Transposon
Name: In28
Family: Tn402        Group: Class 1
Evidence of Transposition: yes
 Host     

Host Organism:Pseudomonas aeuroginosa RPL11 Molecular Source:plasmid RPL11
Place of Origin:Sydney, Australia Date of Isolation:2006

 Map     



 Terminal Inverted Repeats (IR)     


 Sequence     
DNA SequenceLength  6266 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TGTCATTTTC AGAAGACGAC TGCACCAGTT GATTGGGCGT AATGGCTGTT GTGCAGCCAG CTCCTGACAG TTCAATATCA GAAGTGATCT GCACCAATCT 100
CGACTATGCT CAATACTCGT GTGCACCAAA GCGAGGTGAG CATGGCGACG GAGGCTCTGT TGCAAAGATT GGCGGCAGTC AGAGGTAGGC TGTCGCTCTG 200
CGCCGATCAG GCGGCTGCTG CGAAATGGTG GTTGAGCATG CCCATGGCCT CCGTCAGCGC CGAGGGCCCA ATGCCAAAAG CTCTCTCCAC AAGGCGCACC 300
TCGCCCCTGA TGCCGGGCTG CAGGCACCAG GGGCGAGCCT GTCCTTTGCG CAGGGCTCGC ATGACTTCGA ATCCCTTGAT CGTGGCATAG GCCGTGGGGA 400
TCGATTTGAA ACCGCGCACC GGCTTGATCA GTATCTTGAG CTTTCCGTGA TCGGCCTCGA TCACGTTATT GAGATACTTC ACCTGCCGGT GGGCCGTCTC 500
CCGGTCCAGC TTTCCTTCGC GCTTCAATTC GGTGATCGCT GCACCATAGC TCGGCGCTTT GTCGGTATTG AGCGTGGCAG GCTTTTCCCA GTGCTTCAGG 600
CCTCGCAGGG CCTTGCCCAG GAACCGCTTC GCTGCCTTGG CGCTGCGGGT CGGCGACAGG TAGAAATCGA TCGTGTCGCC CCGCTTGTCG ACTGCCCGGT 700
ACAGGTAGGT CCACTTGCCC CGCACCTTGA CGTAGGTTTC ATCCAGGCGC CAGCTCGGAT CAAAGCCACG CCGCCAGAAC CAGCGCAGCC GCTTCTCCAT 800
CTCCGGGGCG TAGCACTGGA CCCAGCGATA GATCGTCGTA TGGTCGACCG AAATGCCGCG TTCCGCCAGC ATTTCCTCAA GGTCGCGATA GCTGATCGGA 900
TAGCGACAAT ACCAGCGCAC CGCCCACAGG ATCACATCAC CCTGGAAATG GCGCCACTTG AAATCCGTCA TCGTTCCGTC CGTCCAATCT CCGCCAAGCA 1000
TGCTCAAGCT TCACGATTTT TGCAACAGAG CCGCGACTTC CACGATGGGG ATCGGGCGAG CAAAAAGGCA GCAATTATGA GCCCCATACC TACAAAGCCC 1100
CACGCATCAA GCTTTTGCCC ATGAAGCAAC CAGGCAATGG CTGTAATTAT GACGACGCCG AGTCCCGACC AGACTGCATA AGCAACACCG ACAGGGATGG 1200
ATTTCAGAAC CAGAGAAAGA AAATAAAATG CGATGCCATA ACCGATTATG ACAACGGCGG AAGGGGCAAG CTTAGTAAAG CCCTCGCTAG ATTTTAATGC 1300
GGATGTTGCG ATTACTTCGC CAACTATTGC GATAACAAGA AAAAGCCAGC CTTTCATGAT ATATCTCCCA ATTTGTGTAG GGCTTATTAT GCACGCTTAA 1400
AAATAATAAA AGCAGACTTG ACCTGATAGT TTGGCTGTGA GCAATTATGT GCTTAGTGCA TCTAACGCTT GAGTTAAGCC GCGCCGCGAA GCGGCGTCGG 1500
CTTGAACGAA TTGTTAGACA TTATTTGCCG ACTACCTTGG TGATCTCGCC TTTCACGTAG TGAACAAATT CTTCCAACTG ATCTGCGCGG GAGGCCAAGC 1600
GATCTTCTTC TTGTCCAAGA TAAGCCTGTC TAGCTTCAAG TATGACGGGC TGATACTGGG CCGGCAGGCG CTCCATTGCC CAGTCGGCAG CGACATCCTT 1700
CGGCGCGATT TTGCCGGTTA CTGCGCTGTA CCAAATGCGG GACAACGTAA GCACTACATT TCGCTCATCG CCAGCCCAGT CGGGCGGCGA GTTCCATAGC 1800
GTTAAGGTTT CATTTAGCGC CTCAAATAGA TCCTGTTCAG GAACCGGATC AAAGAGTTCC TCCGCCGCTG GACCTACCAA GGCAACGCTA TGTTCTCTTG 1900
