYR KKFTRPKLIE FLATCPATTI AMEACGGSHF MARKLEELGH SPKLISPQFV RPFVKSNKND FVDAEAICEA
ASRPSMRFVQ PRTESQQAMR ALHRVRESLV QDKVKTTNQM HAFLLEFGIS VPRGAAVISR LSTILEDNSL PLYLSQLLLK LQQHYHYLVE QIKDLESQLK
RKLDEDEVGQ RLLSIPCVGT LTASTISTEI GDGKQYASSR DFAAATGLVP RQYSTGGRTT LLGISKRGNK KIRTLLVQCA RVFIQKLEHQ SGKLADWVRD
LLCRKSNFVV TCALANKLAR IAWALTARQQ TYVA
|
|
| Internal Transposable Elements (TE) |
|
|
| TnCentral Accession |
TE Name |
Type |
Coordinates |
Length |
| IS4321-U67194 |
IS4321 |
Insertion Sequence |
8362-9688 |
1327 |
|
| Internal Repeat Elements |
|
|
| Name |
Associated Mobile Element |
Coordinates |
Sequence (Top Strand) |
| repeat t1 |
Tn5053.4 |
9-27 |
TCAGAAGACG ACCGCACCA |
| repeat t2 |
Tn5053.4 |
49-67 |
CACACGTATG CCGAGGACT |
| repeat t3 |
Tn5053.4 |
78-96 |
TCAGGAGTCG TCTGCACCA |
| repeat t4 |
Tn5053 |
110-128 |
TCAATACTCG TGTGCACCA |
| IR |
Tn5053.4 |
8346-8383 |
GAATCGCACG AAATAAATTA CTCTACCAGT GAGGGAGG |
| IRL |
IS4321 |
8362-8380 |
TAATGAGATG GTCACTCCC |
| IRR |
IS4321 |
9674-9688 |
CCCTCACTGG TATCT |
| repeat i2 |
Tn5053.4 |
9725-9743 |
CCGTGCGGTC GACTTTTGA |
| IRi |
Tn5053.4 |
9747-9774 |
AAGCACGACA GCGGAAGACT TTCACTGT |
|
| References |
|
|
Ren Y, Ren Y, Zhou Z, Guo X, Li Y, Feng L, Wang L. Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047. J Bacteriol. 2010 May;192(9):2463-4. doi: 10.1128/JB.00067-10. Epub 2010 Mar 5. PubMed ID: 20207761
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