CTTTTGTCAG CAAGATAGCC AGATCAATGT CGATCGTGGC TGGCTCGAAG ATACCTGCAA GAATGTCATT GCGCTGCCAT TCTCCAAATT GCAGTTCGCG 2000
CTTAGCTGGA TAACGCCACG GAATGATGTC GTCGTGCACA ACAATGGTGA CTTCTACAGC GCGGAGAATC TCGCTCTCTC CAGGGGAAGC CGAAGTTTCC 2100
AAAAGGTCGT TGATCAAAGC TCGCCGCGTT GTTTCATCAA GCCTTACGGT CACCGTAACC AGCAAATCAA TATCACTGTG TGGCTTCAGG CCGCCATCCA 2200
CTGCGGAGCC GTACAAATGT ACGGCCAGCA ACGTCGGTTC GAGATGGCGC TCGATGACGC CAACTACCTC TGATAGTTGA GTCGATACTT CGGCGATCAC 2300
CGCTTCCCTC ATGATGTTTA ACGCCTGAGC TCAGCCGACC GAAACCGCGT AGCGGTTTTG GGTCGGCTGC AGCGATTTGT TGGGCGATAG CTTGCCACAT 2400
TCTCTCAACG ATTGGGATTT GATGTACTTT CCGCACTTTG TAGCGCGACC ACATCATGCT CCCCCTGGCC GCGAGAGCCC TTCACTCGGG AAACACAAGA 2500
CAGACCGAGC ACGACTGTTG CAAGGGTCAA ACAGTACACG ACAACCGGCC AAGCCGTGTT GCGCGGCAAC AGCGAAATGA TCAACGTTCC GATGCTTCCT 2600
AGCAGTACAC CGCCCAAGCA GAAGTAGACT GCCGTGACCG TTCCAGCAAC ATGGTCGAAT CCTCGAAGAG CGCCATTGGG CGCCACAGAT ACCGCTGTGG 2700
CGACACCAAT ACCCACTAGC CACATTGGAG CAATAAAGCC TAACACGGAC TGCAAAGCCC ATATTTCGGT GATGGCAAGC AATACTGCTC CAGCTATCAG 2800
GCATCCCATT CCCATTCGCA AGACACTTGG GCTGCCCCAC TTGGGTATCA CACGCCCCAT AAAACGAGCC GTAAACACCA TGGCAATTGC CACTGTGGCG 2900
AACAGCAGGC TGAAGCCAAG CTGAGACACA CCTTGCCTGC CCATCATTAG TCCGGGCGCA ATGGAGAAAA AGACGAAGAA GCTACCCATT CCAGCGGCGT 3000
AACACAACGT GTACAACCAG AAGTTCAGGC ACTTAACGGG GAGTAGCAGC TGCGACCATT GCAAGCCCGC AACTCGTTGC ACCCGGGTTT CAGGCCAGAA 3100
TCGCCACGCT GCTGCAGATG CAGCGATCAT GCCCAAACCT AGAAACGCAA AGATAGCCCG CCACCCAAGC CACATGTCGA CGAGCGCTCC GAGCAATGGG 3200
CCTACCGCCG GGACTATGGC CAGCATGGAT CCGAGTATGC CGTAAATGAC ATTACTTTCC TCGCGACCTG CGTAAATGTC ACGTACTGTT GCAAATGTGG 3300
AAACAAGGCA CGCCGAGGCA CCACAAGCCT GAAGAATCCG AAGCCCCAGA AAGACTTCAG CCGATGACGT AAGAGCGAGG CCCATTGACG CCACAACGTA 3400
GGCGAGGCCA CCTCCCAGTA GAACGGGGCG GCGCCCCAGT CGGTCCGATA GCGGTCCAAA CAAGAGCTGA CCGGCACCAA TCATGACCAA GTACGTTGTC 3500
AGCGTAAGCT GAATTGTGCT CGCTGTCGTA CCAAGCGCGT TTGGCATAAA CGGCACTGCT GGCAAGTACA TGTCCATGCC GAGTGATGCC AATAAATCGA 3600
ACGGTGATAA CAACAACACC GTGGCGGCAA GGGAGTACCG CCAACTAAAG TTTTTTGAGC GCACGAAACC ATCTCCTTGA ACAAAGGATT TGGCGGCGCT 3700
CCCGTAGAGC AAAAGGATTC ATGAGAACGC CGCAACAACC GAAAAATGAA GGTTGCTGCG GCTTACTTGT CTGCGTTCTT GGAAGTGCTC ATCTGCTGAC 3800
TATCTCATGA TTAAATTTGA ATCGTAACAA CTTTCAAGCA ACTCTGCAAG GAGCCTTATT GTGCGCCCAA CGCCGCCATA AGTTGCGGCT TTGGAGTTGA 3900
TTGTTTTGTG GTAGCGTAGC GTTAAACCAC AAAACAAGCG ACGGAAAAGC CGTCAACTTG ATGGCCTTGT TAGCCTTATC AGCGCGACTG TGATGTATAA 4000
ACGTCAAAAA TTGAATGACC AATTTTAACA ATCGCATCAT TTCGCTCTGC CATTGAAGCC TGTGTTTGAG CTAGATAGAT GCTCACAATA ATTGGGGCTT 4100
GATGCTCACT CCACACAACT GCTGTAATAC TCCGAGCACC AAATCCGCCA GCACCTGAGC GATCCGCAAT GTTCCATCCC GCCGGCAATA CTGAACGTAG 4200
TAAATTACCA GTGACTTGAT TGTTCACCAT CCAAGACTCT AATTTTTTCT GGTTCATTTC AGATAGCGCG GAACCAAATA AAAATTTATT CAAAGTACTG 4300
GCTATTGCCT TAGGAGTTGT CGTATCCCTC AAATCACCGA GCTTACCTTC ATTTAAATCA GGCTCAATAC GGTCTAGACG AGTCTCTTTG TCCCCAATTT 4400
GTCTTAAAAA ATCAGTAACG CCTTTGGGGC CACCTACAGC ACTTAGGATG ATATTTGCCG CAGTATTATC ACTTGTAGTC ATAGTTGCGA AGCACGCATC 4500
ATCGAGTGTG ATTGCCTGCC CTACTTGCTT TTCTATTACA GGGGAATAGG TCACAAGATC TGCTTTCTTA ATCTCGACTG TACTATTGGG ATTAACTTTT 4600
CCTTGCTCAG CATCATATAG TAATTTAGCG CAAGCTATTG TTTTAAAAGT ACTTGTTAAC GGGAAGCGCT GATTGCCATT GTAATCCCAA TATTCTCCAT 4700
TTTGAGTATC AAGAACGGAA ACACCTATAC GAGCAGAAAG AGAAACTTCA ATTGCCTTAA CGTCTTGTTC AACTTGCTGA AACTTTGAAC TACTTGCAAA 4800
AACCACGGAT GGTATTAAAA GCGAAAATGC CAATAAAAAC TTCATGTAAG TAACCCCATA ATTTTGATTG TCACACATTT TATATAAAAG CATGAGGCTC 4900
CATAATATGG CTAACTTTGT TTTAGGGCGA CTGCCCTGCT GCGTAACATC GTTGCTGCTC CATAACATCA AACATCGACC CACGGCGTAA CGCGCTTGCT 5000
GCTTGGATGC CCGAGGCATA GACTGTACAA AAAAACAGTC ATAACAAGCC ATGAAAACCG CCACTGCGCC GTTACCACCG CTGCGTTCGG TCAAGGTTCT 5100
GGACCAGTTG CGTGAGCGCA TACGCTACTT GCATTACAGC TTACGAACAG AACAGGCTTA TGTCCACTGG GTTCGTGCCT TCATCCGTTT CCACGGTGTG 5200
CGTCACCCGG CAACCTTGGG CAGCAGCGAA GTCGAGGCAT TTCTGTCCTG GCTGGCGAAC GAGCGCAAGG TTTCGGTCTC CACGCATCGT CAGGCATTGG 5300
CGGCCTTGCT GTTCTTCTAC GGCAAGGTGC TGTGCACGGA TCTGCCCTGG CTTCAGGAGA TCGGAAGACC TCGGCCGTCG CGGCGCTTGC CGGTGGTGCT 5400
GACCCCGGAT GAAGTGGTTC GCATCCTCGG TTTTCTGGAA GGCGAGCATC GTTTGTTCGC CCAGCTTCTG TATGGAACGG GCATGCGGAT CAGTGAGGGT 5500
TTGCAACTGC GGGTCAAGGA TCTGGATTTC GATCACGGCA CGATCATCGT GCGGGAGGGC AAGGGCTCCA AGGATCGGGC CTTGATGTTA CCCGAGAGCT 5600
TGGCACCCAG CCTGCGCGAG CAGCTGTCGC GTGCACGGGC ATGGTGGCTG AAGGACCAGG CCGAGGGCCG CAGCGGCGTT GCGCTTCCCG ACGCCCTTGA 5700
GCGGAAGTAT CCGCGCGCCG GGCATTCCTG GCCGTGGTTC TGGGTTTTTG CGCAGCACAC GCATTCGACC GATCCACGGA GCGGTGTCGT GCGTCGCCAT 5800
CACATGTATG ACCAGACCTT TCAGCGCGCC TTCAAACGTG CCGTAGAACA AGCAGGCATC ACGAAGCCCG CCACACCGCA CACCCTCCGC CACTCGTTCG 5900
CGACGGCCTT GCTCCGCAGC GGTTACGACA TTCGAACCGT GCAGGATCTG CTCGGCCATT CCGACGTCTC TACGACGATG ATTTACACGC ATGTGCTGAA 6000
AGTTGGCGGT GCCGGAGTGC GCTCACCGCT TGATGCGCTG CCGCCCCTCA CTAGTGAGAG GTAGGGCAGC GCAAGTCAAT CCTGGCGGAT TCACTACCCC 6100
TGCGCGAAGG CCATCGGTGC CGCATCGAAC GGCCGGTTGC GGAAAGTCCT CCCTGCGTCC GCTGATGGCC GGCAGCAGCC CGTCGTTGCC TGATGGATCC 6200
AACCCCTCCG CTGCTATAGT GCAGTCGGCT TCTGACGTTC AGTGCAGCCG TCTTCTGAAA ACGACA

 Recombination Sites     

Name Coordinates Gene Sequence
attC aadA1a core 1466-1519 54 CGCTTGAGTT AAGCCGCGCC GCGAAGCGGC GTCGGCTTGA ACGAATTGTT AGAC
attC cmlA6 core 2322-2385 64 CGCCTGAGCT CAGCCGACCG AAACCGCGTA GCGGTTTTGG GTCGGCTGCA GCGATTTGTT
GGGC
attC bla CARB-1 core 3871-3975 105 CGCCGCCATA AGTTGCGGCT TTGGAGTTGA TTGTTTTGTG GTAGCGTAGC GTTAAACCAC
AAAACAAGCG ACGGAAAAGC CGTCAACTTG ATGGCCTTGT TAGCC
attI 4915-4970 56 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnp IS6100 207-971 Transposase   -
qacEdelta1 (ARO:3005010) In28 1004-1357 Passenger Gene Antibiotic Resistance -
aadA (ARO:3002601) In28 1521-2312 Passenger Gene Antibiotic Resistance -
cmlA6 (ARO:3002696) In28 2405-3664 Passenger Gene Antibiotic Resistance -
bla CARB-1 (ARO:3002240) In28 3979-4845 Passenger Gene Antibiotic Resistance -
intI1 In28 5051-6064 Integron Integrase Class 1 +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS6100 765 207-971 -
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MTDFKWRHFQ GDVILWAVRW YCRYPISYRD LEEMLAERGI SVDHTTIYRW VQCYAPEMEK RLRWFWRRGF DPSWRLDETY VKVRGKWTYL YRAVDKRGDT
IDFYLSPTRS AKAAKRFLGK ALRGLKHWEK PATLNTDKAP SYGAAITELK REGKLDRETA HRQVKYLNNV IEADHGKLKI LIKPVRGFKS IPTAYATIKG
FEVMRALRKG QARPWCLQPG IRGEVRLVER AFGIGPSALT EAMGMLNHHF AAAA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
qacEdelta1 (ARO:3005010) QacEdelta1 In28 354 1004-1357 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic efflux (ARO:0010000)
Target:   disinfecting agents and antiseptics (ARO:3005386)
Sequence Family:  major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Comment:   perfect match to first 108 amino acids of reference sequence for ARO:3005010 (bitscore: 204)||Synonyms:
Protein Sequence:  
MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL
ARSPSWKSRL CCKNREA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
aadA (ARO:3002601) AadA In28 792 1521-2312 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Transpoase Chemistry:   aminoglycoside nucleotidyltransferase
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  ANT(3'') (ARO:3004275)
Comment:   perfect match to reference sequence for ARO:3002601||Synonyms: aadA1-pm aadA, aadA1, aad(3'')(9)
Protein Sequence:  
MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR
YPAKRELQFG EWQRNDILAG IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA GDERNVVLTL SRIWYSAVTG
KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA SRADQLEEFV HYVKGEITKV VGK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
cmlA6 (ARO:3002696) CmlA6 In28 1260 2405-3664 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic efflux (ARO:0010000)
Target:   phenicol antibiotic (ARO:3000387)
Sequence Family:  major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Comment:   strict match to reference sequence for ARO:3002696 (bitscore: 819)
Protein Sequence:  
MRSKNFSWRY SLAATVLLLS PFDLLASLGM DMYLPAVPFM PNALGTTAST IQLTLTTYLV MIGAGQLLFG PLSDRLGRRP VLLGGGLAYV VASMGLALTS
SAEVFLGLRI LQACGASACL VSTFATVRDI YAGREESNVI YGILGSMLAI VPAVGPLLGA LVDMWLGWRA IFAFLGLGMI AASAAAWRFW PETRVQRVAG
LQWSQLLLPV KCLNFWLYTL CYAAGMGSFF VFFSIAPGLM MGRQGVSQLG FSLLFATVAI AMVFTARFMG RVIPKWGSPS VLRMGMGCLI AGAVLLAITE
IWALQSVLGF IAPMWLVGIG VATAVSVAPN GALRGFDHVA GTVTAVYFCL GGVLLGSIGT LIISLLPRNT AWPVVVYCLT LATVVLGLSC VSRVKGSRGQ
GEHDVVALQS AESTSNPNR

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
bla CARB-1 (ARO:3002240) Bla CARB-1 In28 867 3979-4845 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   penam (ARO:3000008)
Sequence Family:  CARB beta-lactamase (ARO:3000091)
Comment:   strict match to reference sequence for ARO:3002240 (bitscore: 593)||Synonyms: NDM-1, PSE-4, BlaP1a
Protein Sequence:  
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY
SPVIEKQVGQ AITLDDACFA TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA IASTLNKFLF GSALSEMNQK
KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI TAVVWSEHQA PIIVSIYLAQ TQASMAERND AIVKIGHSIF DVYTSQSR

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
intI1 IntI1 In28 1014 5051-6064 +
Class:   Integron Integrase
Sub Class:   Class 1
Transpoase Chemistry:   Tyrosine
Sequence Family:  Class 1 Integron Tyrosine Integrase
Protein Sequence:  
MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW
LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL
KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL
LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
IS6100-U12338.3 IS6100 Insertion Sequence 153-1032 880

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
repeat t1 In28 9-27 TCAGAAGACG ACTGCACCA
repeat t2 In28 49-67 AACACGTCGG TCGAGGACT
repeat t3 In28 78-97 TCAGAAGTGA TCTGCACCAA
repeat t4 Tn402 110-128 TCAATACTCG TGTGCACCA
IR IS6100 153-166 GGCTCTGTTG CAAA
IR IS6100 1019-1032 AAACGTTGTC TCGG
repeat i4 In28 6147-6165 AGGAGGGACG CAGGCGACT
repeat i3 In28 6175-6193 CGTCGGGCAG CAACGGACT
repeat i2 In28 6217-6235 ATCACGTCAG CCGAAGACT
IRi In28 6234-6266 CTGCAAGTCA CGTCGGCAGA AGACTTTTGC TGT

 References     

1.Partridge SR, Recchia GD, Stokes HW, Hall RM. Family of class 1 integrons related to In4 from Tn1696. Antimicrob Agents Chemother. 2001 Nov;45(11):3014-20. doi: 10.1128/AAC.45.11.3014-3020.2001. PubMed ID: 11600350
2.Stokes HW, Elbourne LD, Hall RM. Tn1403, a multiple-antibiotic resistance transposon made up of three distinct transposons. Antimicrob Agents Chemother. 2007 May;51(5):1827-9. doi: 10.1128/AAC.01279-06. Epub 2007 Jan 29. PubMed ID: 17261631